##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295000_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4474872
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.09841331774406	33.0	33.0	33.0	27.0	33.0
2	31.068974486868004	33.0	33.0	33.0	27.0	33.0
3	31.103090769970628	33.0	33.0	33.0	27.0	33.0
4	34.537903654003955	37.0	37.0	37.0	27.0	37.0
5	34.885178838634936	37.0	37.0	37.0	33.0	37.0
6	35.02359531177652	37.0	37.0	37.0	33.0	37.0
7	34.96634451220057	37.0	37.0	37.0	33.0	37.0
8	34.8244231343377	37.0	37.0	37.0	33.0	37.0
9	34.78817449973988	37.0	37.0	37.0	33.0	37.0
10	34.71980271167533	37.0	37.0	37.0	27.0	37.0
11	34.89327001979051	37.0	37.0	37.0	33.0	37.0
12	34.81237116949937	37.0	37.0	37.0	33.0	37.0
13	34.713694380532004	37.0	37.0	37.0	27.0	37.0
14	36.16595223282364	40.0	37.0	40.0	27.0	40.0
15	36.29973728857496	40.0	37.0	40.0	27.0	40.0
16	36.38285162123073	40.0	37.0	40.0	33.0	40.0
17	36.317867863036085	40.0	37.0	40.0	27.0	40.0
18	36.251087405405116	40.0	37.0	40.0	27.0	40.0
19	36.33423034223102	40.0	37.0	40.0	27.0	40.0
20	36.34584721082525	40.0	37.0	40.0	27.0	40.0
21	36.343437309491755	40.0	37.0	40.0	27.0	40.0
22	36.268767240716606	40.0	37.0	40.0	27.0	40.0
23	36.18662433249487	40.0	37.0	40.0	27.0	40.0
24	36.24820397097392	40.0	37.0	40.0	27.0	40.0
25	35.179711956006784	37.0	33.0	40.0	27.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.3372332735695025
1101	2	0.2954392095582534
1101	3	0.321849112517274
1101	4	0.30296932856391834
1101	5	0.16668421900893549
1101	6	0.25854347283966206
1101	7	0.3545424296407873
1101	8	0.48173883473862844
1101	9	0.5230607406202594
1101	10	0.6154250181434406
1101	11	0.5589459361506925
1101	12	0.4801945466124735
1101	13	0.5072035090338645
1101	14	0.9676529206752775
1101	15	0.8140982456066368
1101	16	0.8336232146036906
1101	17	0.739998721451002
1101	18	0.7889248030690084
1101	19	0.7174369795842566
1101	20	0.6992052955346537
1101	21	0.7353642104139908
1101	22	0.8586286914106722
1101	23	0.840365279090193
1101	24	0.7576613170632456
1101	25	0.8804575767514962
1102	1	0.2210764775635532
1102	2	0.2715309761683713
1102	3	0.2750682958597075
1102	4	0.3400988823266289
1102	5	0.11738845789014363
1102	6	0.19588136876948425
1102	7	0.23022917135692467
1102	8	0.2713832529154061
1102	9	0.1780566918350388
1102	10	0.5223523128655927
1102	11	0.3082550720059487
1102	12	0.36144687569887424
1102	13	0.3166176082108265
1102	14	0.7012395598075472
1102	15	0.6677230073119702
1102	16	0.5906244248709669
1102	17	0.560581823368004
1102	18	0.6699924519280174
1102	19	0.4955466481236144
1102	20	0.527892005735076
1102	21	0.47433758869631504
1102	22	0.5368195186193674
1102	23	0.6097185662129903
1102	24	0.5770674970605754
1102	25	0.6937466673348283
1103	1	0.2474689580755829
1103	2	0.31296664936085605
1103	3	0.24167972859565623
1103	4	0.2815738092529685
1103	5	0.07939553985008985
1103	6	0.16857789940178236
1103	7	0.23517683394999978
1103	8	0.3622832456582614
1103	9	0.30385975102021945
1103	10	0.5039592058140201
1103	11	0.30825380517267575
1103	12	0.4685477657578332
1103	13	0.4035065805900615
1103	14	0.7177527315255361
1103	15	0.644020556767785
1103	16	0.5677729243341005
1103	17	0.5373368806116758
1103	18	0.5783811775462269
