##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294997_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4089293 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.11004200481599 33.0 33.0 33.0 27.0 33.0 2 31.152245143598172 33.0 33.0 33.0 27.0 33.0 3 31.098286182966103 33.0 33.0 33.0 27.0 33.0 4 34.61994921860576 37.0 37.0 37.0 27.0 37.0 5 34.93757233829906 37.0 37.0 37.0 33.0 37.0 6 34.91415459836211 37.0 37.0 37.0 33.0 37.0 7 34.79701968041908 37.0 37.0 37.0 33.0 37.0 8 34.68398180321146 37.0 37.0 37.0 27.0 37.0 9 34.60098677204103 37.0 37.0 37.0 27.0 37.0 10 34.52196822286884 37.0 37.0 37.0 27.0 37.0 11 34.7069625482938 37.0 37.0 37.0 27.0 37.0 12 34.61340163201805 37.0 37.0 37.0 27.0 37.0 13 34.53533459206763 37.0 37.0 37.0 27.0 37.0 14 35.920909311218345 40.0 37.0 40.0 27.0 40.0 15 36.08309284759004 40.0 37.0 40.0 27.0 40.0 16 36.165827931625344 40.0 37.0 40.0 27.0 40.0 17 36.08677514670629 40.0 37.0 40.0 27.0 40.0 18 36.0256435525652 40.0 37.0 40.0 27.0 40.0 19 36.119656625240594 40.0 37.0 40.0 27.0 40.0 20 36.12642918959341 40.0 37.0 40.0 27.0 40.0 21 36.12742544982714 40.0 37.0 40.0 27.0 40.0 22 36.022514894383946 40.0 37.0 40.0 27.0 40.0 23 35.92439426570803 40.0 37.0 40.0 27.0 40.0 24 35.98101896831555 40.0 37.0 40.0 27.0 40.0 25 34.82816002668432 37.0 33.0 40.0 22.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4399001529626432 1101 2 0.40885262480135154 1101 3 0.4142726466024982 1101 4 0.4473294386713249 1101 5 0.32485650386554 1101 6 0.34213368290564716 1101 7 0.3337271932982375 1101 8 0.41861553534592844 1101 9 0.48046445391796055 1101 10 0.6476429202309077 1101 11 0.4674826279717621 1101 12 0.5800106278973587 1101 13 0.48345818468708757 1101 14 0.9923672640223629 1101 15 0.846673250347088 1101 16 0.8033867070397633 1101 17 0.8386541938646914 1101 18 0.7801448889266993 1101 19 0.6397852530014063 1101 20 0.6113282694827546 1101 21 0.6814945270170369 1101 22 0.6942926231985638 1101 23 0.8194441636746603 1101 24 0.7462112046859275 1101 25 0.9315720471239857 1102 1 0.42835130507484465 1102 2 0.39650809619542216 1102 3 0.40591279194168095 1102 4 0.46976520311559256 1102 5 0.37704136525608334 1102 6 0.30345745505710653 1102 7 0.3745681928668887 1102 8 0.3625251427884777 1102 9 0.45888374772228957 1102 10 0.5964711845460897 1102 11 0.4683654938792472 1102 12 0.5109241078562974 1102 13 0.46750078689290575 1102 14 0.9346382915333535 1102 15 0.8565276576238432 1102 16 0.810505413191926 1102 17 0.7942904818999139 1102 18 0.8566977108561034 1102 19 0.672593110638843 1102 20 0.7504568297968319 1102 21 0.7781687528231913 1102 22 0.7680409609412351 1102 23 0.8798964056289904 1102 24 0.8496214541855167 1102 25 0.8769420746442975 1103 1 0.39994361017186364 1103 2 0.36624430058899193 1103 3 0.31726572172036427 1103 4 0.4065000169425801 1103 5 0.2909386372674305 1103 6 0.14788050566514954 1103 7 0.1948833144555593 1103 8 0.3153796054846012 1103 9 0.3457923267082066 1103 10 0.46171595573059676 1103 11 0.34224389236353403 1103 12 0.387797360275826 1103 13 0.41641105906673204 1103 14 0.7191428028337157 1103 15 0.6462273516305217 1103 16 0.6218837496416469 1103 17 0.6428872710020883 1103 18 0.6284107103828163 1103 19 0.6060717072152144 1103 20 0.5788303540551354 1103 21 0.5479315737292794 1103 22 0.5947407191419387 1103 23 0.665934008736123 1103 24 0.6593128531327253 1103 25 0.6774595487925197 1104 1 0.3840120912695433 1104 2 0.35655576948580503 1104 3 0.30794044724264324 1104 4 0.3836272028611276 1104 5 0.34064345248941663 1104 6 0.31761145479297426 1104 7 0.36420222988785866 1104 8 0.5219491716732634 1104 9 0.3988659408767603 1104 10 0.5241194569910945 1104 11 0.38574585483206647 1104 12 0.5367110511855557 1104 13 0.4728527299220886 1104 14 0.776717718780084 1104 15 0.7017683963022847 1104 16 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2216 1 -0.4829383767830322 2216 2 -0.4818583909525884 2216 3 -1.2687655931710893 2216 4 -0.6287027870029149 2216 5 -0.5239189600231597 2216 6 -0.40248657304070434 2216 7 -0.23979903949665982 2216 8 -0.4095289788478027 2216 9 -0.23543813973386563 2216 10 -0.2967622716664664 2216 11 -0.46125596131280844 2216 12 -0.2937448943846377 2216 13 -0.9381064358070574 2216 14 -0.5981529796267822 2216 15 -0.9699502185633335 2216 16 -0.8059619200731305 2216 17 -1.8437359141531715 2216 18 -0.7239173007189024 2216 19 -0.49728374339819226 2216 20 -0.5524157864344659 2216 21 -0.3232933490154082 2216 22 -0.3385993308652715 2216 23 -0.2930842978352288 2216 24 -0.47698365101756224 2216 25 -0.26404516627998476 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5455.0 3 511.0 4 661.0 5 644.0 6 431.0 7 330.0 8 613.0 9 1296.0 10 2542.0 11 4479.0 12 7292.0 13 10839.0 14 14472.0 15 18445.0 16 20771.0 17 22019.0 18 22705.0 19 23055.0 20 24295.0 21 25941.0 22 27413.0 23 29333.0 24 30550.0 25 32324.0 26 33660.0 27 37704.0 28 42466.0 29 49499.0 30 60237.0 31 75707.0 32 100884.0 33 140559.0 34 203266.0 35 324355.0 36 592467.0 37 2102073.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.09002813946637 21.154324035853474 13.627464472421188 23.128183352258972 2 14.70587226511454 21.141149072051093 40.80787437300915 23.34510428982522 3 18.22357862031281 26.693122146887948 29.890641424920428 25.192657807878817 4 12.010722993262101 18.400526052128765 38.592506649177345 30.996244305431787 5 12.222841629882186 39.34576332515029 35.68374025474815 12.747654790219373 6 30.17291420055636 39.28657976157252 17.699956164391615 12.840549873479507 7 25.540413297799486 33.48762804227113 23.218495175758513 17.75346348417087 8 23.97064078263798 37.747705223615405 21.159267063593013 17.12238693015361 9 23.847994002459107 17.81820088968098 22.209208597618975 36.124596510240934 10 14.160893023857746 31.197255024705317 34.246405112293445 20.395446839143496 11 31.34561400757463 26.36542781755024 23.251871113690004 19.037087061185122 12 20.428910970574464 28.52919849428053 31.834238527784525 19.20765200736049 13 26.416372950985405 24.219369034749093 26.616559559805136 22.747698454460366 14 19.78919385064185 24.550258404296795 28.493457245845672 27.167090499215686 15 22.968229569824892 31.125279002337386 24.147849859558512 21.758641568279213 16 20.596065908155843 32.016635582768565 26.840829612944823 20.546468896130772 17 19.94077768253721 31.053048670217848 28.75411613239648 20.252057514848463 18 20.135087515517935 30.369328081373038 29.70555499193533 19.7900294111737 19 20.46262372846966 30.029104508883158 29.926428839757584 19.581842922889592 20 21.529201378287606 29.41046888928857 29.197898753395606 19.862430979028208 21 21.30718211141942 29.69630127681763 28.867477366307583 20.129039245455374 22 20.535883408786 29.434111139018725 29.039543730894344 20.990461721300928 23 20.19892687603105 29.893677606035684 29.059918702135562 20.8474768157977 24 20.575713213958426 29.89139692364403 28.993940570384897 20.538949292012646 25 21.02742366460387 29.12977979262231 28.115413732198046 21.727382810575776 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 10002.0 1 10002.0 2 8714.0 3 7426.0 4 7426.0 5 7426.0 6 9909.5 7 12393.0 8 12393.0 9 12393.0 10 17314.0 11 22235.0 12 22235.0 13 22235.0 14 34915.0 15 47595.0 16 47595.0 17 47595.0 18 72732.5 19 97870.0 20 97870.0 21 97870.0 22 136083.5 23 174297.0 24 174297.0 25 174297.0 26 220871.0 27 267445.0 28 267445.0 29 267445.0 30 313320.0 31 359195.0 32 359195.0 33 359195.0 34 393358.0 35 427521.0 36 427521.0 37 427521.0 38 445667.0 39 463813.0 40 463813.0 41 463813.0 42 460297.0 43 456781.0 44 456781.0 45 456781.0 46 484681.5 47 512582.0 48 512582.0 49 512582.0 50 468550.0 51 424518.0 52 424518.0 53 424518.0 54 369889.5 55 315261.0 56 315261.0 57 315261.0 58 269596.5 59 223932.0 60 223932.0 61 223932.0 62 182722.5 63 141513.0 64 141513.0 65 141513.0 66 107699.0 67 73885.0 68 73885.0 69 73885.0 70 53233.5 71 32582.0 72 32582.0 73 32582.0 74 22468.0 75 12354.0 76 12354.0 77 12354.0 78 8322.5 79 4291.0 80 4291.0 81 4291.0 82 2800.5 83 1310.0 84 1310.0 85 1310.0 86 831.5 87 353.0 88 353.0 89 353.0 90 223.5 91 94.0 92 94.0 93 94.0 94 59.5 95 25.0 96 25.0 97 25.0 98 22.5 99 20.0 100 20.