##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294996_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2419373
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.99826194638032	33.0	33.0	33.0	15.0	33.0
2	29.971540973632425	33.0	33.0	33.0	15.0	33.0
3	30.296940984296345	33.0	33.0	33.0	22.0	33.0
4	33.2043455060464	37.0	33.0	37.0	15.0	37.0
5	34.333579815927514	37.0	33.0	37.0	27.0	37.0
6	33.73534258669498	37.0	37.0	37.0	22.0	37.0
7	34.00795908692045	37.0	37.0	37.0	27.0	37.0
8	33.78326368030064	37.0	37.0	37.0	22.0	37.0
9	33.57355893448427	37.0	33.0	37.0	22.0	37.0
10	33.83144227863996	37.0	33.0	37.0	27.0	37.0
11	33.97368574419902	37.0	37.0	37.0	27.0	37.0
12	34.06686567139503	37.0	37.0	37.0	27.0	37.0
13	33.62450271206631	37.0	33.0	37.0	22.0	37.0
14	35.171679604591766	37.0	33.0	40.0	27.0	40.0
15	35.03548357363664	37.0	33.0	40.0	22.0	40.0
16	35.037430772353005	37.0	33.0	40.0	27.0	40.0
17	35.146665685696256	37.0	33.0	40.0	27.0	40.0
18	34.985918665703885	37.0	33.0	40.0	22.0	40.0
19	34.81928954319983	37.0	33.0	40.0	22.0	40.0
20	34.972649111980665	37.0	33.0	40.0	22.0	40.0
21	34.87208338689404	37.0	33.0	40.0	22.0	40.0
22	34.95386986628354	37.0	33.0	40.0	22.0	40.0
23	34.983115460079944	37.0	33.0	40.0	22.0	40.0
24	35.1450954441502	37.0	33.0	40.0	27.0	40.0
25	34.00757138316415	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.5878557284538246
1101	2	0.5836497776529264
1101	3	0.5466844457618123
1101	4	0.6295460787676461
1101	5	0.3368402978897933
1101	6	0.41355637208089036
1101	7	0.2522986945461483
1101	8	0.26485966349733303
1101	9	0.32716475274317247
1101	10	0.5841041403728227
1101	11	0.4301264708346366
1101	12	0.46340686013282806
1101	13	0.5691151661129652
1101	14	1.0395873470833976
1101	15	0.8716844506385684
1101	16	0.9125606714166281
1101	17	0.7595945011067755
1101	18	0.9255587740598301
1101	19	0.9010138350605743
1101	20	0.8252902807833848
1101	21	0.7377315535326474
1101	22	0.794152118919115
1101	23	0.9291684341701583
1101	24	0.9663278332680036
1101	25	1.1314379932814163
1102	1	0.48953646202180323
1102	2	0.3928953515020268
1102	3	0.34988931346122243
1102	4	0.460737825575805
1102	5	0.31127293026776215
1102	6	0.23435199091049697
1102	7	0.3304259192076131
1102	8	0.28541743856734314
1102	9	0.36836788814496657
1102	10	0.5847019286065489
1102	11	0.36786865830457316
1102	12	0.4356912694710431
1102	13	0.3797138360550605
1102	14	0.839981288358274
1102	15	0.7747824425686787
1102	16	0.8005511677046684
1102	17	0.7988106975038889
1102	18	0.85022331916106
1102	19	0.8435584052511729
1102	20	0.8002913390007294
1102	21	0.7653439157197042
1102	22	0.6690578622871044
1102	23	0.9808955201675076
1102	24	0.9064428535464586
1102	25	1.0586348985017793
1103	1	0.5027111541705587
1103	2	0.4070308022362923
1103	3	0.3973989958908355
1103	4	0.4884976800649312
1103	5	0.36607965609710647
1103	6	0.30463591933082057
1103	7	0.37151388541020225
1103	8	0.38996982948265924
1103	9	0.46248263737943063
1103	10	0.5401312328143462
1103	11	0.3327370383538053
1103	12	0.4263367325965177
1103	13	0.3934489842339346
1103	14	0.7853148387993585
1103	15	0.758014519800625
1103	16	0.7102414021640016
1103	17	0.7182122849379908
1103	18	0.7358416912796812
1103	19	0.74624902419243
1103	20	0.6592927553123857
1103	21	0.6060366419691476
1103	22	0.6404750104080534
1103	23	0.7839694608524681
1103	24	0.7743890117980499
1103	25	0.751563327504094
1104	1	0.565597397345261
1104	2	0.48325278711212505
1104	3	0.5206505494308509
1104	4	0.5652677352003721
1104	5	0.48314132363954343
1104	6	0.5177007910495419
1104	7	0.44803525039310443
1104	8	0.5153563106383743
1104	9	0.4700268732890507
