##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294992_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1028611
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.112320401006794	33.0	33.0	33.0	27.0	33.0
2	31.087271086931793	33.0	33.0	33.0	27.0	33.0
3	31.164767827682184	33.0	33.0	33.0	27.0	33.0
4	34.66146191320139	37.0	37.0	37.0	27.0	37.0
5	35.04674070178133	37.0	37.0	37.0	33.0	37.0
6	35.08418828886722	37.0	37.0	37.0	33.0	37.0
7	34.92229715606774	37.0	37.0	37.0	33.0	37.0
8	34.84015337187722	37.0	37.0	37.0	33.0	37.0
9	34.786776536513806	37.0	37.0	37.0	33.0	37.0
10	34.70409513411776	37.0	37.0	37.0	27.0	37.0
11	34.884389725561945	37.0	37.0	37.0	33.0	37.0
12	34.80038517962573	37.0	37.0	37.0	33.0	37.0
13	34.72231971075557	37.0	37.0	37.0	27.0	37.0
14	36.157717543366736	40.0	37.0	40.0	27.0	40.0
15	36.3202318466359	40.0	37.0	40.0	27.0	40.0
16	36.39320015049421	40.0	37.0	40.0	27.0	40.0
17	36.32136735850579	40.0	37.0	40.0	27.0	40.0
18	36.25670345738087	40.0	37.0	40.0	27.0	40.0
19	36.339194311552184	40.0	37.0	40.0	27.0	40.0
20	36.34622126343195	40.0	37.0	40.0	27.0	40.0
21	36.35711264997166	40.0	37.0	40.0	27.0	40.0
22	36.27470054277079	40.0	37.0	40.0	27.0	40.0
23	36.16856518158954	40.0	37.0	40.0	27.0	40.0
24	36.22081622693127	40.0	37.0	40.0	27.0	40.0
25	35.17002443100453	37.0	33.0	40.0	27.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.23126398913782964
1101	2	0.19924989102590374
1101	3	0.27885806456783513
1101	4	0.20004182121238046
1101	5	0.2930938554281539
1101	6	0.08886951428358003
1101	7	0.16675955914362817
1101	8	0.37037628624987917
1101	9	0.244322820023271
1101	10	0.45030335057889204
1101	11	0.3125394442251306
1101	12	0.2633464432738464
1101	13	0.3003758595199031
1101	14	0.7087626876903528
1101	15	0.6001663812142866
1101	16	0.462822589738181
1101	17	0.5586346498812347
1101	18	0.49710800290043977
1101	19	0.5076160277671207
1101	20	0.5093809289499731
1101	21	0.4046920416704012
1101	22	0.5763056091925591
1101	23	0.4889439931708921
1101	24	0.4632817075810678
1101	25	0.46918468196908947
1102	1	0.1615440497777385
1102	2	0.26221167192433015
1102	3	0.33005002889312607
1102	4	0.3964415481554724
1102	5	0.2052448878137625
1102	6	0.4222019610685095
1102	7	0.31657382725359895
1102	8	0.4364321210681794
1102	9	0.36695196796160445
1102	10	0.552612809163783
1102	11	0.39726598837081184
1102	12	0.5843071071212833
1102	13	0.49087383375680815
1102	14	0.8506973136112563
1102	15	0.8102934236001644
1102	16	0.828438696549533
1102	17	0.8317296790043542
1102	18	0.889110449774023
1102	19	0.8903584765911177
1102	20	0.7918565267052315
1102	21	0.7625012032615714
1102	22	0.7396025053866069
1102	23	0.7307252462184053
1102	24	0.9428574055162997
1102	25	0.9083018570712582
1103	1	0.08786169822167622
1103	2	0.2611952282996697
1103	3	0.2705917833158118
1103	4	0.2081635719727828
1103	5	0.024044952349868254
1103	6	0.029791057955513622
1103	7	-0.1686460969600887
1103	8	0.22786059282709914
1103	9	0.20791407916428284
1103	10	0.35880260635472183
1103	11	0.24830750514365718
1103	12	0.20252067801725104
1103	13	0.1935721405562063
1103	14	0.4840371877658498
1103	15	0.5035358831233836
