##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294993_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2746762 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.049814290426326 33.0 33.0 33.0 27.0 33.0 2 30.96282859599776 33.0 33.0 33.0 27.0 33.0 3 30.984645557205173 33.0 33.0 33.0 27.0 33.0 4 34.54349666989714 37.0 37.0 37.0 27.0 37.0 5 34.70991225304559 37.0 37.0 37.0 27.0 37.0 6 34.83081060536006 37.0 37.0 37.0 33.0 37.0 7 34.652270928460496 37.0 37.0 37.0 27.0 37.0 8 34.57636955804689 37.0 37.0 37.0 27.0 37.0 9 34.55610132949269 37.0 37.0 37.0 27.0 37.0 10 34.45910603102853 37.0 37.0 37.0 27.0 37.0 11 34.640874600711676 37.0 37.0 37.0 27.0 37.0 12 34.54482150255465 37.0 37.0 37.0 27.0 37.0 13 34.46812064532712 37.0 37.0 37.0 27.0 37.0 14 35.81454672811114 40.0 37.0 40.0 27.0 40.0 15 35.991167782283284 40.0 37.0 40.0 27.0 40.0 16 36.061786933123436 40.0 37.0 40.0 27.0 40.0 17 35.975005115113724 40.0 37.0 40.0 27.0 40.0 18 35.9130539886601 37.0 37.0 40.0 27.0 40.0 19 36.02295648476279 40.0 37.0 40.0 27.0 40.0 20 36.054968723172955 40.0 37.0 40.0 27.0 40.0 21 36.04708380267384 40.0 37.0 40.0 27.0 40.0 22 35.93839255093816 40.0 37.0 40.0 27.0 40.0 23 35.83526093633158 37.0 37.0 40.0 27.0 40.0 24 35.91029656009512 37.0 37.0 40.0 27.0 40.0 25 34.75368269984804 37.0 33.0 40.0 22.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5739918409806748 1101 2 0.5863653751795397 1101 3 0.6356732310644126 1101 4 0.6278174096939395 1101 5 0.41865828293961727 1101 6 0.5081831832447818 1101 7 0.5381129720353286 1101 8 0.6267020876214531 1101 9 0.5950777716255686 1101 10 0.7106144400926979 1101 11 0.6780174208916776 1101 12 0.7304007202682214 1101 13 0.6604498561363172 1101 14 1.15815161800937 1101 15 1.0490458258213238 1101 16 0.9987686879391546 1101 17 0.8740558554946602 1101 18 0.9431886350753658 1101 19 0.864325810254762 1101 20 0.8912498355452456 1101 21 0.9374109817884673 1101 22 0.9565850865908487 1101 23 0.9886782072158837 1101 24 0.9533907112686393 1101 25 1.1415140981094964 1102 1 0.41686906030057 1102 2 0.346941177002023 1102 3 0.37965035094124744 1102 4 0.5155440901127903 1102 5 0.20979241784431224 1102 6 0.23353093719214257 1102 7 0.2821675278334794 1102 8 0.35385351411348864 1102 9 0.30480903793117164 1102 10 0.4448852229168949 1102 11 0.41011598001738037 1102 12 0.48165060726473996 1102 13 0.4469102583465272 1102 14 0.7806911327016479 1102 15 0.6654637021959715 1102 16 0.6460929937781685 1102 17 0.496716820393722 1102 18 0.7123771741937475 1102 19 0.6602953070765096 1102 20 0.668980250786241 1102 21 0.5808866858115707 1102 22 0.5783224820226778 1102 23 0.6140545430540101 1102 24 0.6790924058186292 1102 25 0.6434062558048268 1103 1 0.28317296861538566 1103 2 0.30753769318093305 1103 3 0.3085082406239046 1103 4 0.253495205547253 1103 5 0.0031954252261527927 1103 6 0.1271382557261873 1103 7 0.17436457432546604 1103 8 0.37802589374953754 1103 9 0.3707596810796545 1103 10 0.5329819252512422 1103 11 0.28529886475319444 1103 12 0.48254148339379554 1103 13 0.4641834060702834 1103 14 0.6731519117539904 1103 15 0.6038426636355396 1103 16 0.5051855817065771 1103 17 