1104	10	0.6625367638737245
1104	11	0.5717608783391839
1104	12	0.6508415893129111
1104	13	0.6301803531768471
1104	14	1.0046183826221338
1104	15	0.969271687788563
1104	16	0.7909300525641854
1104	17	0.8911402319043944
1104	18	0.9533813198770034
1104	19	0.89326083947347
1104	20	0.9208353101241755
1104	21	0.7289109956127291
1104	22	0.81561427689347
1104	23	0.8950604229049688
1104	24	0.9607893015637288
1104	25	1.1050469886800656
1105	1	0.5705786285764951
1105	2	0.578860895220231
1105	3	0.47057172060202035
1105	4	0.6445207382033757
1105	5	0.32598291523112977
1105	6	0.35344403429278515
1105	7	0.3778100251678822
1105	8	0.5900635178455786
1105	9	0.6406912877034614
1105	10	0.7205022293391892
1105	11	0.42557469215299193
1105	12	0.5486981959195205
1105	13	0.6078342570807465
1105	14	0.989877391631147
1105	15	0.8792791952960641
1105	16	0.8169923648764978
1105	17	0.6379570487212121
1105	18	0.6472063949520788
1105	19	0.763516094728729
1105	20	0.8648443191331836
1105	21	0.7509380226397582
1105	22	0.7592554180346127
1105	23	0.896501864346412
1105	24	0.9187209832454073
1105	25	0.8537181598512404
1106	1	0.2904020461260721
1106	2	0.15650963197654733
1106	3	0.0765252658342952
1106	4	0.3624418194313037
1106	5	-0.16758405026737222
1106	6	-0.1455861205564446
1106	7	-0.507935696103516
1106	8	-0.04923762314871283
1106	9	0.079454531387249
1106	10	-0.03192374186428992
1106	11	-0.3114675518024512
1106	12	-0.8621949037816492
1106	13	0.042293643117155
1106	14	-0.03066254316917849
1106	15	0.07140361800728812
1106	16	0.10603542382787623
1106	17	-0.017168596483891463
1106	18	-0.11981343661543065
1106	19	-0.4443085453147404
1106	20	0.14240544382755616
1106	21	0.09110509680438383
1106	22	0.3571391452826447
1106	23	-0.3981660248141452
1106	24	0.27846493869229505
1106	25	0.4459410612367307
1107	1	-0.9314435936457279
1107	2	-1.0248057714464345
1107	3	-0.6748949460646436
1107	4	-0.7808237062410655
1107	5	-0.7590393069910917
1107	6	-0.7926892990405534
1107	7	-0.9162566414987836
1107	8	-0.8087031488210883
1107	9	-0.9379420456298675
1107	10	-0.783186659549699
1107	11	-0.7697030856247835
1107	12	-0.8628462485249244
1107	13	-0.7374101873636931
1107	14	-1.0875559417021918
1107	15	-0.9310985824817095
1107	16	-1.0362631906790511
1107	17	-1.0089651735010108
1107	18	-1.3151047161590341
1107	19	-1.1792283497157143
1107	20	-1.2524556808668166
1107	21	-1.140595310693577
1107	22	-0.9285001375209418
1107	23	-0.7901981356535899
1107	24	-0.7658012389768132
1107	25	-0.8323151734820939
1108	1	-0.016277802816258458
1108	2	-0.2364018559389649
1108	3	-0.12381776935161071
1108	4	-0.1842784203059864
1108	5	-0.4986452832919639
1108	6	-0.2624652907973797
1108	7	-0.1191528805508213
1108	8	-0.082884704720108
1108	9	-0.33028372517649274
1108	10	-0.538675170626469
1108	11	-0.18725074147228327
1108	12	-0.06439514084847531
1108	13	-0.15741389679182305
1108	14	-0.2679446073384568
1108	15	-0.1003610245218951
1108	16	-0.766246086093787
1108	17	-0.7517394135834436
1108	18	-0.7188763802497036
1108	19	-0.12427069902020094
1108	20	-0.952787056446418
1108	21	-0.28965703145607336
1108	22	-0.4699039499887405
1108	23	-0.29561431288182405
1108	24	-0.5838245259422976
1108	25	-1.4685010570472699
1109	1	-0.07146079794680205
1109	2	-0.12867673918836786
1109	3	0.034765268989119136
1109	4	0.007898874404091316
1109	5	0.031180047847620074
1109	6	-0.14053446033087624
1109	7	-9.320063857387595E-5
1109	8	-0.07949024559528084
1109	9	0.032019244692712334
1109	10	0.07765635120298242
1109	11	-0.12256330067855004
1109	12	0.020044074055718397
1109	13	-0.08762094005187038
1109	14	0.273398897007489
1109	15	0.060694965905383924