1103	19	0.5019208102265154
1103	20	0.5133673098157843
1103	21	0.5376143724020892
1103	22	0.6319173443896204
1103	23	0.6848485206848096
1103	24	0.5604318044674415
1103	25	0.6712458533944528
1104	1	0.2505405728301717
1104	2	0.19282303704709491
1104	3	0.17475049861386083
1104	4	0.20628908497386078
1104	5	0.1254849093951691
1104	6	0.1520992133125958
1104	7	0.248724338252984
1104	8	0.39854524795437385
1104	9	0.347631976586932
1104	10	0.5073489333205998
1104	11	0.3323660389158718
1104	12	0.4833302069319245
1104	13	0.39233387263254116
1104	14	0.7502137765250367
1104	15	0.6594375304088018
1104	16	0.4376326410658322
1104	17	0.5607029305494962
1104	18	0.5845839306739791
1104	19	0.5340084068944719
1104	20	0.6418600007074957
1104	21	0.544613031160317
1104	22	0.6578582363670833
1104	23	0.659494845243934
1104	24	0.7199750622770651
1104	25	0.755022673329087
1105	1	0.10850269463399087
1105	2	0.14309240771030218
1105	3	0.08271493166206767
1105	4	0.06121705650357967
1105	5	0.10636091175352647
1105	6	0.0982909644193839
1105	7	0.16124980400415012
1105	8	0.1828858676406142
1105	9	0.1892266803128564
1105	10	0.3086277864616491
1105	11	0.17990139342798983
1105	12	0.17706061559358233
1105	13	0.24300463826013896
1105	14	0.5458050202915388
1105	15	0.3743032949285947
1105	16	0.35841004626983164
1105	17	0.28267802968063194
1105	18	0.4030274926540258
1105	19	0.29119885644301746
1105	20	0.3882494119140034
1105	21	0.41912184745074654
1105	22	0.3116194811023618
1105	23	0.5094336725380089
1105	24	0.4043748179404574
1105	25	0.25060319327583613
1106	1	0.0727372259864083
1106	2	-0.13510237391798086
1106	3	-0.09008851916857452
1106	4	-0.0014344206061522868
1106	5	-0.3878165986011197
1106	6	-0.1970604791200543
1106	7	-0.4346071390671611
1106	8	-0.019812482335751724
1106	9	0.27558642502260966
1106	10	-0.1360914592771607
1106	11	-0.24591559675682362
1106	12	-0.5569911747342999
1106	13	-0.10290833853117931
1106	14	-0.1709965402541016
1106	15	-0.05044856711959511
1106	16	-0.0028864396633352385
1106	17	-0.02571506304639115
1106	18	-0.2707200068724305
1106	19	-0.5018934996349742
1106	20	0.007982331402310194
1106	21	0.10910352717070282
1106	22	0.30101152391939934
1106	23	-0.39973556032956736
1106	24	0.1523531931333011
1106	25	0.2460207929857745
1107	1	-1.0738359527998966
1107	2	-1.1760201389520297
1107	3	-0.8413658382857427
1107	4	-1.0244619597080629
1107	5	-0.9704851694406145
1107	6	-0.9160247917676898
1107	7	-1.0148024175937849
1107	8	-0.9440543659895297
1107	9	-0.8878948124778887
1107	10	-0.8161238276137226
1107	11	-0.8220203275633793
1107	12	-0.9308305726128125
1107	13	-0.844148843857063
1107	14	-1.09532485685299
1107	15	-1.001539506052687
1107	16	-0.9933120804552615
1107	17	-1.0856579016250976
1107	18	-1.223922409043972
1107	19	-1.1769497367974964
1107	20	-1.2330583166110998
1107	21	-1.2612679142878491
1107	22	-1.2124766379726424
1107	23	-1.0512216889916886
1107	24	-0.8094104607749699
1107	25	-0.9486622731301537
1108	1	0.01722814888628932
1108	2	-0.17127206897872682
1108	3	0.02879821002359151
1108	4	-0.027310159967655068
1108	5	-0.39871743565128526
1108	6	-0.10539432680425875
1108	7	0.06781192042974027
1108	8	0.19775827469737806