1103	16	0.40794630352466044
1103	17	0.27951822733447784
1103	18	0.4399300146785947
1103	19	0.6264502922794932
1103	20	0.5504858322008275
1103	21	0.3951573435665523
1103	22	0.625933497105926
1103	23	0.5185641152668055
1103	24	0.5571272972439019
1103	25	0.4625060789533606
1104	1	0.12490317953185226
1104	2	0.07157504638668044
1104	3	0.1428556160553498
1104	4	0.3074246186378957
1104	5	0.029271514526016063
1104	6	0.20620782152754202
1104	7	0.3060337881061628
1104	8	0.44801719769978376
1104	9	0.5640036262581063
1104	10	0.7075226920532387
1104	11	0.4872248414208755
1104	12	0.5819063453333442
1104	13	0.7448780174933134
1104	14	0.8553993098076944
1104	15	0.8793779779371889
1104	16	0.7783750797245617
1104	17	0.8157958835411847
1104	18	1.004437128222584
1104	19	0.7404899692955809
1104	20	0.9172139510442463
1104	21	0.767955482983723
1104	22	0.6652797623044293
1104	23	0.7534600792769197
1104	24	0.9141885886995809
1104	25	0.9927822210366131
1105	1	0.036272535705887066
1105	2	-0.07320782708337248
1105	3	0.022722144072389483
1105	4	0.007537032557415557
1105	5	0.024282386706872217
1105	6	-0.10762682228800458
1105	7	-0.09111659721991572
1105	8	0.26961439621285876
1105	9	0.07154986669010555
1105	10	0.25010836857392604
1105	11	0.10815363754077367
1105	12	0.23219828271862042
1105	13	0.11606460983126965
1105	14	0.5926143982923904
1105	15	0.30778012787511244
1105	16	0.3016935838028232
1105	17	0.26290965181387804
1105	18	0.18562725629973897
1105	19	0.3048699596179034
1105	20	0.3154528575539146
1105	21	0.3371604107790631
1105	22	0.24690799192419632
1105	23	0.5411862862279904
1105	24	0.4665422877169547
1105	25	0.398444008035689
1106	1	-0.1739985381103466
1106	2	-0.2704031296619718
1106	3	-0.2715030169716144
1106	4	-0.26147753248162076
1106	5	-0.4061051749285056
1106	6	-0.411700105094539
1106	7	-0.795442784775588
1106	8	-0.1732396907441327
1106	9	0.020600661552947486
1106	10	-0.3868541749871568
1106	11	-0.46453752351948197
1106	12	-0.7443687654713784
1106	13	-0.3170523735078845
1106	14	-0.3447483521415293
1106	15	-0.20977292390536917
1106	16	-0.03966419518442166
1106	17	-0.1905263597645046
1106	18	-0.5427506061406575
1106	19	-0.6447562341656194
1106	20	-0.11973556189970935
1106	21	0.04723652590217142
1106	22	0.134912346001677
1106	23	-0.3418168358188609
1106	24	-0.11611613652940633
1106	25	0.10131748255160744
1107	1	-1.135536609782605
1107	2	-1.0444754947283457
1107	3	-0.735873364914319
1107	4	-0.741181352143208
1107	5	-0.9626455890186207
1107	6	-0.7451594838668143
1107	7	-0.8451844577335095
1107	8	-0.7283583475404711
1107	9	-0.7722758319863416
1107	10	-0.5802805968436076
1107	11	-0.5750128250038671
1107	12	-0.8129894890162745
1107	13	-0.5904440491321523
1107	14	-0.7317642616320867
1107	15	-0.8566954099268926
1107	16	-0.7989689708521865
1107	17	-0.8730972283718259
1107	18	-1.148591974870719
1107	19	-1.013338218570361
1107	20	-0.851261861985698
1107	21	-1.0913147360286217
1107	22	-1.1147814649469154
1107	23	-1.0591189521922928
1107	24	-0.8150804044103381
1107	25	-0.6376009858817184
1108	1	0.2009199018097192
1108	2	-0.07449294470733392
1108	3	0.08225640792607791
