##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294991_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3104202
Sequences flagged as poor quality	0
Sequence length	25
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.968787147228177	33.0	33.0	33.0	27.0	33.0
2	30.87929168269333	33.0	33.0	33.0	27.0	33.0
3	30.974097368663507	33.0	33.0	33.0	27.0	33.0
4	34.49672508425676	37.0	37.0	37.0	27.0	37.0
5	34.74938840964602	37.0	37.0	37.0	27.0	37.0
6	34.60201945620807	37.0	37.0	37.0	27.0	37.0
7	34.52576475371126	37.0	37.0	37.0	27.0	37.0
8	34.46009312538295	37.0	37.0	37.0	27.0	37.0
9	34.42913702136652	37.0	37.0	37.0	27.0	37.0
10	34.359988493016886	37.0	37.0	37.0	27.0	37.0
11	34.51511499573804	37.0	37.0	37.0	27.0	37.0
12	34.428740462121986	37.0	37.0	37.0	27.0	37.0
13	34.334262718727715	37.0	37.0	37.0	27.0	37.0
14	35.641399303266994	37.0	37.0	40.0	27.0	40.0
15	35.7943590655505	37.0	37.0	40.0	27.0	40.0
16	35.88101934088052	37.0	37.0	40.0	27.0	40.0
17	35.80263268949636	37.0	37.0	40.0	27.0	40.0
18	35.74534614693245	37.0	37.0	40.0	27.0	40.0
19	35.8461253488014	40.0	37.0	40.0	27.0	40.0
20	35.860944294217965	40.0	37.0	40.0	27.0	40.0
21	35.85377530199388	40.0	37.0	40.0	27.0	40.0
22	35.7423341006803	37.0	37.0	40.0	27.0	40.0
23	35.642213683259016	37.0	37.0	40.0	27.0	40.0
24	35.71578170492771	37.0	37.0	40.0	27.0	40.0
25	34.51843082376727	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.44869898235940653
1101	2	0.37412307066929884
1101	3	0.4242478745026901
1101	4	0.5463046703694303
1101	5	0.43352411953031833
1101	6	0.2889690919287844
1101	7	0.2590813677319659
1101	8	0.38650817370957213
1101	9	0.41950516152869
1101	10	0.5428724810461887
1101	11	0.43715718825305316
1101	12	0.44048493262998534
1101	13	0.5126455559110283
1101	14	0.831018142971871
1101	15	0.803646367650579
1101	16	0.7095964761696578
1101	17	0.7530228961314407
1101	18	0.6787885473452633
1101	19	0.5841159107161005
1101	20	0.6469923915730931
1101	21	0.6545682200763423
1101	22	0.5877079466189485
1101	23	0.6660219963831153
1101	24	0.6215346989278459
1101	25	0.7934289927365157
1102	1	0.453673187043119
1102	2	0.4119436258109168
1102	3	0.4004534636450785
1102	4	0.5528506445080907
1102	5	0.34568517284692746
1102	6	0.2419692711284256
1102	7	0.3462346400116658
1102	8	0.36806900255505326
1102	9	0.3606338363002095
1102	10	0.5247157851102457
1102	11	0.3411976685080873
1102	12	0.4521173470150046
1102	13	0.3179333599609393
1102	14	0.6991182495484978
1102	15	0.6747626870786476
1102	16	0.7154572474826253
1102	17	0.6394518246119247
1102	18	0.589024147711207
1102	19	0.7139866040300689
1102	20	0.6914414470023686
1102	21	0.723077184774013
1102	22	0.7460397866219637
1102	23	0.8098608298877892
1102	24	0.741929504024661
1102	25	0.8359931509226399
1103	1	0.34468137060639137
1103	2	0.35054856588418204
1103	3	0.3374405877231652
1103	4	0.44106361921650006
1103	5	0.2659212501249115
1103	6	0.1756436798126586
1103	7	0.23261969032168395
1103	8	0.27268278102633303
1103	9	0.2982625113263069
1103	10	0.4850661029857335
1103	11	0.3570149209711957
1103	12	0.4092831100097314
1103	13	0.38328698942925143
1103	14	0.6853105913497757
1103	15	0.5689973349459478
1103	16	0.5218415119909565
