FastQCFastQC Report
Wed 25 May 2016
SRR1295003_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295003_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2386332
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC2119958.883717772715617No Hit
GTACATGGGAAGCAGTGGTATCAAC1950758.174679801469368No Hit
GAGTACATGGGAAGCAGTGGTATCA1015494.255443081683521No Hit
CATGTACTCTGCGTTGATACCACTG802143.3613931338975465No Hit
GCGTTGATACCACTGCTTCCCATGT646092.7074606550974467No Hit
ACGCAGAGTACATGGGAAGCAGTGG610632.5588643994213713No Hit
TATCAACGCAGAGTACATGGGAAGC538412.2562241968007806No Hit
GTATCAACGCAGAGTACATGGGAAG505872.119864293819971No Hit
ACTCTGCGTTGATACCACTGCTTCC502602.106161255013971No Hit
GGTATCAACGCAGAGTACATGGGAA453131.8988556495910878No Hit
GCTTCCCATGTACTCTGCGTTGATA405061.6974167886111404No Hit
CAGTGGTATCAACGCAGAGTACATG248101.0396709259231323No Hit
ACATGGGAAGCAGTGGTATCAACGC234110.9810453868112232No Hit
CATGGGAAGCAGTGGTATCAACGCA226430.9488621030099752No Hit
GGGAAGCAGTGGTATCAACGCAGAG223060.9347400110294797No Hit
ATACCACTGCTTCCCATGTACTCTG221120.9266103794442685No Hit
GTGGTATCAACGCAGAGTACATGGG220400.9235931965879015No Hit
GCAGTGGTATCAACGCAGAGTACAT212260.8894822681839745No Hit
GTACTCTGCGTTGATACCACTGCTT207280.8686134200941026No Hit
TACCACTGCTTCCCATGTACTCTGC189900.795781978366799No Hit
CTGCTTCCCATGTACTCTGCGTTGA187010.7836713416238813No Hit
GATACCACTGCTTCCCATGTACTCT185290.7764636270225601No Hit
GCAGAGTACATGGGAAGCAGTGGTA182030.7628024935340095No Hit
CCATGTACTCTGCGTTGATACCACT181200.7593243521856976No Hit
GTACTTTTTTTTTTTTTTTTTTTTT172700.7237048323535871No Hit
GTATCAACGCAGAGTACTTTTTTTT172060.7210228920368164No Hit
CTTCCCATGTACTCTGCGTTGATAC171660.7193466793388347No Hit
CCACTGCTTCCCATGTACTCTGCGT166800.6989806950583574No Hit
GTTGATACCACTGCTTCCCATGTAC166160.6962987547415866No Hit
AAGCAGTGGTATCAACGCAGAGTAC165830.6949158792657518No Hit
TATCAACGCAGAGTACTTTTTTTTT152040.6371284465028336No Hit
ATGGGAAGCAGTGGTATCAACGCAG145440.6094709369861361No Hit
GGTATCAACGCAGAGTACTTTTTTT144770.6066632807170167No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA144490.6054899318284296No Hit
GTACATGGTAAGCAGTGGTATCAAC123120.5159382684387587No Hit
TTCCCATGTACTCTGCGTTGATACC120190.503660010426043No Hit
ACCATGTACTCTGCGTTGATACCAC105270.44113727679132664No Hit
TGATACCACTGCTTCCCATGTACTC104440.43765913544301466No Hit
ATCAACGCAGAGTACATGGGAAGCA104300.43707246099872105No Hit
ACGCAGAGTACTTTTTTTTTTTTTT104250.4368629344114733No Hit
GAGTACTTTTTTTTTTTTTTTTTTT93220.3906413692646288No Hit
CCCCATGTACTCTGCGTTGATACCA86390.36202003744659167No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA73760.3090936215078204No Hit
TCCATGTACTCTGCGTTGATACCAC71290.29874300809778354No Hit
GGAAGCAGTGGTATCAACGCAGAGT70030.29346293809914126No Hit
GGTAAGCAGTGGTATCAACGCAGAG65150.27301314318376485No Hit
GTACATGGGTAAGCAGTGGTATCAA62850.2633749201703703No Hit
AAAAAGTACTCTGCGTTGATACCAC62670.26262062445627854No Hit
TGGGAAGCAGTGGTATCAACGCAGA51260.21480665724635128No Hit
GAGTACATGGTAAGCAGTGGTATCA50500.2116218531201861No Hit
CTGCGTTGATACCACTGCTTCCCAT49570.20772465859737874No Hit
CTCTGCGTTGATACCACTGCTTCCC49150.20596463526449796No Hit
TCCCATGTACTCTGCGTTGATACCA47080.19729023455244285No Hit
TCAACGCAGAGTACATGGGAAGCAG45740.19167492201420422No Hit
GCTTACCATGTACTCTGCGTTGATA44010.1844253020954335No Hit
AAAGTACTCTGCGTTGATACCACTG43530.1824138468578555No Hit
GTACATGGAAAGCAGTGGTATCAAC42770.17922904273169032No Hit
GTACATGGAAGCAGTGGTATCAACG39040.1635983593230112No Hit
AGTGGTATCAACGCAGAGTACATGG38230.16020402860959834No Hit
ACTCTGCGTTGATACCACTGCTTAC35470.14863816099352478No Hit
GAGTACATGGAAAGCAGTGGTATCA35070.14696194829554313No Hit
AACGCAGAGTACATGGGAAGCAGTG33330.13967042305932284No Hit
ACGCAGAGTACATGGTAAGCAGTGG31780.13317509885464385No Hit
GCAGAGTACTTTTTTTTTTTTTTTT31720.13292366694994662No Hit
GCGTTGATACCACTGCTTACCATGT31490.13195984464860716No Hit
ACTGCTTCCCATGTACTCTGCGTTG29400.12320163330165292No Hit
GAACAAAAAAAAAAAAAAAAAAAAA28790.12064540893723086No Hit
GCTTTCCATGTACTCTGCGTTGATA27770.11637106655737761No Hit
CAACGCAGAGTACATGGGAAGCAGT27110.11360531560570784No Hit
TTGATACCACTGCTTCCCATGTACT26940.11289292520906563No Hit
CGCAGAGTACATGGGAAGCAGTGGT26940.11289292520906563No Hit
CGTTGATACCACTGCTTCCCATGTA26900.11272530393926745No Hit
GTGGTATCAACGCAGAGTACTTTTT26630.11159386036812982No Hit
ATGGTAAGCAGTGGTATCAACGCAG26270.11008526893994633No Hit
CTGCTTACCATGTACTCTGCGTTGA26220.10987574235269862No Hit
TATCAACGCAGAGTACATGGTAAGC25510.10690046481378115No Hit
GTATCAACGCAGAGTACATGGTAAG24890.10430233513190956No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTAA250.00598936119.02391
GGACCGT250.006035232518.9987856
ATACGAC402.763685E-416.62358919
TAAATAC350.00217128116.2850153
GTACAAA5400.015.67711
GGTATCG1151.8189894E-1214.86830217
GAACAAA9900.014.7002851
TGGGACG1700.014.5284826
CGCAAAA4000.014.4874852
TACAAAA5800.014.4137772
AGGTGTC400.00528244214.2490887
GTATCGA1203.6379788E-1214.24879118
TGATACG551.9622578E-413.81700817
TACATAA1950.013.6410112
AAAAAGT10650.013.3971111
AAAAGTA10900.013.3348472
AGATCCG500.001500443913.2994295
ACAGTCC500.001500680813.299158
GATACGA500.001500917813.29887118
GGACGCA1650.013.2415778
Produced by FastQC (version 0.11.5)