1108	4	-0.11106861482058861
1108	5	-0.305652524465323
1108	6	0.0335623874836557
1108	7	0.11462597468123192
1108	8	0.2854784593264128
1108	9	0.026318486021608578
1108	10	-0.5510839349994114
1108	11	-0.07766247164663298
1108	12	0.1808768724210168
1108	13	0.2748599310511821
1108	14	-0.17060624926410384
1108	15	-0.039979840710898884
1108	16	-0.63759055808368
1108	17	-0.7002146119208206
1108	18	-0.5804706268259494
1108	19	-0.08684698132618252
1108	20	-0.8511816811721502
1108	21	-0.32813903352655416
1108	22	-0.23293351023551878
1108	23	-0.14204411870305478
1108	24	-0.35857462228911174
1108	25	-1.1401475982423506
1109	1	0.1663029768559987
1109	2	0.09572920615297775
1109	3	0.31945564976728846
1109	4	0.30233831971162317
1109	5	0.3493029143273816
1109	6	0.27562248282562507
1109	7	0.34796392193123893
1109	8	0.5792609297650486
1109	9	0.4343662640516115
1109	10	0.51476477651822
1109	11	0.5449446127455957
1109	12	0.47703311071320087
1109	13	0.38694857730957466
1109	14	0.615770884903057
1109	15	0.40392766686976955
1109	16	0.31693098798443486
1109	17	0.43742221377832635
1109	18	0.5410204433997023
1109	19	0.6313663624899704
1109	20	0.5179534309183396
1109	21	0.46054099873394705
1109	22	0.5121483314185511
1109	23	0.6162254041625914
1109	24	0.48408528222127245
1109	25	0.5968613656749753
1110	1	-0.08826353878546911
1110	2	-0.14835117648422624
1110	3	-0.07346772370932442
1110	4	-1.2159095477705222
1110	5	-0.3709864055187708
1110	6	-0.12660740575200435
1110	7	-0.1758044420424909
1110	8	-0.14844549886428382
1110	9	0.01778135516214263
1110	10	-0.23351398657639066
1110	11	-0.04264489895960821
1110	12	0.0044653520412651915
1110	13	-0.15170084305991338
1110	14	0.10106475352014144
1110	15	0.13391947007462335
1110	16	-0.0050888464583209725
1110	17	0.02506540998798812
1110	18	0.10100639175088588
1110	19	0.08403986882672143
1110	20	-0.1208078621936508
1110	21	0.15478396615394274
1110	22	-0.5419152072550091
1110	23	-0.09775364446872459
1110	24	0.051072554670128056
1110	25	-0.007747214838268235
1111	1	-0.12577693913559074
1111	2	-0.3700270775111356
1111	3	-0.32895762607175527
1111	4	-0.29197192030927965
1111	5	-0.25635159889890957
1111	6	-0.26178833303818294
1111	7	-0.10313321534646747
1111	8	-0.013049805023932493
1111	9	-0.28401546484904117
1111	10	-0.14864041008159745
1111	11	-0.4632967786181439
1111	12	-0.34828420128039994
1111	13	-0.43205204954261234
1111	14	-0.3253684973957718
1111	15	-0.30211186057563566
1111	16	-0.3927240681732087
1111	17	-0.45771103427197346
1111	18	-0.5003919370404617
1111	19	-0.4472539643050766
1111	20	-0.35649989778353586
1111	21	-0.4811011788586015
1111	22	-0.29956504753366175
1111	23	-0.1331785754366166
1111	24	-0.19655797466626268
1111	25	-0.14167960849272987
1112	1	-0.06216483786246485
1112	2	-0.2917461338596752
1112	3	-0.11479699497865781
1112	4	-0.21006523812553013
1112	5	-0.1367692417916757
1112	6	-0.030104336603500315
1112	7	0.041344097512912015
1112	8	0.04966437574811522
1112	9	0.052532975618312605
1112	10	0.22915341536002387
1112	11	0.11716598001920886
1112	12	0.08203028329052842
1112	13	-0.03476800271891989
1112	14	-0.15908621784953425
