##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294995_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3277883
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.353885114264298	33.0	33.0	33.0	22.0	33.0
2	30.289457860454444	33.0	33.0	33.0	22.0	33.0
3	30.485426111914304	33.0	33.0	33.0	27.0	33.0
4	33.56242001316093	37.0	33.0	37.0	22.0	37.0
5	34.460092382797065	37.0	37.0	37.0	27.0	37.0
6	34.02050805352113	37.0	37.0	37.0	27.0	37.0
7	34.190372871758996	37.0	37.0	37.0	27.0	37.0
8	33.91643691980464	37.0	37.0	37.0	27.0	37.0
9	33.74428220897451	37.0	37.0	37.0	22.0	37.0
10	33.942070232525076	37.0	37.0	37.0	27.0	37.0
11	34.107714033722374	37.0	37.0	37.0	27.0	37.0
12	34.1107626477211	37.0	37.0	37.0	27.0	37.0
13	33.754428086664475	37.0	37.0	37.0	27.0	37.0
14	35.274976562616786	40.0	37.0	40.0	27.0	40.0
15	35.22886783939512	37.0	37.0	40.0	27.0	40.0
16	35.26221405706061	37.0	33.0	40.0	27.0	40.0
17	35.33670420817339	37.0	37.0	40.0	27.0	40.0
18	35.222574143128355	37.0	37.0	40.0	27.0	40.0
19	35.133347041367855	37.0	33.0	40.0	27.0	40.0
20	35.28656452960646	37.0	33.0	40.0	27.0	40.0
21	35.253791852851364	37.0	33.0	40.0	27.0	40.0
22	35.31927832689574	37.0	33.0	40.0	27.0	40.0
23	35.353120291358785	37.0	33.0	40.0	27.0	40.0
24	35.50157189869193	37.0	33.0	40.0	27.0	40.0
25	34.431976675189446	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.49846207649339647
1101	2	0.4647350227969689
1101	3	0.39379189600120057
1101	4	0.5211142725978135
1101	5	0.46798869628959494
1101	6	0.2668510387083529
1101	7	0.2547447909196592
1101	8	0.35255225208125296
1101	9	0.35959475132481344
1101	10	0.5856774519261592
1101	11	0.4165903480742372
1101	12	0.40239441506700047
1101	13	0.3135861995359335
1101	14	1.0267752020622822
1101	15	0.7443032633966098
1101	16	0.777379340820147
1101	17	0.776224424507781
1101	18	0.7055746430244199
1101	19	0.70520964226111
1101	20	0.669155080579273
1101	21	0.6813298826189964
1101	22	0.6967856153901195
1101	23	0.8728639029169045
1101	24	0.7491324013420666
1101	25	0.8631811849391156
1102	1	0.5107399949905975
1102	2	0.5715572920576761
1102	3	0.4776598238920471
1102	4	0.6301149475144854
1102	5	0.4841813614285968
1102	6	0.37489459670347003
1102	7	0.41110856525603623
1102	8	0.38401229115053326
1102	9	0.4436677986943067
1102	10	0.5090872183268118
1102	11	0.33624454238815105
1102	12	0.5241082205047363
1102	13	0.5012200244738167
1102	14	0.7813554487345513
1102	15	0.7880866599866181
1102	16	0.7759012388192019
1102	17	0.8770815341857627
1102	18	0.8992224698962232
1102	19	0.7808222929819593
1102	20	0.774340119274882
1102	21	0.7662236378576495
1102	22	0.8117312138871355
1102	23	0.8126115277736119
1102	24	0.907840470562995
1102	25	1.010849273108299
1103	1	0.27816615691547497
1103	2	0.29608418775310597
1103	3	0.25132309402780706
1103	4	0.24407105087973235
1103	5	0.10046092305084642
1103	6	0.03344104683382909
1103	7	0.05523196139574793
1103	8	0.14338152081069921
1103	9	0.13586580649523938
1103	10	0.3144061841055148
1103	11	0.08504384370819196
1103	12	0.1685765376744044
1103	13	0.07760403529385229
1103	14	0.5900432107384077
1103	15	0.5285786969232689
1103	16	0.4640429483081263
1103	17	0.4575685977796624
1103	18	0.5315428618885107
1103	19	0.490262077330037
1103	20	0.47473788769656267
1103	21	0.42781020799623093
1103	22	0.37784085296518555
1103	23	0.53233684373523
1103	24	0.5892975969914644
1103	25	0.5398691240898117
1104	1	0.4164265235526692
1104	2	0.37266903521465977
1104	3	0.37905132867084745
1104	4	0.4621134818116488
1104	5	0.22495131682296687
1104	6	0.26493266081546807
1104	7	0.2550212417102742
1104	8	0.3706796659644169
