##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294990_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3561236
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.068144599234646	33.0	33.0	33.0	27.0	33.0
2	31.023122590022115	33.0	33.0	33.0	27.0	33.0
3	30.994400820389327	33.0	33.0	33.0	27.0	33.0
4	34.41931621493212	37.0	37.0	37.0	27.0	37.0
5	34.78235309313957	37.0	37.0	37.0	33.0	37.0
6	34.791820311824324	37.0	37.0	37.0	33.0	37.0
7	34.64558962113154	37.0	37.0	37.0	27.0	37.0
8	34.55095843128622	37.0	37.0	37.0	27.0	37.0
9	34.44360413070069	37.0	37.0	37.0	27.0	37.0
10	34.35784457980319	37.0	37.0	37.0	27.0	37.0
11	34.5588919128078	37.0	37.0	37.0	27.0	37.0
12	34.458972390484654	37.0	37.0	37.0	27.0	37.0
13	34.37626206182348	37.0	37.0	37.0	27.0	37.0
14	35.73827317257267	37.0	37.0	40.0	27.0	40.0
15	35.90689243846799	40.0	37.0	40.0	27.0	40.0
16	36.014967275406626	40.0	37.0	40.0	27.0	40.0
17	35.959542136494186	40.0	37.0	40.0	27.0	40.0
18	35.90047331881403	40.0	37.0	40.0	27.0	40.0
19	35.982214601896644	40.0	37.0	40.0	27.0	40.0
20	35.96477852071584	40.0	37.0	40.0	27.0	40.0
21	35.95695932535782	40.0	37.0	40.0	27.0	40.0
22	35.88704483499549	40.0	37.0	40.0	27.0	40.0
23	35.81113888548807	37.0	37.0	40.0	27.0	40.0
24	35.8623354363485	37.0	37.0	40.0	27.0	40.0
25	34.73236118021945	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.3757799253342249
1101	2	0.3845591995325286
1101	3	0.31877919819808653
1101	4	0.37800215647219204
1101	5	0.2859509277093082
1101	6	0.1927476514654245
1101	7	0.10593147509676015
1101	8	0.20052789808284643
1101	9	0.2724864443239454
1101	10	0.4823646459495592
1101	11	0.4108183714514837
1101	12	0.40156999410254457
1101	13	0.3286445624934373
1101	14	0.7299237012080511
1101	15	0.645872332236678
1101	16	0.6070165183625491
1101	17	0.6480161002000102
1101	18	0.612501630271332
1101	19	0.487138636218269
1101	20	0.4695274906030207
1101	21	0.4314384140091576
1101	22	0.5399816908374788
1101	23	0.6436707228444618
1101	24	0.6211181180500844
1101	25	0.7291452172180399
1102	1	0.2853136474746556
1102	2	0.3009005201434647
1102	3	0.2808720799535749
1102	4	0.3837397350459355
1102	5	0.20841282581478282
1102	6	0.18249691343161345
1102	7	0.2248127998227929
1102	8	0.30755687985259783
1102	9	0.33699136397544294
1102	10	0.5371196068019515
1102	11	0.3851672186724997
1102	12	0.4373603990539223
1102	13	0.44593590852100107
1102	14	0.7851447477540816
1102	15	0.6893444129519963
1102	16	0.7352829458005274
1102	17	0.6115970645760385
1102	18	0.8422424073155668
1102	19	0.6937291945617545
1102	20	0.7210166747527467
1102	21	0.6220714615360876
1102	22	0.6534289406782605
1102	23	0.7684891602880981
1102	24	0.625280972716105
1102	25	0.7560100515152826
1103	1	0.5053878179362634
1103	2	0.4365642782936163
1103	3	0.4429170392559527
1103	4	0.4609565600820318
1103	5	0.2956114567309811
1103	6	0.20419700361141224
1103	7	0.30055561791565566
1103	8	0.5013676504617948
1103	9	0.4256190628204948
1103	10	0.6052145027297726
1103	11	0.42770043236721733
1103	12	0.5988860873180144
1103	13	0.5331399568426605
1103	14	0.7953898977127736
1103	15	0.7487480816844112
1103	16	0.7101530562330609
1103	17	0.8077913117931885
1103	18	0.8000062419602045
1103	19	0.76447745569277
1103	20	0.7604327351638673
1103	21	0.7551277751749197