1109	16	-0.027084696055396762
1109	17	0.14920256843447532
1109	18	0.11884797201301467
1109	19	0.13641035996170103
1109	20	0.03492496429446845
1109	21	0.13881974306986677
1109	22	0.13594717430701309
1109	23	0.14749648800232507
1109	24	0.24850413736727006
1109	25	0.4582396652275804
1110	1	-0.130518151055945
1110	2	-0.21563834605442622
1110	3	0.014643546601011082
1110	4	-1.2159087846426857
1110	5	-0.46537941827080687
1110	6	-0.27152308760908994
1110	7	-0.17488850796312505
1110	8	-0.1263869120987806
1110	9	-0.09755479221560392
1110	10	-0.23252929991389237
1110	11	-0.15696661622670405
1110	12	0.026632355420446174
1110	13	0.03392092037530148
1110	14	0.12634552516680486
1110	15	-0.21719104580998305
1110	16	-0.11706723721420786
1110	17	-0.05179046434572143
1110	18	0.01849285591987382
1110	19	-0.027118283959516987
1110	20	-0.03598142694674067
1110	21	-0.11735484534203522
1110	22	-0.5978201172882649
1110	23	-0.1426015752084666
1110	24	0.018614340183532363
1110	25	-0.02954815274360101
1111	1	-0.2266134504656243
1111	2	-0.3986384767968474
1111	3	-0.17539914099054243
1111	4	-0.19927876778339737
1111	5	-0.2579750517788355
1111	6	-0.14220530004532606
1111	7	-0.12892320768818877
1111	8	-0.03965074992633788
1111	9	-0.143951390015701
1111	10	-0.24636455455095785
1111	11	-0.24253905648637897
1111	12	-0.18015908449579143
1111	13	-0.34273427678846247
1111	14	-0.3708943993761409
1111	15	-0.2373368140818144
1111	16	-0.3549993876144981
1111	17	-0.242960852978527
1111	18	-0.36239755715375566
1111	19	-0.3630754233153155
1111	20	-0.20358635155257332
1111	21	-0.3593596412637865
1111	22	-0.24582663840011776
1111	23	0.0026818442509579654
1111	24	-0.13656014377460224
1111	25	-0.15181499764688056
1112	1	-0.3582032866066953
1112	2	-0.7007038606184075
1112	3	-0.2747541000741158
1112	4	-0.5685579541587558
1112	5	-0.2868664505481675
1112	6	-0.43553849061331107
1112	7	-0.4105804696875808
1112	8	-0.28186650011947023
1112	9	-0.49612685699684533
1112	10	-0.1058666530169603
1112	11	-0.2577612270587224
1112	12	-0.2723251314254682
1112	13	-0.5191252943373996
1112	14	-0.5863185090711269
1112	15	-0.6208479210412392
1112	16	-0.6014486226568465
1112	17	-0.6228822729620589
1112	18	-0.4967644254518646
1112	19	-0.4821422154677961
1112	20	-1.1757105559280632
1112	21	-1.6454175220278913
1112	22	-1.8946824664446709
1112	23	-0.7895903290228503
1112	24	-0.5929137001886389
1112	25	-1.597536535559506
1113	1	-0.3636667538702163
1113	2	-0.29215127773052174
1113	3	-0.37025588195903936
1113	4	-0.7913162839029155
1113	5	-0.3799931604414084
1113	6	-0.39232942235096147
1113	7	-0.4547451088136256
1113	8	-0.36356603713031177
1113	9	-0.4564581471238185
1113	10	-0.2532439830814397
1113	11	-0.4521439993865428
1113	12	-0.34128988143932304
1113	13	-0.5261588396441468
1113	14	-0.7414883106848791
1113	15	-0.7402474560885395
1113	16	-0.6990649296720193
1113	17	-0.812388839614357
1113	18	-1.1550674700915238
1113	19	-1.281477911015216
1113	20	-1.3386967052917669
1113	21	-1.1590094123456183
1113	22	-0.48526662238379714
1113	23	-1.9681035028182379
1113	24	-1.1343393150182308
1113	25	-0.6210231649452211
1114	1	-0.5067999047047351
1114	2	-0.606944106733657
1114	3	-0.4397799792807433
1114	4	-0.7372888879526727
1114	5	-0.8311865520086741
1114	6	-0.5530897679737308
1114	7	-0.49073042031863423
1114	8	-0.34266704096645384
1114	9	-1.32870593777524
1114	10	-0.5098364446219961
1114	11	-0.6277882751519428
1114	12	-0.6123552754885822
1114	13	-0.7556879521155508
1114	14	-0.9462373016084058
1114	15	-1.458867867358613
1114	16	-1.472723995681008
1114	17	-1.6969586084365318
1114	18	-1.9604658728608655