1104	9	0.3609846558824046
1104	10	0.47987187347223426
1104	11	0.3312958165786881
1104	12	0.4206294742976695
1104	13	0.3937245815225623
1104	14	0.5819363513083502
1104	15	0.7271914560755235
1104	16	0.4978589576635599
1104	17	0.6241540747386978
1104	18	0.8228135353869064
1104	19	0.6525577346992009
1104	20	0.6754398955834091
1104	21	0.5549343351813363
1104	22	0.6601573844358128
1104	23	0.6154106370371437
1104	24	0.7423169654645321
1104	25	0.874081506734278
1105	1	0.3715583521687158
1105	2	0.4399021253217441
1105	3	0.34010648017469336
1105	4	0.48981631656939584
1105	5	0.42687949791476854
1105	6	0.2741521851957742
1105	7	0.30728494814136553
1105	8	0.40536967538344726
1105	9	0.4276727120227406
1105	10	0.4696755349697028
1105	11	0.38397679689004605
1105	12	0.46588075116666516
1105	13	0.42837006716316495
1105	14	0.8362774524753291
1105	15	0.6795506636081896
1105	16	0.7170245698216036
1105	17	0.5485097918518207
1105	18	0.6181723312821674
1105	19	0.6416735278514238
1105	20	0.6593355269530861
1105	21	0.7199224612270569
1105	22	0.6553144295023117
1105	23	0.7858416311447627
1105	24	0.7617974511818204
1105	25	0.7104578444302305
1106	1	0.03233059642285596
1106	2	-0.21977513715613028
1106	3	-0.21197647937945518
1106	4	0.002662962283103809
1106	5	-0.47427585412565065
1106	6	-0.26224637389184124
1106	7	-0.7598570590201703
1106	8	-0.22677541797720835
1106	9	-0.015173958725483772
1106	10	-0.3180007810181493
1106	11	-0.32270475696693524
1106	12	-0.9075963043777833
1106	13	-0.2434696570640611
1106	14	-0.2652298013674894
1106	15	-0.10712093393030386
1106	16	-0.10012487815347981
1106	17	-0.08645307501157617
1106	18	-0.4134715247708627
1106	19	-0.5045941368949087
1106	20	-0.0757365552906677
1106	21	-0.06170621185752623
1106	22	0.24013914757892962
1106	23	-0.5222436542083528
1106	24	0.1875160181951756
1106	25	0.2817335132666443
1107	1	-1.1008490795193886
1107	2	-1.122888259608903
1107	3	-0.8706349705105971
1107	4	-0.9577677316785795
1107	5	-1.0866160732765877
1107	6	-0.900371363213921
1107	7	-1.0756622227034711
1107	8	-1.090730573867809
1107	9	-1.1000551976357045
1107	10	-0.909492826711606
1107	11	-0.9186983505659683
1107	12	-1.0095173181330281
1107	13	-0.9667850654450945
1107	14	-1.093728261481246
1107	15	-1.0229906358098617
1107	16	-1.122645779847268
1107	17	-1.059989538046466
1107	18	-1.1712956914837278
1107	19	-1.1328880342871983
1107	20	-1.1945028750688849
1107	21	-1.1398081508276192
1107	22	-1.199916687897833
1107	23	-0.9444107987226573
1107	24	-0.9545699985638336
1107	25	-0.8057061543285187
1108	1	0.02200621277585313
1108	2	-0.07067544100491929
1108	3	-0.013845982973879956
1108	4	-0.1356287906941276
1108	5	-0.42162202299594753
1108	6	-0.1304896031049907
1108	7	-0.08292728528839888
1108	8	0.0948808702332542
1108	9	-0.1576490885271653
1108	10	-0.4325876077734847
1108	11	-0.07753122007669333
1108	12	0.07863167789964365
1108	13	0.02947852879739088
1108	14	-0.21654661646672224
1108	15	0.08459226013822274
1108	16	-0.6636686935792682
1108	17	-0.8139398427023963
1108	18	-0.6098627578633398
1108	19	-0.013053313936005395
1108	20	-1.0658170378009473
1108	21	-0.21790123614822932
1108	22	-0.3264618861900601
1108	23	-0.16025466212676065
1108	24	-0.5788145087046104
1108	25	-1.1234842427713687
1109	1	0.09147421455261195
1109	2	0.059137616705928764
1109	3	0.03424256934149028
1109	4	0.08595400782671447
1109	5	-0.008111921618322526
1109	6	-0.04742397922564834
1109	7	0.03874937224220787
1109	8	0.1921520385035862
1109	9	0.17186764163393065
1109	10	0.223068951208802
1109	11	0.12832513677907542
1109	12	0.0936014549783053
1109	13	0.15293405397704873
