FastQCFastQC Report
Wed 25 May 2016
SRR1294998_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294998_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3963300
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC351080.885827467009815No Hit
CCCATGTACTCTGCGTTGATACCAC312400.7882320288648348No Hit
GAGTACATGGGAAGCAGTGGTATCA165620.41788408649357855No Hit
CATGTACTCTGCGTTGATACCACTG119120.30055761612797416No Hit
GCGTTGATACCACTGCTTCCCATGT109750.27691570156182976No Hit
GCTTCCCATGTACTCTGCGTTGATA98040.24736961622890016No Hit
GTATCAACGCAGAGTACATGGGAAG93160.23505664471526252No Hit
ACGCAGAGTACATGGGAAGCAGTGG89050.2246864986248833No Hit
TATCAACGCAGAGTACATGGGAAGC78830.19889990664345367No Hit
GGTATCAACGCAGAGTACATGGGAA76350.19264249489062146No Hit
ACTCTGCGTTGATACCACTGCTTCC68990.1740721116241516No Hit
GTATCAACGCAGAGTACTTTTTTTT65880.16622511543410795No Hit
GGTATCAACGCAGAGTACTTTTTTT52510.13249060126662127No Hit
GTGGTATCAACGCAGAGTACATGGG50790.12815078343804404No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN46920.11838619332374536No Hit
TATCAACGCAGAGTACTTTTTTTTT41390.10443317437488961No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAC400.00524342114.2653511
TCTACGG602.5598085E-514.2561472
CCGTTCA658.034954E-411.6911419
AAACCGT658.039216E-411.6904026
GTCTACG700.0014789410.868841
GCGCTAT1158.8606794E-710.73602311
ATACCGT1002.4052695E-510.4482966
AGCACCG1106.0142647E-610.3690265
TGAACCG750.00263771710.1386035
AACGGTG750.002650756210.1325787
ACGCTGC951.6467294E-49.9991288
TGGGCCG900.00110902829.5049415
AGGCGCG900.00111507449.4992927
GCACCGT900.00111597739.4984516
TACACTG8450.08.9987615
AGCGTGC850.0074443178.9403978
TAAGGCC2451.6370905E-118.9234574
TAGACTG5900.08.8605385
CTAGGAC3550.08.8349083
GTCTTAC4200.08.8309321
Produced by FastQC (version 0.11.5)