FastQCFastQC Report
Wed 25 May 2016
SRR1294996_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294996_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2419373
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC2369019.791834495962384No Hit
GTACATGGGAAGCAGTGGTATCAAC2142368.85502152830506No Hit
GAGTACATGGGAAGCAGTGGTATCA1088404.4986862298620345No Hit
CATGTACTCTGCGTTGATACCACTG901363.725593366545795No Hit
GCGTTGATACCACTGCTTCCCATGT715622.9578737962273696No Hit
ACGCAGAGTACATGGGAAGCAGTGG674922.7896483923727344No Hit
TATCAACGCAGAGTACATGGGAAGC601262.485189344511987No Hit
GTATCAACGCAGAGTACATGGGAAG557812.305597359315823No Hit
ACTCTGCGTTGATACCACTGCTTCC556062.298364080280304No Hit
GGTATCAACGCAGAGTACATGGGAA509552.1061241900277468No Hit
GCTTCCCATGTACTCTGCGTTGATA418801.731027005757277No Hit
CAGTGGTATCAACGCAGAGTACATG276241.141783429012393No Hit
ACATGGGAAGCAGTGGTATCAACGC267981.1076423519647445No Hit
GTGGTATCAACGCAGAGTACATGGG253531.0479161336428902No Hit
CATGGGAAGCAGTGGTATCAACGCA250251.034358902079175No Hit
ATACCACTGCTTCCCATGTACTCTG247711.0238603142219078No Hit
GGGAAGCAGTGGTATCAACGCAGAG247341.0223309923686839No Hit
GCAGTGGTATCAACGCAGAGTACAT234950.9711193767972115No Hit
GTACTCTGCGTTGATACCACTGCTT233140.9636380996233321No Hit
GTATCAACGCAGAGTACTTTTTTTT228620.9449555732001638No Hit
CCATGTACTCTGCGTTGATACCACT216240.8937852906517515No Hit
GATACCACTGCTTCCCATGTACTCT212000.8762600888742662No Hit
TACCACTGCTTCCCATGTACTCTGC210630.8705974647150316No Hit
CTGCTTCCCATGTACTCTGCGTTGA206170.8521629364302239No Hit
GTACTTTTTTTTTTTTTTTTTTTTT203060.8393083662585307No Hit
GCAGAGTACATGGGAAGCAGTGGTA202570.8372830481285854No Hit
TATCAACGCAGAGTACTTTTTTTTT199530.8247178091183129No Hit
GGTATCAACGCAGAGTACTTTTTTT198080.8187245207745975No Hit
CTTCCCATGTACTCTGCGTTGATAC192570.7959500250684785No Hit
GTTGATACCACTGCTTCCCATGTAC188480.7790448186368948No Hit
GTACATGGTAAGCAGTGGTATCAAC188460.7789621525907745No Hit
AAGCAGTGGTATCAACGCAGAGTAC186310.7700755526328515No Hit
CCACTGCTTCCCATGTACTCTGCGT183100.7568076522305572No Hit
ACCATGTACTCTGCGTTGATACCAC162900.6733149456491413No Hit
ATGGGAAGCAGTGGTATCAACGCAG162450.6714549596114365No Hit
TTCCCATGTACTCTGCGTTGATACC142000.5869289274535179No Hit
ACGCAGAGTACTTTTTTTTTTTTTT121250.5011629046037961No Hit
TGATACCACTGCTTCCCATGTACTC118640.4903749855851082No Hit
ATCAACGCAGAGTACATGGGAAGCA118520.48987898930838697No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA113330.46842715034019144No Hit
GGTAAGCAGTGGTATCAACGCAGAG90050.3722038726562626No Hit
GAGTACTTTTTTTTTTTTTTTTTTT88070.36401993409036143No Hit
CCCCATGTACTCTGCGTTGATACCA87790.36286260944467846No Hit
GTACATGGGTAAGCAGTGGTATCAA75440.31181632596544645No Hit
GGAAGCAGTGGTATCAACGCAGAGT74680.3086750162128783No Hit