1103	17	0.45912362144102303
1103	18	0.4888510842446223
1103	19	0.40880873220555003
1103	20	0.5156302938571073
1103	21	0.4672618781055462
1103	22	0.548281753670679
1103	23	0.5471800487260694
1103	24	0.5351528999601953
1103	25	0.5016814944901
1104	1	0.2989293006270586
1104	2	0.2624516200833966
1104	3	0.3525742191607186
1104	4	0.26130206032732417
1104	5	0.11561710002239778
1104	6	0.12822945185058643
1104	7	0.05802905989428808
1104	8	0.2539578684481256
1104	9	0.28126288014345135
1104	10	0.4225087796683553
1104	11	0.16129324578792392
1104	12	0.2926782712096525
1104	13	0.2822093615979071
1104	14	0.5694326825043987
1104	15	0.45059674072216893
1104	16	0.3936290861793452
1104	17	0.4126760453892615
1104	18	0.47067356283181994
1104	19	0.4633667938922841
1104	20	0.4244874366617424
1104	21	0.4052289752793996
1104	22	0.3740417476137736
1104	23	0.4689222696153834
1104	24	0.4691672494647037
1104	25	0.35633207286411306
1105	1	0.2588227262267999
1105	2	0.14710728988411503
1105	3	0.20539993996784744
1105	4	0.3186316234276063
1105	5	0.23957163572902118
1105	6	0.1700702561215479
1105	7	0.25429623090350617
1105	8	0.4217779342278902
1105	9	0.3258897479929388
1105	10	0.3854162559086518
1105	11	0.2752011608331273
1105	12	0.4044943025436254
1105	13	0.39157525698890083
1105	14	0.6859524372173809
1105	15	0.6049313234503799
1105	16	0.518592279364384
1105	17	0.34934618318541055
1105	18	0.362692851098835
1105	19	0.48146973992425046
1105	20	0.48163191274881
1105	21	0.472227113868378
1105	22	0.3799481913530087
1105	23	0.5385840317293642
1105	24	0.48757960979730797
1105	25	0.3903752409001555
1106	1	0.005749219071731204
1106	2	-0.2986035828620359
1106	3	-0.22458445657078485
1106	4	-0.13670692636279824
1106	5	-0.4992309236859711
1106	6	-0.5002714543850928
1106	7	-0.8846519745512964
1106	8	-0.3639153024671842
1106	9	-0.027634646811321772
1106	10	-0.23297782752876373
1106	11	-0.478672923765771
1106	12	-0.9368175339953524
1106	13	-0.3702273499054982
1106	14	-0.4988711806902799
1106	15	-0.27633061261878566
1106	16	-0.3252117848732965
1106	17	-0.3581448320225178
1106	18	-0.5631800147310173
1106	19	-0.7001931617427459
1106	20	-0.13316997057504665
1106	21	-0.06252570674223534
1106	22	0.04592822135528962
1106	23	-0.8894506188241209
1106	24	-0.13636436567349364
1106	25	-0.0011151731634839734
1107	1	-1.158746133982259
1107	2	-1.2019864605886283
1107	3	-1.03113812566939
1107	4	-1.0542667241641581
1107	5	-1.1710514490513688
1107	6	-1.1163682624924576
1107	7	-1.129449486469774
1107	8	-1.0479162510902782
1107	9	-1.1295974843148002
1107	10	-1.0274981753074357
1107	11	-0.9368527438635113
1107	12	-1.132480384383669
1107	13	-1.0371105534878566
1107	14	-1.3646227123991252
1107	15	-1.0627024654029356
1107	16	-1.1401408808989757
1107	17	-1.1670743071239613
1107	18	-1.349395101946449
1107	19	-1.3774532842794542
1107	20	-1.3674828630725244
1107	21	-1.2812615264142195
1107	22	-1.3058461053183876
1107	23	-0.8949471240443501
1107	24	-1.0169256685737054
1107	25	-1.0187880401153606
1108	1	-0.10767307480515598
1108	2	-0.2585014399028651
1108	3	-0.10802338025967728
1108	4	-0.15816826071189638
1108	5	-0.4472848149069577
1108	6	-0.14854871585313134