1108	9	-0.12995491508393542
1108	10	-0.42638503299468766
1108	11	-0.04606271841397813
1108	12	0.1639417212374923
1108	13	0.22883808502956526
1108	14	-0.18726706494211243
1108	15	0.20962485735144298
1108	16	-0.6383560064892961
1108	17	-0.5910692221793425
1108	18	-0.4643212984221421
1108	19	0.04685818096040606
1108	20	-0.7692689146324341
1108	21	-0.07204236108285045
1108	22	-0.1021650287967617
1108	23	0.14895586558514395
1108	24	-0.28566097242831745
1108	25	-0.9007267613943526
1109	1	0.13276418547841118
1109	2	0.006299102926668354
1109	3	0.15963024350542554
1109	4	0.20385726251711134
1109	5	0.1599244475862207
1109	6	0.09279436270135477
1109	7	0.14472359038627758
1109	8	0.2555180000410786
1109	9	0.1997396397596205
1109	10	0.2035524424679025
1109	11	0.2043277676281008
1109	12	0.3068908585955512
1109	13	0.22677180358822113
1109	14	0.5434632875111163
1109	15	0.3986568108043187
1109	16	0.3455887198434695
1109	17	0.45770438183281215
1109	18	0.44527762099437496
1109	19	0.36823067951021926
1109	20	0.36022985972030597
1109	21	0.3404656356224507
1109	22	0.3126183024947693
1109	23	0.4275918132697427
1109	24	0.4037594134800955
1109	25	0.42528257835654415
1110	1	0.03518089335859287
1110	2	0.01580952667918112
1110	3	-0.004678149086760897
1110	4	-0.989292118341659
1110	5	-0.2023333481089793
1110	6	-0.016576479954217405
1110	7	0.06454223307382989
1110	8	0.09610436939803435
1110	9	0.12024269246836639
1110	10	-0.09656215650955602
1110	11	-0.004544022094897571
1110	12	0.048031639431435735
1110	13	0.09781553381498753
1110	14	0.23967679784288265
1110	15	0.18475111625001261
1110	16	0.1113475334960583
1110	17	0.2899663964115575
1110	18	0.28931355491516086
1110	19	0.2021486611411092
1110	20	0.13710724835432586
1110	21	0.18304357473564892
1110	22	-0.36910360075493287
1110	23	0.09806005117289374
1110	24	0.23472487467223857
1110	25	0.31815549223613715
1111	1	-0.07982400498895359
1111	2	-0.30087502928533283
1111	3	-0.17171850631460828
1111	4	-0.16352493183213568
1111	5	-0.207596631922371
1111	6	-0.12626649631914688
1111	7	-0.15804272234554873
1111	8	0.03633229648390568
1111	9	-0.07562370669175067
1111	10	-0.06865346045558596
1111	11	-0.12871006910040705
1111	12	0.03552955762889809
1111	13	-0.1345832194650214
1111	14	-0.242197422362878
1111	15	-0.22261693225427592
1111	16	-0.1498837936192956
1111	17	-0.15155573643954057
1111	18	-0.15452719094932377
1111	19	-0.06163082320263413
1111	20	0.030492154347676603
1111	21	-0.1252882618642701
1111	22	-0.07499894474871382
1111	23	-1.549961596367666E-4
1111	24	0.030410715025340096
1111	25	-0.11899040083367396
1112	1	-0.004563847125680809
1112	2	-0.3213543833814221
1112	3	-0.032232948671381934
1112	4	-0.03291157575653614
1112	5	0.06051718489753455
1112	6	-0.015781568872839102
1112	7	-0.13068166003631632
1112	8	0.0646785333854254
1112	9	-0.0645215221363955
1112	10	0.21842498187719173
1112	11	7.44743941829995E-4
1112	12	-0.01605679770008095
1112	13	-0.09850882957815799
1112	14	-0.31080125683094906
1112	15	-0.1478879064068508
1112	16	-0.2681979066091529
1112	17	-0.20294495554475844
1112	18	-0.08883011734003077
1112	19	-0.10844260114421189
1112	20	-0.8699293302628348
1112	21	-1.2647489217810417
1112	22	-1.667285796637323
