FastQCFastQC Report
Wed 25 May 2016
SRR1294993_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294993_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2746762
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC137280.4997884782154406No Hit
CCCATGTACTCTGCGTTGATACCAC118150.4301428372753081No Hit
GAGTACATGGGAAGCAGTGGTATCA65080.23693352390924297No Hit
GTATCAACGCAGAGTACTTTTTTTT55750.2029662562682897No Hit
GGTATCAACGCAGAGTACTTTTTTT48080.17504246818617702No Hit
CATGTACTCTGCGTTGATACCACTG46410.16896258212396997No Hit
GCTTCCCATGTACTCTGCGTTGATA40440.14722789961416388No Hit
GTATCAACGCAGAGTACATGGGAAG38370.13969175341729645No Hit
GCGTTGATACCACTGCTTCCCATGT36960.1345584364426186No Hit
TATCAACGCAGAGTACTTTTTTTTT36150.13160950966993137No Hit
GGTATCAACGCAGAGTACATGGGAA35720.13004403002517145No Hit
ACGCAGAGTACATGGGAAGCAGTGG35010.12745916828614928No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN33600.12232585131147146No Hit
TATCAACGCAGAGTACATGGGAAGC31610.1150809571415361No Hit
ATTCCATTCCATTCCATTCCATTCC28710.1045230711652484No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCCG700.001487434610.861165
CTGTCGC2300.010.3253839
GTCTAGG2400.010.3024331
GTGTTAG3900.09.5099391
TCAGGAC3300.09.5026473
CCTATAC2600.09.5026473
TCTGTCG2303.6379788E-129.4993528
CTAGGAC1856.670234E-99.2458193
TAAGGGT1651.06005245E-79.2156984
GTCCTAG2750.08.9912151
ATAGGAC2553.6379788E-128.9436673
CCGTCCC850.00744194448.9405679
ACACCGT850.00744640138.9399146
TCTATAC3550.08.83344653
GTCCAGG4250.08.7267681
GCCTAAC1201.744586E-48.7174431
TAGGACA2850.08.6700324
GTCGCCC2201.6116246E-98.63467211
GTCTTGC5550.08.56751251
GTCTTAC2901.8189894E-128.5261521
Produced by FastQC (version 0.11.5)