1109	14	0.46557162667425445
1109	15	0.30695602849377934
1109	16	0.27933386181923936
1109	17	0.3445241179993701
1109	18	0.3805388157777827
1109	19	0.36117815993425495
1109	20	0.3074653306770969
1109	21	0.4238602889056793
1109	22	0.29253904730612135
1109	23	0.33293572044657793
1109	24	0.4222010722694165
1109	25	0.2510999846652169
1110	1	-0.11237942997776429
1110	2	-0.06597303950505307
1110	3	-0.06975179473080573
1110	4	-1.100901849399115
1110	5	-0.2563952675477168
1110	6	-0.09821839577836045
1110	7	-0.017593540386478423
1110	8	-0.07323203323336713
1110	9	-0.06735086325738138
1110	10	-0.12157351596520982
1110	11	-0.13112412472202095
1110	12	0.035076003120799726
1110	13	0.04270460366079476
1110	14	0.10337612598365808
1110	15	-0.07089509291776608
1110	16	-0.06441138607354446
1110	17	0.05523512470863068
1110	18	0.11368080473974373
1110	19	-0.05763319584408322
1110	20	0.13532582052859254
1110	21	0.10329935731544282
1110	22	-0.48176212138353947
1110	23	-0.02672483474817966
1110	24	0.1616463920226323
1110	25	0.30544933471986724
1111	1	-0.08469651033924919
1111	2	-0.21908149337730976
1111	3	-0.08222590008891828
1111	4	-0.007300735730410679
1111	5	-0.0353899308691652
1111	6	0.017248163280982
1111	7	0.13332461032879195
1111	8	0.17885934421766336
1111	9	0.12034166111704536
1111	10	0.13022597476301456
1111	11	0.022519163713759838
1111	12	0.10079232862884879
1111	13	-0.017175175058611103
1111	14	-0.04028142983041505
1111	15	0.07265997418823389
1111	16	-0.065647747809642
1111	17	0.023244675231019585
1111	18	-0.06027264372932706
1111	19	-0.03228093370102414
1111	20	0.03647514720503153
1111	21	-0.09426711922197484
1111	22	-0.03356942975759836
1111	23	0.31169137727475515
1111	24	0.2523939951002845
1111	25	0.19529304607701192
1112	1	-0.13294875743265067
1112	2	-0.4116354822145105
1112	3	-0.20704283984367677
1112	4	-0.18450121776412942
1112	5	-0.0676612836605841
1112	6	0.013388059646999295
1112	7	0.0015533185801146487
1112	8	0.08784662339996885
1112	9	-0.012699972207229848
1112	10	0.05680702252834635
1112	11	-0.059791669727658814
1112	12	-2.0539072096426025E-5
1112	13	-0.021055025815229556
1112	14	-0.28926732501472685
1112	15	-0.23222992319742275
1112	16	-0.18838899646948448
1112	17	-0.291506921600849
1112	18	-0.19671486072552824
1112	19	-0.10575549057015365
1112	20	-0.8601718797415998
1112	21	-1.2454454140548776
1112	22	-1.7132008131764351
1112	23	-0.5787382970017276
1112	24	-0.3156663833755786
1112	25	-1.6315601041226628
1113	1	-0.26107165087314854
1113	2	-0.20898671470185803
1113	3	-0.2764820508981316
1113	4	-0.5265187136110256
1113	5	-0.33545346526401687
1113	6	-0.28486206758679344
1113	7	-0.17602357384251377
1113	8	-0.18282536307964392
1113	9	-0.24130683418977128
1113	10	-0.19072089688704352
1113	11	-0.3008690758867374
1113	12	-0.21113030582614556
1113	13	-0.35512756348123276
1113	14	-0.6267814167574954
1113	15	-0.464501502528897
1113	16	-0.4597733001405402
1113	17	-0.49983242885557644
1113	18	-0.8169359114365946
1113	19	-1.046137576052054
1113	20	-1.1219341397978724
1113	21	-1.0692320837943612
1113	22	-0.46652051088814517
1113	23	-2.0241489500711793
1113	24	-1.1814670920438033
1113	25	-0.45261110326803333
1114	1	-0.39364507710376273
1114	2	-0.525417522773381
1114	3	-0.4030069818073123
1114	4	-0.5851932728698088
1114	5	-0.6965022363359523
1114	6	-0.4325728482058224
1114	7	-0.38908422056935876
1114	8	-0.16448985438124453
1114	9	-1.2751266526846692
1114	10	-0.32672171801075933
1114	11	-0.42150599826603496
1114	12	-0.4713870601814705
1114	13	-0.48270739325126044
1114	14	-0.8687082387023324
1114	15	-1.4088362391258897