1112	15	-0.024042506836700284
1112	16	-0.09163286772087531
1112	17	-0.22647892768163302
1112	18	-0.23607958960821662
1112	19	-0.23906128199096344
1112	20	-0.786523620169369
1112	21	-1.219018252311578
1112	22	-1.312895797941792
1112	23	-0.3201939565364853
1112	24	-0.31153427791437593
1112	25	-1.365642294256439
1113	1	-0.14487927666412403
1113	2	-0.024998708057133
1113	3	-0.08869160430633727
1113	4	-0.41134185369651277
1113	5	-0.25881648588616457
1113	6	-0.17073705337189438
1113	7	-0.17850867237014256
1113	8	-0.2458861064771085
1113	9	-0.11874783321549387
1113	10	-0.0728127589865295
1113	11	-0.19887557987456006
1113	12	-0.14200643105508703
1113	13	-0.1314553250876429
1113	14	-0.5735250195082031
1113	15	-0.4075024145849184
1113	16	-0.40527960862382884
1113	17	-0.5341432802884398
1113	18	-0.8113719532106956
1113	19	-1.080812202179196
1113	20	-1.2283164729858385
1113	21	-0.8990816171720297
1113	22	-0.3793823096130424
1113	23	-1.835457433217286
1113	24	-1.071167134458193
1113	25	-0.3729525491136343
1114	1	-0.4588399251463464
1114	2	-0.5005816616888659
1114	3	-0.5382851002412963
1114	4	-0.7390294881383639
1114	5	-0.8917120456109942
1114	6	-0.83373934189013
1114	7	-0.5394822203980993
1114	8	-0.3653137707452956
1114	9	-1.3578869929925546
1114	10	-0.6651245747505001
1114	11	-0.644751092729436
1114	12	-0.5078944517273456
1114	13	-0.5974189424583898
1114	14	-1.061369070391656
1114	15	-1.5542005569678636
1114	16	-1.4747811514837181
1114	17	-1.8791136944896252
1114	18	-2.0810769199137837
1114	19	-1.8124061242046352
1114	20	-1.1356514144671266
1114	21	-1.1002506170277115
1114	22	-0.8186094364574288
1114	23	-0.796584923613068
1114	24	-1.3939719094205643
1114	25	-0.9125191738584988
1115	1	-0.581971919956537
1115	2	-0.3538161592626423
1115	3	-0.2839843712408978
1115	4	-0.3304445649921064
1115	5	-0.24159490150039886
1115	6	-0.1754707811825469
1115	7	-0.19456448796671566
1115	8	-0.05583929909390406
1115	9	-0.41498557939583236
1115	10	-0.07284808531347409
1115	11	-0.37685844660599344
1115	12	-0.29863453314817434
1115	13	-0.132320820097803
1115	14	-0.14929115922382863
1115	15	-1.32261325093571
1115	16	-0.630944185149481
1115	17	-0.5237750033300372
1115	18	-2.032426444070005
1115	19	-0.9713447465578895
1115	20	-0.6377309391167287
1115	21	-0.7653538065211478
1115	22	-0.4601471245914013
1115	23	-0.29159979831487703
1115	24	-0.27124661869382294
1115	25	-0.3953935983055459
1116	1	-1.2741518955962619
1116	2	-0.675854409732505
1116	3	-1.5399796605124294
1116	4	-0.6930859607873998
1116	5	-0.8738858427587957
1116	6	-0.4480793201845259
1116	7	-1.2404732525566686
1116	8	-3.0275345967006295
1116	9	-1.39733311930393
1116	10	-0.755359909188158
1116	11	-0.6970159010918024
1116	12	-0.7464609298979852
1116	13	-1.876131267052365
1116	14	-1.685776450296835
1116	15	-1.4254520563681083
1116	16	-0.9716633509848691
1116	17	-1.8393010481196015
1116	18	-2.43003733574745
1116	19	-1.3676697330468102
1116	20	-1.0213447561780598
1116	21	-0.9750007169877293
1116	22	-0.713989379718214
1116	23	-0.5414203227867347
1116	24	-0.5391424126478483
1116	25	-0.554019716498587
1201	1	0.3942343812850986
