FastQCFastQC Report
Wed 25 May 2016
SRR1294992_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294992_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1028611
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC86900.8448286086771385No Hit
GTACATGGGAAGCAGTGGTATCAAC78960.7676371339602629No Hit
GGTATCAACGCAGAGTACTTTTTTT43240.42037271621633443No Hit
GAGTACATGGGAAGCAGTGGTATCA40440.39315154125320456No Hit
GTATCAACGCAGAGTACTTTTTTTT39680.385762936620355No Hit
CATGTACTCTGCGTTGATACCACTG30760.29904405066638406No Hit
TATCAACGCAGAGTACTTTTTTTTT28340.275517178019679No Hit
ACGCAGAGTACATGGGAAGCAGTGG27930.27153122025722065No Hit
GCGTTGATACCACTGCTTCCCATGT27390.266281422228617No Hit
GCTTCCCATGTACTCTGCGTTGATA25740.25024037269677263No Hit
GGTATCAACGCAGAGTACATGGGAA22650.22019986175531858No Hit
GTATCAACGCAGAGTACATGGGAAG22590.2196165508632515No Hit
TATCAACGCAGAGTACATGGGAAGC20280.19715908151866934No Hit
ACTCTGCGTTGATACCACTGCTTCC20090.19531193036045696No Hit
GTGGTATCAACGCAGAGTACATGGG18050.1754793600301766No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17250.16770188146928236No Hit
ACATGGGAAGCAGTGGTATCAACGC11740.11413449788112318No Hit
CAGTGGTATCAACGCAGAGTACATG11570.11248178368693316No Hit
CATGGGAAGCAGTGGTATCAACGCA11310.10995410315464253No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACCCG400.00528126214.2484689
CCGTGTT550.003069702812.089619
TAGGGTA658.0233347E-411.691624
CTAGGAC1657.2759576E-1211.5144743
ACTGATC1104.9651135E-711.2266128
ACCGTGT855.321759E-511.1758148
TAAAGGT803.770389E-410.6868714
GTCTAGG909.363663E-510.5713791
GTCCAAT909.363663E-510.5713791
GACAGTA909.5126554E-510.55493457
TAGGACA1355.680522E-810.55493454
TCTAGGA1451.4411853E-810.4821422
TAGACTG2001.8189894E-1210.4493865
TATACTG2001.8189894E-1210.4493865
CTGATCA1106.0453767E-610.3625239
TGTTAGA1201.5224887E-610.2910622
GTCTCGC750.002613583310.1485231
TCAGGCC856.592471E-410.0582323
CCTACAC951.6435712E-49.9994123
AACAGGC951.6435712E-49.9994127
Produced by FastQC (version 0.11.5)