0.5397095589967833 1103 18 0.5196148975023931 1103 19 0.5836478018115017 1103 20 0.5569251214846034 1103 21 0.6730466495476009 1103 22 0.5262968587282941 1103 23 0.6117349425035243 1103 24 0.6568070934676058 1103 25 0.5586642672375888 1104 1 0.27132858176183916 1104 2 0.12793383960078586 1104 3 0.2856526775224353 1104 4 0.34071307432888176 1104 5 0.08057937309375518 1104 6 0.17048329646829785 1104 7 0.20427546819497167 1104 8 0.2812040294656697 1104 9 0.3700517574300122 1104 10 0.4228403242351746 1104 11 0.18238522759957476 1104 12 0.35847005517135955 1104 13 0.3497818158914825 1104 14 0.5937848353978126 1104 15 0.5743598044499407 1104 16 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2214 15 -0.23452163000522575 2214 16 -0.317821287605625 2214 17 -0.3273395104727044 2214 18 -0.28259437842535107 2214 19 -0.9043053186409225 2214 20 -0.5092983689412947 2214 21 -0.3529856076400151 2214 22 -0.6677317442862503 2214 23 -1.8439683897777925 2214 24 -1.472575676018998 2214 25 -1.688368107816423 2215 1 -0.37603506306561485 2215 2 -0.15177604835254854 2215 3 -1.1699000710258538 2215 4 -0.7774025834366043 2215 5 -0.20939971557385206 2215 6 -0.21189024959782898 2215 7 -0.12448310581744693 2215 8 -0.08491223712400142 2215 9 -0.26389536901398003 2215 10 -1.234734306416719 2215 11 -0.46872430740310733 2215 12 -0.42463068012506966 2215 13 -0.5407525894745433 2215 14 -0.8003314855885293 2215 15 -0.9046276539810734 2215 16 -0.6135226140025125 2215 17 -0.4407440140967367 2215 18 -0.3623124028787714 2215 19 -0.9556896896488567 2215 20 -1.2358380572637486 2215 21 -0.6423022303325538 2215 22 -0.3324459155961179 2215 23 -0.47603820033904043 2215 24 -1.5933288063147089 2215 25 -0.7061321614083909 2216 1 -0.4648598648968445 2216 2 -0.4291496895276268 2216 3 -1.2896739002490527 2216 4 -0.6922922917194896 2216 5 -0.4288545812257851 2216 6 -0.3970264259153353 2216 7 -0.21880001354514178 2216 8 -0.3503254206406865 2216 9 -0.10017475852917812 2216 10 -0.37900452858588096 2216 11 -0.36334618702618826 2216 12 -0.3200388452940075 2216 13 -0.8924663832148525 2216 14 -0.610521856330628 2216 15 -0.8744818887016947 2216 16 -0.7370878609182796 2216 17 -1.631776282632437 2216 18 -0.6643797662687163 2216 19 -0.5034386155545363 2216 20 -0.41702881417609916 2216 21 -0.23794125815360445 2216 22 -0.25706287014928364 2216 23 -0.1599621844967274 2216 24 -0.24062318930978677 2216 25 -0.13570100325208045 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3685.0 3 366.0 4 457.0 5 421.0 6 292.0 7 229.0 8 406.0 9 875.0 10 1833.0 11 3303.0 12 5246.0 13 7725.0 14 10190.0 15 12918.0 16 14354.0 17 15239.0 18 16004.0 19 16219.0 20 16964.0 21 17758.0 22 18870.0 23 19947.0 24 21253.0 25 22328.0 26 23171.0 27 25916.0 28 29077.0 29 34120.0 30 41172.0 31 51906.0 32 69424.0 33 97673.0 34 141027.0 35 223838.0 36 410487.0 37 1372069.