1201	2	0.5146613632349073
1201	3	0.5037194097970854
1201	4	0.5398187282436311
1201	5	0.44658724403065975
1201	6	0.40716082495477224
1201	7	0.3127582515413039
1201	8	0.5744325220452708
1201	9	0.43746280018685724
1201	10	0.617355774757975
1201	11	0.43591127918259076
1201	12	0.4715323007096117
1201	13	0.5608142176339328
1201	14	0.874942042695487
1201	15	0.6401901315423046
1201	16	0.6439357418158878
1201	17	0.8895229744156836
1201	18	0.6788442675164532
1201	19	0.5681500098609717
1201	20	0.45592178707511977
1201	21	0.7052253121439378
1201	22	0.6765942601828243
1201	23	0.6969546141040937
1201	24	0.8770328806426875
1201	25	0.8315026072400613
1202	1	0.33958366586922395
1202	2	0.41082919285551256
1202	3	0.37402026010044054
1202	4	0.5651579958672244
1202	5	0.31857595218423995
1202	6	0.3184014482829056
1202	7	0.3504793930194836
1202	8	0.31618389653161927
1202	9	0.3071312160273081
1202	10	0.6118186721638068
1202	11	0.45751194394451744
1202	12	0.31779699889175816
1202	13	0.5514410744710787
1202	14	0.8258712296142505
1202	15	0.6426470187718181
1202	16	0.5734186162282455
1202	17	0.4676659665273206
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2210	22	0.6029160848096566
2210	23	0.6383118438632494
2210	24	0.5834502807297781
2210	25	0.711087150361621
2211	1	0.007924125434268348
2211	2	-0.10581214749126033
2211	3	0.09140518940207087
2211	4	0.07911659194830634
2211	5	0.049038695834717316
2211	6	0.1531159376038147
2211	7	-0.009458675412844286
2211	8	0.17258058667896137
2211	9	0.053278641868359955
2211	10	0.2624782843737634
2211	11	0.185492093031435
2211	12	0.1105682314735077
2211	13	-0.039852823628940826
2211	14	-0.21838482255591884
2211	15	-0.08733216647944886
2211	16	-0.15434907887157578
2211	17	0.03346948760353996
2211	18	0.005230100791891346
2211	19	0.15220683797372914
2211	20	0.07423832249091333
2211	21	0.11109460894723355
2211	22	0.08579307528871993
2211	23	0.27945252071263127
2211	24	0.16463866242536085
2211	25	0.031387059344361035
2212	1	0.12501435327913413
2212	2	0.171843159752477
2212	3	0.1919535569839823
2212	4	0.1679255290448154
2212	5	0.20035337650950424
2212	6	0.10614437785196884
2212	7	0.13437720106163198
2212	8	0.14695083325809577
2212	9	0.04917803743990845
2212	10	0.28857994260468445
2212	11	0.1772614344758523
2212	12	0.077735365609783
2212	13	0.19869789638071467
2212	14	0.31976655244341856
2212	15	0.25708312394603183
2212	16	0.16520747253323265
2212	17	0.3909742820499673
2212	18	0.33101295716429036
2212	19	0.3771776987387483
2212	20	0.25785541963766434
2212	21	0.36647565434057583
2212	22	-0.3886241843636
2212	23	0.2527221291558277
2212	24	0.25390531170606323
2212	25	0.2694410374486864
2213	1	-0.05914986959476565
2213	2	-0.7137873387812483
2213	3	-0.17344233744416826
2213	4	-0.22562285805195614
2213	5	-0.09348826571923041
2213	6	-0.04517527024187018
2213	7	-0.11547617137238575
2213	8	0.054868721821144106
2213	9	-0.27926771036067777
2213	10	-1.771244104316139
2213	11	-0.5365215567264912
2213	12	-0.2841414672347895
2213	13	0.015120183770740425
2213	14	-0.1875648258557021
2213	15	-0.263737990423472
2213	16	-0.3344992723348028
2213	17	-0.14509609918170696
