FastQCFastQC Report
Wed 25 May 2016
SRR1294999_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294999_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5615240
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC487290.8677990611265057No Hit
CCCATGTACTCTGCGTTGATACCAC453660.8079084776429858No Hit
GAGTACATGGGAAGCAGTGGTATCA229590.40886943389774966No Hit
CATGTACTCTGCGTTGATACCACTG172510.3072175009438599No Hit
GCGTTGATACCACTGCTTCCCATGT147020.261823181199735No Hit
GTATCAACGCAGAGTACATGGGAAG130940.23318682727719564No Hit
ACGCAGAGTACATGGGAAGCAGTGG121410.21621515732185978No Hit
TATCAACGCAGAGTACATGGGAAGC115410.20552995063434512No Hit
GCTTCCCATGTACTCTGCGTTGATA109880.19568175180401906No Hit
GGTATCAACGCAGAGTACATGGGAA109740.19543243031464372No Hit
ACTCTGCGTTGATACCACTGCTTCC102790.18305539923493921No Hit
GTATCAACGCAGAGTACTTTTTTTT89640.1596369879114695No Hit
ATTCCATTCCATTCCATTCCATTCC71200.12679778602517436No Hit
GGTATCAACGCAGAGTACTTTTTTT70750.12599639552361075No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN67490.12019076655672777No Hit
GTGGTATCAACGCAGAGTACATGGG62930.11207000947421661No Hit
TATCAACGCAGAGTACTTTTTTTTT58610.10437666065920602No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG752.0646305E-411.4065395
TCGCGTT1354.7493813E-911.25767210
CGCGTTG1354.7602953E-911.25596411
CGCAAAA2200.010.3684692
CTCGCGT1554.0794475E-89.8051589
TAGACAG8500.09.6172775
AGTACCG800.004497239.5054485
ACTCGCG1701.6716513E-89.4987458
GGACGGT900.00111626859.49832256
TAGACTG8350.09.2208555
CTAGACA7050.09.1687854
GAGCCGT850.00745059138.9395976
GAACCGT850.00745059138.9395976
CTAGGAC4750.08.8041813
GTCTTAG5650.08.7536151
CCTAGAC4900.08.7286363
TGGACCG1201.7544086E-48.7133275
CCTACAC6550.08.7064253
TAGGACT6050.08.6417034
ATACCGT1107.1622204E-48.6348386
Produced by FastQC (version 0.11.5)