1114	16	-1.3455812797472575
1114	17	-1.7686500107075176
1114	18	-1.9573554198653724
1114	19	-1.7027819375670887
1114	20	-1.011327304862121
1114	21	-0.7718714450336464
1114	22	-0.49769416629806074
1114	23	-0.34636475854100723
1114	24	-0.9168061732431312
1114	25	-0.6538558410293049
1115	1	-0.7804403917997966
1115	2	-0.6256142583154514
1115	3	-0.5506751071713545
1115	4	-0.719418986734226
1115	5	-0.5464758318396434
1115	6	-0.4924435739473694
1115	7	-0.4707556178374972
1115	8	-0.24717995573238483
1115	9	-0.7974254090279942
1115	10	-0.3997305177133086
1115	11	-0.7579779068702237
1115	12	-0.744918475408582
1115	13	-0.6142041117945141
1115	14	-0.7443850682424511
1115	15	-1.7813442229344005
1115	16	-1.1291992493051808
1115	17	-0.7618550168824427
1115	18	-2.3081442970570336
1115	19	-1.2979971513577837
1115	20	-0.9614978808475527
1115	21	-0.9645636455177424
1115	22	-0.6820912399548504
1115	23	-0.45709769641108267
1115	24	-0.48239091281593005
1115	25	-0.7014196955762912
1116	1	-1.527043245049022
1116	2	-0.8830171406338287
1116	3	-1.640572147707914
1116	4	-1.0363205876495343
1116	5	-1.0735873517961139
1116	6	-0.7360812803663066
1116	7	-1.5040866012072058
1116	8	-2.9770980465598242
1116	9	-1.7175017279787212
1116	10	-1.0132409037243377
1116	11	-0.8569283116643973
1116	12	-0.9526261179871938
1116	13	-2.000765529077242
1116	14	-1.9942474245582247
1116	15	-1.7700861683564426
1116	16	-1.2615753917291244
1116	17	-2.045415851539829
1116	18	-3.0014401303541334
1116	19	-1.7187068424421241
1116	20	-1.1642752893946309
1116	21	-0.9839611562540753
1116	22	-0.7494767056265417
1116	23	-0.7105669880802878
1116	24	-0.7560985777554237
1116	25	-0.8010950900285607
1201	1	0.5167772043541063
1201	2	0.5754398407000032
1201	3	0.538200746141861
1201	4	0.5965285210201472
1201	5	0.48889651999641615
1201	6	0.25270348567593715
1201	7	0.2610924605346838
1201	8	0.29117049472514367
1201	9	0.26634995319013655
1201	10	0.5761871206794922
1201	11	0.2723213970642746
1201	12	0.40088644436471554
1201	13	0.37728541640493063
1201	14	0.9009156587619813
1201	15	0.7895155197769768
1201	16	0.5591377106911466
1201	17	0.6947118918773185
1201	18	0.7119354915430591
1201	19	0.6836246256607552
1201	20	0.5694774568369709
1201	21	0.6317351339052095
1201	22	0.6729201415831909
1201	23	0.7606931854269803
1201	24	0.7590059345554394
1201	25	0.9870730165532748
1202	1	0.506663596335784
1202	2	0.6061908987532654
1202	3	0.5464250101429009
1202	4	0.7173960319305124
1202	5	0.44402021894650545
1202	6	0.3101517065178854
1202	7	0.20648186330407015
1202	8	0.2489174236392202
1202	9	0.20924702980906318
1202	10	0.5056876865550279
1202	11	0.3427991856762631
1202	12	0.33780111363159193
1202	13	0.46878718206030356
1202	14	0.8507073464728947
1202	15	0.7367004693429351
1202	16	0.6631598592041001
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2210	20	0.4291386076726269
2210	21	0.4668161943453697
2210	22	0.5727649246058917
2210	23	0.6173696496225816
2210	24	0.5766636312275821
2210	25	0.6600556034302585
2211	1	0.05029818484619142
2211	2	0.019148017358276093
2211	3	0.016335925331482315
2211	4	-0.07722774057935311
2211	5	0.030817991344562756
2211	6	0.03908873787660383
2211	7	0.11998288181963801
2211	8	0.16469378971432036
2211	9	-0.06414204544334723
2211	10	0.231889163186473
2211	11	0.02226351424845774
2211	12	0.007422549907737164
2211	13	-0.09868295963772056
2211	14	-0.15733781912059186
2211	15	-0.07003075723864072
2211	16	-0.11318324464973983
2211	17	-0.05446317095785247
2211	18	-0.10197531289311002
2211	19	-0.15284180112353596
2211	20	-0.004413393013251721
2211	21	-0.04382028627102841