1114	19	-1.9130590648388122
1114	20	-1.0306334065409075
1114	21	-0.8577391950233917
1114	22	-0.6607530488144135
1114	23	-0.48753584569656994
1114	24	-1.0869488747720482
1114	25	-0.6505501090037811
1115	1	-0.8792879227317769
1115	2	-0.7648114344950407
1115	3	-0.6976161038434725
1115	4	-0.8093231871282782
1115	5	-0.5953568152497013
1115	6	-0.6537071376436643
1115	7	-0.5993938445820035
1115	8	-0.4868709638281672
1115	9	-0.7927305307643664
1115	10	-0.5004945720870637
1115	11	-0.8564452596062182
1115	12	-0.8669602645702312
1115	13	-0.7686315811028805
1115	14	-0.8179804815794895
1115	15	-1.8747066995180504
1115	16	-1.205474076316932
1115	17	-0.978074753491569
1115	18	-2.454120226619942
1115	19	-1.5788153247039176
1115	20	-1.3707986728441668
1115	21	-1.226207643554993
1115	22	-0.8537651367344097
1115	23	-0.6538139292669243
1115	24	-0.6915030480062327
1115	25	-0.8652794018917263
1116	1	-1.7576802762613575
1116	2	-1.2934277810158115
1116	3	-1.851390001886017
1116	4	-1.4439069598295866
1116	5	-1.3679139481394174
1116	6	-0.8940998253563421
1116	7	-1.707811984401495
1116	8	-3.3891988426009902
1116	9	-1.9472473087877624
1116	10	-1.391847584589577
1116	11	-1.0456520489421663
1116	12	-1.1779352437402366
1116	13	-1.995158831701815
1116	14	-2.330740630697406
1116	15	-1.9888574341563512
1116	16	-1.5243327281591164
1116	17	-2.211573785941198
1116	18	-2.9717197081845512
1116	19	-2.0835479669405927
1116	20	-1.4942585516802183
1116	21	-1.1614463473761916
1116	22	-1.0128152890360909
1116	23	-0.8815404004063794
1116	24	-0.981415912023067
1116	25	-1.2671630203720454
1201	1	0.6613028868661956
1201	2	0.5602017910899235
1201	3	0.5736025524810202
1201	4	0.5416018900358353
1201	5	0.34522084425963584
1201	6	0.2979931907791098
1201	7	0.1240607523348416
1201	8	0.16286311063208814
1201	9	0.22295568560922163
1201	10	0.40603451555210057
1201	11	0.26831902025072907
1201	12	0.38406614240003023
1201	13	0.34860214538790757
1201	14	0.7333227086759351
1201	15	0.8175659899441214
1201	16	0.7697454189777204
1201	17	0.8392470429038781
1201	18	0.6849609033872142
1201	19	0.7637356991499047
1201	20	0.7102035396102053
1201	21	0.6206849685385336
1201	22	0.7883799090177419
1201	23	0.8453369428804436
1201	24	0.9633152240831038
1201	25	1.1075154257034754
1202	1	0.5800461543827389
1202	2	0.6008699354202705
1202	3	0.46351459423156527
1202	4	0.6572762360555302
1202	5	0.4704600582714633
1202	6	0.22481929876671813
1202	7	0.2650777689647654
1202	8	0.39093724356206394
1202	9	0.4058216012252984
1202	10	0.678801261204292
1202	11	0.3928397110668911
1202	12	0.4338743515904824
1202	13	0.38381297093597766
1202	14	0.8285737755511278
1202	15	0.8481286535252153
1202	16	0.7391599291516826
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2211	1	0.045501960765530924
2211	2	-0.121971579891337
2211	3	-0.03587524129293129
2211	4	-0.036028237651983375
2211	5	0.023565018446603858
2211	6	-0.013236363922118244
2211	7	0.08635276461175323
2211	8	0.044439590622786795
2211	9	0.16236567836601523
2211	10	0.3414348303232799
2211	11	0.05666835275489035
2211	12	0.12070843736590575
2211	13	-0.02738945272586335
2211	14	0.0034467359497156735
2211	15	-0.032654092265438805
2211	16	-0.04473294694407315
2211	17	0.05776108808498748
2211	18	0.2696345903977857
2211	19	0.031118509192623378
2211	20	0.018863371656351546
2211	21	0.030311520751666876
2211	22	0.12019660123635134
2211	23	0.1715730967528657
2211	24	0.17023803325113107
2211	25	0.08783848254262239
2212	1	0.009102827622164966
2212	2	0.03921876843086736
2212	3	0.210755769681775
2212	4	0.0599075821024897
2212	5	0.2904478095733296
2212	6	0.15814751077540024
