FastQCFastQC Report
Wed 25 May 2016
SRR1295001_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295001_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3852595
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC317830.8249764120028189No Hit
CCCATGTACTCTGCGTTGATACCAC311530.8086237977259483No Hit
GAGTACATGGGAAGCAGTGGTATCA178330.46288281015782873No Hit
GTATCAACGCAGAGTACTTTTTTTT171160.44427197771891414No Hit
GGTATCAACGCAGAGTACTTTTTTT156420.40601205161715676No Hit
TATCAACGCAGAGTACTTTTTTTTT129010.3348652012474708No Hit
CATGTACTCTGCGTTGATACCACTG127530.3310236347189362No Hit
ACGCAGAGTACATGGGAAGCAGTGG107630.2793701388285039No Hit
GCGTTGATACCACTGCTTCCCATGT100770.26156395883813377No Hit
TATCAACGCAGAGTACATGGGAAGC93090.24162934333871064No Hit
GCTTCCCATGTACTCTGCGTTGATA92020.23885199456470252No Hit
GTATCAACGCAGAGTACATGGGAAG89300.23179181824199013No Hit
GGTATCAACGCAGAGTACATGGGAA86280.22395294600133156No Hit
ACTCTGCGTTGATACCACTGCTTCC76370.19823002417850827No Hit
ACGCAGAGTACTTTTTTTTTTTTTT71650.18597854173615447No Hit
GTGGTATCAACGCAGAGTACATGGG50280.1305094358477857No Hit
ACATGGGAAGCAGTGGTATCAACGC46820.12152847626080603No Hit
CAGTGGTATCAACGCAGAGTACATG43420.1127032558574156No Hit
GTACTTTTTTTTTTTTTTTTTTTTT42750.1109641683073357No Hit
CATGGGAAGCAGTGGTATCAACGCA39420.10232064361813271No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG456.769958E-414.7765054
CATACCG604.097442E-412.6655765
ATACCGT1201.2786586E-711.0823796
TCGCGTT700.001492857110.85663110
TACACCG700.001493342110.8562085
TATTCCG1409.714313E-810.1776945
CTAGTAC2450.09.6930433
TTCCGTA1002.7581604E-49.4996757
TCCGTAC1002.7581604E-49.4996758
ACGATTC900.00111531069.49905918
CTGTCGC2750.09.3268339
TTATACT6800.09.2197954
ACTGATC3400.08.9408718
AGCACCG850.00744417648.9404075
CAGGACT4700.08.8928524
GTAGGAC1951.6536433E-88.7684763
CCAGGAC5450.08.7148463
GTCTAGG4050.08.6902211
TCTGTCG2751.8189894E-128.6360688
CTCGGAA1107.1517256E-48.63595712
Produced by FastQC (version 0.11.5)