2211	22	-0.07850054615519042
2211	23	0.16939797800995393
2211	24	0.21436721262758596
2211	25	0.07365975559405769
2212	1	0.2117765904665312
2212	2	0.1582086423968967
2212	3	0.182936541603695
2212	4	0.1807634604217796
2212	5	0.21330944091860005
2212	6	0.18184705768985054
2212	7	0.2424974987360642
2212	8	0.3909653264215862
2212	9	0.2558036056890245
2212	10	0.439641378590494
2212	11	0.2683627747197477
2212	12	0.3447579529909035
2212	13	0.24827653420206275
2212	14	0.25063174256202814
2212	15	0.25739298735037863
2212	16	0.17772411149901046
2212	17	0.21378956603419397
2212	18	0.21007042410990096
2212	19	0.22279022531383674
2212	20	0.2716131013423393
2212	21	0.19418721160167962
2212	22	-0.48371710280106583
2212	23	0.2186233027047777
2212	24	0.16926591263899127
2212	25	0.29438715849357067
2213	1	-0.28298175487121213
2213	2	-1.0347808394623286
2213	3	-0.35292769842097016
2213	4	-0.48406852663776334
2213	5	-0.21738142939491922
2213	6	-0.33753587830659626
2213	7	-0.2579267942477159
2213	8	-0.015217504621197975
2213	9	-0.35087670265733806
2213	10	-1.6651810929764252
2213	11	-0.7603667596507577
2213	12	-0.2920527072698391
2213	13	-0.3493635776949233
2213	14	-0.3366580702151083
2213	15	-0.4494176901347089
2213	16	-0.4325978057311346
2213	17	-0.3632900389465519
2213	18	-0.19914647496099747
2213	19	-0.353070470323793
2213	20	-0.29988543372729026
2213	21	-0.30037420208053334
2213	22	-0.21732254247825722
2213	23	-1.1402383251525734
2213	24	-1.562091080434719
2213	25	-1.4942964672612646
2214	1	-0.13238691729243968
2214	2	-0.2600603299922781
2214	3	-0.038056690759798784
2214	4	-0.08950566186555875
2214	5	-0.1604860484758177
2214	6	-0.088706763507588
2214	7	-0.05029151306974455
2214	8	0.03647675929047267
2214	9	-0.37372531462446545
2214	10	-0.10015538278611302
2214	11	-0.20593298082621203
2214	12	-0.15007521281476244
2214	13	-0.17517076162039302
2214	14	-0.20857826243183553
2214	15	-0.24013030621119924
2214	16	-0.2869747745385638
2214	17	-0.2586518643260476
2214	18	-0.14926251890581455
2214	19	-0.8324061675588794
2214	20	-0.4434767195021294
2214	21	-0.33571500453008696
2214	22	-0.5322531870190232
2214	23	-1.7387701048522075
2214	24	-1.3222483506972793
2214	25	-1.8642389716060137
2215	1	-0.32012461787583746
2215	2	-0.3039881821807313
2215	3	-1.2311404877037013
2215	4	-0.8085219121159923
2215	5	-0.31744857766091883
2215	6	-0.29805326510945207
2215	7	-0.24157280027150563
2215	8	-0.17183262859748538
2215	9	-0.24710469346753428
2215	10	-1.3436808916161027
2215	11	-0.5583420173732634
2215	12	-0.46569584223459515
2215	13	-0.4631881553843442
2215	14	-0.7973248631166499
2215	15	-1.0840302920684692
2215	16	-0.757062776819609
2215	17	-0.5034659664380072
2215	18	-0.46889544495800806
2215	19	-1.1284143245980545
2215	20	-1.5113891361142038
2215	21	-0.8272319999384266
2215	22	-0.48185165198773916
2215	23	-0.7426221730740608
2215	24	-1.9914780532125178
2215	25	-0.8839983200296402
2216	1	-0.7189339609588536
2216	2	-0.6652133380532987
2216	3	-1.453173596971272
2216	4	-0.9602405117298574
2216	5	-0.83662485573646
2216	6	-0.6097697840808891
2216	7	-0.6048382222642843
2216	8	-0.7528485719264495
2216	9	-0.6971886361270236
2216	10	-0.538346673622712
2216	11	-0.5981878872835296
2216	12	-0.5832913835757765
2216	13	-1.3180950144160022
2216	14	-1.0566756643642705
2216	15	-1.4051590765779451
2216	16	-1.1947000192508455
2216	17	-2.1701014486248624
2216	18	-1.3333160472888963
2216	19	-0.9782014331953377
2216	20	-0.8536870640230774
2216	21	-0.746829103038614
2216	22	-0.6856326680878126
2216	23	-0.5209469644916922
2216	24	-0.6573204646352906
2216	25	-0.7153624255458411
