FastQCFastQC Report
Wed 25 May 2016
SRR1294998_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294998_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3963300
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC354450.8943304821739461No Hit
CCCATGTACTCTGCGTTGATACCAC348670.8797466757500063No Hit
GAGTACATGGGAAGCAGTGGTATCA185050.4669088890570989No Hit
GTATCAACGCAGAGTACTTTTTTTT155220.39164332753008857No Hit
GGTATCAACGCAGAGTACTTTTTTT139230.35129816062372266No Hit
CATGTACTCTGCGTTGATACCACTG133900.337849771654934No Hit
ACGCAGAGTACATGGGAAGCAGTGG114730.289480988065501No Hit
TATCAACGCAGAGTACTTTTTTTTT111240.2806751949133298No Hit
GCGTTGATACCACTGCTTCCCATGT107520.2712890772840814No Hit
GCTTCCCATGTACTCTGCGTTGATA102080.2575631418262559No Hit
GTATCAACGCAGAGTACATGGGAAG98330.24810132969999749No Hit
TATCAACGCAGAGTACATGGGAAGC91710.23139807735977594No Hit
GGTATCAACGCAGAGTACATGGGAA85080.21466959352055104No Hit
ACTCTGCGTTGATACCACTGCTTCC80380.2028107889889738No Hit
ACGCAGAGTACTTTTTTTTTTTTTT66250.1671586808972321No Hit
GTGGTATCAACGCAGAGTACATGGG53000.13372694471778568No Hit
CAGTGGTATCAACGCAGAGTACATG50470.12734337546993665No Hit
ACATGGGAAGCAGTGGTATCAACGC49240.12423990109252392No Hit
CATGGGAAGCAGTGGTATCAACGCA42200.10647692579416143No Hit
GCAGTGGTATCAACGCAGAGTACAT40790.10291928443468827No Hit
GGGAAGCAGTGGTATCAACGCAGAG40270.10160724648651377No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCCA550.003071248512.0901959
TCGTGCA752.0765021E-411.3993259
AGACCGC1001.9332492E-611.3993256
CTATCGG1053.4730165E-610.85636414
TATTCCG1303.8495273E-710.2301645
GCTATCG1151.0281479E-59.91245713
ACACCGT1356.455848E-79.851276
CTAGGAC4050.09.851273
TAGACTG6100.09.8108965
TAGGACC2151.0913936E-119.7203564
GTCCTAG3200.09.5103571
ACCGTCT800.0045197899.4995588
CAAGACG800.00452025939.4994394
TACACCG1106.846304E-59.4994385
TTGTGCG1451.7091861E-69.1719887
TCTACAC5500.09.1540043
CGCAAAA1356.856744E-69.1478392
TGCGCTA1356.858938E-69.14760810
GGACTGT4900.09.1117076
GTCTTGC6950.09.0314191
Produced by FastQC (version 0.11.5)