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.33024111420686 21.24362209262195 13.661677555353718 22.764459237817473 2 14.83008392308795 21.61107964213144 40.61098371544132 22.947852719339295 3 18.509894420120514 26.676156282444236 29.867009187806108 24.94694010962914 4 11.792136313531632 18.15185817239365 38.64165191637084 31.41435359770388 5 12.395044011623536 39.0212550703332 35.14787452946359 13.435826388579681 6 30.2220401486761 38.85241135375289 17.86757992033593 13.057968577235076 7 25.173278820861377 33.49259554729698 23.37368146316961 17.960444168672034 8 23.73326853459253 37.829439206302986 21.226221306672446 17.211070952432035 9 23.732148649333997 17.718340065653347 22.386171129968012 36.163340155044644 10 14.316344887228198 31.128009954424208 34.00399198683447 20.551653171513124 11 31.316996792617473 26.530474511669443 23.04769923565652 19.10482946005656 12 20.265355903214815 28.780976855875124 31.782511333463216 19.17115590744685 13 26.170152678106213 24.601921867223613 26.683275756129525 22.54464969854065 14 19.819524667053876 24.630680076935874 28.536430712474058 27.013364543536188 15 22.979605446038303 31.12677484713022 24.22510836407996 21.66851134275152 16 20.7363137760779 32.27957684357528 26.56664407244646 20.417465307900365 17 19.647176249898767 31.23289490292732 28.8430880268151 20.276840820358814 18 19.729052142176485 30.563391175041332 29.90699893909036 19.80055774369182 19 20.25182118528809 29.732528003642372 30.28804059141825 19.72761021965129 20 21.576747591463114 29.271151512100413 29.335320793510256 19.816780102926217 21 21.56060191518468 29.690323757409942 28.73200182398541 20.01707250341997 22 20.424012438593202 29.46738334988359 29.512546942045407 20.596057269477804 23 20.165154784727836 29.994035165163908 29.2894550110997 20.55135503900856 24 20.431318303406417 29.50542960883683 29.42833029448435 20.6349217932724 25 21.062482828724345 29.066221925306362 28.313367525683486 21.557927720285804 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 6499.0 1 6499.0 2 5474.0 3 4449.0 4 4449.0 5 4449.0 6 6162.5 7 7876.0 8 7876.0 9 7876.0 10 11263.0 11 14650.0 12 14650.0 13 14650.0 14 22930.0 15 31210.0 16 31210.0 17 31210.0 18 48104.5 19 64999.0 20 64999.0 21 64999.0 22 90638.5 23 116278.0 24 116278.0 25 116278.0 26 148341.0 27 180404.0 28 180404.0 29 180404.0 30 212211.0 31 244018.0 32 244018.0 33 244018.0 34 268143.0 35 292268.0 36 292268.0 37 292268.0 38 305805.0 39 319342.0 40 319342.0 41 319342.0 42 315473.5 43 311605.0 44 311605.0 45 311605.0 46 324056.0 47 336507.0 48 336507.0 49 336507.0 50 308238.5 51 279970.0 52 279970.0 53 279970.0 54 245769.0 55 211568.0 56 211568.0 57 211568.0 58 180460.5 59 149353.0 60 149353.0 61 149353.0 62 121740.5 63 94128.0 64 94128.0 65 94128.0 66 71188.5 67 48249.0 68 48249.0 69 48249.0 70 34409.0 71 20569.0 72 20569.0 73 20569.0 74 14320.0 75 8071.0 76 8071.0 77 8071.0 78 5522.5 79 2974.0 80 2974.0 81 2974.0 82 2030.0 83 1086.0 84 1086.0 85 1086.0 86 783.0 87 480.0 88 480.0 89 480.0 90 314.0 91 148.0 92 148.0 93 148.0 94 96.5 95 45.0 96 45.0 97 45.0 98 30.5 99 16.0 100 16.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.31305952244861407 2 0.18512706961870012 3 0.20096389858313168 4 0.223499524167001 5 0.24770984890572972 6 0.20027217501916803 7 0.1955393295815218 8 0.21727401209132788 9 0.21854823970915574 10 0.22834158911474675 11 0.2033667278053213 12 0.20984708540455999 13 0.22186123151550807 14 0.21035677645169112 15 0.20169202865046187 16 0.2056239310140449 17 0.20460454891978266 18 0.20817238624970055 19 0.2071530041554383 20 0.2029662562682897 21 0.20067264655619965 22 0.2044589229063166 23 0.20740784967900383 24 0.22357233717373404 25 0.21818417467549064 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2746762.