##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295003_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2386332 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.0395548481938 33.0 33.0 33.0 15.0 33.0 2 29.844138200384524 33.0 27.0 33.0 15.0 33.0 3 30.20118365759668 33.0 33.0 33.0 22.0 33.0 4 33.1322573724025 37.0 33.0 37.0 15.0 37.0 5 34.20225517656387 37.0 33.0 37.0 27.0 37.0 6 33.61570058147819 37.0 33.0 37.0 22.0 37.0 7 33.83109475127518 37.0 33.0 37.0 27.0 37.0 8 33.582379568308184 37.0 33.0 37.0 22.0 37.0 9 33.42272910894209 37.0 33.0 37.0 22.0 37.0 10 33.62581862037638 37.0 33.0 37.0 22.0 37.0 11 33.81495827068488 37.0 33.0 37.0 27.0 37.0 12 33.84317144471096 37.0 33.0 37.0 27.0 37.0 13 33.43139512859066 37.0 33.0 37.0 22.0 37.0 14 34.92123183194962 37.0 33.0 40.0 22.0 40.0 15 34.802988435808594 37.0 33.0 40.0 22.0 40.0 16 34.826809932565965 37.0 33.0 40.0 22.0 40.0 17 34.92138562446466 37.0 33.0 40.0 22.0 40.0 18 34.75462383272738 37.0 33.0 40.0 22.0 40.0 19 34.61072642029693 37.0 33.0 40.0 22.0 40.0 20 34.781793564348966 37.0 33.0 40.0 22.0 40.0 21 34.68298250201565 37.0 33.0 40.0 22.0 40.0 22 34.769149053861746 37.0 33.0 40.0 22.0 40.0 23 34.79249199189383 37.0 33.0 40.0 22.0 40.0 24 34.926789734202956 37.0 33.0 40.0 22.0 40.0 25 33.74275792303837 37.0 33.0 40.0 15.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.680168012729947 1101 2 0.5778356015155381 1101 3 0.5565141246380492 1101 4 0.6196721220061008 1101 5 0.4922682321644416 1101 6 0.42538908388024765 1101 7 0.40431580638663434 1101 8 0.36978486786672704 1101 9 0.38003348402961024 1101 10 0.6719724367684137 1101 11 0.6375506124162555 1101 12 0.5261287716979481 1101 13 0.5369866883980663 1101 14 1.070914186663117 1101 15 0.9356187433227774 1101 16 0.9522465980900066 1101 17 0.7233297748703293 1101 18 0.7712439987496111 1101 19 0.7468184979891461 1101 20 0.6209545756391464 1101 21 0.6102651957191298 1101 22 0.6917033994944504 1101 23 0.7564790278295632 1101 24 0.8838115442298928 1101 25 0.9262353375971841 1102 1 0.5903800502644643 1102 2 0.5640490265729419 1102 3 0.42528254337960547 1102 4 0.6577659321189486 1102 5 0.45991550623531907 1102 6 0.28696582633112655 1102 7 0.419894499223453 1102 8 0.5168355189969418 1102 9 0.4217989938520432 1102 10 0.6878260162145082 1102 11 0.5271110496882656 1102 12 0.6464392626680464 1102 13 0.4544659662911741 1102 14 0.9645574635732999 1102 15 0.9635411538998397 1102 16 0.8714751544057648 1102 17 0.879675401347022 1102 18 0.9321091894774867 1102 19 0.9439151718016774 1102 20 0.9151028823197791 1102 21 0.9563510067506442 1102 22 0.7600811460549508 1102 23 0.8919207771555833 1102 24 0.903833642257311 1102 25 1.1731935863671836 1103 1 0.45849878137724076 1103 2 0.34202567516671323 1103 3 0.3190034196710698 1103 4 0.4706619856759531 1103 5 0.30380548920456363 1103 6 0.16025386897607774 