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	4433.0
3	450.0
4	535.0
5	493.0
6	343.0
7	224.0
8	269.0
9	560.0
10	1088.0
11	2229.0
12	4534.0
13	8459.0
14	15509.0
15	26972.0
16	36812.0
17	39024.0
18	36975.0
19	34053.0
20	33349.0
21	33816.0
22	32888.0
23	30834.0
24	30595.0
25	29773.0
26	28967.0
27	29613.0
28	31471.0
29	35967.0
30	42787.0
31	53913.0
32	72991.0
33	104740.0
34	154518.0
35	252373.0
36	471089.0
37	1595237.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.122241234152064	18.019953243098247	10.386348869898722	23.471456652850968
2	10.505966207790303	25.755984546543758	33.64091498153899	30.09713426412695
3	22.952066023740006	33.72136780929981	24.436053334768083	18.890512832192105
4	13.178273241126515	23.680665249135142	26.709461686593443	36.4315998231449
5	9.191988175123413	44.91859693056004	33.51507179367511	12.37434310064143
6	31.734928599704403	29.282237122651388	20.113959243063633	18.86887503458058
7	21.147980752359953	37.68261690630439	21.8321999984105	19.33720234292516
8	29.143265791444122	35.49677730621874	21.00696148329828	14.352995419038859
9	28.44498908177448	20.401335747511677	17.192887835488545	33.9607873352253
10	17.38199413093393	36.238471095140085	28.624707869177985	17.754826904748
11	24.353385226164235	32.46755271489706	14.561407960127523	28.61765409881118
12	24.74382342205072	37.35534178379241	24.55348326162203	13.347351532534837
13	30.318651596621287	23.85138601734119	18.738308698664525	27.091653687372997
14	20.880101206319157	33.59717170160767	22.218339548668244	23.30438754340493
15	38.40455307457255	22.163040567556962	18.859658776186965	20.572747581683522
16	15.358442530337935	29.82456944831668	35.25681472459855	19.560173296746832
17	27.904707461199134	32.697122453908214	25.1491658998713	14.249004185021352
18	28.832069927350588	24.671176123155487	28.118752010056703	18.378001939437222
19	22.484349119643714	35.69085514123548	29.33900366303054	12.485792076090272
20	24.406226709603025	28.810759850061046	25.76285507633595	21.02015836399998
21	17.21148608289431	32.578322121677694	31.09624986778948	19.113941927638507
22	21.0244848610833	31.403198515531738	17.07199640499449	30.50032021839047
23	14.433831500389172	33.0656625471025	26.575224285231265	25.925281667277073
24	20.043504171086237	42.368072561581734	19.798923158632757	17.789500108699276
25	21.117080945594875	25.702058548269285	17.517243234864186	35.663617271271654
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	21926.0
1	21926.0
2	24293.0
3	26660.0
4	26660.0
5	26660.0
6	33942.0
7	41224.0
8	41224.0
9	41224.0
10	40763.5
11	40303.0
12	40303.0
13	40303.0
14	41180.0
15	42057.0
16	42057.0
17	42057.0
18	46379.5
19	50702.0
20	50702.0
21	50702.0
22	57782.0
23	64862.0
24	64862.0
25	64862.0
26	74457.0
27	84052.0
28	84052.0
29	84052.0
30	90963.5
31	97875.0
32	97875.0
33	97875.0
34	102662.5
35	107450.0
36	107450.0
37	107450.0
38	114539.5
39	121629.0
40	121629.0
41	121629.0
42	179473.0
43	237317.0
44	237317.0
45	237317.0
46	708974.0
47	1180631.0
48	1180631.0
49	1180631.0
50	976982.5
51	773334.0
52	773334.0
53	773334.0
54	502298.5
55	231263.0
56	231263.0
57	231263.0
58	151087.5
59	70912.0
60	70912.0
61	70912.0
62	57148.5
63	43385.0
64	43385.0
65	43385.0
66	33545.0
67	23705.0
68	23705.0
69	23705.0
70	17432.0
71	11159.0
72	11159.0
73	11159.0
74	7942.0
75	4725.0
76	4725.0
77	4725.0
78	3313.0
79	1901.0
80	1901.0
81	1901.0
82	1234.5
83	568.0
84	568.0
85	568.0
86	359.5
87	151.0
88	151.0
89	151.0
90	107.5
91	64.0
92	64.0
93	64.0
94	37.5
95	11.0
96	11.0
97	11.0
98	14.0
99	17.0
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.31556953070015004
