FastQCFastQC Report
Wed 25 May 2016
SRR1294996_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294996_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2419373
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC2188059.04387211066669No Hit
CCCATGTACTCTGCGTTGATACCAC2160938.931776952127679No Hit
GAGTACATGGGAAGCAGTGGTATCA1014884.1948058443241285No Hit
CATGTACTCTGCGTTGATACCACTG811573.3544641524890952No Hit
GCGTTGATACCACTGCTTCCCATGT666392.7543913237024635No Hit
ACGCAGAGTACATGGGAAGCAGTGG546382.2583537139581207No Hit
TATCAACGCAGAGTACATGGGAAGC544042.248681786562056No Hit
GTATCAACGCAGAGTACATGGGAAG531892.1984621635440256No Hit
ACTCTGCGTTGATACCACTGCTTCC491252.030484757827751No Hit
GGTATCAACGCAGAGTACATGGGAA464361.9193402588191237No Hit
GCTTCCCATGTACTCTGCGTTGATA385101.5917347180447166No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA264731.09420911947021No Hit
GTACTCTGCGTTGATACCACTGCTT246381.0183630221549138No Hit
GTGGTATCAACGCAGAGTACATGGG239520.9900085683356803No Hit
ATACCACTGCTTCCCATGTACTCTG238320.9850486055684676No Hit
CAGTGGTATCAACGCAGAGTACATG225940.9338783230200551No Hit
GGGAAGCAGTGGTATCAACGCAGAG216460.894694617159074No Hit
ACATGGGAAGCAGTGGTATCAACGC209930.867704153100824No Hit
GCAGTGGTATCAACGCAGAGTACAT203840.842532342057219No Hit
CATGGGAAGCAGTGGTATCAACGCA195320.8073166064100079No Hit
TACCACTGCTTCCCATGTACTCTGC194910.8056219524645435No Hit
GTTGATACCACTGCTTCCCATGTAC193240.7987193376135058No Hit
CTGCTTCCCATGTACTCTGCGTTGA193220.7986366715673855No Hit
GATACCACTGCTTCCCATGTACTCT192930.7974380138986423No Hit
GTACATGGTAAGCAGTGGTATCAAC185240.7656529191654202No Hit
CCATGTACTCTGCGTTGATACCACT183870.7599902950061855No Hit
GCAGAGTACATGGGAAGCAGTGGTA174080.7195252654303408No Hit
CTTCCCATGTACTCTGCGTTGATAC169400.7001814106382108No Hit
CCACTGCTTCCCATGTACTCTGCGT164720.6808375558460809No Hit
AAGCAGTGGTATCAACGCAGAGTAC158910.6568230694481586No Hit
TTCCCATGTACTCTGCGTTGATACC148230.6126794008199645No Hit
ACCATGTACTCTGCGTTGATACCAC147130.6081327682833527No Hit
ATGGGAAGCAGTGGTATCAACGCAG139540.5767610037807317No Hit
TGATACCACTGCTTCCCATGTACTC121010.5001709120503535No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA118520.48987898930838697No Hit
ATCAACGCAGAGTACATGGGAAGCA102210.4224648286973526No Hit
GAACAAAAAAAAAAAAAAAAAAAAA81770.33798012956249407No Hit
GGTAAGCAGTGGTATCAACGCAGAG78590.32483622822938013No Hit
CCCCATGTACTCTGCGTTGATACCA76740.3171896189632603No Hit
GTACATGGGTAAGCAGTGGTATCAA72640.3002430795086165No Hit
TCCATGTACTCTGCGTTGATACCAC71150.29408445907266056No Hit
GAGTACATGGTAAGCAGTGGTATCA65810.27201262475856347No Hit
GGAAGCAGTGGTATCAACGCAGAGT63930.2642420164232634No Hit
AAAAAGTACTCTGCGTTGATACCAC61780.25535541646534043No Hit
AAACAAAAAAAAAAAAAAAAAAAAA60250.24903146393714404No Hit
GCTTACCATGTACTCTGCGTTGATA58780.24295550954730832No Hit
GTATCAACGCAGAGTACTTTTTTTT55640.22997694030643476No Hit
GTACATGGAAAGCAGTGGTATCAAC50600.20914509668414089No Hit
CTGCGTTGATACCACTGCTTCCCAT49340.20393713577856742No Hit
CTCTGCGTTGATACCACTGCTTCCC49140.20311047531736529No Hit
GCGTTGATACCACTGCTTACCATGT46420.1918678930450162No Hit
TCCCATGTACTCTGCGTTGATACCA46250.1911652316529944No Hit
TGGGAAGCAGTGGTATCAACGCAGA45950.18992524096119118No Hit
GGTATCAACGCAGAGTACTTTTTTT44680.1846759470325576No Hit
ACTCTGCGTTGATACCACTGCTTAC43840.18120397309550862No Hit
AAAGTACTCTGCGTTGATACCACTG43450.17959198519616446No Hit
TCAACGCAGAGTACATGGGAAGCAG43300.17897198985026286No Hit
TATCAACGCAGAGTACTTTTTTTTT43260.17880665775802243No Hit
GTACTTTTTTTTTTTTTTTTTTTTT41880.17310270057572769No Hit
ACGCAGAGTACATGGTAAGCAGTGG39910.1649600950328866No Hit
AGTGGTATCAACGCAGAGTACATGG37830.1563628262363844No Hit
CTGCTTACCATGTACTCTGCGTTGA37010.1529735183454556No Hit
GAGTACATGGAAAGCAGTGGTATCA36270.1499148746390077No Hit
GTATCAACGCAGAGTACATGGTAAG36090.1491708802239258No Hit
TATCAACGCAGAGTACATGGTAAGC35910.14842688580884386No Hit
ATGGTAAGCAGTGGTATCAACGCAG34440.14235093141900815No Hit
AAAAACAAAAAAAAAAAAAAAAAAA33760.13954028585092088No Hit
GGTATCAACGCAGAGTACATGGTAA33220.13730830260567511No Hit
TTGATACCACTGCTTCCCATGTACT33160.13706030446731446No Hit
TAAAAAAAAAAAAAAAAAAAAAAAA31840.13160434542338037No Hit
AACGCAGAGTACATGGGAAGCAGTG30920.12780170730185053No Hit
GTACATGGAAGCAGTGGTATCAACG30860.12755370916348988No Hit
ACTGCTTCCCATGTACTCTGCGTTG29780.12308974267299833No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN29420.12160175384283449No Hit
ATGTACTCTGCGTTGATACCACTGC29300.1211057575661132No Hit
GCTTTCCATGTACTCTGCGTTGATA28710.11866710920556689No Hit
GAAAACAAAAAAAAAAAAAAAAAAA26490.10949117808622318No Hit
CGTTGATACCACTGCTTCCCATGTA25620.10589520507999386No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATAA8350.017.186052
TCTAGAA350.002165265416.291792
GTACAAA9750.016.189121
GTACATA10350.016.077461
AGAACAA15650.015.24210552
TACAAAA11850.014.2753672
GTACCGG400.00524836414.2630051
TACATGA7250.014.0259212
TACAAGA1850.013.8700382
GGACAGC551.9618933E-413.8173236
GTAAAAA4700.013.7572241
ACATAAA17200.013.3162893
TTGCAAC500.00148969113.3121371
CCGGGGT500.00149435413.3066184
GACCACT500.00150066913.2991748
CGCAAAA6200.013.0290532
CGCAGAA5700.012.8381222
GAGAACA22800.012.7616361
TTTCAAC905.4227166E-712.664041519
CATAAAA17200.012.3229894
Produced by FastQC (version 0.11.5)