1112	23	-0.5577968759901921
1112	24	-0.3093031185333217
1112	25	-1.4748565272781207
1113	1	-0.15427103591188995
1113	2	-0.1589654624604826
1113	3	-0.19363653625757138
1113	4	-0.45930483458765536
1113	5	-0.13618056542053836
1113	6	-0.21511364119400866
1113	7	-0.23903952836825226
1113	8	-0.10669540275887357
1113	9	-0.24699388051801918
1113	10	-0.23206191370927343
1113	11	-0.2310569245342009
1113	12	-0.19425172652493217
1113	13	-0.24905694187694394
1113	14	-0.676144295799439
1113	15	-0.5049942735368376
1113	16	-0.5149828340179852
1113	17	-0.49570403609963165
1113	18	-0.970460125564486
1113	19	-1.1018065031203719
1113	20	-1.2447924141190327
1113	21	-1.0655045729251569
1113	22	-0.4921611890973878
1113	23	-2.068835728227299
1113	24	-1.1953364121684942
1113	25	-0.569186419413164
1114	1	-0.2960122995366419
1114	2	-0.3751438021028193
1114	3	-0.31691406229739627
1114	4	-0.5632089856402942
1114	5	-0.6621020619166913
1114	6	-0.47168949555059925
1114	7	-0.4854577650662577
1114	8	-0.2846816493794293
1114	9	-1.1005818771076719
1114	10	-0.36338936098781005
1114	11	-0.4645954985481495
1114	12	-0.3810216262957766
1114	13	-0.3590765820155113
1114	14	-0.7403455739403597
1114	15	-1.2982915876237513
1114	16	-1.195711198963238
1114	17	-1.6527865827885435
1114	18	-1.8828317224799633
1114	19	-1.70252692326509
1114	20	-0.940853610212983
1114	21	-0.7784754106473244
1114	22	-0.5404781486053167
1114	23	-0.3657647247419433
1114	24	-0.95113785977032
1114	25	-0.6014030081989503
1115	1	-0.8213726219422774
1115	2	-0.6433686700062466
1115	3	-0.614990783817813
1115	4	-0.7136814291302969
1115	5	-0.42988913513220695
1115	6	-0.5335804974591483
1115	7	-0.5310352002432879
1115	8	-0.42087322924230364
1115	9	-0.7903649260421801
1115	10	-0.36239979708032166
1115	11	-0.661259151846771
1115	12	-0.7045152309906157
1115	13	-0.6140004224367175
1115	14	-0.644060960369643
1115	15	-1.7745969449222017
1115	16	-1.161410799593213
1115	17	-0.8737486262023495
1115	18	-2.2787720404061105
1115	19	-1.2428696878759808
1115	20	-1.1484883466665678
1115	21	-1.107030083458838
1115	22	-0.8568909040323618
1115	23	-0.5387028732862902
1115	24	-0.599348099369287
1115	25	-0.7185308231407532
1116	1	-1.4053861720607586
1116	2	-0.7191961558160394
1116	3	-1.678112888114125
1116	4	-0.8371469015475768
1116	5	-0.7556073770100653
1116	6	-0.515583951926466
1116	7	-1.265589444831086
1116	8	-2.9831357884783287
1116	9	-1.5248764992161483
1116	10	-0.809969155200001
1116	11	-0.596979514696578
1116	12	-0.7158543968994096
1116	13	-1.6197729864668489
1116	14	-1.6806635935008032
1116	15	-1.4621339377696145
1116	16	-1.1369318874882168
1116	17	-1.7206766057662293
1116	18	-2.450480221105906
1116	19	-1.3644553174438485
1116	20	-1.0343042993262372
1116	21	-0.7877563936483583
1116	22	-0.559029663947392
1116	23	-0.5189975539996752
1116	24	-0.6972115268891557
1116	25	-0.5923512333472445
1201	1	0.2924332366538849
1201	2	0.2817196046392496
1201	3	0.2653090060718668
1201	4	0.4225812499200998
1201	5	0.22938381221110404
1201	6	0.14448341493958594
1201	7	0.16365271326985464
1201	8	0.39273137917168555
1201	9	0.2938550991783444
1201	10	0.5168617745773361
1201	11	0.3547136769036783
1201	12	0.3175280128936606
