FastQCFastQC Report
Wed 25 May 2016
SRR1295004_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295004_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3242161
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC147740.45568372452817735No Hit
GTACATGGGAAGCAGTGGTATCAAC143180.4416190312572386No Hit
GTATCAACGCAGAGTACTTTTTTTT121940.3761071704952345No Hit
GGTATCAACGCAGAGTACTTTTTTT107830.33258681478186924No Hit
TATCAACGCAGAGTACTTTTTTTTT84600.2609370725266265No Hit
GAGTACATGGGAAGCAGTGGTATCA75000.23132719195622917No Hit
CATGTACTCTGCGTTGATACCACTG56980.17574697863554586No Hit
ACGCAGAGTACTTTTTTTTTTTTTT50430.1555444038713685No Hit
ACGCAGAGTACATGGGAAGCAGTGG45570.14055440183260487No Hit
GCTTCCCATGTACTCTGCGTTGATA44860.13836450441541923No Hit
GCGTTGATACCACTGCTTCCCATGT43940.13552689086075614No Hit
GTATCAACGCAGAGTACATGGGAAG41780.12886466773241675No Hit
GGTATCAACGCAGAGTACATGGGAA39490.12180147747135321No Hit
ATTCCATTCCATTCCATTCCATTCC38260.11800771152327107No Hit
TATCAACGCAGAGTACATGGGAAGC36190.11162308102527913No Hit
ACTCTGCGTTGATACCACTGCTTCC33270.10261674235178327No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCGA508.7206485E-515.1995797
CGCCGAG551.9616456E-413.81780058
AACGCCG604.0956447E-412.6661216
GGACCGT550.00307067212.0903896
CAGTGCG658.03356E-411.6912649
GACCGTG1001.9321596E-611.3996857
GTCCTAC1803.6379788E-1211.0972331
AGAACCG600.005879876711.0828565
CAACGCC700.001492694710.8566765
CCTATAC2800.010.8566763
TACACCG1002.4017198E-510.449555
ACCGTTT856.598734E-410.0585468
GTCTAAG2950.09.9955711
CTTAGAC3200.09.7964533
ACACCGT1502.6525413E-79.4995926
AGACCGT800.0045192869.4995916
CTAAGAC4150.09.38513853
GGACTGA3350.09.3578076
GTCCTAG4000.09.2741161
CGTGCAC1651.0682015E-79.21129810
Produced by FastQC (version 0.11.5)