GAGTACATGGTAAGCAGTGGTATCA72150.29821776137867123No Hit
TCCATGTACTCTGCGTTGATACCAC71510.2955724479028244No Hit
AAAAAGTACTCTGCGTTGATACCAC63360.2618860341088373No Hit
GCTTACCATGTACTCTGCGTTGATA61330.25349543042763556No Hit
CTGCGTTGATACCACTGCTTCCCAT56830.23489557005058748No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA55080.22766229101506877No Hit
TGGGAAGCAGTGGTATCAACGCAGA54390.2248103124239214No Hit
CTCTGCGTTGATACCACTGCTTCCC53000.21906502221856655No Hit
TCAACGCAGAGTACATGGGAAGCAG52310.21621304362741917No Hit
ACTCTGCGTTGATACCACTGCTTAC50120.20716111157725575No Hit
TCCCATGTACTCTGCGTTGATACCA49670.20530112553955093No Hit
GTACATGGAAAGCAGTGGTATCAAC49630.20513579344731053No Hit
GCGTTGATACCACTGCTTACCATGT48570.2007544930029392No Hit
ACGCAGAGTACATGGTAAGCAGTGG46920.19393454419802156No Hit
AAAGTACTCTGCGTTGATACCACTG45690.1888505823616284No Hit
AGTGGTATCAACGCAGAGTACATGG43900.18145197123386927No Hit
CTGCTTACCATGTACTCTGCGTTGA41000.16946539454643827No Hit
TATCAACGCAGAGTACATGGTAAGC40600.167812073624034No Hit
ATGGTAAGCAGTGGTATCAACGCAG40520.16748140943955314No Hit
GAGTACATGGAAAGCAGTGGTATCA40460.1672334113011925No Hit
GTGGTATCAACGCAGAGTACTTTTT39860.1647534299175861No Hit
GCAGAGTACTTTTTTTTTTTTTTTT37200.15375884578359764No Hit
GTATCAACGCAGAGTACATGGTAAG37070.15322151648381627No Hit
AACGCAGAGTACATGGGAAGCAGTG36820.1521881909073136No Hit
GAACAAAAAAAAAAAAAAAAAAAAA34330.14189626816534698No Hit
GGTATCAACGCAGAGTACATGGTAA34310.14181360211922675No Hit
ACTGCTTCCCATGTACTCTGCGTTG32670.1350349863373692No Hit
CAACGCAGAGTACATGGGAAGCAGT31850.13164567844644046No Hit
TTGATACCACTGCTTCCCATGTACT30970.12800837241715105No Hit
GTACATGGAAGCAGTGGTATCAACG30900.1277190412557303No Hit
GCTTTCCATGTACTCTGCGTTGATA30010.1240404022033808No Hit
ATCAACGCAGAGTACTTTTTTTTTT29910.12362707197277971No Hit
CGCAGAGTACATGGGAAGCAGTGGT29100.12027909710491107No Hit
CGTTGATACCACTGCTTCCCATGTA29000.11986576687431001No Hit
ATGTACTCTGCGTTGATACCACTGC27980.11564979852217909No Hit
ACGCAGAGTACATGGAAAGCAGTGG26730.11048317063966573No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAA4050.017.8514731
GGTGTCC350.002171616.284678
GGACGCA1750.016.2846688
GGGACGC1800.015.8323177
GCAACCC508.723448E-515.198713
TAGTACA456.7686074E-414.7765254
TACAAAA4650.014.7087342
ATACGAC653.3769738E-614.61414419
GTACAAG2150.014.6012821
CGCAAAA3600.014.5129582
GAACAAA10700.014.3138881
GCTTTCA400.005232698714.2694341
GTCAACA400.005282475614.2490852
TGGGACG1950.014.12700756
GATACGA759.688374E-713.93215218
TTACTCT1500.013.9321524
TACAAGA1301.8189894E-1213.8837242
CATTTAC1257.2759576E-1213.6986561
TTAGACA500.001500927813.2988714
TACAAGG1301.4551915E-1113.1530022
Produced by FastQC (version 0.11.5)