1103 7 0.13432007961168324 1103 8 0.30788869246573114 1103 9 0.38608784536020124 1103 10 0.4477124844973588 1103 11 0.4011426348282825 1103 12 0.39485114249422537 1103 13 0.4156203378298926 1103 14 0.809259384642111 1103 15 0.6607096839104258 1103 16 0.6084327234514575 1103 17 0.5863092015572278 1103 18 0.6523739926589514 1103 19 0.6416823918910026 1103 20 0.5282676483282813 1103 21 0.6539417113309156 1103 22 0.5434828209743827 1103 23 0.6672807744696243 1103 24 0.6484837638361043 1103 25 0.7591820279255543 1104 1 0.5041100047126896 1104 2 0.4563575603713588 1104 3 0.4424786902251121 1104 4 0.4787889920683881 1104 5 0.38712771281147695 1104 6 0.3363833658793638 1104 7 0.21805384612774503 1104 8 0.356731960631663 1104 9 0.3075279911455766 1104 10 0.5478802735684098 1104 11 0.5358716557200438 1104 12 0.4674920081898719 1104 13 0.3975728287754663 1104 14 0.831654499969126 1104 15 0.730858831182573 1104 16 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25 0.6406360461855627 2209 1 0.13966151422663842 2209 2 0.2403527626000148 2209 3 0.22023301581186416 2209 4 0.35676280032997454 2209 5 0.2617104585555481 2209 6 0.30436647612517476 2209 7 0.31358228564679536 2209 8 0.3572165259906299 2209 9 0.5107012416461032 2209 10 0.42171718991588847 2209 11 0.41597353899893363 2209 12 0.32245712873231014 2209 13 0.37730640658173087 2209 14 0.5308410685960183 2209 15 0.5007168371987305 2209 16 0.4626296001764345 2209 17 0.46687740423836743 2209 18 0.6134952207146895 2209 19 0.5251039735522838 2209 20 0.4669764238575027 2209 21 0.47332246664227284 2209 22 0.3549381198384509 2209 23 0.63983312956357 2209 24 0.6269121099049997 2209 25 0.5802480840533164 2210 1 0.19687785853707496 2210 2 0.16418906692246438 2210 3 0.25229541025281677 2210 4 0.3202111389342477 2210 5 0.3081774179246324 2210 6 0.23735115044353705 2210 7 0.16504397684310845 2210 8 0.2823366748094145 2210 9 0.36920659546389345 2210 10 0.4512580065885956 2210 11 0.2247949485650622 2210 12 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9 -0.06763762284123231 2213 10 -1.5200922006657152 2213 11 -0.2754182802758365 2213 12 -0.009179722140629565 2213 13 -0.09214024013770938 2213 14 -0.08708118565613887 2213 15 -0.07251298081753532 2213 16 0.016804024002261997 2213 17 -0.11903686660777169 2213 18 0.06574337306095401 2213 19 0.019843538762422952 2213 20 0.05986302209100813 2213 21 0.07183602218023566 2213 22 0.15999951642922383 2213 23 -0.7562104327670056 2213 24 -1.3619698017368336 2213 25 -1.2335137457229237 2214 1 -0.16286705659362966 2214 2 -0.3626404824433713 2214 3 -0.20796040218023037 2214 4 -0.15511185739715216 2214 5 -0.12617152826540945 2214 6 -0.18728660039742095 2214 7 -0.008805219542374232 2214 8 -0.05646966781663565 2214 9 -0.4260518766471364 2214 10 -0.07223327167731952 2214 11 -0.3235112143323917 2214 12 -0.1637377848724526 2214 13 -0.2479982379864225 2214 14 -0.26648263892307256 2214 15 -0.2718296266858786 2214 16 -0.40947146032435455 2214 17 -0.3308284396500838 2214 18 -0.13009429857306998 2214 19 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-0.7584776805814641 2216 6 -0.6287130325786023 2216 7 -0.49511807133432484 2216 8 -0.53812028236392 2216 9 -0.3937961529629703 2216 10 -0.47666481400579386 2216 11 -0.4784661603914344 2216 12 -0.43400172456568953 2216 13 -1.0072274698752324 2216 14 -0.8102261473274979 2216 15 -1.2745823043291153 2216 16 -0.9498872760844037 2216 17 -2.0009111715408423 2216 18 -0.9431132674085845 2216 19 -0.48727880510554655 2216 20 -0.5779783975750661 2216 21 -0.4121382682470909 2216 22 -0.35663641076681785 2216 23 -0.4087601893308559 2216 24 -0.5507195274294858 2216 25 -0.40327152303370895 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3151.0 3 338.0 4 406.0 5 325.0 6 238.0 7 178.0 8 320.0 9 699.0 10 1290.0 11 2365.0 12 4158.0 13 6990.0 14 12322.0 15 21977.0 16 30189.0 17 31908.0 18 29828.0 19 28132.0 20 27428.0 21 28288.0 22 28257.0 23 26255.0 24 25939.0 25 25413.0 26 24687.0 27 25718.0 28 26856.0 29 29812.0 30 35048.0 31 43997.0 32 57554.0 33 80132.0 34 115117.0 35 182323.0 36 336386.0 37 1092308.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.995394920751025 17.45650816870858 9.996279785362532 23.551817125177866 2 9.973178215622966 27.1068366793044 31.851215292573233 31.068769812499397 3 23.634564222802975 34.45051696685903 23.81293290515892 18.101985905179074 4 13.289378803131498 24.663841918241886 25.062103727819572 36.98467555080705 5 9.118676763289205 45.749991808326605 32.89246727321457 12.238864155169619 6 31.8806746230354 28.166410262439246 19.80794035746556 20.144974757059796 7 21.014086281095835 37.9883279101501 21.374451558727824 19.62313425002624 8 29.78023422476826 35.272761785255184 21.31569697343837 13.631307016538182 9 28.889214587392026 21.468734133571758 16.544172766164387 33.09787851287183 10 18.09879323458742 36.92287209580204 27.77374817985605 17.204586489754483 11 23.79196641553055 33.20757632442705 13.879584829715771 29.120872430326628 12 25.249292758951842 38.80971866986194 23.362910713153447 12.578077858032778 13 30.30764869546958 24.399769016746287 17.59664024358234 27.695942044201793 14 21.23991502438283 35.04055826242583 21.51324290151672 22.206283811674616 15 39.33728330410254 21.84751478089923 18.038764354194058 20.776437560804176 16 14.755113001028331 30.167955265676184 35.882944010292555 19.19398772300293 17 29.229327373051838 33.316803433740226 24.31024759998035 13.143621593227584 18 29.593831318663554 24.50537466895155 27.728412407739995 18.1723816046449 19 23.044994293517284 36.688316868933974 28.571338592199375 11.695350245349363 20 24.546991722718413 29.448991614386927 24.98275303905518 21.021263623839477 21 16.35289736076045 33.27687347294902 