1108	7	-0.011753769818028559
1108	8	0.15859298475630368
1108	9	-0.2722303270095736
1108	10	-0.6822721724330165
1108	11	-0.22342930098771063
1108	12	-0.01379043945290448
1108	13	0.059548604393981464
1108	14	-0.4274886677007217
1108	15	-0.22611424604907882
1108	16	-0.855564329136719
1108	17	-0.9393525169851173
1108	18	-0.7198261631144831
1108	19	-0.2695764431990355
1108	20	-1.0533276797321633
1108	21	-0.3684362290892267
1108	22	-0.4033787652327163
1108	23	-0.24179363033525192
1108	24	-0.6511196558951795
1108	25	-1.212114563778961
1109	1	0.1431793850135321
1109	2	0.02483926306989659
1109	3	0.07022855599953104
1109	4	0.10910932901254711
1109	5	0.06446016430624013
1109	6	0.0735203302618217
1109	7	0.18304065406972114
1109	8	0.21206645008515324
1109	9	0.22135243976993735
1109	10	0.23911865407224298
1109	11	0.17067303150930968
1109	12	0.20838098777168312
1109	13	0.19631675439188
1109	14	0.5218769172370941
1109	15	0.38416248493436456
1109	16	0.2909662811373508
1109	17	0.3342197923537995
1109	18	0.33727565273029825
1109	19	0.25179502010036003
1109	20	0.2640199195208339
1109	21	0.3149314072353917
1109	22	0.3867894728734953
1109	23	0.29086316109641785
1109	24	0.3514005480060902
1109	25	0.2889663712298187
1110	1	-0.006686820169569785
1110	2	0.04333293064890498
1110	3	0.09124060485087071
1110	4	-0.8893289026431148
1110	5	-0.21285206265518042
1110	6	0.02192048095231769
1110	7	0.09996690119808704
1110	8	0.17706826821978439
1110	9	0.09011974934550437
1110	10	-0.17249800924277991
1110	11	-0.07828094473939018
1110	12	0.07141298621334613
1110	13	0.04405370035103573
1110	14	0.22338550403181756
1110	15	0.14331131653725748
1110	16	0.10372129806900432
1110	17	0.10491820590701906
1110	18	0.28110382352353014
1110	19	0.011064332939270116
1110	20	0.011529131685946936
1110	21	0.07366530631303192
1110	22	-0.4708287133566955
1110	23	0.025776828204826074
1110	24	0.11338651056747295
1110	25	0.1399700163127946
1111	1	-0.21086944912971717
1111	2	-0.33510594090989443
1111	3	-0.28261637220255764
1111	4	-0.2703980509438324
1111	5	-0.3107679567599888
1111	6	-0.17656246458783897
1111	7	-0.11527303549388535
1111	8	-0.026788958284953424
1111	9	-0.015999705632879113
1111	10	-0.14724624473341663
1111	11	-0.1220199201671619
1111	12	-0.05970663015126121
1111	13	-0.2104867716889416
1111	14	-0.35709944206000444
1111	15	-0.2364974252407137
1111	16	-0.25303523402864414
1111	17	-0.21919256194953363
1111	18	-0.3121284429790947
1111	19	-0.304320859328314
1111	20	-0.2688798791965823
1111	21	-0.347293217904344
1111	22	-0.23731041148091947
1111	23	-0.11967508073126254
1111	24	-0.049841593365890446
1111	25	-0.12555177246187554
1112	1	-0.12993897117389963
1112	2	-0.4692824190797751
1112	3	-0.1284367055820681
1112	4	-0.17702337489137676
1112	5	0.016522560910033235
1112	6	-0.037665504576217756
1112	7	-0.014190553297943609
1112	8	0.12342844776262751
1112	9	-0.09269238881370256
1112	10	0.2346340347317053
1112	11	-0.0022911088196195806
1112	12	-0.0019629700092451685
1112	13	-0.05364810761117411
1112	14	-0.27205218260079533
1112	15	-0.33152562792203355
1112	16	-0.17454703852776277
1112	17	-0.16115577005796666
1112	18	-0.11156798921628308
1112	19	-0.0025705702946225983