2212	7	0.09992658958505984
2212	8	0.3850686303200206
2212	9	0.15559415068914717
2212	10	0.3845172259308711
2212	11	0.2703787139662168
2212	12	0.3649132606775254
2212	13	0.17778654065266153
2212	14	0.17797043866590911
2212	15	0.1328817856164477
2212	16	0.1220860935745236
2212	17	0.1982348264989895
2212	18	0.17188260490436136
2212	19	0.1054980306523845
2212	20	0.18743634367305617
2212	21	0.2401916013718619
2212	22	-0.34849850152912154
2212	23	0.12343949215827621
2212	24	0.17670837247512594
2212	25	0.2302467897080902
2213	1	-0.23399606573504173
2213	2	-0.8642411781449972
2213	3	-0.230815839206592
2213	4	-0.2790744239444152
2213	5	-0.06456466584276654
2213	6	-0.1859658540644773
2213	7	-0.16929523799001345
2213	8	-0.0018782684383111814
2213	9	-0.20657745232843183
2213	10	-1.4205557185608697
2213	11	-0.43034104415747976
2213	12	-0.14037543512780104
2213	13	-0.23630715706066496
2213	14	-0.15657795127156504
2213	15	-0.1879004963573081
2213	16	-0.26308870902035864
2213	17	-0.23262641752014446
2213	18	-0.2037771244524933
2213	19	-0.23761495258652587
2213	20	-0.12870831244576664
2213	21	-0.1549870172326493
2213	22	-0.2372172029116939
2213	23	-1.1846504482532723
2213	24	-1.7510331369034517
2213	25	-1.5001773744390334
2214	1	0.02113155241459097
2214	2	-0.13857554880313572
2214	3	-0.035070155286181404
2214	4	0.05864687642117872
2214	5	0.012017443364193525
2214	6	-0.04861502144699159
2214	7	0.06992039939810724
2214	8	0.12618344521018088
2214	9	-0.2849146652414305
2214	10	-0.009771469276529388
2214	11	-0.09466439937813931
2214	12	-0.04184880655429879
2214	13	-0.1039937338661403
2214	14	-0.06276314208115963
2214	15	-0.1362366213258639
2214	16	-0.24808040211123483
2214	17	-0.19679122750385147
2214	18	-0.1930536187328542
2214	19	-0.7767135226195521
2214	20	-0.3899389589340885
2214	21	-0.35246241948877355
2214	22	-0.5405832576851637
2214	23	-1.7647041201790898
2214	24	-1.3385790062277252
2214	25	-1.7300190105261493
2215	1	-0.5940089976274479
2215	2	-0.46481599687055564
2215	3	-1.3215337871832453
2215	4	-1.09766888402401
2215	5	-0.5375151610464002
2215	6	-0.575134476823493
2215	7	-0.3244244623989303
2215	8	-0.2106904135201333
2215	9	-0.6097899656856427
2215	10	-1.6735590199184358
2215	11	-0.7602554134580757
2215	12	-0.6616868713462125
2215	13	-0.7633527700579421
2215	14	-1.1002297041934739
2215	15	-1.2836124565230804
2215	16	-0.9069257671760695
2215	17	-0.7713677763362412
2215	18	-0.6808153986286243
2215	19	-1.232818310208394
2215	20	-1.7768864500975852
2215	21	-1.0255322694045574
2215	22	-0.6674198989277116
2215	23	-0.9026287809398212
2215	24	-1.9965655739369197
2215	25	-0.9920442927012445
2216	1	-0.6012417491134485
2216	2	-0.5929034231553381
2216	3	-1.430569874375891
2216	4	-0.9139047571594574
2216	5	-0.7768414925293357
2216	6	-0.62619945946836
2216	7	-0.5884547969665093
2216	8	-0.6168246089466365
2216	9	-0.6190922897591378
2216	10	-0.5416401664860757
2216	11	-0.6338959089991931
2216	12	-0.7275173482691741
2216	13	-1.4397598424826583
2216	14	-1.1331868182425282
2216	15	-1.3662305052903605
2216	16	-1.2028193714122182
2216	17	-2.0694703583417464
2216	18	-1.2486601993799908
2216	19	-1.060939393658984
2216	20	-0.8300225722874188
2216	21	-0.6993824454684372
2216	22	-0.7474816456973343
2216	23	-0.455934972048226
2216	24	-0.6818956046282096
2216	25	-0.7006864359791862
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	3251.0
3	355.0
4	397.0
5	367.0
6	256.0
7	144.0
8	227.0
9	453.0
10	831.0
11	1673.0
12	3280.0
13	5990.0
14	11310.0
15	20452.0
16	28510.0
17	30396.0
18	28510.0
19	27235.0
20	26886.0
21	26966.0
22	27049.0
23	25831.0
24	25759.0
