FastQCFastQC Report
Wed 25 May 2016
SRR1294991_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294991_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3104202
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC168450.5426515413623211No Hit
CCCATGTACTCTGCGTTGATACCAC166630.5367885208501251No Hit
GTATCAACGCAGAGTACTTTTTTTT143820.4633074780571625No Hit
GGTATCAACGCAGAGTACTTTTTTT131210.42268512165123273No Hit
TATCAACGCAGAGTACTTTTTTTTT100100.3224661281707827No Hit
GAGTACATGGGAAGCAGTGGTATCA88820.28612828675453467No Hit
CATGTACTCTGCGTTGATACCACTG63270.20382049879485936No Hit
GCTTCCCATGTACTCTGCGTTGATA56370.18159256388598424No Hit
ACGCAGAGTACTTTTTTTTTTTTTT55480.17872548242672354No Hit
ACGCAGAGTACATGGGAAGCAGTGG54340.1750530410069963No Hit
GCGTTGATACCACTGCTTCCCATGT51310.16529207828614245No Hit
GTATCAACGCAGAGTACATGGGAAG48260.1554666867684513No Hit
GGTATCAACGCAGAGTACATGGGAA47590.1533083220744011No Hit
TATCAACGCAGAGTACATGGGAAGC43530.1402292763164253No Hit
ACTCTGCGTTGATACCACTGCTTCC38460.12389657631816486No Hit
ATTCCATTCCATTCCATTCCATTCC33950.10936788263134938No Hit
GTGGTATCAACGCAGAGTACATGGG32260.10392364929859589No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG550.003071151412.090115
CTAGGAC3050.011.2123733
CTGTGCG600.00588079211.08269
CTTACAC4100.010.88952353
CAATCCG1106.055112E-610.36295110
GTATTAG4250.010.2947731
GTACGGA856.5240817E-410.0709731
TATACTG6750.09.9919325
GTCTAAG3150.09.9644031
CTAGACA3650.09.8897574
ACTGATC3050.09.6552548
CCTACAC3950.09.61961653
TTATACT5350.09.5881514
GACTCCG1002.7582116E-49.4995257
CTACACC2800.09.49937254
CCAGGAC3450.09.36173
TCAGGAC3250.09.3532283
GTCTAGG3000.09.1944261
GTCCTAG3350.09.08558751
TAGACTG4500.09.0771775
Produced by FastQC (version 0.11.5)