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3133059920137784 2 0.1832835162459623 3 0.20108610461514986 4 0.22226335946091413 5 0.24764672034994803 6 0.19895859748861236 7 0.193823235459039 8 0.2185952437255046 9 0.21896205529904558 10 0.22991749429546868 11 0.2029935247975628 12 0.2099629446948409 13 0.2225323546148442 14 0.21206599771647566 15 0.20135509976907986 16 0.20546338939273853 17 0.20539002707803036 18 0.20861796892519074 19 0.20741971778495696 20 0.2026511673289246 21 0.20074374714651164 22 0.2046319498260457 23 0.20778652935849792 24 0.22512448973453356 25 0.2179349828931309 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 4089293.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.49794515931019 #Duplication Level Percentage of deduplicated Percentage of total 1 89.17707075825845 70.89393880607955 2 8.55111617677818 13.59592329744804 3 1.3710397190266157 3.269845211832419 4 0.3663227021801053 1.1648760835409733 5 0.1535110680538946 0.6101907234747824 6 0.08440705541817872 0.40261124776159374 7 0.05204932122008778 0.2896469858753701 8 0.03560275434917194 0.22642766502166908 9 0.02875301919538217 0.20572253488431763 >10 0.15765254301146142 2.2635629426361263 >50 0.01192902772830237 0.6583641506107346 >100 0.008286108256556658 1.2482343024194567 >500 7.429305526882409E-4 0.3841947182191492 >1k 0.0011453508352048181 1.9629804521608694 >5k 2.7859885180656747E-4 1.4211410499559476 >10k+ 9.286628393552247E-5 1.4023398280790955 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 23740 0.5805404503908133 No Hit CCCATGTACTCTGCGTTGATACCAC 21783 0.5326837670961704 No Hit GAGTACATGGGAAGCAGTGGTATCA 11462 0.2802929503950927 No Hit CATGTACTCTGCGTTGATACCACTG 8354 0.2042895923574075 No Hit GCGTTGATACCACTGCTTCCCATGT 7057 0.17257261829856652 No Hit GTATCAACGCAGAGTACTTTTTTTT 6742 0.16486957525420654 No Hit GTATCAACGCAGAGTACATGGGAAG 6473 0.15829142103537214 No Hit GCTTCCCATGTACTCTGCGTTGATA 6249 0.1528137015371606 No Hit ACGCAGAGTACATGGGAAGCAGTGG 6222 0.15215344070478687 No Hit TATCAACGCAGAGTACATGGGAAGC 5588 0.13664953819645598 No Hit GGTATCAACGCAGAGTACATGGGAA 5555 0.13584255273466586 No Hit GGTATCAACGCAGAGTACTTTTTTT 5509 0.13471766390914028 No Hit NNNNNNNNNNNNNNNNNNNNNNNNN 4927 0.12048537485575136 No Hit ACTCTGCGTTGATACCACTGCTTCC 4872 0.11914039908610119 No Hit TATCAACGCAGAGTACTTTTTTTTT 4263 0.10424784920033854 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.445410490273013E-5 0.0 5 0.0 0.0 0.0 4.890820980546026E-5 0.0 6 0.0 0.0 0.0 4.890820980546026E-5 0.0 7 0.0 0.0 0.0 4.890820980546026E-5 0.0 8 0.0 0.0 0.0 4.890820980546026E-5 0.0 9 0.0 0.0 0.0 4.890820980546026E-5 0.0 10 0.0 0.0 0.0 4.890820980546026E-5 0.0 11 0.0 2.445410490273013E-5 0.0 4.890820980546026E-5 0.0 12 0.0 2.445410490273013E-5 0.0 4.890820980546026E-5 0.0 13 0.0 2.445410490273013E-5 0.0 4.890820980546026E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGCTC 65 8.035542E-4 11.691056 8 GTGTACG 115 8.697498E-7 10.751555 1 TGAACCG 85 6.5657386E-4 10.064161 5 AGACCGT 85 6.6050823E-4 10.057623 6 AACCGCA 100 2.7599302E-4 9.499099 7 GTATTAG 635 0.0 9.436101 1 GTGTAAG 515 0.0 9.418652 1 GTCTAGC 245 1.8189894E-12 9.316889 1 TATACTG 950 0.0 9.10483 5 AGGTCGG 105 4.5007953E-4 9.046539 10 GACAGTG 730 0.0 8.9786005 7 AACCGCT 85 0.0074448697 8.940329 7 TAGACAG 525 0.0 8.871371 5 TCTGTCG 345 0.0 8.810651 8 CTGTCGC 335 0.0 8.790104 9 GTCTTAG 460 0.0 8.6839485 1 GTGTTAG 625 0.0 8.674024 1 TGTCGCC 340 0.0 8.660731 10 TATTCCG 110 7.110975E-4 8.640946 5 AGTACGG 110 7.1196567E-4 8.639887 3 >>END_MODULE