1112	20	-0.6970913673255552
1112	21	-1.104505399971302
1112	22	-1.5499375115754503
1112	23	-0.4300737206256571
1112	24	-0.23085310039414253
1112	25	-1.2011196589787474
1113	1	-0.10674112233369826
1113	2	-0.10324470641099737
1113	3	-0.20633187419130294
1113	4	-0.46168094496853485
1113	5	-0.1988520095911781
1113	6	-0.0734335296545865
1113	7	-0.15578398885231337
1113	8	0.06440324653723906
1113	9	-0.11192085640958283
1113	10	0.04119824257146831
1113	11	-0.07767986714704733
1113	12	-0.1833693481901122
1113	13	-0.10009167929734986
1113	14	-0.49680383619590174
1113	15	-0.36607647225621776
1113	16	-0.380353624374024
1113	17	-0.3062314847003549
1113	18	-0.7664771752392951
1113	19	-0.9985420598974812
1113	20	-1.118432711364484
1113	21	-0.8851879215998153
1113	22	-0.420119139529433
1113	23	-2.0079402757976723
1113	24	-0.9674176252803051
1113	25	-0.3925414931714073
1114	1	-0.28017716037936324
1114	2	-0.3267570759001046
1114	3	-0.3138413236444748
1114	4	-0.450610019851581
1114	5	-0.604964527792994
1114	6	-0.3165391466574121
1114	7	-0.23873384478688564
1114	8	-0.029911861744196244
1114	9	-1.0854600638947858
1114	10	-0.2928027875738337
1114	11	-0.3272196710321609
1114	12	-0.33714328164117546
1114	13	-0.21964182336277815
1114	14	-0.6149154559251215
1114	15	-1.1567169825266888
1114	16	-1.0324467169786615
1114	17	-1.2841656948190163
1114	18	-1.5488867857978974
1114	19	-1.3508131817489542
1114	20	-0.7475520920380276
1114	21	-0.44394256887186145
1114	22	-0.3209367108650554
1114	23	-0.11497678786508914
1114	24	-0.7441215863051838
1114	25	-0.40983468956513036
1115	1	-0.6541976015629629
1115	2	-0.49841998184382064
1115	3	-0.4879406607879311
1115	4	-0.643122802581523
1115	5	-0.3701892810858354
1115	6	-0.4396746296143874
1115	7	-0.4123662371472534
1115	8	-0.11571844128511799
1115	9	-0.514666667854776
1115	10	-0.23210497541052888
1115	11	-0.5305362605271213
1115	12	-0.5892844937589956
1115	13	-0.4686902253118319
1115	14	-0.3128279284769704
1115	15	-1.4668029199393118
1115	16	-0.8807731952643323
1115	17	-0.6754773063656927
1115	18	-2.223127557813271
1115	19	-1.0299135348002224
1115	20	-0.8639917871911251
1115	21	-0.8367905079770424
1115	22	-0.6229061040791635
1115	23	-0.26060507558509727
1115	24	-0.2862026548165133
1115	25	-0.4227448739943327
1116	1	-1.2018355974593504
1116	2	-0.5295546578170622
1116	3	-1.4776778172894076
1116	4	-0.709283457764549
1116	5	-0.74907604401605
1116	6	-0.2121010570739088
1116	7	-0.964393386034395
1116	8	-2.601289082135537
1116	9	-1.2011178546328054
1116	10	-0.5026338007774953
1116	11	-0.2998458439361542
1116	12	-0.5746996721594897
1116	13	-1.461655554371788
1116	14	-1.3964039197857119
1116	15	-1.1750777211978516
1116	16	-0.6391760872712311
1116	17	-1.4532236545328772
1116	18	-2.117087606501883
1116	19	-1.0504901047102493
1116	20	-0.6650628941534222
1116	21	-0.3805542009604892
1116	22	-0.1526206430667898
1116	23	-0.09602423450117215
1116	24	-0.30559778094179535
1116	25	-0.1574773733122541
1201	1	0.41293065711794696
1201	2	0.37211264645732456
1201	3	0.4116602466200483
1201	4	0.45433663996752216
1201	5	0.35413219541419494
1201	6	0.13245613154891345
1201	7	0.08789234838040016
1201	8	0.2607220844323024
