FastQCFastQC Report
Wed 25 May 2016
SRR1294994_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294994_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2179833
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC1728067.927488023165077No Hit
CCCATGTACTCTGCGTTGATACCAC1697797.788624174420701No Hit
GAGTACATGGGAAGCAGTGGTATCA799573.6680332851186304No Hit
CATGTACTCTGCGTTGATACCACTG641052.9408216133988243No Hit
GCGTTGATACCACTGCTTCCCATGT520062.3857790940865655No Hit
ACGCAGAGTACATGGGAAGCAGTGG453802.081810854317739No Hit
TATCAACGCAGAGTACATGGGAAGC443882.0363027809928558No Hit
GTATCAACGCAGAGTACATGGGAAG418761.9210645953153291No Hit
ACTCTGCGTTGATACCACTGCTTCC400501.8372967103443245No Hit
GGTATCAACGCAGAGTACATGGGAA384411.7634837164131378No Hit
GCTTCCCATGTACTCTGCGTTGATA280551.2870251987193513No Hit
GTGGTATCAACGCAGAGTACATGGG208250.9553484143051325No Hit
GTACTCTGCGTTGATACCACTGCTT206690.9481919027742033No Hit
ATACCACTGCTTCCCATGTACTCTG195400.8963989443227991No Hit
CAGTGGTATCAACGCAGAGTACATG181620.8331830924662578No Hit
ACATGGGAAGCAGTGGTATCAACGC178360.8182278183695723No Hit
GGGAAGCAGTGGTATCAACGCAGAG174920.8024467929423952No Hit
CATGGGAAGCAGTGGTATCAACGCA162710.7464333276906993No Hit
GCAGTGGTATCAACGCAGAGTACAT162710.7464333276906993No Hit
TACCACTGCTTCCCATGTACTCTGC161790.7422128208904076No Hit
GATACCACTGCTTCCCATGTACTCT160170.7347810589159812No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA158290.7261565450197331No Hit
GTTGATACCACTGCTTCCCATGTAC158070.7251472933935765No Hit
CTGCTTCCCATGTACTCTGCGTTGA155080.7114306462926289No Hit
CCATGTACTCTGCGTTGATACCACT145240.6662895735590755No Hit
GCAGAGTACATGGGAAGCAGTGGTA144950.6649591964155053No Hit
CTTCCCATGTACTCTGCGTTGATAC137720.6317915179740834No Hit
GTACATGGTAAGCAGTGGTATCAAC133500.6124322367814415No Hit
CCACTGCTTCCCATGTACTCTGCGT130400.5982109638674156No Hit
AAGCAGTGGTATCAACGCAGAGTAC122490.5619237803996912No Hit
TTCCCATGTACTCTGCGTTGATACC119390.5477025074856652No Hit
ATGGGAAGCAGTGGTATCAACGCAG112180.5146265791920757No Hit
ACCATGTACTCTGCGTTGATACCAC106400.48811078646850464No Hit
TGATACCACTGCTTCCCATGTACTC103820.4762750173981218No Hit
ATCAACGCAGAGTACATGGGAAGCA85010.38998400336172545No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA73610.3376864190972428No Hit
CCCCATGTACTCTGCGTTGATACCA62180.28525120961101147No Hit
GGTAAGCAGTGGTATCAACGCAGAG58900.27020418536649365No Hit
TCCATGTACTCTGCGTTGATACCAC55520.25469841038281377No Hit
GTATCAACGCAGAGTACTTTTTTTT55310.2537350338305733No Hit
GTACATGGGTAAGCAGTGGTATCAA54520.25011090299119243No Hit
GAACAAAAAAAAAAAAAAAAAAAAA51390.2357520048554178No Hit
GGAAGCAGTGGTATCAACGCAGAGT50690.23254074968128294No Hit
AAAAAGTACTCTGCGTTGATACCAC50070.22969649509847773No Hit
GAGTACATGGTAAGCAGTGGTATCA47210.21657622395844087No Hit
GTACTTTTTTTTTTTTTTTTTTTTT44140.20249257626616352No Hit
GCTTACCATGTACTCTGCGTTGATA41150.18877592916521588No Hit
GTACATGGAAAGCAGTGGTATCAAC41010.1881336781303889No Hit
GGTATCAACGCAGAGTACTTTTTTT40560.18606929980415932No Hit
TATCAACGCAGAGTACTTTTTTTTT40100.18395904640401353No Hit
CTCTGCGTTGATACCACTGCTTCCC39130.17950916423414087No Hit
CTGCGTTGATACCACTGCTTCCCAT38200.1752427823599331No Hit
TCCCATGTACTCTGCGTTGATACCA37910.1739124052163629No Hit
TGGGAAGCAGTGGTATCAACGCAGA37130.1703341494508983No Hit
TCAACGCAGAGTACATGGGAAGCAG36950.16950839812040647No Hit
AAAGTACTCTGCGTTGATACCACTG35460.16267301210689075No Hit
ACTCTGCGTTGATACCACTGCTTAC34150.15666337742386688No Hit
GCGTTGATACCACTGCTTACCATGT32500.14909399022769176No Hit
AGTGGTATCAACGCAGAGTACATGG31660.14524048401872985No Hit
ACGCAGAGTACATGGTAAGCAGTGG29220.13404696598317392No Hit
TATCAACGCAGAGTACATGGTAAGC28620.13129446154820115No Hit
GAGTACATGGAAAGCAGTGGTATCA27930.12812908144798246No Hit
TTGATACCACTGCTTCCCATGTACT27720.12716570489574203No Hit
CTGCTTACCATGTACTCTGCGTTGA27620.1267069541565799No Hit
GTATCAACGCAGAGTACATGGTAAG26740.12266994765195315No Hit
AACGCAGAGTACATGGGAAGCAGTG26640.12221119691279102No Hit
AAACAAAAAAAAAAAAAAAAAAAAA26200.12019269366047766No Hit
ATGGTAAGCAGTGGTATCAACGCAG25980.11918344203432098No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN25920.1189081915908237No Hit
GGTATCAACGCAGAGTACATGGTAA24420.11202693050339177No Hit
ACGCAGAGTACTTTTTTTTTTTTTT24380.11184343020772691No Hit
GTACATGGAAGCAGTGGTATCAACG23740.1089074254770893No Hit
ACTGCTTCCCATGTACTCTGCGTTG23720.10881567532925687No Hit
ATGTACTCTGCGTTGATACCACTGC22810.10464104360288151No Hit
GCTTTCCATGTACTCTGCGTTGATA22320.10239316498098706No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAA8550.017.129441
TACATAA7050.014.8300362
GTACATA9400.014.3664361
TCTGTCC400.005281991314.249198
TACATGA6000.013.9402342
CGCAAAA4950.013.6330122
AGAACAA11300.013.3738782
ACCTATG751.48009185E-512.6659457
TACAAAA12200.012.387282
TACAAGA2250.012.2505092
GCGTCTG701.0944479E-412.21050219
GAGAACA19100.011.7010021
CCCAGAC657.9924124E-411.6980992
GTAAAAA4800.011.689591
ATGTACG1001.9164345E-611.4069594
ACGCAAA6350.011.3822581
CGCAGAA4500.011.1944312
GTACAAG3500.011.1404921
GTAATAA600.0058302911.0952041
GTCTTAG600.0058302911.0952041
Produced by FastQC (version 0.11.5)