FastQCFastQC Report
Wed 25 May 2016
SRR1294992_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294992_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1028611
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC80850.7860114270603756No Hit
CCCATGTACTCTGCGTTGATACCAC75800.7369160936447307No Hit
GAGTACATGGGAAGCAGTGGTATCA36790.3576667953191245No Hit
CATGTACTCTGCGTTGATACCACTG27930.27153122025722065No Hit
GCGTTGATACCACTGCTTCCCATGT24640.239546339675543No Hit
GCTTCCCATGTACTCTGCGTTGATA22930.22292197925163157No Hit
ACGCAGAGTACATGGGAAGCAGTGG22530.21903323997118446No Hit
GTATCAACGCAGAGTACATGGGAAG22070.21456118979867023No Hit
GGTATCAACGCAGAGTACATGGGAA21580.20979748418012253No Hit
TATCAACGCAGAGTACATGGGAAGC19900.19346477920224459No Hit
ACTCTGCGTTGATACCACTGCTTCC18710.18189577984291438No Hit
GGTATCAACGCAGAGTACTTTTTTT17760.17266002405185246No Hit
GTGGTATCAACGCAGAGTACATGGG17720.17227115012380773No Hit
GTATCAACGCAGAGTACTTTTTTTT17170.16692413361319292No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN12340.11996760680179387No Hit
TATCAACGCAGAGTACTTTTTTTTT11520.11199569127687728No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAG902.242814E-914.7971891
ATAGGGG400.00528449514.24714858
CCTATAC1009.95351E-913.3096693
TTAGACT751.4620111E-512.6795884
GTCTCGC701.0769229E-412.2303291
GTAGGAC550.0030505712.0996993
GTACAAG1459.458745E-1111.8085931
CTAGCCT1001.9037816E-611.411634
CTTAGAC600.005841656611.0913923
GTCTAAC951.3392024E-511.0144481
GTATTAA1752.0008883E-1110.8714041
TAAGGGT803.73297E-410.6984034
TAGGACA1451.4171746E-810.4934534
TAGACAG1855.820766E-1110.2807475
CCAGGAC1502.4114343E-810.14073
TCTCGCT750.002631414210.1402052
AGGACTG1803.8562575E-1010.0380085
TCTAGCC951.63058E-410.007273
GTACTAT1151.0091766E-59.92606451
CCTAGAC1151.0162299E-59.9202513
Produced by FastQC (version 0.11.5)