FastQCFastQC Report
Wed 25 May 2016
SRR1295000_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295000_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4474872
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC476191.064142169876591No Hit
CCCATGTACTCTGCGTTGATACCAC475571.0627566553858971No Hit
GAGTACATGGGAAGCAGTGGTATCA261330.5839943578274417No Hit
GTATCAACGCAGAGTACTTTTTTTT197040.44032544394565926No Hit
CATGTACTCTGCGTTGATACCACTG191280.42745356738695545No Hit
GGTATCAACGCAGAGTACTTTTTTT169420.37860300808604136No Hit
ACGCAGAGTACATGGGAAGCAGTGG159730.3569487574169719No Hit
GCGTTGATACCACTGCTTCCCATGT154680.34566351842019166No Hit
TATCAACGCAGAGTACTTTTTTTTT141540.31629955002064863No Hit
GCTTCCCATGTACTCTGCGTTGATA137440.30713727677573793No Hit
GTATCAACGCAGAGTACATGGGAAG136020.30396400165189086No Hit
TATCAACGCAGAGTACATGGGAAGC131970.2949134634465522No Hit
GGTATCAACGCAGAGTACATGGGAA120760.2698624675744915No Hit
ACTCTGCGTTGATACCACTGCTTCC116680.26074488834540965No Hit
ACGCAGAGTACTTTTTTTTTTTTTT83100.18570363576879964No Hit
GTGGTATCAACGCAGAGTACATGGG66810.14930035987621545No Hit
CAGTGGTATCAACGCAGAGTACATG65180.1456577975861656No Hit
ACATGGGAAGCAGTGGTATCAACGC63920.14284207458894913No Hit
CATGGGAAGCAGTGGTATCAACGCA58560.13086407834682198No Hit
GCAGTGGTATCAACGCAGAGTACAT54660.12214874526019963No Hit
GGGAAGCAGTGGTATCAACGCAGAG54210.12114312990405089No Hit
GTACTTTTTTTTTTTTTTTTTTTTT53800.12022690257955983No Hit
ATACCACTGCTTCCCATGTACTCTG50480.1128076959519736No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGA508.725505E-515.1988296
GTCTAGG3800.010.5128171
GCACCGT1451.4477337E-810.4819516
GTCTTAG4400.010.37628651
CCGTGCA1106.057029E-610.3629539
AGACCGT1151.0284626E-59.912286
CGCAAAA1356.4562664E-79.8513132
GTCTAGA4400.09.5115971
AGCACCG1401.0606964E-69.499485
CCGTCCA900.00111504679.4993749
GGGCCGG1002.759571E-49.4992696
CTAGGAC4400.09.2835843
GTATTAG6100.09.199741
TAGACAG6750.09.1476485
TATACCG1054.498105E-49.0471255
TAGGCCG950.00182576058.9995085
TGGACTG5200.08.9514345
TTACACC5100.08.5681584
CGTAGCA1000.00290804878.5495332
TAGGACT5350.08.5228994
Produced by FastQC (version 0.11.5)