1201	9	0.1797967334805861
1201	10	0.4605251728972988
1201	11	0.3134593411861957
1201	12	0.44476775572342575
1201	13	0.3756548081310598
1201	14	0.8318415593897655
1201	15	0.7272139828972399
1201	16	0.6353820932503353
1201	17	0.6332821509485527
1201	18	0.56608730896248
1201	19	0.5394164591252206
1201	20	0.5411257266692928
1201	21	0.5170482343555634
1201	22	0.6157400862078148
1201	23	0.6156931114564799
1201	24	0.6876687593180151
1201	25	0.7534152423717444
1202	1	0.4200870803126655
1202	2	0.36815758522975983
1202	3	0.3911046028030647
1202	4	0.5554091363041422
1202	5	0.48984605943923043
1202	6	0.15068396154492802
1202	7	0.15134107806380825
1202	8	0.3260960758321758
1202	9	0.3393852923037386
1202	10	0.5419424039704666
1202	11	0.3441293286157432
1202	12	0.4008394193094702
1202	13	0.39910597606973397
1202	14	0.6889430018481448
1202	15	0.5860984235168445
1202	16	0.5171186493683635
1202	17	0.5394700396354821
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2210	20	0.4867860686586951
2210	21	0.49382660415418655
2210	22	0.5422830450165179
2210	23	0.6642440467239936
2210	24	0.5638458034892935
2210	25	0.5990995294307879
2211	1	0.04692956880226973
2211	2	-0.1816225458336831
2211	3	0.012393063368545398
2211	4	-0.04517028818699487
2211	5	-0.027562632808056264
2211	6	0.048060492873226224
2211	7	0.052234412482370374
2211	8	0.04756029955954233
2211	9	-0.03665900265280442
2211	10	0.19937252216514167
2211	11	0.0533818622230271
2211	12	0.008863590593861659
2211	13	-0.24249885818714745
2211	14	-0.21308388397619638
2211	15	-0.18235197893459798
2211	16	-0.22047662900396858
2211	17	-0.13689300478485933
2211	18	0.048822784285668774
2211	19	-0.06763077920802374
2211	20	-0.09395992342407311
2211	21	0.039387534731567087
2211	22	-0.002582786034267315
2211	23	0.11795999765563892
2211	24	0.14431243582713194
2211	25	0.05515330529251372
2212	1	0.18304829629787278
2212	2	0.09970413770163233
2212	3	0.18606692433743532
2212	4	0.21247181869162546
2212	5	0.2681927921784535
2212	6	0.251433667379267
2212	7	0.17736551010597168
2212	8	0.32493868812937166
2212	9	0.11603268898806363
2212	10	0.31428728691025754
2212	11	0.16274055252956998
2212	12	0.27996060961740454
2212	13	0.15070003234193052
2212	14	0.1925378410053895
2212	15	0.22806874648452435
2212	16	0.1213709803512728
2212	17	0.19373119081190282
2212	18	0.3435088495122329
2212	19	0.28324951551408617
2212	20	0.2500557040404843
2212	21	0.24504146490868806
2212	22	-0.2861403091253152
2212	23	0.2197679454018342
2212	24	0.34453667079871764
2212	25	0.43753411797182906
2213	1	-0.09462749457880193
2213	2	-0.7806135939768417
2213	3	-0.16851413161409923
2213	4	-0.0029443682083538647
2213	5	0.035814130402663125
2213	6	0.03196015351544901
2213	7	-0.016558140665758003
2213	8	0.19542391367524203
2213	9	-0.10796279559604471
2213	10	-1.4743034371522938
2213	11	-0.43257117885816854
2213	12	-0.167298728233213
2213	13	-0.03930203261657539
2213	14	0.14444872918738128
2213	15	0.02960068874823918
2213	16	-0.038569176679217776
2213	17	-0.11323359997873439
2213	18	-0.007678752586258497
2213	19	-0.08431927919851034
2213	20	-0.05188565286086799
2213	21	-0.0828672789826328
2213	22	0.09188695918928147