25	25438.0
26	24964.0
27	25946.0
28	26730.0
29	29127.0
30	34702.0
31	43821.0
32	57703.0
33	80035.0
34	114909.0
35	183923.0
36	346773.0
37	1129174.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.529395910819204	18.27816546628354	9.712447081555563	23.479991541341693
2	9.085736576251561	28.012076880583653	31.090720711990993	31.81146583117379
3	23.833005650611387	35.96005515175638	22.77577845527866	17.431160742353573
4	13.219550752267489	26.304713281649377	23.446146529560853	37.02958943652227
5	8.437517222403857	46.69489379811137	32.93599046277661	11.931598516708167
6	31.991544377110177	27.766383597681994	19.87361295645288	20.368459068754948
7	19.928345010780138	38.60759388824604	21.54789188884738	19.916169212126434
8	29.334988102891757	36.75576794342411	20.8754482127826	13.03379574090153
9	28.39625638920429	22.399735214887286	16.27450826065758	32.92950013525085
10	17.967910760943912	38.17475208496369	27.5216036251088	16.335733528983596
11	22.56826002176899	34.22527091123256	12.777807230153195	30.428661836845254
12	25.280767169315215	40.23530016013069	22.894712207956168	11.589220462597929
13	30.865213735050563	24.805030717034597	16.523610483974533	27.806145063940313
14	20.53003558450585	36.674615295082994	20.83095682184096	21.9643922985702
15	41.125206926300066	21.388175722828716	17.15708424121363	20.329533109657582
16	13.580059766030281	30.45880679170731	37.13794024639509	18.823193195867322
17	29.525113599228632	34.44548955872845	23.962634334116547	12.06676250792637
18	30.35044468302252	24.73872387238724	27.269598194759237	17.641233249831007
19	22.623642730960956	38.55834884843113	28.284171700875817	10.533836719732099
20	24.328422437290502	30.240839752040543	24.425132754065455	21.005605056603493
21	15.318944673293867	34.85049041331227	31.26729658470885	18.563268328685016
22	20.48567277329914	33.87979907423054	13.558637588095547	32.075890564374774
23	12.403601340831363	35.62481076475682	25.31380432527048	26.65778356914133
24	19.427003846045498	46.81392165732911	17.008933231921016	16.75014126470437
25	20.330099363686337	26.850812800505043	14.481614176278065	38.33747365953056
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	35135.0
1	35135.0
2	37312.0
3	39489.0
4	39489.0
5	39489.0
6	43267.5
7	47046.0
8	47046.0
9	47046.0
10	42450.0
11	37854.0
12	37854.0
13	37854.0
14	34638.5
15	31423.0
16	31423.0
17	31423.0
18	30293.0
19	29163.0
20	29163.0
21	29163.0
22	29389.5
23	29616.0
24	29616.0
25	29616.0
26	31956.0
27	34296.0
28	34296.0
29	34296.0
30	35582.5
31	36869.0
32	36869.0
33	36869.0
34	37676.0
35	38483.0
36	38483.0
37	38483.0
38	43427.0
39	48371.0
40	48371.0
41	48371.0
42	104936.0
43	161501.0
44	161501.0
45	161501.0
46	580494.5
47	999488.0
48	999488.0
49	999488.0
50	814707.5
51	629927.0
52	629927.0
53	629927.0
54	394025.5
55	158124.0
56	158124.0
57	158124.0
58	93492.5
59	28861.0
60	28861.0
61	28861.0
62	22428.5
63	15996.0
64	15996.0
65	15996.0
66	12727.0
67	9458.0
68	9458.0
69	9458.0
70	7137.0
71	4816.0
72	4816.0
73	4816.0
74	3494.5
75	2173.0
76	2173.0
77	2173.0
78	1498.0
79	823.0
80	823.0
81	823.0
82	574.0
83	325.0
84	325.0
85	325.0
86	214.5
87	104.0
88	104.0
89	104.0
90	60.5
91	17.0
92	17.0
93	17.0
94	11.5
95	6.0
96	6.0
97	6.0
98	7.5
99	9.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.315908295248397
2	0.1864532670241422
3	0.2043504660091685
4	0.2261329691618448
5	0.2518007764821712
6	0.20191181764862218
7	0.19620786046632743
8	0.22075967616403094
9	0.2207183431409708
10	0.22906761379911245
11	0.20373047066326688
12	0.21059175249124465
13	0.22332232359375753
14	0.21162507806774733
15	0.20112649021048015
