FastQCFastQC Report
Wed 25 May 2016
SRR1295001_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295001_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3852595
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC317300.8236007158811139No Hit
CCCATGTACTCTGCGTTGATACCAC284950.7396313393959136No Hit
GAGTACATGGGAAGCAGTGGTATCA156190.40541505141339795No Hit
CATGTACTCTGCGTTGATACCACTG111790.2901680555573581No Hit
GCGTTGATACCACTGCTTCCCATGT93500.24269356109323714No Hit
GCTTCCCATGTACTCTGCGTTGATA87660.22753494722388418No Hit
GTATCAACGCAGAGTACATGGGAAG83460.21663320437263714No Hit
ACGCAGAGTACATGGGAAGCAGTGG83270.2161400302912712No Hit
TATCAACGCAGAGTACATGGGAAGC82200.21336268151726304No Hit
GGTATCAACGCAGAGTACATGGGAA76350.19817811111731182No Hit
ACTCTGCGTTGATACCACTGCTTCC69300.17987875704557577No Hit
GTATCAACGCAGAGTACTTTTTTTT67060.17406449419157735No Hit
GGTATCAACGCAGAGTACTTTTTTT58570.15202739971369947No Hit
TATCAACGCAGAGTACTTTTTTTTT47260.12267056360712714No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN45790.11885495360919068No Hit
GTGGTATCAACGCAGAGTACATGGG44120.11452021299929009No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA39620.10283977423009687No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGAC550.003056111312.0981583
GCCCCGA752.081847E-411.396152511
TACCGTG1053.4753284E-610.8557397
TCCGTAC909.464846E-510.5618843
TAGACTG5600.09.8458255
GTCTAGG4100.09.7435041
CGCAAAA1606.5831046E-89.5056952
GGTCTAC1651.0473195E-79.2232881
CGGGTTC2252.6375346E-108.86402118
GTCTTAG3550.08.841691
GTATTAG5200.08.779861
GTGCTAG4700.08.7020241
GTCTAAG3950.08.6687221
GCACCGT1107.159425E-48.6350216
ATACCGT1651.0534077E-68.6350216
GCTAGAC2002.5158442E-88.5551263
ATCCGTA1000.00291531048.54711511
CTAGGAC4050.08.4495073
TAGACAG5550.08.3929625
TCTACAC4200.08.3740653
Produced by FastQC (version 0.11.5)