FastQCFastQC Report
Wed 25 May 2016
SRR1295002_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295002_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4730432
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC218400.461691448053793No Hit
CCCATGTACTCTGCGTTGATACCAC194400.4109561240918377No Hit
GAGTACATGGGAAGCAGTGGTATCA103940.2197262321919013No Hit
GTATCAACGCAGAGTACTTTTTTTT100530.21251758824564013No Hit
CATGTACTCTGCGTTGATACCACTG72560.15338979611164477No Hit
GTATCAACGCAGAGTACATGGGAAG70740.14954236737786317No Hit
GCGTTGATACCACTGCTTCCCATGT68930.14571607836239905No Hit
GGTATCAACGCAGAGTACTTTTTTT68780.1453989825876368No Hit
ACGCAGAGTACATGGGAAGCAGTGG60220.12730338370787278No Hit
TATCAACGCAGAGTACTTTTTTTTT57300.12113058595916822No Hit
GCTTCCCATGTACTCTGCGTTGATA56910.12030613694478642No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN56530.11950282764872215No Hit
ATTCCATTCCATTCCATTCCATTCC54010.11417561863271684No Hit
GGTATCAACGCAGAGTACATGGGAA52490.11096238144845968No Hit
TATCAACGCAGAGTACATGGGAAGC47520.10045594144467143No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACCGT701.09270084E-412.2132176
AACCGTC802.8705883E-511.8744637
TACCGTT802.8705883E-511.8744637
GTACCGT907.4767486E-611.6100946
AGTACCG700.001486880510.8619465
CCGGATT700.001495844710.85412317
CGCAGAA1606.151822E-910.09693052
CGCTCTG2350.09.7064195
GTCTAGG4700.09.5099491
AGAACCG1002.745288E-49.5042035
AGGACCG800.0045018739.5042025
ATACCGT1401.0612221E-69.4991686
TAGGACC3050.09.3489914
TAGACTG6000.09.1873965
TTACGGA1151.1054559E-49.0915564
GTCTCGC2750.08.9912251
TGTACCG850.00741249848.9451325
ACCGTCC850.00744154968.9408678
GTCCTAA4000.08.7967021
GTATAGG4800.08.7174531
Produced by FastQC (version 0.11.5)