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.7547316883061 #Duplication Level Percentage of deduplicated Percentage of total 1 92.50462134267399 76.55195119141341 2 6.0412852985989485 9.998898878761283 3 0.7573713397589503 1.8802818603049456 4 0.2231484520080974 0.7386636109036381 5 0.11218202633848376 0.4641796744945856 6 0.06471306028982282 0.32131871646080784 7 0.04272773341865917 0.24751454802974265 8 0.03602987941073414 0.23853144027178624 9 0.027130001717216645 0.20206224115303922 >10 0.1642999329800269 2.500471678374142 >50 0.014140507588873643 0.8235355397041587 >100 0.009871485782705433 1.5004841470620638 >500 9.296022876172511E-4 0.5726447326787463 >1k 0.0013722700434397202 2.5811140747187005 >5k 8.853355118965937E-5 0.4426347431105474 >10k+ 8.853355118965937E-5 0.935712922558364 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 13728 0.4997884782154406 No Hit CCCATGTACTCTGCGTTGATACCAC 11815 0.4301428372753081 No Hit GAGTACATGGGAAGCAGTGGTATCA 6508 0.23693352390924297 No Hit GTATCAACGCAGAGTACTTTTTTTT 5575 0.2029662562682897 No Hit GGTATCAACGCAGAGTACTTTTTTT 4808 0.17504246818617702 No Hit CATGTACTCTGCGTTGATACCACTG 4641 0.16896258212396997 No Hit GCTTCCCATGTACTCTGCGTTGATA 4044 0.14722789961416388 No Hit GTATCAACGCAGAGTACATGGGAAG 3837 0.13969175341729645 No Hit GCGTTGATACCACTGCTTCCCATGT 3696 0.1345584364426186 No Hit TATCAACGCAGAGTACTTTTTTTTT 3615 0.13160950966993137 No Hit GGTATCAACGCAGAGTACATGGGAA 3572 0.13004403002517145 No Hit ACGCAGAGTACATGGGAAGCAGTGG 3501 0.12745916828614928 No Hit NNNNNNNNNNNNNNNNNNNNNNNNN 3360 0.12232585131147146 No Hit TATCAACGCAGAGTACATGGGAAGC 3161 0.1150809571415361 No Hit ATTCCATTCCATTCCATTCCATTCC 2871 0.1045230711652484 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.640650336650937E-5 0.0 5 0.0 0.0 0.0 3.640650336650937E-5 0.0 6 0.0 0.0 0.0 3.640650336650937E-5 0.0 7 0.0 0.0 0.0 3.640650336650937E-5 0.0 8 0.0 0.0 0.0 3.640650336650937E-5 0.0 9 0.0 0.0 0.0 7.281300673301873E-5 0.0 10 0.0 0.0 0.0 7.281300673301873E-5 0.0 11 0.0 0.0 0.0 7.281300673301873E-5 0.0 12 0.0 0.0 0.0 1.092195100995281E-4 0.0 13 0.0 0.0 0.0 1.092195100995281E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATTCCG 70 0.0014874346 10.86116 5 CTGTCGC 230 0.0 10.325383 9 GTCTAGG 240 0.0 10.302433 1 GTGTTAG 390 0.0 9.509939 1 TCAGGAC 330 0.0 9.502647 3 CCTATAC 260 0.0 9.502647 3 TCTGTCG 230 3.6379788E-12 9.499352 8 CTAGGAC 185 6.670234E-9 9.245819 3 TAAGGGT 165 1.06005245E-7 9.215698 4 GTCCTAG 275 0.0 8.991215 1 ATAGGAC 255 3.6379788E-12 8.943667 3 CCGTCCC 85 0.0074419444 8.940567 9 ACACCGT 85 0.0074464013 8.939914 6 TCTATAC 355 0.0 8.8334465 3 GTCCAGG 425 0.0 8.726768 1 GCCTAAC 120 1.744586E-4 8.717443 1 TAGGACA 285 0.0 8.670032 4 GTCGCCC 220 1.6116246E-9 8.634672 11 GTCTTGC 555 0.0 8.5675125 1 GTCTTAC 290 1.8189894E-12 8.526152 1 >>END_MODULE