16	0.20492912833201
17	0.20517712647037065
18	0.20951709389168183
19	0.20906243063802069
20	0.2044331320552887
21	0.20166381951026152
22	0.20455713112446902
23	0.2087317664535398
24	0.22588497102348418
25	0.21956101849528784
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2419373.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.296362504209144
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.22564488350191	9.618908866130727
2	10.315459148212195	2.5368525016755528
3	4.052286983171538	1.4948516914849583
4	2.067629632856022	1.0169729396017002
5	1.2051875050504735	0.7409711223822004
6	0.8171901351526355	0.6029079682020282
7	0.5154814162355952	0.4436982450751199
8	0.37446726976306033	0.3683668235974452
9	0.30380874017595033	0.3362168161035516
>10	1.692981287847729	3.9476041522606296
>50	0.19306836059215282	1.6719187049849342
>100	0.1715415697369349	4.3065223477485315
>500	0.019845005126429668	1.6344882703380006
>1k	0.02926297366100646	8.288620342843105
>5k	0.004036272229104339	3.2991690161269673
>10k+	0.012108816687313019	59.691930191444555
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	218805	9.04387211066669	No Hit
CCCATGTACTCTGCGTTGATACCAC	216093	8.931776952127679	No Hit
GAGTACATGGGAAGCAGTGGTATCA	101488	4.1948058443241285	No Hit
CATGTACTCTGCGTTGATACCACTG	81157	3.3544641524890952	No Hit
GCGTTGATACCACTGCTTCCCATGT	66639	2.7543913237024635	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	54638	2.2583537139581207	No Hit
TATCAACGCAGAGTACATGGGAAGC	54404	2.248681786562056	No Hit
GTATCAACGCAGAGTACATGGGAAG	53189	2.1984621635440256	No Hit
ACTCTGCGTTGATACCACTGCTTCC	49125	2.030484757827751	No Hit
GGTATCAACGCAGAGTACATGGGAA	46436	1.9193402588191237	No Hit
GCTTCCCATGTACTCTGCGTTGATA	38510	1.5917347180447166	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	26473	1.09420911947021	No Hit
GTACTCTGCGTTGATACCACTGCTT	24638	1.0183630221549138	No Hit
GTGGTATCAACGCAGAGTACATGGG	23952	0.9900085683356803	No Hit
ATACCACTGCTTCCCATGTACTCTG	23832	0.9850486055684676	No Hit
CAGTGGTATCAACGCAGAGTACATG	22594	0.9338783230200551	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	21646	0.894694617159074	No Hit
ACATGGGAAGCAGTGGTATCAACGC	20993	0.867704153100824	No Hit
GCAGTGGTATCAACGCAGAGTACAT	20384	0.842532342057219	No Hit
CATGGGAAGCAGTGGTATCAACGCA	19532	0.8073166064100079	No Hit
TACCACTGCTTCCCATGTACTCTGC	19491	0.8056219524645435	No Hit
GTTGATACCACTGCTTCCCATGTAC	19324	0.7987193376135058	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	19322	0.7986366715673855	No Hit
GATACCACTGCTTCCCATGTACTCT	19293	0.7974380138986423	No Hit
GTACATGGTAAGCAGTGGTATCAAC	18524	0.7656529191654202	No Hit
CCATGTACTCTGCGTTGATACCACT	18387	0.7599902950061855	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	17408	0.7195252654303408	No Hit
CTTCCCATGTACTCTGCGTTGATAC	16940	0.7001814106382108	No Hit
CCACTGCTTCCCATGTACTCTGCGT	16472	0.6808375558460809	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	15891	0.6568230694481586	No Hit
TTCCCATGTACTCTGCGTTGATACC	14823	0.6126794008199645	No Hit
ACCATGTACTCTGCGTTGATACCAC	14713	0.6081327682833527	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	13954	0.5767610037807317	No Hit
TGATACCACTGCTTCCCATGTACTC	12101	0.5001709120503535	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	11852	0.48987898930838697	No Hit
ATCAACGCAGAGTACATGGGAAGCA	10221	0.4224648286973526	No Hit
GAACAAAAAAAAAAAAAAAAAAAAA	8177	0.33798012956249407	No Hit
GGTAAGCAGTGGTATCAACGCAGAG	7859	0.32483622822938013	No Hit
CCCCATGTACTCTGCGTTGATACCA	7674	0.3171896189632603	No Hit
GTACATGGGTAAGCAGTGGTATCAA	7264	0.3002430795086165	No Hit
