##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294996_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2419373
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.81552865143159	33.0	33.0	33.0	27.0	33.0
2	31.694725038264046	33.0	33.0	33.0	27.0	33.0
3	31.977902952541836	33.0	33.0	33.0	27.0	33.0
4	35.616331173407325	37.0	37.0	37.0	33.0	37.0
5	35.967420897893795	37.0	37.0	37.0	33.0	37.0
6	35.439236942794686	37.0	37.0	37.0	33.0	37.0
7	35.43875830638765	37.0	37.0	37.0	33.0	37.0
8	35.40988016316624	37.0	37.0	37.0	33.0	37.0
9	35.29613871031875	37.0	37.0	37.0	33.0	37.0
10	35.409044409439964	37.0	37.0	37.0	33.0	37.0
11	35.35723139838297	37.0	37.0	37.0	33.0	37.0
12	35.407784165566866	37.0	37.0	37.0	33.0	37.0
13	35.19178812031051	37.0	37.0	37.0	33.0	37.0
14	36.92011649299219	40.0	37.0	40.0	33.0	40.0
15	36.82095278404777	40.0	37.0	40.0	33.0	40.0
16	36.72867805005677	40.0	37.0	40.0	33.0	40.0
17	36.82791863842409	40.0	37.0	40.0	33.0	40.0
18	36.81536414599981	40.0	37.0	40.0	33.0	40.0
19	36.50079834734041	40.0	37.0	40.0	33.0	40.0
20	36.655044096135654	40.0	37.0	40.0	33.0	40.0
21	36.648415519227505	40.0	37.0	40.0	33.0	40.0
22	36.77254974739323	40.0	37.0	40.0	33.0	40.0
23	36.73935023661089	40.0	37.0	40.0	33.0	40.0
24	36.76770097045805	40.0	37.0	40.0	33.0	40.0
25	35.76924641219026	37.0	37.0	40.0	27.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2684309428608529
1101	2	0.2737514912685839
1101	3	0.19121857634052475
1101	4	0.21776003713436154
1101	5	0.24580514275688614
1101	6	0.29584616984421785
1101	7	0.2624503473847497
1101	8	0.28005365649851655
1101	9	0.23263392325060295
1101	10	0.32026697288208084
1101	11	0.3648474981508869
1101	12	0.33789386974147106
1101	13	0.2921134919684576
1101	14	0.6212723849588144
1101	15	0.6588008711007163
1101	16	0.6360180095090655
1101	17	0.6777825926110808
1101	18	0.752324205727426
1101	19	0.6746216253130868
1101	20	0.6777386556781195
1101	21	0.6769420540075402
1101	22	0.6627991534785025
1101	23	0.7126122614063988
1101	24	0.818949000672319
1101	25	0.9022570193965933
1102	1	0.2811522211271722
1102	2	0.26848299099428274
1102	3	0.21004898935647986
1102	4	0.28334926844695474
1102	5	0.20436635531837055
1102	6	0.29417753257887114
1102	7	0.22207119940280506
1102	8	0.3193184556053623
1102	9	0.3608470350068913
1102	10	0.4247842977822458
1102	11	0.4811572123518957
1102	12	0.2928274463170055
1102	13	0.459164100522905
1102	14	0.6907311783099601
1102	15	0.818152425626181
1102	16	0.7625965309928873
1102	17	0.725482680597878
1102	18	0.7547848747583643
1102	19	0.7125414427208199
1102	20	0.7256847687412389
1102	21	0.8030172321101148
1102	22	0.7156456075636655
1102	23	0.696556552829918
1102	24	0.6322413147686277
1102	25	0.6713994183092282
1103	1	0.20567239149996652
1103	2	0.23752290661108688
1103	3	0.12941642045174717
1103	4	0.14521511159698974
1103	5	0.27007268528303996
1103	6	0.1806925694405379
1103	7	0.2509674620583979
1103	8	0.2153396640144436
1103	9	0.21553144706798832
1103	10	0.2508315810268087
1103	11	0.2722632100183944
1103	12	0.2848741306492357
1103	13	0.2657257198157055
1103	14	0.4236578970613962
1103	15	0.4588153279560103
1103	16	0.6262194378725638
1103	17	0.5931415523985919
1103	18	0.5872928173241192
1103	19	0.4828040416839414
1103	20	0.523431699802309
1103	21	0.4429454080634514
1103	22	0.5101011981166224
1103	23	0.5990729825562582
1103	24	0.47093974893983415
1103	25	0.6010070171453847
1104	1	0.24224769249834566
1104	2	0.19309066265961405
1104	3	0.17267184832255822
1104	4	0.16708485221673186
1104	5	0.19103490912988264
1104	6	0.18608093540582615
1104	7	0.2636033016173087
1104	8	0.1103567224930444
1104	9	0.15512425204541103