1201	13	0.31270645284452314
1201	14	0.7697326187158637
1201	15	0.7667415441073118
1201	16	0.6123360374076015
1201	17	0.6693087043662374
1201	18	0.6141717932281239
1201	19	0.521493792846897
1201	20	0.5747636886017418
1201	21	0.597234460735109
1201	22	0.7014173758061943
1201	23	0.7007685854257559
1201	24	0.6718313796277897
1201	25	0.8423786667173374
1202	1	0.31415334626039026
1202	2	0.3178273564950409
1202	3	0.3544153122029847
1202	4	0.4058798900355427
1202	5	0.22957393484430355
1202	6	0.056013563494843766
1202	7	0.1561885413723516
1202	8	0.26506394889541696
1202	9	0.28852554456459245
1202	10	0.4584308301453248
1202	11	0.29184659241749245
1202	12	0.3132870610319287
1202	13	0.3300274056696466
1202	14	0.645198166475538
1202	15	0.48816685927245373
1202	16	0.4916491822828988
1202	17	0.48980573469386
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2210	22	0.6185498987283538
2210	23	0.6462606456819415
2210	24	0.5973680685381808
2210	25	0.6862057348742141
2211	1	0.14239489325205312
2211	2	-0.024715150481597448
2211	3	0.17973617540539166
2211	4	0.08245951310895094
2211	5	0.08558682114271932
2211	6	-0.012727216930905172
2211	7	0.09004916579114308
2211	8	0.1836746268567495
2211	9	0.08447225078005971
2211	10	0.22176262182585305
2211	11	0.07267002834699099
2211	12	0.11176473438354861
2211	13	0.007726642454841226
2211	14	-0.03313009043070281
2211	15	0.005216737289984508
2211	16	-0.10093732946520362
2211	17	0.018464279793221294
2211	18	0.08339472247504176
2211	19	0.05459425255969563
2211	20	0.055268210889771296
2211	21	-0.02821013911589887
2211	22	-0.04413022616889606
2211	23	0.13138639845383437
2211	24	0.16276230073057718
2211	25	0.09006946191718868
2212	1	0.21943146193049756
2212	2	0.1857339325261833
2212	3	0.2447053970982047
2212	4	0.2023900491481072
2212	5	0.291247756327337
2212	6	0.20258838524853928
2212	7	0.15397048854769935
2212	8	0.2941629145148923
2212	9	0.10965332559851504
2212	10	0.39159098098220113
2212	11	0.1744205748116201
2212	12	0.2299005096137634
2212	13	0.19295872886143428
2212	14	0.2800476817849713
2212	15	0.13084253630295706
2212	16	0.0904479514846912
2212	17	0.20404044108866515
2212	18	0.2618857722962602
2212	19	0.12612334588289542
2212	20	0.2220185685420688
2212	21	0.1833919704965794
2212	22	-0.38782593432234336
2212	23	0.21834440032981206
2212	24	0.25646703202596655
2212	25	0.371574468859599
2213	1	-0.02214981092956947
2213	2	-0.7664518262642055
2213	3	-0.10579449322499457
2213	4	-0.06484744301928913
2213	5	0.12061892368347316
2213	6	-0.021371246457931647
2213	7	-0.09327222219286568
2213	8	0.17681837110072962
2213	9	-0.155843256344518
2213	10	-1.454358203274552
2213	11	-0.45730558440258307
2213	12	-0.1430282550548867
2213	13	-0.12807043088772474
2213	14	0.06869778223843781
2213	15	-0.024930607422817275
2213	16	-0.0019517941121236504
2213	17	-0.09474980616270301
2213	18	0.061496882850022416
2213	19	0.0015570889383909048
2213	20	-0.0067601000565744584
2213	21	0.031188695954895707
2213	22	0.09414145510857708
2213	23	-0.8191937707672636
2213	24	-1.3771922226218791
2213	25	-1.2948217791905563
2214	1	-0.038957887626185794
2214	2	-0.2717518676159081
2214	3	-0.07158266933795332
2214	4	-0.15851450597678962