2213	18	-0.010922526706679037
2213	19	-0.19232376753691227
2213	20	-0.15504780616878833
2213	21	-0.06226334859197635
2213	22	-0.12938664770670982
2213	23	-0.8634069031022378
2213	24	-1.3566518730446688
2213	25	-1.556951924428148
2214	1	-0.06332417311382699
2214	2	-0.26406979322196733
2214	3	0.02615214569366131
2214	4	0.03476642504397631
2214	5	0.07969656356438293
2214	6	-0.1320298471166126
2214	7	-0.07326379705315134
2214	8	-0.07269889705191446
2214	9	-0.32057119014702096
2214	10	0.04447908378608645
2214	11	-0.19869000823466365
2214	12	-0.2013332220525541
2214	13	-0.1553412348509582
2214	14	0.011420563754683144
2214	15	-0.04834798728145273
2214	16	-0.062184062343185076
2214	17	-0.11627887306105578
2214	18	-0.0034419024921845676
2214	19	-0.6790628097447069
2214	20	-0.15584051144737998
2214	21	-0.20830538619147632
2214	22	-0.27645278721236366
2214	23	-1.7531171172832174
2214	24	-1.2121883728352003
2214	25	-1.6020199360536225
2215	1	-0.27858873177147103
2215	2	-0.18724435862765532
2215	3	-1.25580948360696
2215	4	-0.7813729246256713
2215	5	-0.32877613706408937
2215	6	-0.41608859540441756
2215	7	-0.31601405388177284
2215	8	-0.2729468499767407
2215	9	-0.42621052974496365
2215	10	-1.3217509742269655
2215	11	-0.41383968644350944
2215	12	-0.6006730843722679
2215	13	-0.5489912101752665
2215	14	-0.7931844741149447
2215	15	-1.115236105684481
2215	16	-0.8230844858586153
2215	17	-0.5923116640327706
2215	18	-0.5423514383877617
2215	19	-0.8370656021264651
2215	20	-1.330681000731083
2215	21	-0.5933723756564646
2215	22	-0.442622163169176
2215	23	-0.5507899856056753
2215	24	-1.4937417831164055
2215	25	-0.7200355195078103
2216	1	-0.6721420923950596
2216	2	-0.47783121496836145
2216	3	-1.419724119993731
2216	4	-0.9505696187788573
2216	5	-0.69742721913466
2216	6	-0.548062821201853
2216	7	-0.3430404809012515
2216	8	-0.5697279059957694
2216	9	-0.30236611058953144
2216	10	-0.4101013293831741
2216	11	-0.33514645279907995
2216	12	-0.40865160737159556
2216	13	-1.0916223422382956
2216	14	-0.7643630419009284
2216	15	-1.2530831097246136
2216	16	-0.888646415146539
2216	17	-2.053956820559222
2216	18	-1.0865468857861273
2216	19	-0.8545340045332281
2216	20	-0.6879417307856599
2216	21	-0.45889016178026054
2216	22	-0.6336784106334363
2216	23	-0.6042801108249023
2216	24	-0.8239088418271336
2216	25	-0.5808359071439924
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1349.0
3	151.0
4	164.0
5	147.0
6	90.0
7	66.0
8	134.0
9	277.0
10	538.0
11	959.0
12	1601.0
13	2404.0
14	3255.0
15	4237.0
16	4834.0
17	5003.0
18	5340.0
19	5479.0
20	5434.0
21	6066.0
22	6233.0
23	6704.0
24	6928.0
25	7404.0
26	7966.0
27	8731.0
28	9725.0
29	11460.0
30	14124.0
31	17955.0
32	24209.0
33	34585.0
34	51016.0
35	81015.0
36	151755.0
37	541273.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.589479022411204	20.562620686964834	13.630263902163101	23.217636388460864
2	15.200543860964826	20.66944375996486	39.99501330920502	24.13499906986529
3	19.09825150335253	25.489630691089182	29.493083068446495	25.91903473711179
4	12.602258156293725	18.393119521710492	36.70000896403085	32.304613357964925