1104	10	0.2781349040032097
1104	11	0.33365017284766196
1104	12	0.186023721702675
1104	13	0.2526477115453858
1104	14	0.3704127404508526
1104	15	0.4104180824433783
1104	16	0.5445274595459679
1104	17	0.47086476906412145
1104	18	0.506974465371151
1104	19	0.4808235637419287
1104	20	0.5719541233247298
1104	21	0.4115895065152486
1104	22	0.39468969087818806
1104	23	0.5607599442432161
1104	24	0.6165374810760227
1104	25	0.7270760278297743
1105	1	-0.054171226420574925
1105	2	0.014190512509461684
1105	3	-0.03421253606182617
1105	4	-0.04517866129678083
1105	5	0.09990127549625072
1105	6	0.06996231678721188
1105	7	0.09985705537106782
1105	8	0.08331468045099655
1105	9	0.07924587366702696
1105	10	0.14546598508429298
1105	11	0.1035938665413596
1105	12	0.10154549347444686
1105	13	0.14080837220604536
1105	14	0.12639247018058342
1105	15	0.11286552989082566
1105	16	0.25680598807449684
1105	17	0.217430084379437
1105	18	0.18175299389967847
1105	19	0.1540292694476335
1105	20	0.29342559479620434
1105	21	0.1481135305392698
1105	22	0.2995432944817935
1105	23	0.24949117547444644
1105	24	0.27587682041536254
1105	25	0.23643788969163637
1106	1	-0.3151695964287242
1106	2	-0.09478530927809459
1106	3	-0.037167575453295854
1106	4	0.03002305019465723
1106	5	0.09663993346962485
1106	6	0.15312178432320422
1106	7	0.14721307275764417
1106	8	0.048890610904678056
1106	9	0.13014848165398973
1106	10	-0.04978462616265489
1106	11	0.08227968561226362
1106	12	0.16000786782923626
1106	13	-0.06337934852201244
1106	14	-0.0971120122970035
1106	15	-0.06265538833791595
1106	16	-0.0015246286057504221
1106	17	-0.7162903720182854
1106	18	-0.4737760742883168
1106	19	-0.4712912956162114
1106	20	-0.4921965187598971
1106	21	-0.7664884797840088
1106	22	-0.4553792809053263
1106	23	-0.14345435563122777
1106	24	-0.7499436088158191
1106	25	0.02951110349223285
1107	1	0.09482877357942243
1107	2	0.0638794013983528
1107	3	0.011043019493733652
1107	4	-0.2631453279634499
1107	5	-0.018120946725971976
1107	6	0.17731787234276197
1107	7	0.07073483314884044
1107	8	0.18273690267321996
1107	9	0.040879207000365625
1107	10	0.1099659850842869
1107	11	-0.0050394667919775316
1107	12	-0.12201006208110954
1107	13	0.04109726109494005
1107	14	-0.1850075298194156
1107	15	0.0026099743352716587
1107	16	0.18345043251894566
1107	17	0.2560967510461012
1107	18	0.30423077167745305
1107	19	0.2005848250031903
1107	20	0.00998115035175573
1107	21	0.2207579749837123
1107	22	0.27727662781512663
1107	23	0.45910228658556207
1107	24	0.37892126485981237
1107	25	0.2936490008027519
1108	1	-0.01338554893474253
1108	2	0.0231000093324063
1108	3	-0.05775409539550225
1108	4	0.004727315004089405
1108	5	0.009026296962844071
1108	6	0.05175950036955612
1108	7	0.09150268477171863
1108	8	0.0054390149864644854
1108	9	9.85956098766394E-4
1108	10	0.13257701032900826
1108	11	0.03505574186335991
1108	12	0.1122959657395981
1108	13	0.0616318309043109
1108	14	0.08824460825373848
1108	15	0.07816649159441624
1108	16	0.028367897743052595
1108	17	0.006507879330492017
1108	18	-0.02431904800312168
1108	19	-0.017929342951475746
1108	20	0.005923448136407217
1108	21	-0.003925796268219983
1108	22	0.09986786944315185
1108	23	0.12103118921307043
1108	24	-0.031092954614685198
1108	25	-0.05909576993398247
1109	1	0.04510834347189885
1109	2	-0.04578081365541209
1109	3	-0.07955482996863594
1109	4	-0.07206575807097693
1109	5	0.04538873069338223
1109	6	-0.019611159915299936
1109	7	0.05042515572948503
1109	8	-0.028893204853659427
1109	9	-0.014716491924367858
1109	10	-0.08758240201248668
1109	11	0.024448705251032266
1109	12	-0.11951543842519641
1109	13	-0.034894136754523686
1109	14	-0.041747314765650856
