FastQCFastQC Report
Wed 25 May 2016
SRR1294995_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294995_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3277883
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC2728168.32293281974982No Hit
GTACATGGGAAGCAGTGGTATCAAC2466837.525680446800573No Hit
GAGTACATGGGAAGCAGTGGTATCA1212793.69991851448023No Hit
CATGTACTCTGCGTTGATACCACTG1013583.0921787019243823No Hit
GCGTTGATACCACTGCTTCCCATGT776882.3700662897363935No Hit
ACGCAGAGTACATGGGAAGCAGTGG765342.3348606402363967No Hit
TATCAACGCAGAGTACATGGGAAGC643241.962364123429665No Hit
GTATCAACGCAGAGTACATGGGAAG631491.926517816529754No Hit
ACTCTGCGTTGATACCACTGCTTCC617161.88280057585948No Hit
GGTATCAACGCAGAGTACATGGGAA548271.6726344411926843No Hit
GCTTCCCATGTACTCTGCGTTGATA440061.3425128352659323No Hit
CAGTGGTATCAACGCAGAGTACATG324040.9885648755614522No Hit
GTATCAACGCAGAGTACTTTTTTTT313660.9568980955085951No Hit
GTGGTATCAACGCAGAGTACATGGG307760.9388986733205547No Hit
ACATGGGAAGCAGTGGTATCAACGC293120.8942357002980277No Hit
ATACCACTGCTTCCCATGTACTCTG290380.885876646603921No Hit
CATGGGAAGCAGTGGTATCAACGCA285220.8701347790631941No Hit
GGGAAGCAGTGGTATCAACGCAGAG273900.835600294458344No Hit
GCAGTGGTATCAACGCAGAGTACAT267410.8158009300514998No Hit
GTACTCTGCGTTGATACCACTGCTT257370.7851714048365974No Hit
TATCAACGCAGAGTACTTTTTTTTT247790.7559452244024574No Hit
GGTATCAACGCAGAGTACTTTTTTT244840.7469455133084372No Hit
CTGCTTCCCATGTACTCTGCGTTGA235520.7185125277503803No Hit
GATACCACTGCTTCCCATGTACTCT234240.7146075683604326No Hit
TACCACTGCTTCCCATGTACTCTGC232930.710611086484783No Hit
GCAGAGTACATGGGAAGCAGTGGTA229820.7011232554670195No Hit
CCATGTACTCTGCGTTGATACCACT214530.6544772952542846No Hit
CTTCCCATGTACTCTGCGTTGATAC214400.654080697816243No Hit
GTTGATACCACTGCTTCCCATGTAC207410.6327559586477003No Hit
AAGCAGTGGTATCAACGCAGAGTAC205260.6261968471723975No Hit
GTACTTTTTTTTTTTTTTTTTTTTT205110.625739234743888No Hit
CCACTGCTTCCCATGTACTCTGCGT201910.6159768362690188No Hit
ATGGGAAGCAGTGGTATCAACGCAG180370.5502636915350548No Hit
GTACATGGTAAGCAGTGGTATCAAC171940.524545873052821No Hit
ACGCAGAGTACTTTTTTTTTTTTTT156580.47768636037344836No Hit
TTCCCATGTACTCTGCGTTGATACC153400.467984976889047No Hit
ACCATGTACTCTGCGTTGATACCAC148000.4515109294627051No Hit
TGATACCACTGCTTCCCATGTACTC129590.3953466307369726No Hit
ATCAACGCAGAGTACATGGGAAGCA126590.38619438216678265No Hit
GAGTACTTTTTTTTTTTTTTTTTTT110080.33582650753550386No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA104610.3191389076425241No Hit
CCCCATGTACTCTGCGTTGATACCA92970.28362818319018707No Hit
GGTAAGCAGTGGTATCAACGCAGAG84210.2569036173652324No Hit
GTACATGGGTAAGCAGTGGTATCAA84110.2565985424128927No Hit
GGAAGCAGTGGTATCAACGCAGAGT82080.25040552088039747No Hit
TCCATGTACTCTGCGTTGATACCAC79300.24192443720535478No Hit
CTGCGTTGATACCACTGCTTCCCAT65800.20073931863949993No Hit
GAGTACATGGTAAGCAGTGGTATCA65530.19991561626818283No Hit
AAAAAGTACTCTGCGTTGATACCAC65180.19884785393499402No Hit
CTCTGCGTTGATACCACTGCTTCCC60190.1836246138132447No Hit
TCAACGCAGAGTACATGGGAAGCAG58090.17721803981411172No Hit
GTACATGGAAAGCAGTGGTATCAAC56800.17328257292893004No Hit
TGGGAAGCAGTGGTATCAACGCAGA56140.17126907824348825No Hit
GCTTACCATGTACTCTGCGTTGATA53340.16272697957797763No Hit
TCCCATGTACTCTGCGTTGATACCA51310.1565339580454824No Hit
AGTGGTATCAACGCAGAGTACATGG49200.15009687655111545No Hit
AAAGTACTCTGCGTTGATACCACTG47830.14591734970406206No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA47380.14454451241853356No Hit
GCAGAGTACTTTTTTTTTTTTTTTT46870.14298863016160127No Hit
ACTCTGCGTTGATACCACTGCTTAC46420.14161579287607276No Hit
GTGGTATCAACGCAGAGTACTTTTT45010.1373142360480835No Hit
GCGTTGATACCACTGCTTACCATGT43520.13276861925822245No Hit
ACGCAGAGTACATGGTAAGCAGTGG43470.13261608178205261No Hit
AACGCAGAGTACATGGGAAGCAGTG43280.13203643937260726No Hit
GAGTACATGGAAAGCAGTGGTATCA43150.1316398419345657No Hit
ACTGCTTCCCATGTACTCTGCGTTG38910.11870466395536387No Hit
CTGCTTACCATGTACTCTGCGTTGA38410.11717928919366555No Hit
GTACATGGAAGCAGTGGTATCAACG38290.11681319925085794No Hit
ATCAACGCAGAGTACTTTTTTTTTT36950.11272519488950641No Hit
ATGGTAAGCAGTGGTATCAACGCAG36360.1109252526707024No Hit
CAACGCAGAGTACATGGGAAGCAGT35520.1083626230710492No Hit
TTGATACCACTGCTTCCCATGTACT33760.1029933039098711No Hit
TATCAACGCAGAGTACATGGTAAGC33660.10268822895753142No Hit
CGTTGATACCACTGCTTCCCATGTA33470.10210858654808608No Hit
GCTTTCCATGTACTCTGCGTTGATA32950.1005221967959198No Hit
GTATCAACGCAGAGTACATGGTAAG32940.10049168930068583No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGC250.00599296219.0221751
GTACCGA651.9167055E-716.0758026
TACCGAT651.9167055E-716.0758027
GTACAAA7700.015.9341611
GGACGCA2050.015.7549988
TAGACAG704.4438457E-714.9275295
GGGACGC2550.013.4108287
TGGGACG2550.013.4108286
CGATCAA1001.009721E-813.2990710
GTACATA4000.012.8399681
GAACAAA11700.012.6814511
TGTAGGA1155.331458E-912.3915732
TAGGACC853.9501683E-612.2932594
ACGCAGT3250.012.27606610
ACAGTCC701.0923243E-412.2134338
CGCAAAA3850.012.0911712
CGCAGTG3300.012.09006511
GACGCAG2750.012.0900649
AAAAAGT11250.012.089651
AAAAGTA11350.012.0531242
Produced by FastQC (version 0.11.5)