31.49679232694542 18.873436839345104 22 21.017243080618172 32.768193623020444 15.016365495363285 31.198197800998095 23 13.62751495514925 34.41191809709531 25.8773001704001 26.083266777355345 24 19.806615784630647 44.23310093013079 18.270789139136596 17.68949414610197 25 20.840756017728772 26.5972314962032 15.849184491608062 36.71282799445997 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 23358.0 1 23358.0 2 25617.5 3 27877.0 4 27877.0 5 27877.0 6 33001.5 7 38126.0 8 38126.0 9 38126.0 10 35804.0 11 33482.0 12 33482.0 13 33482.0 14 31982.5 15 30483.0 16 30483.0 17 30483.0 18 30927.5 19 31372.0 20 31372.0 21 31372.0 22 33766.0 23 36160.0 24 36160.0 25 36160.0 26 40923.5 27 45687.0 28 45687.0 29 45687.0 30 48583.0 31 51479.0 32 51479.0 33 51479.0 34 55114.0 35 58749.0 36 58749.0 37 58749.0 38 64554.0 39 70359.0 40 70359.0 41 70359.0 42 115206.5 43 160054.0 44 160054.0 45 160054.0 46 539902.5 47 919751.0 48 919751.0 49 919751.0 50 755044.0 51 590337.0 52 590337.0 53 590337.0 54 378373.5 55 166410.0 56 166410.0 57 166410.0 58 105058.5 59 43707.0 60 43707.0 61 43707.0 62 35326.0 63 26945.0 64 26945.0 65 26945.0 66 21286.5 67 15628.0 68 15628.0 69 15628.0 70 12061.5 71 8495.0 72 8495.0 73 8495.0 74 6334.0 75 4173.0 76 4173.0 77 4173.0 78 3020.0 79 1867.0 80 1867.0 81 1867.0 82 1458.5 83 1050.0 84 1050.0 85 1050.0 86 701.5 87 353.0 88 353.0 89 353.0 90 321.5 91 290.0 92 290.0 93 290.0 94 190.0 95 90.0 96 90.0 97 90.0 98 70.0 99 50.0 100 50.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3116498458722424 2 0.18073763415987382 3 0.19896644725042448 4 0.21937433684835134 5 0.24581659215901225 6 0.19594926439405752 7 0.19201016455380057 8 0.21434569875440637 9 0.21476475192890176 10 0.2237324898631037 11 0.1992597844725713 12 0.20512652891550714 13 0.2182009879597642 14 0.20713798415308515 15 0.19741595050479147 16 0.2013550503450484 17 0.2006845652658557 18 0.20462366510611263 19 0.20240268328128694 20 0.19917597383767222 21 0.19691308669539695 22 0.20101980780545206 23 0.20441413851886495 24 0.2207991176416358 25 0.214471414706755 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2386332.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 16.900696522257967 #Duplication Level Percentage of deduplicated Percentage of total 1 78.38593270846152 13.247768603198429 2 10.368220826926773 3.5046030734328792 3 4.119257644859752 2.0885497005829734 4 1.9774114580785829 1.3367852381048704 5 1.1523386588905224 0.9737662982387233 6 0.7269603327978384 0.7371681581001553 7 0.548237608788107 0.6485918213751329 8 0.36547501429597923 0.49414258424673885 9 0.2827021503821068 0.4300076924817949 >10 1.7365268587945677 5.596469501536277 >50 0.16374084430748562 1.9308228538730574 >100 0.12715962864657887 4.309738119992266 >500 0.015926059163756105 1.896579168093572 >1k 0.01891219525696037 6.996600664413314 >5k 