1109	15	-0.001200778352902887
1109	16	0.07366978735765173
1109	17	0.09553761126115745
1109	18	0.151869481354872
1109	19	-0.0164904438140141
1109	20	0.021120935297993526
1109	21	0.07755797498371209
1109	22	0.14304867082588402
1109	23	0.2777259424995364
1109	24	0.1945685766877645
1109	25	0.06482319435113482
1110	1	0.07000935021068244
1110	2	-0.05993526729158205
1110	3	0.045916868760293994
1110	4	-0.008811691139982258
1110	5	0.021920327932598127
1110	6	0.0842912663741231
1110	7	0.08914090295157706
1110	8	0.07436340747847936
1110	9	0.20099979374796106
1110	10	0.08074797800285438
1110	11	0.10768971884282763
1110	12	0.05403242653800788
1110	13	0.0332607411151713
1110	14	0.03996606653870316
1110	15	0.04292074823511882
1110	16	-0.06774552094590547
1110	17	0.043024419230221156
1110	18	-0.05288323945051587
1110	19	-0.026725240753009416
1110	20	0.03442626921872716
1110	21	0.14785640695134106
1110	22	0.10162220191730142
1110	23	0.181048669994766
1110	24	0.14418823501661393
1110	25	-0.05387108012795494
1111	1	-0.10208255523243182
1111	2	-0.14367769480863046
1111	3	-0.11433331810988534
1111	4	-0.08836693057585876
1111	5	-0.030322705078148715
1111	6	-0.030288457725056617
1111	7	-0.1391153552460338
1111	8	-0.12214207245865083
1111	9	-0.3166351863681385
1111	10	-0.17285039271023095
1111	11	-0.02196064237600126
1111	12	-0.055441863136124425
1111	13	-0.5836197446825082
1111	14	-0.36364646476038587
1111	15	-0.26175998296189107
1111	16	-0.17093369207286457
1111	17	-0.1531803150176998
1111	18	-0.12028648529315689
1111	19	-0.20772364020655942
1111	20	-0.14885230857816367
1111	21	-0.1551875663375597
1111	22	-0.1989168914011472
1111	23	-0.16321270964654389
1111	24	-0.15114193534114406
1111	25	-0.2651607454921532
1112	1	-0.1311767792541545
1112	2	-0.12102969544959663
1112	3	-0.14725611491750712
1112	4	-0.1668751336142762
1112	5	-0.08927587078280652
1112	6	-0.11366145151809093
1112	7	-0.19935316293150862
1112	8	-0.21887433364884146
1112	9	-0.23995345672656754
1112	10	-0.6290403843424599
1112	11	-1.3578380295879882
1112	12	-0.5377761897962827
1112	13	-0.28722989879400984
1112	14	-1.904170162515804
1112	15	-0.9072176265139831
1112	16	-0.5493938594947778
1112	17	-0.3110200217776793
1112	18	-0.19475756737202943
1112	19	-0.24481906198031567
1112	20	-0.34653167016106323
1112	21	-0.3757296291319179
1112	22	-0.2545494705026883
1112	23	-0.23563689355489714
1112	24	-0.3222207896886147
1112	25	-0.42831944603703676
1113	1	-0.11631532570126524
1113	2	-0.06925005872337664
1113	3	-0.15975353413839244
1113	4	-0.043139889658178276
1113	5	-0.07004088981347678
1113	6	-0.11460662374449981
1113	7	-0.13994934991968933
1113	8	-0.07252728197621394
1113	9	-0.0936398665906566
1113	10	-0.1531224664215216
1113	11	-0.036008338817900665
1113	12	-0.1420426286766414
1113	13	-0.20962492687439038
1113	14	-0.35673099831993227
1113	15	-0.8217069018010008
1113	16	-0.8548095627383461
1113	17	-0.5359807131859782
1113	18	-0.6736545496410145
1113	19	-0.5161803319014027
1113	20	-0.5297734935499108
1113	21	-0.3134593359008022
1113	22	-0.2560072548818937
1113	23	-0.24637206326820404
1113	24	-0.35294922759138103
1113	25	-0.49475773384273225
1114	1	-0.1903826371278825
1114	2	-0.3136693285742922
1114	3	-0.2169229046954051
1114	4	-0.160386750394089
1114	5	-0.21858706630393243
1114	6	-0.2519088568483241
1114	7	-0.6590855654443573
1114	8	-0.27852327708449565
1114	9	-0.28009273516454414
1114	10	-0.3793610958066793
1114	11	-0.37023086968373065
1114	12	-0.3203250288650139
1114	13	-0.285693516552584
1114	14	-0.5589748608080853
1114	15	-0.5577085876029884
1114	16	-0.7501505053474133
1114	17	-1.1671732763083327
1114	18	-0.9848215143717809
1114	19	-1.201433150768601
