FastQCFastQC Report
Wed 25 May 2016
SRR1295003_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295003_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2386332
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC1986428.324156068811883No Hit
CCCATGTACTCTGCGTTGATACCAC1888717.91469921201241No Hit
GAGTACATGGGAAGCAGTGGTATCA936283.9235110621657No Hit
CATGTACTCTGCGTTGATACCACTG710432.9770794675677985No Hit
GCGTTGATACCACTGCTTCCCATGT594862.492779713803444No Hit
ACGCAGAGTACATGGGAAGCAGTGG492422.063501641850338No Hit
TATCAACGCAGAGTACATGGGAAGC489082.049505265822191No Hit
GTATCAACGCAGAGTACATGGGAAG486522.038777504555108No Hit
ACTCTGCGTTGATACCACTGCTTCC433861.818104102865821No Hit
GGTATCAACGCAGAGTACATGGGAA418611.7541984937552695No Hit
GCTTCCCATGTACTCTGCGTTGATA362411.5186906096888446No Hit
GTGGTATCAACGCAGAGTACATGGG213470.8945528115953689No Hit
GTACTCTGCGTTGATACCACTGCTT212230.8893565522316257No Hit
ATACCACTGCTTCCCATGTACTCTG207370.8689905679511485No Hit
CAGTGGTATCAACGCAGAGTACATG205070.8593523449377539No Hit
GGGAAGCAGTGGTATCAACGCAGAG190470.7981705814614227No Hit
ACATGGGAAGCAGTGGTATCAACGC183760.7700521134527802No Hit
GCAGTGGTATCAACGCAGAGTACAT179430.7519071109971287No Hit
CATGGGAAGCAGTGGTATCAACGCA177870.745369881475No Hit
TACCACTGCTTCCCATGTACTCTGC174540.7314154107643027No Hit
CTGCTTCCCATGTACTCTGCGTTGA174210.7300325352884678No Hit
GTTGATACCACTGCTTCCCATGTAC170050.7125999232294584No Hit
GATACCACTGCTTCCCATGTACTCT168870.7076550957704125No Hit
GCAGAGTACATGGGAAGCAGTGGTA162260.6799556809362653No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA159100.6667136006222102No Hit
CCATGTACTCTGCGTTGATACCACT153950.6451323621356961No Hit
CTTCCCATGTACTCTGCGTTGATAC152050.6371703518202831No Hit
CCACTGCTTCCCATGTACTCTGCGT145390.6092614103988883No Hit
AAGCAGTGGTATCAACGCAGAGTAC142700.5979888800049616No Hit
ATGGGAAGCAGTGGTATCAACGCAG123020.5155192152642634No Hit
TTCCCATGTACTCTGCGTTGATACC123010.5154773099468137No Hit
GTACATGGTAAGCAGTGGTATCAAC120450.5047495486797311No Hit
TGATACCACTGCTTCCCATGTACTC103540.4338876568725559No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA101180.423998001954464No Hit
ACCATGTACTCTGCGTTGATACCAC91920.3851936779961883No Hit
ATCAACGCAGAGTACATGGGAAGCA90150.3777764368076194No Hit
CCCCATGTACTCTGCGTTGATACCA74070.31039268634875616No Hit
TCCATGTACTCTGCGTTGATACCAC68480.2869676138944623No Hit
GTATCAACGCAGAGTACTTTTTTTT62020.25989677882205825No Hit
AAAAAGTACTCTGCGTTGATACCAC60850.25499385668046193No Hit
GGAAGCAGTGGTATCAACGCAGAGT58160.24372132628653514No Hit
GTACATGGGTAAGCAGTGGTATCAA57330.24024318493822316No Hit
GGTAAGCAGTGGTATCAACGCAGAG56650.23739362335165434No Hit
GAACAAAAAAAAAAAAAAAAAAAAA53790.2254087025610854No Hit
GTACTTTTTTTTTTTTTTTTTTTTT52860.221511508038278No Hit
TATCAACGCAGAGTACTTTTTTTTT47490.19900835256787403No Hit
GGTATCAACGCAGAGTACTTTTTTT47030.1970807079651951No Hit
CTGCGTTGATACCACTGCTTCCCAT46060.19301589217258958No Hit
GAGTACATGGTAAGCAGTGGTATCA44630.18702343177730507No Hit
TCCCATGTACTCTGCGTTGATACCA43960.18421577550818577No Hit
CTCTGCGTTGATACCACTGCTTCCC43530.1824138468578555No Hit
TGGGAAGCAGTGGTATCAACGCAGA42830.17948047463638755No Hit
GTACATGGAAAGCAGTGGTATCAAC42810.1793966640014885No Hit
AAAGTACTCTGCGTTGATACCACTG41490.17386516209814898No Hit
TCAACGCAGAGTACATGGGAAGCAG38430.16104213495858916No Hit
GCTTACCATGTACTCTGCGTTGATA37640.15773161488007537No Hit
GTACATGGAAGCAGTGGTATCAACG37320.15639064472169004No Hit
AGTGGTATCAACGCAGAGTACATGG34070.14277141655058895No Hit
TTGATACCACTGCTTCCCATGTACT30710.1286912298875429No Hit
GAGTACATGGAAAGCAGTGGTATCA30700.12864932457009334No Hit
ACTCTGCGTTGATACCACTGCTTAC30670.12852360861774473No Hit
GCGTTGATACCACTGCTTACCATGT29770.12475213004728596No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN28380.11892729092179966No Hit
AACGCAGAGTACATGGGAAGCAGTG27680.11599391870033171No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26990.11310245179631333No Hit
ACTGCTTCCCATGTACTCTGCGTTG26690.11184529227282708No Hit
GAGTACTTTTTTTTTTTTTTTTTTT26440.11079765933658854No Hit
GCTTTCCATGTACTCTGCGTTGATA26410.11067194338423993No Hit
ACGCAGAGTACATGGTAAGCAGTGG25240.10576902124264352No Hit
ATGGTAAGCAGTGGTATCAACGCAG24740.10367375537016645No Hit
CTGCTTACCATGTACTCTGCGTTGA24200.10141086822789117No Hit
ATGTACTCTGCGTTGATACCACTGC24070.10086609910104713No Hit
CGTTGATACCACTGCTTCCCATGTA23870.10002799275205629No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCGA250.00603535118.9987187
GTACAAA9750.016.2884831
TACATAA8050.014.9938082
GTACATA11500.014.9675091
GGTGTAA400.005244406414.2646151
TTTAAGG400.00526565414.2559252
AACTATA400.005282556614.249047
GCGGTTC400.0052899214.24604811
GCGAATG400.00529139414.24544914
TACAAAA10300.014.0252462
CCAGTAC551.9539396E-413.8239283
TTAAGCT500.00149337313.3077664
GCTTAGG500.001495265813.305532
AGCCCTA500.001503570713.295753513
AGAACAA14500.012.9778072
GTAAAAA4500.012.89098551
CTACACT604.0733392E-412.6740634
ACTCTGG751.4803918E-512.66581258
ATGTACG1155.2823452E-912.398544
CGTTTAT701.0946289E-412.21038614
Produced by FastQC (version 0.11.5)