FastQCFastQC Report
Wed 25 May 2016
SRR1294999_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294999_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5615240
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC506110.9013149927696769No Hit
GTACATGGGAAGCAGTGGTATCAAC478080.8513972688611707No Hit
GAGTACATGGGAAGCAGTGGTATCA249350.4440593812552981No Hit
GTATCAACGCAGAGTACTTTTTTTT217370.38710722961084476No Hit
CATGTACTCTGCGTTGATACCACTG194330.34607603593078834No Hit
GGTATCAACGCAGAGTACTTTTTTT180410.32128635641575426No Hit
GCGTTGATACCACTGCTTCCCATGT158020.2814127267935119No Hit
TATCAACGCAGAGTACTTTTTTTTT153360.27311388293287553No Hit
ACGCAGAGTACATGGGAAGCAGTGG152960.2724015358203746No Hit
GTATCAACGCAGAGTACATGGGAAG136740.24351586040845985No Hit
TATCAACGCAGAGTACATGGGAAGC128240.228378484267814No Hit
GCTTCCCATGTACTCTGCGTTGATA118850.21165613580185352No Hit
GGTATCAACGCAGAGTACATGGGAA118220.21053418909966448No Hit
ACTCTGCGTTGATACCACTGCTTCC116020.2066162799809091No Hit
ACGCAGAGTACTTTTTTTTTTTTTT89400.15920957964396892No Hit
ATTCCATTCCATTCCATTCCATTCC70220.12505253559954693No Hit
GTGGTATCAACGCAGAGTACATGGG66840.11903320249891368No Hit
CAGTGGTATCAACGCAGAGTACATG64510.11488378056859545No Hit
ACATGGGAAGCAGTGGTATCAACGC61860.11016448094827648No Hit
CATGGGAAGCAGTGGTATCAACGCA56750.10106424658607646No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG604.0969285E-412.6659559
ACCGTCC550.00307145712.090238
ACCGATC600.0058813611.082718
TACGGGT951.6466426E-49.9993484
GGCCCGT1151.0285756E-59.9123096
GGACGCA900.00111504179.4994658
GCACCGT1002.7597696E-49.4992976
TAGACAG8850.09.3383745
GTCTAGA6200.09.0507751
ACGTAAG850.00736962268.9515141
CGTGCGC850.0074432378.94067410
GCGGGTT850.0074432378.94067411
CACCGTC850.0074432378.9406747
TAGGACG850.0074437818.9405944
GTCCTAA3750.08.8769181
TATACCG1502.7076294E-68.8660895
CTTAGAC5200.08.768663
TTAGGAC4900.08.7239223
CTGTGCG1554.215539E-68.5801639
CGTTCCA8000.08.5495210
Produced by FastQC (version 0.11.5)