0.00273729141877058 3.0544782645977455 >10k+ 0.00846071893074543 52.75392825773205 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 198642 8.324156068811883 No Hit CCCATGTACTCTGCGTTGATACCAC 188871 7.91469921201241 No Hit GAGTACATGGGAAGCAGTGGTATCA 93628 3.9235110621657 No Hit CATGTACTCTGCGTTGATACCACTG 71043 2.9770794675677985 No Hit GCGTTGATACCACTGCTTCCCATGT 59486 2.492779713803444 No Hit ACGCAGAGTACATGGGAAGCAGTGG 49242 2.063501641850338 No Hit TATCAACGCAGAGTACATGGGAAGC 48908 2.049505265822191 No Hit GTATCAACGCAGAGTACATGGGAAG 48652 2.038777504555108 No Hit ACTCTGCGTTGATACCACTGCTTCC 43386 1.818104102865821 No Hit GGTATCAACGCAGAGTACATGGGAA 41861 1.7541984937552695 No Hit GCTTCCCATGTACTCTGCGTTGATA 36241 1.5186906096888446 No Hit GTGGTATCAACGCAGAGTACATGGG 21347 0.8945528115953689 No Hit GTACTCTGCGTTGATACCACTGCTT 21223 0.8893565522316257 No Hit ATACCACTGCTTCCCATGTACTCTG 20737 0.8689905679511485 No Hit CAGTGGTATCAACGCAGAGTACATG 20507 0.8593523449377539 No Hit GGGAAGCAGTGGTATCAACGCAGAG 19047 0.7981705814614227 No Hit ACATGGGAAGCAGTGGTATCAACGC 18376 0.7700521134527802 No Hit GCAGTGGTATCAACGCAGAGTACAT 17943 0.7519071109971287 No Hit CATGGGAAGCAGTGGTATCAACGCA 17787 0.745369881475 No Hit TACCACTGCTTCCCATGTACTCTGC 17454 0.7314154107643027 No Hit CTGCTTCCCATGTACTCTGCGTTGA 17421 0.7300325352884678 No Hit GTTGATACCACTGCTTCCCATGTAC 17005 0.7125999232294584 No Hit GATACCACTGCTTCCCATGTACTCT 16887 0.7076550957704125 No Hit GCAGAGTACATGGGAAGCAGTGGTA 16226 0.6799556809362653 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 15910 0.6667136006222102 No Hit CCATGTACTCTGCGTTGATACCACT 15395 0.6451323621356961 No Hit CTTCCCATGTACTCTGCGTTGATAC 15205 0.6371703518202831 No Hit CCACTGCTTCCCATGTACTCTGCGT 14539 0.6092614103988883 No Hit AAGCAGTGGTATCAACGCAGAGTAC 14270 0.5979888800049616 No Hit ATGGGAAGCAGTGGTATCAACGCAG 12302 0.5155192152642634 No Hit TTCCCATGTACTCTGCGTTGATACC 12301 0.5154773099468137 No Hit GTACATGGTAAGCAGTGGTATCAAC 12045 0.5047495486797311 No Hit TGATACCACTGCTTCCCATGTACTC 10354 0.4338876568725559 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 10118 0.423998001954464 No Hit ACCATGTACTCTGCGTTGATACCAC 9192 0.3851936779961883 No Hit ATCAACGCAGAGTACATGGGAAGCA 9015 0.3777764368076194 No Hit CCCCATGTACTCTGCGTTGATACCA 7407 0.31039268634875616 No Hit TCCATGTACTCTGCGTTGATACCAC 6848 0.2869676138944623 No Hit GTATCAACGCAGAGTACTTTTTTTT 6202 0.25989677882205825 No Hit AAAAAGTACTCTGCGTTGATACCAC 6085 0.25499385668046193 No Hit GGAAGCAGTGGTATCAACGCAGAGT 5816 0.24372132628653514 No Hit GTACATGGGTAAGCAGTGGTATCAA 5733 0.24024318493822316 No Hit GGTAAGCAGTGGTATCAACGCAGAG 5665 0.23739362335165434 No Hit GAACAAAAAAAAAAAAAAAAAAAAA 5379 0.2254087025610854 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 5286 0.221511508038278 No Hit TATCAACGCAGAGTACTTTTTTTTT 4749 0.19900835256787403 No Hit GGTATCAACGCAGAGTACTTTTTTT 4703 