FastQCFastQC Report
Wed 25 May 2016
SRR1294993_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294993_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2746762
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC135730.4941454701936316No Hit
CCCATGTACTCTGCGTTGATACCAC134900.4911237304142113No Hit
GTATCAACGCAGAGTACTTTTTTTT130530.47521408844304674No Hit
GGTATCAACGCAGAGTACTTTTTTT119440.4348392762095879No Hit
TATCAACGCAGAGTACTTTTTTTTT86680.3155715711809032No Hit
GAGTACATGGGAAGCAGTGGTATCA70680.2573211657944882No Hit
CATGTACTCTGCGTTGATACCACTG51600.18785755737118834No Hit
ACGCAGAGTACTTTTTTTTTTTTTT51040.1858187931826638No Hit
GCTTCCCATGTACTCTGCGTTGATA45340.16506708626375347No Hit
ACGCAGAGTACATGGGAAGCAGTGG44990.16379285864592563No Hit
GCGTTGATACCACTGCTTCCCATGT40450.14726430611753039No Hit
GGTATCAACGCAGAGTACATGGGAA40120.14606289150643556No Hit
GTATCAACGCAGAGTACATGGGAAG38450.13998300544422854No Hit
TATCAACGCAGAGTACATGGGAAGC33970.12367289193603231No Hit
ACTCTGCGTTGATACCACTGCTTCC30610.11144030680488516No Hit
GTGGTATCAACGCAGAGTACATGGG29420.10710793290427056No Hit
ATTCCATTCCATTCCATTCCATTCC28050.10212024194305876No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTACAC2500.09.8792433
TCTAGAC2850.09.6659263
CACACCG800.0045201679.4992725
TATTCCG1201.7028908E-59.4992715
TAGGACT3450.09.3616014
GTCCTAT2451.8189894E-129.318811
GTTCTAG3400.09.2331591
GTCTAAC1451.6760005E-69.1849191
ACTATCC1451.7090788E-69.1717118
CGCCTCC2401.0913936E-119.10346911
CTAGGAC2106.693881E-109.0469263
TTACACC1804.2269676E-88.9715354
AGACGTG850.0074424668.9404925
TTTACAC4150.08.9270273
TACACTG6250.08.8153255
CCAGGAC4100.08.8042023
CTACACT4650.08.7842734
CTAGGTA2607.2759576E-128.76855854
GACTGTG4800.08.7076657
TTAGGCT2850.08.6660024
Produced by FastQC (version 0.11.5)