2214	5	-0.08547596359651521
2214	6	-0.2377641723247308
2214	7	-0.146216375375829
2214	8	-0.040584647972998766
2214	9	-0.4168793965943607
2214	10	-0.02416766135061721
2214	11	-0.15043579448822442
2214	12	-0.08685446034774458
2214	13	-0.008959969280390112
2214	14	-0.08718914336961348
2214	15	-0.2118837151980273
2214	16	-0.2740948218042689
2214	17	-0.24847077994597555
2214	18	-0.14519807914184213
2214	19	-0.7031632294510572
2214	20	-0.37141532658921506
2214	21	-0.38765098615645854
2214	22	-0.5499451939749633
2214	23	-1.721457885154038
2214	24	-1.2273457531309901
2214	25	-1.5348187211004287
2215	1	-0.47670385950290495
2215	2	-0.24211511011482045
2215	3	-1.303293535427045
2215	4	-0.9870268859387465
2215	5	-0.41267383480268904
2215	6	-0.3333703672485342
2215	7	-0.2781190517062342
2215	8	-0.2506767276401689
2215	9	-0.48441575498325307
2215	10	-1.41717411871889
2215	11	-0.655578723818671
2215	12	-0.6443447185140201
2215	13	-0.652106019572102
2215	14	-0.9498904797196559
2215	15	-1.0856535940122427
2215	16	-0.7495693155696017
2215	17	-0.6326375564532043
2215	18	-0.4762787559626176
2215	19	-1.0487215320290346
2215	20	-1.3237052648960557
2215	21	-0.7132273244612861
2215	22	-0.48895495479807494
2215	23	-0.6157785720224354
2215	24	-1.732522262954646
2215	25	-0.9160040070917361
2216	1	-0.4282380423698342
2216	2	-0.3316622013462549
2216	3	-1.2560700674270144
2216	4	-0.5530629730122243
2216	5	-0.4641868183059259
2216	6	-0.4943757809737619
2216	7	-0.3202365623252845
2216	8	-0.3836425234631804
2216	9	-0.3027420686795921
2216	10	-0.3036246983249882
2216	11	-0.4572513944347989
2216	12	-0.4019736873061177
2216	13	-1.0768213468504229
2216	14	-0.7256853105620138
2216	15	-1.2264764558931276
2216	16	-0.9061499502276291
2216	17	-1.8033969779928611
2216	18	-0.7970420278436237
2216	19	-0.6473649768592082
2216	20	-0.6769094269455422
2216	21	-0.3996701411150241
2216	22	-0.49805279595008045
2216	23	-0.40819352410522924
2216	24	-0.5240792254511462
2216	25	-0.3875932792450669
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	5783.0
3	603.0
4	702.0
5	706.0
6	415.0
7	313.0
8	556.0
9	1066.0
10	2019.0
11	3833.0
12	6719.0
13	9951.0
14	13978.0
15	18853.0
16	22746.0
17	23621.0
18	23500.0
19	23991.0
20	24483.0
21	26240.0
22	27395.0
23	28761.0
24	30567.0
25	32252.0
26	33885.0
27	38046.0
28	42609.0
29	50623.0
30	61731.0
31	78025.0
32	105283.0
33	150084.0
34	221304.0
35	356432.0
36	659807.0
37	2347990.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.51511878154019	21.167258028325733	13.410127784777682	22.907495405356393
2	14.405230572566808	21.763851872083354	40.01088023569366	23.820037319656176
3	18.53203059137275	27.2007729572444	29.867520233588934	24.399676217793914
4	11.775010873465117	19.186391572851768	38.013634048660265	31.024963505022846
5	12.086837306618756	39.932548980954216	35.34540925362569	12.635204458801338
6	30.18415685473732	38.47677927351533	18.054733649975024	13.284330221772327
7	25.012722891285723	34.0202388567511	23.391447037732522	17.575591214230652
8	24.087064230394635	37.647936431368045	21.353144559650122	16.911854778587195
9	24.070050892152306	17.916433854198743	21.997323823014003	36.01619143063495