0.1970807079651951 No Hit CTGCGTTGATACCACTGCTTCCCAT 4606 0.19301589217258958 No Hit GAGTACATGGTAAGCAGTGGTATCA 4463 0.18702343177730507 No Hit TCCCATGTACTCTGCGTTGATACCA 4396 0.18421577550818577 No Hit CTCTGCGTTGATACCACTGCTTCCC 4353 0.1824138468578555 No Hit TGGGAAGCAGTGGTATCAACGCAGA 4283 0.17948047463638755 No Hit GTACATGGAAAGCAGTGGTATCAAC 4281 0.1793966640014885 No Hit AAAGTACTCTGCGTTGATACCACTG 4149 0.17386516209814898 No Hit TCAACGCAGAGTACATGGGAAGCAG 3843 0.16104213495858916 No Hit GCTTACCATGTACTCTGCGTTGATA 3764 0.15773161488007537 No Hit GTACATGGAAGCAGTGGTATCAACG 3732 0.15639064472169004 No Hit AGTGGTATCAACGCAGAGTACATGG 3407 0.14277141655058895 No Hit TTGATACCACTGCTTCCCATGTACT 3071 0.1286912298875429 No Hit GAGTACATGGAAAGCAGTGGTATCA 3070 0.12864932457009334 No Hit ACTCTGCGTTGATACCACTGCTTAC 3067 0.12852360861774473 No Hit GCGTTGATACCACTGCTTACCATGT 2977 0.12475213004728596 No Hit NNNNNNNNNNNNNNNNNNNNNNNNN 2838 0.11892729092179966 No Hit AACGCAGAGTACATGGGAAGCAGTG 2768 0.11599391870033171 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2699 0.11310245179631333 No Hit ACTGCTTCCCATGTACTCTGCGTTG 2669 0.11184529227282708 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 2644 0.11079765933658854 No Hit GCTTTCCATGTACTCTGCGTTGATA 2641 0.11067194338423993 No Hit ACGCAGAGTACATGGTAAGCAGTGG 2524 0.10576902124264352 No Hit ATGGTAAGCAGTGGTATCAACGCAG 2474 0.10367375537016645 No Hit CTGCTTACCATGTACTCTGCGTTGA 2420 0.10141086822789117 No Hit ATGTACTCTGCGTTGATACCACTGC 2407 0.10086609910104713 No Hit CGTTGATACCACTGCTTCCCATGTA 2387 0.10002799275205629 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 4.1905317449541805E-5 0.0 2 0.0 0.0 0.0 4.1905317449541805E-5 0.0 3 0.0 0.0 0.0 8.381063489908361E-5 0.0 4 0.0 0.0 0.0 8.381063489908361E-5 0.0 5 0.0 0.0 0.0 8.381063489908361E-5 0.0 6 0.0 0.0 0.0 8.381063489908361E-5 0.0 7 0.0 0.0 0.0 8.381063489908361E-5 0.0 8 0.0 0.0 0.0 1.2571595234862543E-4 0.0 9 0.0 0.0 0.0 1.6762126979816722E-4 0.0 10 0.0 0.0 0.0 4.609584919449599E-4 0.0 11 0.0 0.0 0.0 5.028638093945017E-4 0.0 12 0.0 0.0 0.0 7.962010315412944E-4 0.0 13 0.0 0.0 0.0 8.381063489908362E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGCCGA 25 0.006035351 18.998718 7 GTACAAA 975 0.0 16.288483 1 TACATAA 805 0.0 14.993808 2 GTACATA 1150 0.0 14.967509 1 GGTGTAA 40 0.0052444064 14.264615 1 TTTAAGG 40 0.005265654 14.255925 2 AACTATA 40 0.0052825566 14.24904 7 GCGGTTC 40 0.00528992 14.246048 11 GCGAATG 40 0.005291394 14.245449 14 TACAAAA 1030 0.0 14.025246 2 CCAGTAC 55 1.9539396E-4 13.823928 3 TTAAGCT 50 0.001493373 13.307766 4 GCTTAGG 50 0.0014952658 13.30553 2 AGCCCTA 50 0.0015035707 13.2957535 13 AGAACAA 1450 0.0 12.977807 2 GTAAAAA 450 0.0 12.8909855 1 CTACACT 60 4.0733392E-4 12.674063 4 ACTCTGG 75 1.4803918E-5 12.6658125 8 ATGTACG 115 5.2823452E-9 12.39854 4 CGTTTAT 70 1.0946289E-4 12.210386 14 >>END_MODULE