1114	20	-2.1320759031847842
1114	21	-1.7176120132929569
1114	22	-1.328995197116484
1114	23	-0.812802363668446
1114	24	-1.2408373908650816
1114	25	-1.1908068022453193
1115	1	-0.2309519405939291
1115	2	-0.4791889641828959
1115	3	-0.2330014039764592
1115	4	-0.21923128045676776
1115	5	-0.20549461354994492
1115	6	-0.46071855993661615
1115	7	-0.4381119813863137
1115	8	-1.303591617068797
1115	9	-0.5869083657540841
1115	10	-0.5487447275159951
1115	11	-0.4577168932509963
1115	12	-0.43341858811540845
1115	13	-0.49824909725019495
1115	14	-0.8764335483816339
1115	15	-0.784297308974466
1115	16	-0.908393267339477
1115	17	-0.8029883519899244
1115	18	-0.7450456794787499
1115	19	-0.9780170312183003
1115	20	-0.8835774464706461
1115	21	-1.4226496701319036
1115	22	-1.1466404399841466
1115	23	-0.8768253522377876
1115	24	-1.2214146168453937
1115	25	-1.1010364168470872
1116	1	-0.16437058846204167
1116	2	-0.3870800882348213
1116	3	-0.2509735674281188
1116	4	-0.17145856560300388
1116	5	-0.7725362577307564
1116	6	-0.47225724727446305
1116	7	-0.5601283247458966
1116	8	-0.5362174832916935
1116	9	-0.535774062537115
1116	10	-0.8045699000831732
1116	11	-0.5320161813055293
1116	12	-0.6278537622406049
1116	13	-0.6339470770230875
1116	14	-0.8426107196120824
1116	15	-1.3865855599550017
1116	16	-1.329778275615027
1116	17	-1.2080882569283844
1116	18	-0.9135433910019728
1116	19	-1.1737245848851714
1116	20	-0.9823984349751953
1116	21	-1.0944037475043231
1116	22	-1.0946991297606274
1116	23	-0.8695452413251274
1116	24	-0.9960503459854877
1116	25	-1.3040179848431848
1201	1	0.14753616159571337
1201	2	0.3048478714390761
1201	3	0.18997449127964927
1201	4	0.2297966150266575
1201	5	0.26177154891102106
1201	6	0.15388610969005612
1201	7	0.26764792215407596
1201	8	0.25119717027066457
1201	9	0.25042242980431695
1201	10	0.2609211915997065
1201	11	0.2819431975477542
1201	12	0.28787475467281354
1201	13	0.4020736427121534
1201	14	0.6661510507506847
1201	15	0.7038879266331151
1201	16	0.7234522940663553
1201	17	0.7671101309181196
1201	18	0.7090959258368486
1201	19	0.7728803456547269
1201	20	0.692422104394808
1201	21	0.6587447114584108
1201	22	0.6307649349704505
1201	23	0.6136898374872501
1201	24	0.7036226028470125
1201	25	0.7812008332634832
1202	1	0.1760528551335696
1202	2	0.24361411961339385
1202	3	0.12130022280205566
1202	4	0.25345232543143226
1202	5	0.23618164992722512
1202	6	0.2445917327005276
1202	7	0.34865474082339887
1202	8	0.27637552548529953
1202	9	0.36681127085879695
1202	10	0.4126457311893077
1202	11	0.41970898483333485
1202	12	0.4067900917946332
1202	13	0.39359704951579033
1202	14	0.7289593099574603
1202	15	0.7205274199979002
1202	16	0.641328332115016
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2210	13	0.05749296001966542
2210	14	0.23205913684724777
2210	15	0.20267018938903192
2210	16	0.05379881961571442
2210	17	0.2697311596482521
2210	18	0.21638561038713533
2210	19	0.10763858844404695
2210	20	0.1803467417496094
2210	21	0.2628482975643607
2210	22	0.16111318695491406
2210	23	0.2359194908866371
2210	24	0.23869760894583436
2210	25	0.22121029112533108
2211	1	0.018381125107058693
2211	2	6.875944847095639E-5
2211	3	-0.015494433304073851
2211	4	-0.08493921742448407
2211	5	0.016499856569595295
2211	6	0.061547738460170365
2211	7	0.05002669720651909
2211	8	0.033799861821869115
2211	9	-0.10267396842016296
2211	10	0.05608133008944094
2211	11	0.11208253801406443
2211	12	0.01624397156118107
2211	13	-0.04124163351543331
2211	14	0.04780167031790228
2211	15	-0.12828134046047523
2211	16	-0.05108221423697756
2211	17	-3.6943501638120324E-4
2211	18	-0.04714018609803361
2211	19	-0.02952090791819728