2213	23	-0.7831150558020425
2213	24	-1.5116381638032763
2213	25	-1.299854250007897
2214	1	-0.012159575987116256
2214	2	-0.1734511780692749
2214	3	-0.021236073997446425
2214	4	-0.003295008941556432
2214	5	-0.04864553793289872
2214	6	-0.09294782333562779
2214	7	0.06404339818578109
2214	8	-0.002777187119633595
2214	9	-0.2701764032607201
2214	10	0.03187873597898516
2214	11	-0.04799123437447861
2214	12	-0.04458215341630023
2214	13	9.973757130765648E-4
2214	14	-0.014106028380524549
2214	15	-0.03336551929988474
2214	16	-0.11730023385711519
2214	17	-0.10207257455204655
2214	18	0.05695649491061516
2214	19	-0.7247542676172642
2214	20	-0.2547759721561178
2214	21	-0.180429380577678
2214	22	-0.47612779673336547
2214	23	-1.699264748332297
2214	24	-1.188508539199546
2214	25	-1.5275426217946233
2215	1	-0.42557798429785265
2215	2	-0.21565771343331974
2215	3	-1.1186612945874899
2215	4	-0.763116591127222
2215	5	-0.2600484250612496
2215	6	-0.16644762670940594
2215	7	-0.1592799536358811
2215	8	-0.09630265431172802
2215	9	-0.3194619946550503
2215	10	-1.240995447817376
2215	11	-0.4935739034115869
2215	12	-0.45473267488542746
2215	13	-0.5432066567630187
2215	14	-0.8418604340368034
2215	15	-1.1362163946962127
2215	16	-0.7077820909917776
2215	17	-0.4038441563280699
2215	18	-0.35909639754303413
2215	19	-0.9784225755666967
2215	20	-1.3076033957791182
2215	21	-0.5780413960783832
2215	22	-0.26498707790067044
2215	23	-0.47033703714344455
2215	24	-1.678757107514869
2215	25	-0.8123551919451231
2216	1	-0.5876743154482149
2216	2	-0.38520297286364524
2216	3	-1.3510387984177967
2216	4	-0.6134615394225165
2216	5	-0.42083130886609865
2216	6	-0.24106623015826756
2216	7	-0.23633194922204837
2216	8	-0.38678067995858356
2216	9	-0.16504533750771344
2216	10	-0.23623686010191136
2216	11	-0.3862557236810602
2216	12	-0.16201439163949516
2216	13	-1.0287804488772423
2216	14	-0.6378466213843694
2216	15	-1.0864705609261662
2216	16	-0.8433214662957553
2216	17	-1.8081753602746105
2216	18	-0.9124150730640608
2216	19	-0.46657659800563067
2216	20	-0.44943841236631243
2216	21	-0.25919467424275666
2216	22	-0.3765656995559965
2216	23	-0.27868140603512614
2216	24	-0.329109106987282
2216	25	-0.304456033493544
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	4046.0
3	432.0
4	511.0
5	435.0
6	292.0
7	269.0
8	455.0
9	1012.0
10	1991.0
11	3664.0
12	6104.0
13	8969.0
14	11960.0
15	15073.0
16	17469.0
17	18502.0
18	19046.0
19	19703.0
20	20125.0
21	21470.0
22	23216.0
23	24524.0
24	25657.0
25	27087.0
26	28601.0
27	31650.0
28	34766.0
29	41027.0
30	49278.0
31	62319.0
32	82428.0
33	113512.0
34	162876.0
35	256229.0
36	461666.0
37	1507838.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.191586758603215	21.381387824848332	13.752190106760231	22.674835309788218
2	14.72430572184468	21.31848790100062	40.76682826204397	23.190378115110725
3	18.219090004144444	26.59906446610917	30.29207352578528	24.88977200396111
4	11.752170002408423	18.158447141562995	38.65316184669901	31.436221009329575
5	12.341840211062708	38.88604357527828	35.534777231319694	13.237338982339322
6	30.107887835384307	38.79601371797458	17.76087149485576	13.335226951785353