TCCATGTACTCTGCGTTGATACCAC	7115	0.29408445907266056	No Hit
GAGTACATGGTAAGCAGTGGTATCA	6581	0.27201262475856347	No Hit
GGAAGCAGTGGTATCAACGCAGAGT	6393	0.2642420164232634	No Hit
AAAAAGTACTCTGCGTTGATACCAC	6178	0.25535541646534043	No Hit
AAACAAAAAAAAAAAAAAAAAAAAA	6025	0.24903146393714404	No Hit
GCTTACCATGTACTCTGCGTTGATA	5878	0.24295550954730832	No Hit
GTATCAACGCAGAGTACTTTTTTTT	5564	0.22997694030643476	No Hit
GTACATGGAAAGCAGTGGTATCAAC	5060	0.20914509668414089	No Hit
CTGCGTTGATACCACTGCTTCCCAT	4934	0.20393713577856742	No Hit
CTCTGCGTTGATACCACTGCTTCCC	4914	0.20311047531736529	No Hit
GCGTTGATACCACTGCTTACCATGT	4642	0.1918678930450162	No Hit
TCCCATGTACTCTGCGTTGATACCA	4625	0.1911652316529944	No Hit
TGGGAAGCAGTGGTATCAACGCAGA	4595	0.18992524096119118	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4468	0.1846759470325576	No Hit
ACTCTGCGTTGATACCACTGCTTAC	4384	0.18120397309550862	No Hit
AAAGTACTCTGCGTTGATACCACTG	4345	0.17959198519616446	No Hit
TCAACGCAGAGTACATGGGAAGCAG	4330	0.17897198985026286	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4326	0.17880665775802243	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	4188	0.17310270057572769	No Hit
ACGCAGAGTACATGGTAAGCAGTGG	3991	0.1649600950328866	No Hit
AGTGGTATCAACGCAGAGTACATGG	3783	0.1563628262363844	No Hit
CTGCTTACCATGTACTCTGCGTTGA	3701	0.1529735183454556	No Hit
GAGTACATGGAAAGCAGTGGTATCA	3627	0.1499148746390077	No Hit
GTATCAACGCAGAGTACATGGTAAG	3609	0.1491708802239258	No Hit
TATCAACGCAGAGTACATGGTAAGC	3591	0.14842688580884386	No Hit
ATGGTAAGCAGTGGTATCAACGCAG	3444	0.14235093141900815	No Hit
AAAAACAAAAAAAAAAAAAAAAAAA	3376	0.13954028585092088	No Hit
GGTATCAACGCAGAGTACATGGTAA	3322	0.13730830260567511	No Hit
TTGATACCACTGCTTCCCATGTACT	3316	0.13706030446731446	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAA	3184	0.13160434542338037	No Hit
AACGCAGAGTACATGGGAAGCAGTG	3092	0.12780170730185053	No Hit
GTACATGGAAGCAGTGGTATCAACG	3086	0.12755370916348988	No Hit
ACTGCTTCCCATGTACTCTGCGTTG	2978	0.12308974267299833	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	2942	0.12160175384283449	No Hit
ATGTACTCTGCGTTGATACCACTGC	2930	0.1211057575661132	No Hit
GCTTTCCATGTACTCTGCGTTGATA	2871	0.11866710920556689	No Hit
GAAAACAAAAAAAAAAAAAAAAAAA	2649	0.10949117808622318	No Hit
CGTTGATACCACTGCTTCCCATGTA	2562	0.10589520507999386	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	4.1333023060106896E-5	0.0
4	0.0	0.0	0.0	4.1333023060106896E-5	0.0
5	0.0	0.0	0.0	4.1333023060106896E-5	0.0
6	4.1333023060106896E-5	0.0	0.0	4.1333023060106896E-5	0.0
7	4.1333023060106896E-5	0.0	0.0	4.1333023060106896E-5	0.0
8	8.266604612021379E-5	0.0	0.0	4.1333023060106896E-5	0.0
9	8.266604612021379E-5	0.0	0.0	8.266604612021379E-5	0.0
10	8.266604612021379E-5	0.0	0.0	2.893311614207483E-4	0.0
11	8.266604612021379E-5	0.0	0.0	4.1333023060106895E-4	0.0
12	8.266604612021379E-5	0.0	0.0	6.199953459016034E-4	0.0
13	8.266604612021379E-5	0.0	0.0	6.613283689617103E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACATAA	835	0.0	17.18605	2
TCTAGAA	35	0.0021652654	16.29179	2
GTACAAA	975	0.0	16.18912	1
GTACATA	1035	0.0	16.07746	1
AGAACAA	1565	0.0	15.2421055	2
TACAAAA	1185	0.0	14.275367	2
GTACCGG	40	0.005248364	14.263005	1
TACATGA	725	0.0	14.025921	2
TACAAGA	185	0.0	13.870038	2
GGACAGC	55	1.9618933E-4	13.817323	6
GTAAAAA	470	0.0	13.757224	1
ACATAAA	1720	0.0	13.316289	3
TTGCAAC	50	0.001489691	13.312137	1
CCGGGGT	50	0.001494354	13.306618	4
GACCACT	50	0.001500669	13.299174	8
CGCAAAA	620	0.0	13.029053	2
CGCAGAA	570	0.0	12.838122	2
GAGAACA	2280	0.0	12.761636	1
TTTCAAC	90	5.4227166E-7	12.6640415	19
CATAAAA	1720	0.0	12.322989	4
>>END_MODULE