5	12.920385204273074	38.79858058014547	34.70785622185209	13.573177993729368
6	31.406960773808944	37.46018449070241	17.340372682907823	13.792482052580823
7	26.698245162899063	33.00917159532771	22.08020665310798	18.21237658866525
8	24.490666812160278	37.32515191579038	20.458480247780816	17.725701024268528
9	23.99660558208324	18.228238556261697	21.71911094310063	36.056044918554434
10	15.145775052057298	30.993897335309416	33.00788386715816	20.85244374547513
11	31.61424624868007	26.156859918252202	22.19240345538355	20.036490377684178
12	21.28483695991022	28.489833374898684	30.460323274518363	19.765006390672735
13	26.994176456202606	23.620184517772184	25.91286996358945	23.472769062435756
14	20.310775330868562	24.624220017243648	27.469032066363685	27.595972585524102
15	24.220893771923247	30.060890732744134	23.4098615517542	22.308353943578417
16	21.026719089127273	31.26215256806412	26.611542552984226	21.099585789824378
17	20.73045873829677	30.632332326384056	27.99189115206233	20.645317783256846
18	20.855600907913065	29.589393393276374	29.097249958598386	20.45775574021217
19	21.150228590299744	29.550934283089582	29.417472228689668	19.88136489792101
20	22.19311789201958	28.663857974332124	28.61486009302779	20.52816404062051
21	21.829805347939462	29.183587391790965	28.319376271794006	20.667230988475563
22	21.202672215273473	28.877692964101602	28.224250996528223	21.695383824096695
23	20.69313865598201	29.444170054329245	28.42811482300863	21.43457646668011
24	21.1676236260658	29.603708708898896	28.21099420173217	21.017673463303133
25	21.61346673018898	28.33143838092148	27.288678706677928	22.76641618221161
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2704.0
1	2704.0
2	2233.5
3	1763.0
4	1763.0
5	1763.0
6	2312.5
7	2862.0
8	2862.0
9	2862.0
10	3852.0
11	4842.0
12	4842.0
13	4842.0
14	7162.0
15	9482.0
16	9482.0
17	9482.0
18	14658.0
19	19834.0
20	19834.0
21	19834.0
22	28135.0
23	36436.0
24	36436.0
25	36436.0
26	47437.0
27	58438.0
28	58438.0
29	58438.0
30	69696.5
31	80955.0
32	80955.0
33	80955.0
34	90435.0
35	99915.0
36	99915.0
37	99915.0
38	105384.5
39	110854.0
40	110854.0
41	110854.0
42	112639.0
43	114424.0
44	114424.0
45	114424.0
46	127104.0
47	139784.0
48	139784.0
49	139784.0
50	128679.5
51	117575.0
52	117575.0
53	117575.0
54	101975.5
55	86376.0
56	86376.0
57	86376.0
58	74491.5
59	62607.0
60	62607.0
61	62607.0
62	51935.0
63	41263.0
64	41263.0
65	41263.0
66	31741.5
67	22220.0
68	22220.0
69	22220.0
70	16148.0
71	10076.0
72	10076.0
73	10076.0
74	7083.0
75	4090.0
76	4090.0
77	4090.0
78	2770.5
79	1451.0
80	1451.0
81	1451.0
82	948.0
83	445.0
84	445.0
85	445.0
86	302.0
87	159.0
88	159.0
89	159.0
90	104.0
91	49.0
92	49.0
93	49.0
94	27.5
95	6.0
96	6.0
97	6.0
98	3.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3141129153781167
2	0.1825763092169926
3	0.2014366947271612
4	0.22233866835956448
5	0.24868487698459377
6	0.19453418250436755
7	0.19083988018794276
8	0.21582503006481557
9	0.21582503006481557
10	0.22729681094213458
11	0.1982284848207923
12	0.20561708945364185
13	0.2197137693452627
14	0.20863086239598835
15	0.19618689669855757
16	0.20211722410123945
17	0.20202000561922828
