FastQCFastQC Report
Wed 25 May 2016
SRR1294990_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294990_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3561236
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC291050.8172724301338076No Hit
CCCATGTACTCTGCGTTGATACCAC255300.7168859351079232No Hit
GAGTACATGGGAAGCAGTGGTATCA145200.40772361056666845No Hit
CATGTACTCTGCGTTGATACCACTG101140.28400252047322894No Hit
GCTTCCCATGTACTCTGCGTTGATA91170.2560066224198565No Hit
GCGTTGATACCACTGCTTCCCATGT83740.23514307953755384No Hit
GTATCAACGCAGAGTACATGGGAAG77850.21860387797944308No Hit
ACGCAGAGTACATGGGAAGCAGTGG75700.21256664820865565No Hit
GGTATCAACGCAGAGTACATGGGAA72200.20273859974458303No Hit
TATCAACGCAGAGTACATGGGAAGC69260.19448303903476208No Hit
GTATCAACGCAGAGTACTTTTTTTT67210.18872661064866242No Hit
GGTATCAACGCAGAGTACTTTTTTT62800.17634326958393096No Hit
ACTCTGCGTTGATACCACTGCTTCC61220.1719066077058639No Hit
TATCAACGCAGAGTACTTTTTTTTT46380.13023568221819615No Hit
GTGGTATCAACGCAGAGTACATGGG44600.12523741757075352No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN41940.11776810073805836No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC2550.011.5548224
AGACCGT752.0780468E-411.3983456
GTACTAG1909.094947E-1210.5126771
TACACCG1201.5160513E-610.2965485
TAGACAG4800.010.0985375
CTAGGAC2750.010.0226523
TATTCCG1054.0869007E-59.9571015
ACTAGAC2106.548362E-119.5042383
GTCTAGG3950.09.3910711
CGGTGAA1554.2712418E-79.1926019
GTGTTAG4450.09.1908581
TTACACC3950.09.1438334
ACACCGT1054.5015945E-49.0463066
TAGACTG5150.09.0431225
CCTGGAC5500.08.9858263
CCTATAC3500.08.9611393
CGCAAAA1701.6753074E-78.9451652
CTTACAC5650.08.915483
GACTCCG1401.0873726E-58.820527
GTATTAG4900.08.7350231
Produced by FastQC (version 0.11.5)