FastQCFastQC Report
Wed 25 May 2016
SRR1295000_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295000_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4474872
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC483711.080947119828232No Hit
CCCATGTACTCTGCGTTGATACCAC433000.9676254426942268No Hit
GAGTACATGGGAAGCAGTGGTATCA235460.5261826483528467No Hit
CATGTACTCTGCGTTGATACCACTG169900.3796756644659333No Hit
GCGTTGATACCACTGCTTCCCATGT144720.32340589853743307No Hit
GTATCAACGCAGAGTACATGGGAAG128050.286153436344101No Hit
ACGCAGAGTACATGGGAAGCAGTGG124600.2784437186136274No Hit
GCTTCCCATGTACTCTGCGTTGATA123430.27582911868764065No Hit
TATCAACGCAGAGTACATGGGAAGC117950.2635829583505405No Hit
GGTATCAACGCAGAGTACATGGGAA108740.2430013640613631No Hit
ACTCTGCGTTGATACCACTGCTTCC102140.22825233883784832No Hit
GTATCAACGCAGAGTACTTTTTTTT81510.18215046151040745No Hit
GGTATCAACGCAGAGTACTTTTTTT65060.1453896334911926No Hit
GTGGTATCAACGCAGAGTACATGGG61890.13830563198232262No Hit
CAGTGGTATCAACGCAGAGTACATG53310.11913189919175342No Hit
TATCAACGCAGAGTACTTTTTTTTT52870.1181486308435191No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN52050.11631617619453696No Hit
ACATGGGAAGCAGTGGTATCAACGC50080.11191381563539694No Hit
ATACCACTGCTTCCCATGTACTCTG48720.10887462255903632No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA47190.10545553034813063No Hit
GTACTCTGCGTTGATACCACTGCTT47020.10507563121358555No Hit
GGGAAGCAGTGGTATCAACGCAGAG46050.10290797144588716No Hit
CATGGGAAGCAGTGGTATCAACGCA44870.10027102451198604No Hit
GCAGTGGTATCAACGCAGAGTACAT44790.1000922484486707No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACCG704.4211265E-714.9346015
GGACCGT551.9644201E-413.8157146
TATGACG550.003061236612.0955223
CTTACCG550.003072298612.0896989
GACCGTG1158.838797E-710.7381747
TCTACAC5050.09.7859113
TATTCCG1901.0459189E-99.5038385
GTATAGG5300.09.3288041
GTCTAGA4300.09.2870841
ACACCGT1651.07016604E-79.2104776
GTCTTAG4750.09.2079461
GTGTAAG7550.09.1933641
CTGCGCA1554.2722604E-79.1926279
GTAGGGC2501.8189894E-129.1234793
GTGCGCC1054.504682E-49.04580111
ACCGTCT950.00182651538.9990998
TACACTG9600.08.8108495
TTAGACA5200.08.7729694
TAAGACT5850.08.7729694
GCACCGT1752.635952E-78.6841646
Produced by FastQC (version 0.11.5)