2211	20	0.029765565044513664
2211	21	-0.009424517292288215
2211	22	0.012378859191713332
2211	23	0.16177032640705136
2211	24	0.13946475953883208
2211	25	-0.04557406819827747
2212	1	0.01700464270007984
2212	2	-0.013525915575062442
2212	3	-0.06114675555739524
2212	4	-0.02154369197162964
2212	5	0.014580893764822633
2212	6	0.1129105103795709
2212	7	0.001821949713260551
2212	8	0.048215839278540784
2212	9	-0.053335925923974514
2212	10	0.029008929869569045
2212	11	0.04121656592786138
2212	12	0.0053355411908739825
2212	13	-0.024034845244528924
2212	14	-0.11095926806072498
2212	15	-0.07493440194284773
2212	16	0.05833632208949524
2212	17	0.07616219071890384
2212	18	0.05543567965291629
2212	19	0.062417135841634774
2212	20	0.08032061865441875
2212	21	0.026024232652424928
2212	22	0.0862713108417168
2212	23	0.11128224568576428
2212	24	0.04254007876573951
2212	25	0.15096924620151952
2213	1	-0.04188255018453191
2213	2	-0.07487913129702406
2213	3	-0.02059079230850358
2213	4	0.012991195162548763
2213	5	0.04229627815441006
2213	6	0.015302561337982468
2213	7	0.05566114893832008
2213	8	0.0295719903925189
2213	9	-0.21807071325481786
2213	10	-0.13184262735590124
2213	11	-0.14756642053998092
2213	12	-0.12641835554203595
2213	13	-0.008588225348447054
2213	14	-0.14739540860729505
2213	15	-0.22151378962006874
2213	16	-0.24365387370114888
2213	17	-0.11526529042120615
2213	18	-0.21737114219814657
2213	19	-0.1500403743588521
2213	20	-0.07306829143452376
2213	21	-0.09464298195408816
2213	22	-0.02651731157881443
2213	23	-0.040597872904072574
2213	24	0.03194008463652409
2213	25	-0.15952037718768253
2214	1	0.05101509917550828
2214	2	0.027819650885167135
2214	3	0.016667905014017492
2214	4	0.011354972052338042
2214	5	0.047548783259813376
2214	6	0.13394278944366533
2214	7	0.02666017658797415
2214	8	0.0907303339064427
2214	9	0.10814489466813626
2214	10	0.021357742545212943
2214	11	-0.0023073651024105857
2214	12	-0.02086621240481179
2214	13	-0.011625176928120595
2214	14	-0.1212918816274069
2214	15	-0.1895678876574678
2214	16	-0.18392957695523648
2214	17	-0.08230148043976726
2214	18	-0.059454306484553854
2214	19	-0.1142530085669975
2214	20	-0.13062961863608535
2214	21	-0.07135723107976588
2214	22	-0.06575119470184632
2214	23	0.05097849059630022
2214	24	-0.022602310065188647
2214	25	-0.1104673421626714
2215	1	-0.0523442065119859
2215	2	-0.11980850626297013
2215	3	-0.031321594341577
2215	4	-0.07852690670696205
2215	5	-0.07934279195023919
2215	6	-0.10855021968605172
2215	7	-0.2261875001070237
2215	8	-0.2604643367721238
2215	9	-0.1987557143723251
2215	10	-0.22612418456478878
2215	11	-0.1641722268818384
2215	12	-0.20315407013750786
2215	13	-0.27138996487144595
2215	14	-0.5601212024509152
2215	15	-0.5618594036180937
2215	16	-0.601304761531722
2215	17	-0.4574169215853985
2215	18	-0.3942125469374673
2215	19	-0.5615163447233584
2215	20	-0.5890368990711252
2215	21	-0.4704968704729424
2215	22	-0.3424839733158649
2215	23	-0.3433924070390404
2215	24	-0.3827273833701099
2215	25	-0.5213081918535352
2216	1	-0.33866359253698164
2216	2	-0.5217690834501312
2216	3	-0.23989719596004022
2216	4	-0.2721799400691651
2216	5	-0.8578138553518784
2216	6	-0.6317629925723693
2216	7	-0.7771158062373473
2216	8	-0.5496262376817356
2216	9	-0.6609236605498197
2216	10	-0.6394253192635375
2216	11	-0.3170600511522039
2216	12	-0.5236760312355742
2216	13	-0.6529536727964071
2216	14	-1.0853981986561223
2216	15	-1.002788646140587
2216	16	-1.1087856056117218
2216	17	-0.9180577866583093
2216	18	-0.9892142876110839
2216	19	-0.9167977991927359
2216	20	-0.9217020241037943
2216	21	-0.8542625318751575
2216	22	-0.7193910618694446
2216	23	-0.8574960238848703
