FastQCFastQC Report
Wed 25 May 2016
SRR1294994_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294994_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2179833
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC1894018.688784874804629No Hit
GTACATGGGAAGCAGTGGTATCAAC1692197.762934133027621No Hit
GAGTACATGGGAAGCAGTGGTATCA855353.923924447423266No Hit
CATGTACTCTGCGTTGATACCACTG709083.252909741250821No Hit
ACGCAGAGTACATGGGAAGCAGTGG560762.572490644925552No Hit
GCGTTGATACCACTGCTTCCCATGT558872.5638202559553873No Hit
TATCAACGCAGAGTACATGGGAAGC475282.1803505130897642No Hit
ACTCTGCGTTGATACCACTGCTTCC457812.1002067589581404No Hit
GTATCAACGCAGAGTACATGGGAAG443682.0353852795145317No Hit
GGTATCAACGCAGAGTACATGGGAA407291.8684458855334332No Hit
GCTTCCCATGTACTCTGCGTTGATA310951.4264854234246385No Hit
CAGTGGTATCAACGCAGAGTACATG220181.0100773774871745No Hit
ACATGGGAAGCAGTGGTATCAACGC219891.0087470003436043No Hit
GTGGTATCAACGCAGAGTACATGGG211840.971817565841053No Hit
ATACCACTGCTTCCCATGTACTCTG208810.9579174184444406No Hit
CATGGGAAGCAGTGGTATCAACGCA203930.9355303823733285No Hit
GGGAAGCAGTGGTATCAACGCAGAG198520.9107119673846574No Hit
GTACTCTGCGTTGATACCACTGCTT194140.8906186850093561No Hit
GCAGTGGTATCAACGCAGAGTACAT187650.8608457620377341No Hit
GTATCAACGCAGAGTACTTTTTTTT178550.8190994447739803No Hit
GATACCACTGCTTCCCATGTACTCT172810.7927671523460742No Hit
CTGCTTCCCATGTACTCTGCGTTGA170260.78106900849744No Hit
TACCACTGCTTCCCATGTACTCTGC169720.7785917545059645No Hit
CCATGTACTCTGCGTTGATACCACT169220.7762980008101538No Hit
GTACTTTTTTTTTTTTTTTTTTTTT168710.773958372040427No Hit
GCAGAGTACATGGGAAGCAGTGGTA161320.7400566924163456No Hit
CTTCCCATGTACTCTGCGTTGATAC156340.7172109056060716No Hit
GTTGATACCACTGCTTCCCATGTAC155340.7126233982144504No Hit
TATCAACGCAGAGTACTTTTTTTTT150970.6925759909130653No Hit
CCACTGCTTCCCATGTACTCTGCGT146370.6714734569116074No Hit
GGTATCAACGCAGAGTACTTTTTTT144850.6645004456763431No Hit
AAGCAGTGGTATCAACGCAGAGTAC140520.644636538670623No Hit
GTACATGGTAAGCAGTGGTATCAAC138240.6341770218177264No Hit
ATGGGAAGCAGTGGTATCAACGCAG130620.5992202154935723No Hit
ACCATGTACTCTGCGTTGATACCAC119080.5462803801942626No Hit
TTCCCATGTACTCTGCGTTGATACC118290.5426562493548818No Hit
ACGCAGAGTACTTTTTTTTTTTTTT100190.45962236556653646No Hit
TGATACCACTGCTTCCCATGTACTC98800.4532457302921829No Hit
ATCAACGCAGAGTACATGGGAAGCA95730.43916208259990563No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA92170.4228305562857338No Hit
GAGTACTTTTTTTTTTTTTTTTTTT80770.3705329720212512No Hit
CCCCATGTACTCTGCGTTGATACCA70600.3238780218484627No Hit
GGTAAGCAGTGGTATCAACGCAGAG67520.30974849908226915No Hit
GTACATGGGTAAGCAGTGGTATCAA57650.26446980112696705No Hit
