FastQCFastQC Report
Wed 25 May 2016
SRR1294997_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294997_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4089293
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC237400.5805404503908133No Hit
CCCATGTACTCTGCGTTGATACCAC217830.5326837670961704No Hit
GAGTACATGGGAAGCAGTGGTATCA114620.2802929503950927No Hit
CATGTACTCTGCGTTGATACCACTG83540.2042895923574075No Hit
GCGTTGATACCACTGCTTCCCATGT70570.17257261829856652No Hit
GTATCAACGCAGAGTACTTTTTTTT67420.16486957525420654No Hit
GTATCAACGCAGAGTACATGGGAAG64730.15829142103537214No Hit
GCTTCCCATGTACTCTGCGTTGATA62490.1528137015371606No Hit
ACGCAGAGTACATGGGAAGCAGTGG62220.15215344070478687No Hit
TATCAACGCAGAGTACATGGGAAGC55880.13664953819645598No Hit
GGTATCAACGCAGAGTACATGGGAA55550.13584255273466586No Hit
GGTATCAACGCAGAGTACTTTTTTT55090.13471766390914028No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN49270.12048537485575136No Hit
ACTCTGCGTTGATACCACTGCTTCC48720.11914039908610119No Hit
TATCAACGCAGAGTACTTTTTTTTT42630.10424784920033854No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCTC658.035542E-411.6910568
GTGTACG1158.697498E-710.7515551
TGAACCG856.5657386E-410.0641615
AGACCGT856.6050823E-410.0576236
AACCGCA1002.7599302E-49.4990997
GTATTAG6350.09.4361011
GTGTAAG5150.09.4186521
GTCTAGC2451.8189894E-129.3168891
TATACTG9500.09.104835
AGGTCGG1054.5007953E-49.04653910
GACAGTG7300.08.97860057
AACCGCT850.00744486978.9403297
TAGACAG5250.08.8713715
TCTGTCG3450.08.8106518
CTGTCGC3350.08.7901049
GTCTTAG4600.08.68394851
GTGTTAG6250.08.6740241
TGTCGCC3400.08.66073110
TATTCCG1107.110975E-48.6409465
AGTACGG1107.1196567E-48.6398873
Produced by FastQC (version 0.11.5)