FastQCFastQC Report
Wed 25 May 2016
SRR1295002_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295002_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4730432
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT234080.49483852637560377No Hit
GTACATGGGAAGCAGTGGTATCAAC217590.45997913087007697No Hit
CCCATGTACTCTGCGTTGATACCAC211890.44792949142911265No Hit
GGTATCAACGCAGAGTACTTTTTTT172810.3653154722443955No Hit
TATCAACGCAGAGTACTTTTTTTTT145150.306843011378242No Hit
GAGTACATGGGAAGCAGTGGTATCA116610.24651025530015017No Hit
ACGCAGAGTACTTTTTTTTTTTTTT90900.1921600395059056No Hit
CATGTACTCTGCGTTGATACCACTG83970.17751021471189102No Hit
ACGCAGAGTACATGGGAAGCAGTGG75400.15939347611380947No Hit
GTATCAACGCAGAGTACATGGGAAG74850.158230791606348No Hit
GCGTTGATACCACTGCTTCCCATGT72510.15328409752005737No Hit
GCTTCCCATGTACTCTGCGTTGATA63430.1340892332877843No Hit
GGTATCAACGCAGAGTACATGGGAA57500.12155338032551782No Hit
TATCAACGCAGAGTACATGGGAAGC54970.11620503159119504No Hit
ACTCTGCGTTGATACCACTGCTTCC53610.11333002990001759No Hit
ATTCCATTCCATTCCATTCCATTCC52200.11034932961725272No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCGG400.005283104714.2492315
GGCCGGT701.09257555E-412.2133686
GGCGCAA1303.2761818E-810.960615
CCGCTTG700.001493445710.8562139
GGACCGT750.002650933310.1325726
TGGACGG856.601525E-410.0582815
GCGCAAT1252.5759673E-69.87915316
TAGACTG7300.09.6296185
TATACTG9000.09.4994875
CGCTTGG800.00452152369.499186510
GTCTAAG3800.09.2622291
CGCAATC1356.862285E-69.14736517
GACTGCG1151.1124756E-49.0862737
TACACCG950.00182578858.9995165
TTACACT7850.08.954934
GTCTTAG5100.08.9529961
AGGACCG850.00744273758.9406945
AGGCGTC850.00744402838.9405057
ACTCCGC850.00744402838.9405058
TCTAGAC4700.08.8931373
Produced by FastQC (version 0.11.5)