FastQCFastQC Report
Wed 25 May 2016
SRR1294997_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294997_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4089293
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC244500.5979028648717516No Hit
GTACATGGGAAGCAGTGGTATCAAC232010.5673596878482418No Hit
GTATCAACGCAGAGTACTTTTTTTT158900.38857572690438175No Hit
GGTATCAACGCAGAGTACTTTTTTT137070.3351924159017219No Hit
GAGTACATGGGAAGCAGTGGTATCA126960.3104693158450617No Hit
TATCAACGCAGAGTACTTTTTTTTT109350.26740563711135396No Hit
CATGTACTCTGCGTTGATACCACTG94350.2307244797572588No Hit
ACGCAGAGTACATGGGAAGCAGTGG75970.1857778349460408No Hit
GCGTTGATACCACTGCTTCCCATGT75910.18563111031662444No Hit
GCTTCCCATGTACTCTGCGTTGATA70990.1735996907044812No Hit
GTATCAACGCAGAGTACATGGGAAG67570.1652363868277475No Hit
ACGCAGAGTACTTTTTTTTTTTTTT63420.1550879332931145No Hit
TATCAACGCAGAGTACATGGGAAGC61120.14946348916548655No Hit
GGTATCAACGCAGAGTACATGGGAA60800.1486809578085992No Hit
ACTCTGCGTTGATACCACTGCTTCC56170.13735870723863514No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAC707.275181E-613.57039813
CGTGCGC550.00307166412.08999210
GTACCGA600.00588176311.0824916
TCGCACT750.002650820410.1325654
TACGACT856.602453E-410.058064
AACGATT856.602453E-410.0580617
TATACTG9850.09.5474985
GGGCGCC1106.8477064E-59.49927911
CGTATAC1002.7594116E-49.4992783
AAGACGG1002.7594116E-49.4992785
AAGACCG1002.7594116E-49.4992785
CTCGGAA1252.7589158E-59.119307512
TAGACAG6600.09.0674935
CGCAAAA950.00182563828.99953652
GAACGAT950.00182604448.99931616
TAAGACG950.00182604448.9993164
TACACTG8800.08.9595475
GGCGTGC1701.6890954E-78.9404978
TACACCG850.0074437228.9404975
GTCTAAG3550.08.8428571
Produced by FastQC (version 0.11.5)