FastQCFastQC Report
Wed 25 May 2016
SRR1294995_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294995_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3277883
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC2524107.700396872005498No Hit
CCCATGTACTCTGCGTTGATACCAC2443247.453713265543644No Hit
GAGTACATGGGAAGCAGTGGTATCA1130803.4497875610569384No Hit
CATGTACTCTGCGTTGATACCACTG909362.774229586595983No Hit
GCGTTGATACCACTGCTTCCCATGT734522.2408365399253114No Hit
ACGCAGAGTACATGGGAAGCAGTGG626141.910196306579582No Hit
GTATCAACGCAGAGTACATGGGAAG611381.8651672436142475No Hit
TATCAACGCAGAGTACATGGGAAGC600141.830876818971269No Hit
ACTCTGCGTTGATACCACTGCTTCC552741.6862712915622673No Hit
GGTATCAACGCAGAGTACATGGGAA511171.5594516338746685No Hit
GCTTCCCATGTACTCTGCGTTGATA404041.2326248374331847No Hit
GTGGTATCAACGCAGAGTACATGGG289130.8820632096996751No Hit
ATACCACTGCTTCCCATGTACTCTG274840.8384679990103369No Hit
GTACTCTGCGTTGATACCACTGCTT267920.8173568123084319No Hit
CAGTGGTATCAACGCAGAGTACATG262680.8013708848058335No Hit
GGGAAGCAGTGGTATCAACGCAGAG237680.7251021467209171No Hit
ACATGGGAAGCAGTGGTATCAACGC232250.7085365768088733No Hit
GCAGTGGTATCAACGCAGAGTACAT230200.7022825402859101No Hit
CATGGGAAGCAGTGGTATCAACGCA229430.6999334631528947No Hit
GTTGATACCACTGCTTCCCATGTAC222510.6788222764509899No Hit
TACCACTGCTTCCCATGTACTCTGC219970.6710733726615623No Hit
CTGCTTCCCATGTACTCTGCGTTGA219810.6705852527378189No Hit
GATACCACTGCTTCCCATGTACTCT214730.6550874451589639No Hit
GCAGAGTACATGGGAAGCAGTGGTA201510.6147565364596601No Hit
CTTCCCATGTACTCTGCGTTGATAC189340.5776289147599227No Hit
AAGCAGTGGTATCAACGCAGAGTAC181350.5532534260679834No Hit
CCATGTACTCTGCGTTGATACCACT180740.5513924688587115No Hit
CCACTGCTTCCCATGTACTCTGCGT177680.5420571753171178No Hit
GTACATGGTAAGCAGTGGTATCAAC166750.5087124830263924No Hit
TTCCCATGTACTCTGCGTTGATACC161890.49388584034268457No Hit
ATGGGAAGCAGTGGTATCAACGCAG154260.4706086214791681No Hit
TGATACCACTGCTTCCCATGTACTC133810.4082207937257065No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA133470.4071835388877516No Hit
ACCATGTACTCTGCGTTGATACCAC130170.3971160654605427No Hit
ATCAACGCAGAGTACATGGGAAGCA111640.34058567679200263No Hit
GTATCAACGCAGAGTACTTTTTTTT103460.315630545690618No Hit
CCCCATGTACTCTGCGTTGATACCA82130.25055805835656736No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA81450.24848354868065758No Hit
GGTATCAACGCAGAGTACTTTTTTT80490.24555482913819682No Hit
GTACATGGGTAAGCAGTGGTATCAA79330.2420159596910567No Hit
TATCAACGCAGAGTACTTTTTTTTT77760.23722628293932393No Hit
TCCATGTACTCTGCGTTGATACCAC77620.23679917800604844No Hit
GGTAAGCAGTGGTATCAACGCAGAG73440.2240470449982504No Hit
GGAAGCAGTGGTATCAACGCAGAGT70480.2150168264089963No Hit
AAAAAGTACTCTGCGTTGATACCAC65050.19845125649695247No Hit
GAGTACATGGTAAGCAGTGGTATCA59440.1813365516706972No Hit
GTACATGGAAAGCAGTGGTATCAAC57790.17630281495709274No Hit
CTGCGTTGATACCACTGCTTCCCAT56630.1727639455099526No Hit
GAACAAAAAAAAAAAAAAAAAAAAA54150.16519808669192892No Hit
GTACTTTTTTTTTTTTTTTTTTTTT53540.16333712948265694No Hit
CTCTGCGTTGATACCACTGCTTCCC53330.16269647208274365No Hit
GCTTACCATGTACTCTGCGTTGATA51870.15824237777858455No Hit
TCCCATGTACTCTGCGTTGATACCA51280.15644243555978052No Hit
TGGGAAGCAGTGGTATCAACGCAGA49950.15238493869366296No Hit
TCAACGCAGAGTACATGGGAAGCAG47250.14414791498049198No Hit
AAAGTACTCTGCGTTGATACCACTG47030.14347675008534472No Hit
AGTGGTATCAACGCAGAGTACATGG44370.13536175635310962No Hit
ACTCTGCGTTGATACCACTGCTTAC43240.1319144093916714No Hit
GCGTTGATACCACTGCTTACCATGT40910.12480616300215717No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN39990.12199947344063228No Hit
ACGCAGAGTACTTTTTTTTTTTTTT39360.12007750124089238No Hit
TTGATACCACTGCTTCCCATGTACT38630.11785045408881281No Hit
GAGTACATGGAAAGCAGTGGTATCA38310.1168742142413259No Hit
GTACATGGAAGCAGTGGTATCAACG37760.11519630200345773No Hit
ACTGCTTCCCATGTACTCTGCGTTG36260.11062017771836273No Hit
AACGCAGAGTACATGGGAAGCAGTG35790.10918632544236631No Hit
ACGCAGAGTACATGGTAAGCAGTGG35760.1090948029566644No Hit
CTGCTTACCATGTACTCTGCGTTGA34300.10464070865250528No Hit
TATCAACGCAGAGTACATGGTAAGC33400.1018950340814483No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACCG453.525618E-516.8973625
GTACAAA12700.015.4247311
GTACATA17050.015.0589081
TACATAA12950.015.0452342
TAGTGCG551.9660866E-413.81412317
AGAACAA17500.013.5774062
GTAGGAC500.001495023413.3060623
GTCCTAA957.3012416E-813.0128941
AGTGCGA604.1032722E-412.66333418
GCTGCGT2050.012.51081510
GCGAAAT853.958705E-612.29088312
TACATGA7600.012.2553952
TTGGTCA701.08589134E-412.2211624
TACCGAT701.0922671E-412.2134997
GTACCGA701.0928909E-412.2127526
ACATAAA24700.012.1978653
TACGCTG1950.012.1787037
CGATCAA1052.7271017E-711.76060910
AGGGTGT1052.7276474E-711.7604286
GAGAACA26500.011.6266141
Produced by FastQC (version 0.11.5)