2	0.18679739331757722
3	0.20427818808664006
4	0.2260300321884582
5	0.2491242060805709
6	0.19945800383967335
7	0.1957665969163634
8	0.21879975581800812
9	0.21980650316072906
10	0.22929433417849263
11	0.20299687328681348
12	0.21041019462866734
13	0.22236913276038223
14	0.2117525244189619
15	0.2013189610489453
16	0.20534595041982892
17	0.20418666560093815
18	0.20839669994322552
19	0.20809162499088588
20	0.20394260563906644
21	0.20089185611566976
22	0.20372905317242868
23	0.20821365497182176
24	0.2255114047694808
25	0.21904381577987986
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3277883.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.09480257895707
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.73188451910717	15.017224657648768
2	12.010926079315597	4.827143767085875
3	4.988243364792958	3.0071329689392106
4	2.6309391799080104	2.114728136699787
5	1.486045222225726	1.4930892682014174
6	0.963888973826428	1.1621495182245356
7	0.6237844568621879	0.8774377858727341
8	0.4608536618069382	0.7408610681439897
9	0.32700385404608207	0.59139701006527
>10	1.535884917317733	5.430893172298697
>50	0.11541162878183223	1.613599382699054
>100	0.09453570774831795	3.942236586979675
>500	0.010047247070835805	1.4116608267779127
>1k	0.012482943330432362	5.45872572939486
>5k	0.0025879272758213435	3.443819805339542
>10k+	0.005480316584092257	48.86790031562862
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	252410	7.700396872005498	No Hit
CCCATGTACTCTGCGTTGATACCAC	244324	7.453713265543644	No Hit
GAGTACATGGGAAGCAGTGGTATCA	113080	3.4497875610569384	No Hit
CATGTACTCTGCGTTGATACCACTG	90936	2.774229586595983	No Hit
GCGTTGATACCACTGCTTCCCATGT	73452	2.2408365399253114	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	62614	1.910196306579582	No Hit
GTATCAACGCAGAGTACATGGGAAG	61138	1.8651672436142475	No Hit
TATCAACGCAGAGTACATGGGAAGC	60014	1.830876818971269	No Hit
ACTCTGCGTTGATACCACTGCTTCC	55274	1.6862712915622673	No Hit
GGTATCAACGCAGAGTACATGGGAA	51117	1.5594516338746685	No Hit
GCTTCCCATGTACTCTGCGTTGATA	40404	1.2326248374331847	No Hit
GTGGTATCAACGCAGAGTACATGGG	28913	0.8820632096996751	No Hit
ATACCACTGCTTCCCATGTACTCTG	27484	0.8384679990103369	No Hit
GTACTCTGCGTTGATACCACTGCTT	26792	0.8173568123084319	No Hit
CAGTGGTATCAACGCAGAGTACATG	26268	0.8013708848058335	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	23768	0.7251021467209171	No Hit
ACATGGGAAGCAGTGGTATCAACGC	23225	0.7085365768088733	No Hit
GCAGTGGTATCAACGCAGAGTACAT	23020	0.7022825402859101	No Hit
CATGGGAAGCAGTGGTATCAACGCA	22943	0.6999334631528947	No Hit
GTTGATACCACTGCTTCCCATGTAC	22251	0.6788222764509899	No Hit
TACCACTGCTTCCCATGTACTCTGC	21997	0.6710733726615623	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	21981	0.6705852527378189	No Hit
GATACCACTGCTTCCCATGTACTCT	21473	0.6550874451589639	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	20151	0.6147565364596601	No Hit
CTTCCCATGTACTCTGCGTTGATAC	18934	0.5776289147599227	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	18135	0.5532534260679834	No Hit
CCATGTACTCTGCGTTGATACCACT	18074	0.5513924688587115	No Hit
CCACTGCTTCCCATGTACTCTGCGT	17768	0.5420571753171178	No Hit
GTACATGGTAAGCAGTGGTATCAAC	16675	0.5087124830263924	No Hit
TTCCCATGTACTCTGCGTTGATACC	16189	0.49388584034268457	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	15426	0.4706086214791681	No Hit
TGATACCACTGCTTCCCATGTACTC	13381	0.4082207937257065	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	13347	0.4071835388877516	No Hit
ACCATGTACTCTGCGTTGATACCAC	13017	0.3971160654605427	No Hit
ATCAACGCAGAGTACATGGGAAGCA	11164	0.34058567679200263	No Hit