10	14.254016517138648	31.572286110131877	34.0211219058361	20.15257546689338
11	30.609578495675326	26.887100862426454	22.976266926764957	19.52705371513326
12	20.535218445906246	29.281295233386363	31.444615525173052	18.738870795534346
13	26.40541184564612	24.423829069813273	26.24127468440157	22.92948440013903
14	19.70746152480177	25.24649722135698	28.254825872674395	26.791215381166854
15	23.81779991092902	30.621340627988484	23.87897183695577	21.681887624126727
16	20.09975469972268	31.97349726990084	27.561674938506993	20.365073091869487
17	20.425192707079642	31.322006952569147	28.62463403847984	19.62816630187137
18	20.531423953488424	30.16592947353595	29.729659526719992	19.572987046255633
19	20.577369155162867	30.49041356805423	29.994471368563563	18.937745908219345
20	21.59008977136649	29.45710848963236	29.00321360533738	19.94958813366377
21	20.86193593226821	29.981666273331953	29.15942505361518	19.99697274078466
22	20.483846914816485	29.76530455503612	28.265917413464255	21.48493111668314
23	19.598883960060817	30.282682347356232	29.042722564204766	21.07571112837818
24	20.326297382257113	30.83496152802152	28.43372150211374	20.405019587607626
25	20.88180921380447	29.0204720106867	27.44951420544569	22.64820457006314
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	12629.0
1	12629.0
2	11701.5
3	10774.0
4	10774.0
5	10774.0
6	14247.0
7	17720.0
8	17720.0
9	17720.0
10	23571.0
11	29422.0
12	29422.0
13	29422.0
14	43600.0
15	57778.0
16	57778.0
17	57778.0
18	84997.0
19	112216.0
20	112216.0
21	112216.0
22	152058.5
23	191901.0
24	191901.0
25	191901.0
26	239869.5
27	287838.0
28	287838.0
29	287838.0
30	334676.0
31	381514.0
32	381514.0
33	381514.0
34	414840.5
35	448167.0
36	448167.0
37	448167.0
38	464046.5
39	479926.0
40	479926.0
41	479926.0
42	479695.0
43	479464.0
44	479464.0
45	479464.0
46	553761.0
47	628058.0
48	628058.0
49	628058.0
50	560829.0
51	493600.0
52	493600.0
53	493600.0
54	413013.5
55	332427.0
56	332427.0
57	332427.0
58	280053.5
59	227680.0
60	227680.0
61	227680.0
62	187423.5
63	147167.0
64	147167.0
65	147167.0
66	113301.0
67	79435.0
68	79435.0
69	79435.0
70	57493.0
71	35551.0
72	35551.0
73	35551.0
74	24912.0
75	14273.0
76	14273.0
77	14273.0
78	9645.0
79	5017.0
80	5017.0
81	5017.0
82	3310.0
83	1603.0
84	1603.0
85	1603.0
86	1052.5
87	502.0
88	502.0
89	502.0
90	330.5
91	159.0
92	159.0
93	159.0
94	92.5
95	26.0
96	26.0
97	26.0
98	25.5
99	25.0
100	25.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3061316614195892
2	0.1800498427664523
3	0.19904479949370618
4	0.22065435614694678
5	0.24340361020382262
6	0.1927429432618408
7	0.19024007837542617
8	0.21285525038481548
9	0.21334688455893264
10	0.22199517662181176
11	0.19817326618504394
12	0.20434104036942285
13	0.216855364801496
14	0.2056595138363734
15	0.19600560641734555
16	0.19926826957285035
17	0.19882132941456201
18	0.20244154469669745
19	0.20177113445926498
20	0.19799449012172862
21	0.1963408115360618
22	0.1992012285491071
23	0.20295552587872903
24	0.21909006559293764
25	0.21245300424235597
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4474872.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.89402928615178
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.93326102090406	63.369156747984555