GGAAGCAGTGGTATCAACGCAGAGT57620.2643321759052184No Hit
TCCATGTACTCTGCGTTGATACCAC57520.2638734251660563No Hit
GAGTACATGGTAAGCAGTGGTATCA51720.23726588229465284No Hit
AAAAAGTACTCTGCGTTGATACCAC48970.22465023696769432No Hit
GCTTACCATGTACTCTGCGTTGATA44820.20561208129246597No Hit
CTGCGTTGATACCACTGCTTCCCAT44810.2055662062185498No Hit
TCAACGCAGAGTACATGGGAAGCAG43080.19762981843104493No Hit
TGGGAAGCAGTGGTATCAACGCAGA43030.19740044306146387No Hit
CTCTGCGTTGATACCACTGCTTCCC42840.19652881665705585No Hit
GTACATGGAAAGCAGTGGTATCAAC40350.18510592325191882No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA40320.1849682980301702No Hit
TCCCATGTACTCTGCGTTGATACCA38360.17597678354259247No Hit
AAAGTACTCTGCGTTGATACCACTG37940.17405003043811154No Hit
ACTCTGCGTTGATACCACTGCTTAC37930.17400415536419533No Hit
ACGCAGAGTACATGGTAAGCAGTGG36690.16831564619858494No Hit
GCGTTGATACCACTGCTTACCATGT34770.15950763200667206No Hit
AGTGGTATCAACGCAGAGTACATGG33520.15377324776714546No Hit
AACGCAGAGTACATGGGAAGCAGTG32330.14831411397111613No Hit
GAGTACATGGAAAGCAGTGGTATCA32150.14748836264062432No Hit
GCAGAGTACTTTTTTTTTTTTTTTT31970.14666261131013247No Hit
CTGCTTACCATGTACTCTGCGTTGA29870.13702884578772775No Hit
TATCAACGCAGAGTACATGGTAAGC29240.13413871613100636No Hit
ATGGTAAGCAGTGGTATCAACGCAG29170.13381759061359286No Hit
GTGGTATCAACGCAGAGTACTTTTT28400.1302852099220445No Hit
CAACGCAGAGTACATGGGAAGCAGT26620.1221194467649586No Hit
TTGATACCACTGCTTCCCATGTACT26080.1196421927734831No Hit
GTATCAACGCAGAGTACATGGTAAG26050.11950456755173446No Hit
ACTGCTTCCCATGTACTCTGCGTTG25450.11675206311676169No Hit
GGTATCAACGCAGAGTACATGGTAA24280.1113846794685648No Hit
ATCAACGCAGAGTACTTTTTTTTTT23890.10959555158583248No Hit
GTACATGGAAGCAGTGGTATCAACG23790.10913680084667035No Hit
GCTTTCCATGTACTCTGCGTTGATA23620.10835692459009476No Hit
CGTTGATACCACTGCTTCCCATGTA23380.10725592281610563No Hit
GAACAAAAAAAAAAAAAAAAAAAAA23140.10615492104211653No Hit
CGCAGAGTACATGGGAAGCAGTGGT23080.10587967059861926No Hit
ATGTACTCTGCGTTGATACCACTGC22820.10468691867679772No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAA5350.017.06621
GGACGCA1850.016.9450668
GTCCTGC402.7628502E-416.6241386
GGGACGC1950.016.0760887
GATACGA508.720364E-515.19921118
GAACAAA8000.014.5040481
TGGGACG2200.014.2492616
GTACAAG1550.014.1128771
TGATACG551.9619672E-413.81714717
ACGCAGT2650.013.62162210
AAGCCAT500.001500471813.299316
CGCAGTG2850.012.99902811
ATACGAC604.0949948E-412.66600919
TACAAAA7250.012.447632
ACAGGGT853.9476636E-612.2934793
CGCCGAG701.0918184E-412.2136528
AACAGTC550.00307032112.09028157
GACGCAG2600.012.0567889
AAAAGTA8150.012.0055122
GCCCAAG657.949202E-411.7056631
Produced by FastQC (version 0.11.5)