7	25.11737240733341	33.485623211494975	23.70251283140397	17.694491549767644
8	23.749931801401807	37.80772095296605	21.390211878920613	17.05213536671153
9	23.63471130409517	17.767525947926586	22.472244192991234	36.12551855498701
10	14.331676079725103	31.1820999821457	34.10694435431198	20.379279583817222
11	31.234109114642255	26.625707978584163	23.007080834878106	19.133102071895475
12	20.204917635893512	28.858458985806195	31.860431334191997	19.076192044108296
13	26.102314235184043	24.623479146313827	26.750559656077055	22.523646962425076
14	19.798395674797618	24.690073540449184	28.57318099485724	26.93834978989596
15	23.044454034554736	31.09674674695676	24.201421581427837	21.657377637060662
16	20.653856297000704	32.29103419380308	26.700708434165847	20.354401075030367
17	19.679769189526116	31.20715517633741	28.96577191455132	20.147303719585157
18	19.751620243415	30.600803076469163	29.96555338450667	19.682023295609163
19	20.232383602413897	29.813857474188115	30.362294396679033	19.591464526718948
20	21.558598504692085	29.277480874512722	29.42202149646347	19.741899124331724
21	21.46993480950412	29.798192534837714	28.794238886355117	19.937633769303044
22	20.36449803002827	29.53404987840597	29.499285671401097	20.60216642016466
23	20.077006955029564	30.050466412430087	29.401156008162943	20.471370624377403
24	20.349549745008325	29.672469034880073	29.423676193501812	20.554305026609793
25	21.02625772179115	29.129945618574588	28.236857015107024	21.606939644527234
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7443.0
1	7443.0
2	6352.0
3	5261.0
4	5261.0
5	5261.0
6	7401.5
7	9542.0
8	9542.0
9	9542.0
10	13222.5
11	16903.0
12	16903.0
13	16903.0
14	26423.0
15	35943.0
16	35943.0
17	35943.0
18	54762.0
19	73581.0
20	73581.0
21	73581.0
22	102905.0
23	132229.0
24	132229.0
25	132229.0
26	168059.0
27	203889.0
28	203889.0
29	203889.0
30	239944.5
31	276000.0
32	276000.0
33	276000.0
34	303413.5
35	330827.0
36	330827.0
37	330827.0
38	346210.5
39	361594.0
40	361594.0
41	361594.0
42	357994.5
43	354395.0
44	354395.0
45	354395.0
46	369616.5
47	384838.0
48	384838.0
49	384838.0
50	351965.5
51	319093.0
52	319093.0
53	319093.0
54	277346.5
55	235600.0
56	235600.0
57	235600.0
58	200602.5
59	165605.0
60	165605.0
61	165605.0
62	134558.5
63	103512.0
64	103512.0
65	103512.0
66	78162.5
67	52813.0
68	52813.0
69	52813.0
70	37620.0
71	22427.0
72	22427.0
73	22427.0
74	15443.0
75	8459.0
76	8459.0
77	8459.0
78	5716.5
79	2974.0
80	2974.0
81	2974.0
82	1946.0
83	918.0
84	918.0
85	918.0
86	589.0
87	260.0
88	260.0
89	260.0
90	162.5
91	65.0
92	65.0
93	65.0
94	40.0
95	15.0
96	15.0
97	15.0
98	15.5
99	16.0
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3093870824128069
2	0.17769462167732641
3	0.1957991135886131
4	0.21712504534176577
5	0.24135027295259778
6	0.19576689919019444
7	0.19148238420051272
8	0.21361367591413186
9	0.21429017828092373
10	0.22256927867451926
11	0.19718433272061545
12	0.20527014672369906
13	0.2178981909038136
14	0.20781508419877318
15	0.1966044735490796
16	0.20111448932769194
17	0.2013077757182039
18	0.2050446459347684
19	0.20317621082648618
20	0.19686218873642886
21	0.19492932483130931
22	0.19934269741466565
23	0.20295071003755555
24	0.2205397715741437