2	9.804159312927323	14.293293521652439
3	1.8926697198911535	4.138929659722752
4	0.5964712551157862	1.7391677255503133
5	0.23014219751393092	0.8387996042779903
6	0.12380622116245366	0.5414840586734209
7	0.07718270774837242	0.39383109912960473
8	0.052984165580574887	0.3089783454026193
9	0.041724941334961976	0.2737349186070315
>10	0.21799345118778546	2.874106955371555
>50	0.01619944231027967	0.8210144762095226
>100	0.010443095187116247	1.448701013809281
>500	9.566308672483237E-4	0.45967329017226544
>1k	0.0014503753636643164	2.6765192919330336
>5k	2.1601335203500258E-4	0.937499323700859
>10k+	3.394495531978612E-4	4.885109967802757
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	48371	1.080947119828232	No Hit
CCCATGTACTCTGCGTTGATACCAC	43300	0.9676254426942268	No Hit
GAGTACATGGGAAGCAGTGGTATCA	23546	0.5261826483528467	No Hit
CATGTACTCTGCGTTGATACCACTG	16990	0.3796756644659333	No Hit
GCGTTGATACCACTGCTTCCCATGT	14472	0.32340589853743307	No Hit
GTATCAACGCAGAGTACATGGGAAG	12805	0.286153436344101	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	12460	0.2784437186136274	No Hit
GCTTCCCATGTACTCTGCGTTGATA	12343	0.27582911868764065	No Hit
TATCAACGCAGAGTACATGGGAAGC	11795	0.2635829583505405	No Hit
GGTATCAACGCAGAGTACATGGGAA	10874	0.2430013640613631	No Hit
ACTCTGCGTTGATACCACTGCTTCC	10214	0.22825233883784832	No Hit
GTATCAACGCAGAGTACTTTTTTTT	8151	0.18215046151040745	No Hit
GGTATCAACGCAGAGTACTTTTTTT	6506	0.1453896334911926	No Hit
GTGGTATCAACGCAGAGTACATGGG	6189	0.13830563198232262	No Hit
CAGTGGTATCAACGCAGAGTACATG	5331	0.11913189919175342	No Hit
TATCAACGCAGAGTACTTTTTTTTT	5287	0.1181486308435191	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	5205	0.11631617619453696	No Hit
ACATGGGAAGCAGTGGTATCAACGC	5008	0.11191381563539694	No Hit
ATACCACTGCTTCCCATGTACTCTG	4872	0.10887462255903632	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	4719	0.10545553034813063	No Hit
GTACTCTGCGTTGATACCACTGCTT	4702	0.10507563121358555	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	4605	0.10290797144588716	No Hit
CATGGGAAGCAGTGGTATCAACGCA	4487	0.10027102451198604	No Hit
GCAGTGGTATCAACGCAGAGTACAT	4479	0.1000922484486707	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	2.2347007914416323E-5	0.0
8	0.0	0.0	0.0	2.2347007914416323E-5	0.0
9	0.0	0.0	0.0	4.4694015828832646E-5	0.0
10	0.0	0.0	0.0	4.4694015828832646E-5	0.0
11	0.0	0.0	0.0	4.4694015828832646E-5	0.0
12	0.0	0.0	0.0	6.704102374324897E-5	0.0
13	0.0	0.0	0.0	1.1173503957208161E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGACCG	70	4.4211265E-7	14.934601	5
GGACCGT	55	1.9644201E-4	13.815714	6
TATGACG	55	0.0030612366	12.095522	3
CTTACCG	55	0.0030722986	12.089698	9
GACCGTG	115	8.838797E-7	10.738174	7
TCTACAC	505	0.0	9.785911	3
TATTCCG	190	1.0459189E-9	9.503838	5
GTATAGG	530	0.0	9.328804	1
GTCTAGA	430	0.0	9.287084	1
ACACCGT	165	1.07016604E-7	9.210477	6
GTCTTAG	475	0.0	9.207946	1
GTGTAAG	755	0.0	9.193364	1
CTGCGCA	155	4.2722604E-7	9.192627	9
GTAGGGC	250	1.8189894E-12	9.123479	3
GTGCGCC	105	4.504682E-4	9.045801	11
ACCGTCT	95	0.0018265153	8.999099	8
TACACTG	960	0.0	8.810849	5
TTAGACA	520	0.0	8.772969	4
TAAGACT	585	0.0	8.772969	4
GCACCGT	175	2.635952E-7	8.684164	6
>>END_MODULE