2216	24	-0.7450034658229754
2216	25	-0.9385234242146865
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	48.0
9	100.0
10	168.0
11	350.0
12	721.0
13	1518.0
14	3306.0
15	5929.0
16	8505.0
17	9792.0
18	10049.0
19	10241.0
20	10513.0
21	11118.0
22	11909.0
23	12447.0
24	13559.0
25	14657.0
26	15311.0
27	16456.0
28	18338.0
29	21931.0
30	27625.0
31	37782.0
32	52377.0
33	75747.0
34	113298.0
35	188633.0
36	376259.0
37	1350681.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.96755860096053	17.69249043528358	9.881829604846013	25.458121358909878
2	9.18759638527574	25.563142146555634	30.95282283048137	34.29643863768726
3	24.376811678067003	31.86164349192952	25.3818241337735	18.379720696229974
4	13.749471453967619	23.70589404775535	25.339333786067712	37.20530071220932
5	9.10398925990743	41.945054572599375	36.39347162414966	12.55748454334353
6	35.00328390868213	24.891159817026974	19.486495054710456	20.619061219580445
7	19.50410292253406	34.843779772693175	24.200195670531166	21.4519216342416
8	31.02234071134809	32.12048690764048	22.998160662987992	13.859011718023437
9	29.25497639264388	15.8839500978146	19.403663676498002	35.45740983304352
10	19.001121364915623	32.5111919493191	30.89176410582411	17.595922579941167
11	24.29782427099914	27.822828476634236	14.756839891988543	33.12250736037809
12	26.166118246339032	35.884586626369725	25.886665677429647	12.062629449861596
13	33.78776743797704	18.553898450242336	20.07848402006162	27.579850091719
14	22.56518527734252	29.985702907323507	24.51457464392634	22.934537171407634
15	43.20917030982821	15.265980070042943	20.552597718499793	20.972251901629058
16	14.664708583587565	24.500480083062843	40.788295149197744	20.046516184151844
17	29.643258811270524	29.682938513408224	28.174489836829625	12.499312838491626
18	30.77880095380084	18.916347334619342	31.779473442086026	18.52537826949379
19	23.577389679061476	33.30222334464343	32.39942745496457	10.720959521330526
20	24.778733994303483	23.538867301569454	29.692321109642872	21.99007759448419
21	15.05356966453705	30.643476636302054	34.83171052995962	19.471243169201276
22	20.86887801095573	29.221000647688474	17.853964642905414	32.05615669845038
23	12.576192261383422	29.047649948974385	30.50802831973408	27.868129469908116
24	20.08440266103982	41.302041246530585	21.667303888846405	16.946252203583185
25	21.006932030062632	21.870098113170116	18.917072319867863	38.20589753689939
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	14154.0
1	14154.0
2	14328.0
3	14502.0
4	14502.0
5	14502.0
6	24185.5
7	33869.0
8	33869.0
9	33869.0
10	26932.5
11	19996.0
12	19996.0
13	19996.0
14	15086.0
15	10176.0
16	10176.0
17	10176.0
18	12073.0
19	13970.0
20	13970.0
21	13970.0
22	20264.5
23	26559.0
24	26559.0
25	26559.0
26	34445.5
27	42332.0
28	42332.0
29	42332.0
30	42963.0
31	43594.0
32	43594.0
33	43594.0
34	44579.0
35	45564.0
36	45564.0
37	45564.0
38	44475.5
39	43387.0
40	43387.0
41	43387.0
42	97538.0
43	151689.0
44	151689.0
45	151689.0
46	598500.0
47	1045311.0
48	1045311.0
49	1045311.0
50	863424.0
51	681537.0
52	681537.0
53	681537.0
54	426808.0
55	172079.0
56	172079.0
57	172079.0
58	99788.5
59	27498.0
60	27498.0
61	27498.0
62	21758.0
63	16018.0
64	16018.0
65	16018.0
66	12652.0
67	9286.0
68	9286.0
69	9286.0
70	6964.5
71	4643.0
72	4643.0
73	4643.0
74	3359.5
75	2076.0
76	2076.0
77	2076.0
78	1403.5
79	731.0
80	731.0
81	731.0
82	516.0
83	301.0
84	301.0
85	301.0
86	193.0
87	85.0
88	85.0
89	85.0
90	49.0
91	13.0
92	13.0
93	13.0
94	8.0
95	3.0
96	3.0
97	3.0
98	1.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.13230700681540217
2	0.0019013190607649172
3	0.0
4	0.0
5	0.0011159916226228862
6	0.0
7	0.0
8	9.506595303824586E-4