25	0.21380696230464385
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3104202.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.77435669590066
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.93850217784214	75.18211871177704
2	6.530717809957712	10.68090495343506
3	0.8109458041514602	1.9894371434917655
4	0.24094775286729997	0.7881338995218524
5	0.1026661530619301	0.4197729310541103
6	0.07148543236561354	0.3507405146895818
7	0.052336114911790736	0.29958264902120946
8	0.03211702942425834	0.2101079536121631
9	0.029901329814629382	0.2200645808948945
>10	0.16357767422262118	2.491994724352003
>50	0.013751753055661809	0.7988775023334407
>100	0.010591314941609253	1.6106047660750455
>500	7.140929186430335E-4	0.4032788294257236
>1k	0.0015075294921145712	2.72851493685808
>5k	1.5868731495942854E-4	0.8142462654293504
>10k+	7.934365747971427E-5	1.0116196380287437
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	16411	0.5286704924486229	No Hit
CCCATGTACTCTGCGTTGATACCAC	14772	0.47587109344043976	No Hit
GAGTACATGGGAAGCAGTGGTATCA	7853	0.25297967078173395	No Hit
GTATCAACGCAGAGTACTTTTTTTT	6143	0.19789304948582598	No Hit
CATGTACTCTGCGTTGATACCACTG	5678	0.18291335422114927	No Hit
GGTATCAACGCAGAGTACTTTTTTT	5425	0.17476311142122838	No Hit
GCTTCCCATGTACTCTGCGTTGATA	4997	0.16097534889804208	No Hit
GCGTTGATACCACTGCTTCCCATGT	4766	0.1535338228633317	No Hit
GTATCAACGCAGAGTACATGGGAAG	4639	0.14944259426416193	No Hit
GGTATCAACGCAGAGTACATGGGAA	4394	0.14155006665159034	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	4314	0.13897291477809756	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4195	0.13513940136627706	No Hit
TATCAACGCAGAGTACATGGGAAGC	3807	0.12264021477983714	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	3679	0.1185167717822487	No Hit
ATTCCATTCCATTCCATTCCATTCC	3454	0.1112685321380503	No Hit
ACTCTGCGTTGATACCACTGCTTCC	3306	0.10650080117208867	No Hit
GTGGTATCAACGCAGAGTACATGGG	3198	0.10302164614287344	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.221439841865961E-5	0.0
3	0.0	0.0	0.0	3.221439841865961E-5	0.0
4	0.0	0.0	0.0	3.221439841865961E-5	0.0
5	0.0	0.0	0.0	3.221439841865961E-5	0.0
6	0.0	0.0	0.0	3.221439841865961E-5	0.0
7	0.0	0.0	0.0	6.442879683731922E-5	0.0
8	0.0	0.0	0.0	6.442879683731922E-5	0.0
9	0.0	0.0	0.0	9.664319525597883E-5	0.0
10	0.0	0.0	0.0	1.6107199209329805E-4	0.0
11	0.0	0.0	0.0	1.6107199209329805E-4	0.0
12	0.0	0.0	0.0	1.9328639051195766E-4	0.0
13	0.0	0.0	0.0	1.9328639051195766E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAACGC	45	6.734992E-4	14.7864485	3
TATACCG	55	1.950337E-4	13.827185	5
GACGGTG	55	0.003073597	12.088823	7
AACTGCG	55	0.003073597	12.088823	7
GCGTAGT	60	0.0058269887	11.096292	1
GTCTAGG	340	0.0	10.070584	1
GTATAAG	470	0.0	9.511107	1
GTCTTAC	340	0.0	9.511107	1
GTTAGAC	170	1.6507329E-8	9.505574	3
TAGGACT	370	0.0	9.249563	4
CCTACAC	345	0.0	9.092289	3
ACTGCGT	115	1.1133257E-4	9.085389	8
GTTCTAG	360	0.0	8.982712	1
CCAGGAC	340	0.0	8.946424	3
GTCTTAG	300	0.0	8.877033	1
CTATACT	495	0.0	8.834319	4
CTTACTC	390	0.0	8.774376	3
GTCCTAC	240	1.0004442E-10	8.718515	1
GGAACGA	120	1.7701488E-4	8.705848	15
GTCTAGA	275	1.8189894E-12	8.6464615	1
>>END_MODULE