FastQCFastQC Report
Wed 25 May 2016
SRR1294991_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294991_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3104202
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC164110.5286704924486229No Hit
CCCATGTACTCTGCGTTGATACCAC147720.47587109344043976No Hit
GAGTACATGGGAAGCAGTGGTATCA78530.25297967078173395No Hit
GTATCAACGCAGAGTACTTTTTTTT61430.19789304948582598No Hit
CATGTACTCTGCGTTGATACCACTG56780.18291335422114927No Hit
GGTATCAACGCAGAGTACTTTTTTT54250.17476311142122838No Hit
GCTTCCCATGTACTCTGCGTTGATA49970.16097534889804208No Hit
GCGTTGATACCACTGCTTCCCATGT47660.1535338228633317No Hit
GTATCAACGCAGAGTACATGGGAAG46390.14944259426416193No Hit
GGTATCAACGCAGAGTACATGGGAA43940.14155006665159034No Hit
ACGCAGAGTACATGGGAAGCAGTGG43140.13897291477809756No Hit
TATCAACGCAGAGTACTTTTTTTTT41950.13513940136627706No Hit
TATCAACGCAGAGTACATGGGAAGC38070.12264021477983714No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN36790.1185167717822487No Hit
ATTCCATTCCATTCCATTCCATTCC34540.1112685321380503No Hit
ACTCTGCGTTGATACCACTGCTTCC33060.10650080117208867No Hit
GTGGTATCAACGCAGAGTACATGGG31980.10302164614287344No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGC456.734992E-414.78644853
TATACCG551.950337E-413.8271855
GACGGTG550.00307359712.0888237
AACTGCG550.00307359712.0888237
GCGTAGT600.005826988711.0962921
GTCTAGG3400.010.0705841
GTATAAG4700.09.5111071
GTCTTAC3400.09.5111071
GTTAGAC1701.6507329E-89.5055743
TAGGACT3700.09.2495634
CCTACAC3450.09.0922893
ACTGCGT1151.1133257E-49.0853898
GTTCTAG3600.08.9827121
CCAGGAC3400.08.9464243
GTCTTAG3000.08.8770331
CTATACT4950.08.8343194
CTTACTC3900.08.7743763
GTCCTAC2401.0004442E-108.7185151
GGAACGA1201.7701488E-48.70584815
GTCTAGA2751.8189894E-128.64646151
Produced by FastQC (version 0.11.5)