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0011159916226228862
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	7.439944150819241E-4
25	9.919925534425653E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2419373.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.715329789766562
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.56066121214828	6.498207518069281
2	11.913639128969443	2.076625880104719
3	4.960720609265054	1.2970294831390996
4	2.4938917926314814	0.8694035773110195
5	1.4643110331507214	0.6380976784351168
6	0.9154760383244966	0.47872053531761766
7	0.657745152741417	0.40127261466332814
8	0.43403595954260543	0.3026213222425267
9	0.3456071189813483	0.2710872017652188
>10	1.816804458214093	2.976810793970021
>50	0.18835099937814154	1.1455966962407262
>100	0.16932564590523014	3.020684297833408
>500	0.01902535347249777	1.1882104393179196
>1k	0.03424563625049599	7.091540353609886
>5k	0.007134507552186664	4.170633565106587
>10k+	0.01902535347249777	67.57345804287354
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCAC	236901	9.791834495962384	No Hit
GTACATGGGAAGCAGTGGTATCAAC	214236	8.85502152830506	No Hit
GAGTACATGGGAAGCAGTGGTATCA	108840	4.4986862298620345	No Hit
CATGTACTCTGCGTTGATACCACTG	90136	3.725593366545795	No Hit
GCGTTGATACCACTGCTTCCCATGT	71562	2.9578737962273696	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	67492	2.7896483923727344	No Hit
TATCAACGCAGAGTACATGGGAAGC	60126	2.485189344511987	No Hit
GTATCAACGCAGAGTACATGGGAAG	55781	2.305597359315823	No Hit
ACTCTGCGTTGATACCACTGCTTCC	55606	2.298364080280304	No Hit
GGTATCAACGCAGAGTACATGGGAA	50955	2.1061241900277468	No Hit
GCTTCCCATGTACTCTGCGTTGATA	41880	1.731027005757277	No Hit
CAGTGGTATCAACGCAGAGTACATG	27624	1.141783429012393	No Hit
ACATGGGAAGCAGTGGTATCAACGC	26798	1.1076423519647445	No Hit
GTGGTATCAACGCAGAGTACATGGG	25353	1.0479161336428902	No Hit
CATGGGAAGCAGTGGTATCAACGCA	25025	1.034358902079175	No Hit
ATACCACTGCTTCCCATGTACTCTG	24771	1.0238603142219078	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	24734	1.0223309923686839	No Hit
GCAGTGGTATCAACGCAGAGTACAT	23495	0.9711193767972115	No Hit
GTACTCTGCGTTGATACCACTGCTT	23314	0.9636380996233321	No Hit
GTATCAACGCAGAGTACTTTTTTTT	22862	0.9449555732001638	No Hit
CCATGTACTCTGCGTTGATACCACT	21624	0.8937852906517515	No Hit
GATACCACTGCTTCCCATGTACTCT	21200	0.8762600888742662	No Hit
TACCACTGCTTCCCATGTACTCTGC	21063	0.8705974647150316	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	20617	0.8521629364302239	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	20306	0.8393083662585307	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	20257	0.8372830481285854	No Hit
TATCAACGCAGAGTACTTTTTTTTT	19953	0.8247178091183129	No Hit
GGTATCAACGCAGAGTACTTTTTTT	19808	0.8187245207745975	No Hit
CTTCCCATGTACTCTGCGTTGATAC	19257	0.7959500250684785	No Hit
GTTGATACCACTGCTTCCCATGTAC	18848	0.7790448186368948	No Hit
GTACATGGTAAGCAGTGGTATCAAC	18846	0.7789621525907745	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	18631	0.7700755526328515	No Hit
CCACTGCTTCCCATGTACTCTGCGT	18310	0.7568076522305572	No Hit
ACCATGTACTCTGCGTTGATACCAC	16290	0.6733149456491413	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	16245	0.6714549596114365	No Hit
TTCCCATGTACTCTGCGTTGATACC	14200	0.5869289274535179	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	12125	0.5011629046037961	No Hit
TGATACCACTGCTTCCCATGTACTC	11864	0.4903749855851082	No Hit
ATCAACGCAGAGTACATGGGAAGCA	11852	0.48987898930838697	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	11333	0.46842715034019144	No Hit
GGTAAGCAGTGGTATCAACGCAGAG	9005	0.3722038726562626	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	8807	0.36401993409036143	No Hit