18	0.2023116610652618
19	0.20435324918749656
20	0.1979368293747588
21	0.19540914884246816
22	0.19900623267688172
23	0.20338106436738473
24	0.22068595416537445
25	0.21388066042459197
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1028611.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.26355180126883
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.1327072409118	74.87031027462972
2	6.28153312356356	10.209193847561867
3	0.8109755516649063	1.97708261256851
4	0.24238959818032169	0.7878975867126122
5	0.1264154914474961	0.5136485918863227
6	0.08907443424060782	0.43431029406482075
7	0.05736022728663967	0.3262907061008277
8	0.040793786357101694	0.26520383766401817
9	0.030446516126312648	0.22267728363588962
>10	0.16624371868170842	2.473021494655647
>50	0.009595071724603959	0.548381559077615
>100	0.007670918498557751	1.2289074850590216
>500	0.002876592789869696	1.7576965450849822
>1k	0.0016780124607573227	2.8595928664052974
>5k	2.3971606582247465E-4	1.5257850148928431
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	8085	0.7860114270603756	No Hit
CCCATGTACTCTGCGTTGATACCAC	7580	0.7369160936447307	No Hit
GAGTACATGGGAAGCAGTGGTATCA	3679	0.3576667953191245	No Hit
CATGTACTCTGCGTTGATACCACTG	2793	0.27153122025722065	No Hit
GCGTTGATACCACTGCTTCCCATGT	2464	0.239546339675543	No Hit
GCTTCCCATGTACTCTGCGTTGATA	2293	0.22292197925163157	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	2253	0.21903323997118446	No Hit
GTATCAACGCAGAGTACATGGGAAG	2207	0.21456118979867023	No Hit
GGTATCAACGCAGAGTACATGGGAA	2158	0.20979748418012253	No Hit
TATCAACGCAGAGTACATGGGAAGC	1990	0.19346477920224459	No Hit
ACTCTGCGTTGATACCACTGCTTCC	1871	0.18189577984291438	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1776	0.17266002405185246	No Hit
GTGGTATCAACGCAGAGTACATGGG	1772	0.17227115012380773	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1717	0.16692413361319292	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	1234	0.11996760680179387	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1152	0.11199569127687728	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	9.721848201117818E-5	0.0
9	0.0	0.0	0.0	1.9443696402235636E-4	0.0
10	0.0	0.0	0.0	1.9443696402235636E-4	0.0
11	0.0	0.0	0.0	2.9165544603353453E-4	0.0
12	0.0	0.0	0.0	2.9165544603353453E-4	0.0
13	0.0	0.0	0.0	2.9165544603353453E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCTTAG	90	2.242814E-9	14.797189	1
ATAGGGG	40	0.005284495	14.2471485	8
CCTATAC	100	9.95351E-9	13.309669	3
TTAGACT	75	1.4620111E-5	12.679588	4
GTCTCGC	70	1.0769229E-4	12.230329	1
GTAGGAC	55	0.00305057	12.099699	3
GTACAAG	145	9.458745E-11	11.808593	1
CTAGCCT	100	1.9037816E-6	11.41163	4
CTTAGAC	60	0.0058416566	11.091392	3
GTCTAAC	95	1.3392024E-5	11.014448	1
GTATTAA	175	2.0008883E-11	10.871404	1
TAAGGGT	80	3.73297E-4	10.698403	4
TAGGACA	145	1.4171746E-8	10.493453	4
TAGACAG	185	5.820766E-11	10.280747	5
CCAGGAC	150	2.4114343E-8	10.1407	3
TCTCGCT	75	0.0026314142	10.140205	2
AGGACTG	180	3.8562575E-10	10.038008	5
TCTAGCC	95	1.63058E-4	10.00727	3
GTACTAT	115	1.0091766E-5	9.9260645	1
CCTAGAC	115	1.0162299E-5	9.920251	3
>>END_MODULE