GTATCAACGCAGAGTACTTTTTTTT	10346	0.315630545690618	No Hit
CCCCATGTACTCTGCGTTGATACCA	8213	0.25055805835656736	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	8145	0.24848354868065758	No Hit
GGTATCAACGCAGAGTACTTTTTTT	8049	0.24555482913819682	No Hit
GTACATGGGTAAGCAGTGGTATCAA	7933	0.2420159596910567	No Hit
TATCAACGCAGAGTACTTTTTTTTT	7776	0.23722628293932393	No Hit
TCCATGTACTCTGCGTTGATACCAC	7762	0.23679917800604844	No Hit
GGTAAGCAGTGGTATCAACGCAGAG	7344	0.2240470449982504	No Hit
GGAAGCAGTGGTATCAACGCAGAGT	7048	0.2150168264089963	No Hit
AAAAAGTACTCTGCGTTGATACCAC	6505	0.19845125649695247	No Hit
GAGTACATGGTAAGCAGTGGTATCA	5944	0.1813365516706972	No Hit
GTACATGGAAAGCAGTGGTATCAAC	5779	0.17630281495709274	No Hit
CTGCGTTGATACCACTGCTTCCCAT	5663	0.1727639455099526	No Hit
GAACAAAAAAAAAAAAAAAAAAAAA	5415	0.16519808669192892	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	5354	0.16333712948265694	No Hit
CTCTGCGTTGATACCACTGCTTCCC	5333	0.16269647208274365	No Hit
GCTTACCATGTACTCTGCGTTGATA	5187	0.15824237777858455	No Hit
TCCCATGTACTCTGCGTTGATACCA	5128	0.15644243555978052	No Hit
TGGGAAGCAGTGGTATCAACGCAGA	4995	0.15238493869366296	No Hit
TCAACGCAGAGTACATGGGAAGCAG	4725	0.14414791498049198	No Hit
AAAGTACTCTGCGTTGATACCACTG	4703	0.14347675008534472	No Hit
AGTGGTATCAACGCAGAGTACATGG	4437	0.13536175635310962	No Hit
ACTCTGCGTTGATACCACTGCTTAC	4324	0.1319144093916714	No Hit
GCGTTGATACCACTGCTTACCATGT	4091	0.12480616300215717	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	3999	0.12199947344063228	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	3936	0.12007750124089238	No Hit
TTGATACCACTGCTTCCCATGTACT	3863	0.11785045408881281	No Hit
GAGTACATGGAAAGCAGTGGTATCA	3831	0.1168742142413259	No Hit
GTACATGGAAGCAGTGGTATCAACG	3776	0.11519630200345773	No Hit
ACTGCTTCCCATGTACTCTGCGTTG	3626	0.11062017771836273	No Hit
AACGCAGAGTACATGGGAAGCAGTG	3579	0.10918632544236631	No Hit
ACGCAGAGTACATGGTAAGCAGTGG	3576	0.1090948029566644	No Hit
CTGCTTACCATGTACTCTGCGTTGA	3430	0.10464070865250528	No Hit
TATCAACGCAGAGTACATGGTAAGC	3340	0.1018950340814483	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.0507495233966558E-5	0.0	0.0	3.0507495233966558E-5	0.0
2	3.0507495233966558E-5	0.0	0.0	3.0507495233966558E-5	0.0
3	3.0507495233966558E-5	0.0	0.0	3.0507495233966558E-5	0.0
4	3.0507495233966558E-5	0.0	0.0	3.0507495233966558E-5	0.0
5	3.0507495233966558E-5	0.0	0.0	3.0507495233966558E-5	0.0
6	3.0507495233966558E-5	0.0	0.0	3.0507495233966558E-5	0.0
7	3.0507495233966558E-5	0.0	0.0	3.0507495233966558E-5	0.0
8	3.0507495233966558E-5	0.0	0.0	3.0507495233966558E-5	0.0
9	3.0507495233966558E-5	0.0	0.0	9.152248570189967E-5	0.0
10	3.0507495233966558E-5	0.0	0.0	3.9659743804156526E-4	0.0
11	3.0507495233966558E-5	0.0	0.0	5.49134914211398E-4	0.0
12	3.0507495233966558E-5	0.0	0.0	9.457323522529633E-4	0.0
13	3.0507495233966558E-5	0.0	0.0	9.762398474869299E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTACCG	45	3.525618E-5	16.897362	5
GTACAAA	1270	0.0	15.424731	1
GTACATA	1705	0.0	15.058908	1
TACATAA	1295	0.0	15.045234	2
TAGTGCG	55	1.9660866E-4	13.814123	17
AGAACAA	1750	0.0	13.577406	2
GTAGGAC	50	0.0014950234	13.306062	3
GTCCTAA	95	7.3012416E-8	13.012894	1
AGTGCGA	60	4.1032722E-4	12.663334	18
GCTGCGT	205	0.0	12.510815	10
GCGAAAT	85	3.958705E-6	12.290883	12
TACATGA	760	0.0	12.255395	2
TTGGTCA	70	1.08589134E-4	12.221162	4
TACCGAT	70	1.0922671E-4	12.213499	7
GTACCGA	70	1.0928909E-4	12.212752	6
ACATAAA	2470	0.0	12.197865	3
TACGCTG	195	0.0	12.178703	7
CGATCAA	105	2.7271017E-7	11.760609	10
AGGGTGT	105	2.7276474E-7	11.760428	6
GAGAACA	2650	0.0	11.626614	1
>>END_MODULE