CCCCATGTACTCTGCGTTGATACCA	8779	0.36286260944467846	No Hit
GTACATGGGTAAGCAGTGGTATCAA	7544	0.31181632596544645	No Hit
GGAAGCAGTGGTATCAACGCAGAGT	7468	0.3086750162128783	No Hit
GAGTACATGGTAAGCAGTGGTATCA	7215	0.29821776137867123	No Hit
TCCATGTACTCTGCGTTGATACCAC	7151	0.2955724479028244	No Hit
AAAAAGTACTCTGCGTTGATACCAC	6336	0.2618860341088373	No Hit
GCTTACCATGTACTCTGCGTTGATA	6133	0.25349543042763556	No Hit
CTGCGTTGATACCACTGCTTCCCAT	5683	0.23489557005058748	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	5508	0.22766229101506877	No Hit
TGGGAAGCAGTGGTATCAACGCAGA	5439	0.2248103124239214	No Hit
CTCTGCGTTGATACCACTGCTTCCC	5300	0.21906502221856655	No Hit
TCAACGCAGAGTACATGGGAAGCAG	5231	0.21621304362741917	No Hit
ACTCTGCGTTGATACCACTGCTTAC	5012	0.20716111157725575	No Hit
TCCCATGTACTCTGCGTTGATACCA	4967	0.20530112553955093	No Hit
GTACATGGAAAGCAGTGGTATCAAC	4963	0.20513579344731053	No Hit
GCGTTGATACCACTGCTTACCATGT	4857	0.2007544930029392	No Hit
ACGCAGAGTACATGGTAAGCAGTGG	4692	0.19393454419802156	No Hit
AAAGTACTCTGCGTTGATACCACTG	4569	0.1888505823616284	No Hit
AGTGGTATCAACGCAGAGTACATGG	4390	0.18145197123386927	No Hit
CTGCTTACCATGTACTCTGCGTTGA	4100	0.16946539454643827	No Hit
TATCAACGCAGAGTACATGGTAAGC	4060	0.167812073624034	No Hit
ATGGTAAGCAGTGGTATCAACGCAG	4052	0.16748140943955314	No Hit
GAGTACATGGAAAGCAGTGGTATCA	4046	0.1672334113011925	No Hit
GTGGTATCAACGCAGAGTACTTTTT	3986	0.1647534299175861	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	3720	0.15375884578359764	No Hit
GTATCAACGCAGAGTACATGGTAAG	3707	0.15322151648381627	No Hit
AACGCAGAGTACATGGGAAGCAGTG	3682	0.1521881909073136	No Hit
GAACAAAAAAAAAAAAAAAAAAAAA	3433	0.14189626816534698	No Hit
GGTATCAACGCAGAGTACATGGTAA	3431	0.14181360211922675	No Hit
ACTGCTTCCCATGTACTCTGCGTTG	3267	0.1350349863373692	No Hit
CAACGCAGAGTACATGGGAAGCAGT	3185	0.13164567844644046	No Hit
TTGATACCACTGCTTCCCATGTACT	3097	0.12800837241715105	No Hit
GTACATGGAAGCAGTGGTATCAACG	3090	0.1277190412557303	No Hit
GCTTTCCATGTACTCTGCGTTGATA	3001	0.1240404022033808	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	2991	0.12362707197277971	No Hit
CGCAGAGTACATGGGAAGCAGTGGT	2910	0.12027909710491107	No Hit
CGTTGATACCACTGCTTCCCATGTA	2900	0.11986576687431001	No Hit
ATGTACTCTGCGTTGATACCACTGC	2798	0.11564979852217909	No Hit
ACGCAGAGTACATGGAAAGCAGTGG	2673	0.11048317063966573	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	8.266604612021379E-5	0.0	0.0	0.0	0.0
4	8.266604612021379E-5	0.0	0.0	0.0	0.0
5	8.266604612021379E-5	0.0	0.0	0.0	0.0
6	8.266604612021379E-5	0.0	0.0	0.0	0.0
7	8.266604612021379E-5	0.0	0.0	0.0	0.0
8	8.266604612021379E-5	0.0	0.0	0.0	0.0
9	8.266604612021379E-5	0.0	0.0	0.0	0.0
10	8.266604612021379E-5	0.0	0.0	1.6533209224042759E-4	0.0
11	8.266604612021379E-5	0.0	0.0	2.479981383606414E-4	0.0
12	8.266604612021379E-5	0.0	0.0	4.5466325366117584E-4	0.0
13	8.266604612021379E-5	0.0	0.0	5.786623228414966E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACAAA	405	0.0	17.851473	1
GGTGTCC	35	0.0021716	16.28467	8
GGACGCA	175	0.0	16.284668	8
GGGACGC	180	0.0	15.832317	7
GCAACCC	50	8.723448E-5	15.19871	3
TAGTACA	45	6.7686074E-4	14.776525	4
TACAAAA	465	0.0	14.708734	2
ATACGAC	65	3.3769738E-6	14.614144	19
GTACAAG	215	0.0	14.601282	1
CGCAAAA	360	0.0	14.512958	2
GAACAAA	1070	0.0	14.313888	1
GCTTTCA	40	0.0052326987	14.269434	1
GTCAACA	40	0.0052824756	14.249085	2
TGGGACG	195	0.0	14.1270075	6
GATACGA	75	9.688374E-7	13.932152	18
TTACTCT	150	0.0	13.932152	4
TACAAGA	130	1.8189894E-12	13.883724	2
CATTTAC	125	7.2759576E-12	13.698656	1
TTAGACA	50	0.0015009278	13.298871	4
TACAAGG	130	1.4551915E-11	13.153002	2
>>END_MODULE