1103	22	0.79632377164085
1103	23	0.9286793672115365
1103	24	0.8380230302997802
1103	25	0.8610826663816411
1104	1	0.23434891228404808
1104	2	0.15127228334724663
1104	3	0.304730452032306
1104	4	0.20857716030301532
1104	5	0.15982988467445836
1104	6	0.06667607814534193
1104	7	0.07256505919391998
1104	8	0.23942552859594457
1104	9	0.16055515790282016
1104	10	0.27904045520463683
1104	11	0.20957317811067355
1104	12	0.291362749001685
1104	13	0.27078001122527695
1104	14	0.5854191101174919
1104	15	0.47626167956466503
1104	16	0.28417251751749717
1104	17	0.4422799344474484
1104	18	0.473457748567057
1104	19	0.40062437423369346
1104	20	0.4072041367449515
1104	21	0.30688850590350114
1104	22	0.26666499852623105
1104	23	0.3915475962061592
1104	24	0.4534958287519899
1104	25	0.5790317614737575
1105	1	0.29755505445002584
1105	2	0.24440551332286375
1105	3	0.2135247934180704
1105	4	0.24894787153879605
1105	5	0.15583260597659176
1105	6	0.05812045954765921
1105	7	0.17928401062785326
1105	8	0.24289524175623
1105	9	0.3089205147737104
1105	10	0.3610454654359927
1105	11	0.24591329146296914
1105	12	0.31107852999510754
1105	13	0.28978652417308126
1105	14	0.5540188131037809
1105	15	0.33939521837807973
1105	16	0.3199179681162505
1105	17	0.32316508120275955
1105	18	0.3456296459164321
1105	19	0.27412649771410713
1105	20	0.30265594437253895
1105	21	0.3819127584939892
1105	22	0.29119437826523864
1105	23	0.4284689952237031
1105	24	0.42774207482865023
1105	25	0.29001927472069866
1106	1	0.001926448320194396
1106	2	-0.17489761874928433
1106	3	-0.18199139670560172
1106	4	-0.05545065236758262
1106	5	-0.37194607259028345
1106	6	-0.23030536685172365
1106	7	-0.6030883893392343
1106	8	-0.16031334510800832
1106	9	0.12587443504538953
1106	10	-0.26218482100480145
1106	11	-0.4137888985082867
1106	12	-0.751006843687378
1106	13	-0.18980476509655375
1106	14	-0.38613059058084787
1106	15	-0.17385787109560624
1106	16	-0.03547285393201349
1106	17	-0.23545349534746407
1106	18	-0.4177502816710117
1106	19	-0.5501113846173808
1106	20	-0.19366858874187187
1106	21	-0.03127847212252277
1106	22	0.2491653810354535
1106	23	-0.6884775266218028
1106	24	0.014453707403063731
1106	25	0.24665024960842175
1107	1	-1.0171152123981386
1107	2	-1.1003732897301148
1107	3	-0.8747007763801555
1107	4	-0.9388974723407983
1107	5	-0.9069404960513836
1107	6	-0.869594704993105
1107	7	-0.9564393046143849
1107	8	-0.9446252086768538
1107	9	-0.9197843878616894
1107	10	-0.8859214329511147
1107	11	-0.8868709283583982
1107	12	-0.8198564459512099
1107	13	-0.7987294380028871
1107	14	-1.1086820076763146
1107	15	-0.8686429405416263
1107	16	-0.8836292513909854
1107	17	-1.044313111623886
1107	18	-1.1409079800472526
1107	19	-1.0342846583288647
1107	20	-1.1142198661636868
1107	21	-1.0644806442847283
1107	22	-0.9514082858006745
1107	23	-0.8630206103821365
1107	24	-0.7502489725295831
1107	25	-0.7080906769332174
1108	1	0.0338844756675627
1108	2	-0.11339376409285506
1108	3	0.015261760110877987
1108	4	-0.2143557203773696
1108	5	-0.3816636864685776
1108	6	-0.0249419809048419
1108	7	0.02255578748614795
1108	8	0.21625697462350502
1108	9	-0.1296448222351998
1108	10	-0.4956459632873731
1108	11	-0.055625671229499574
1108	12	0.16354710827327068
1108	13	0.2075315085935614
1108	14	-0.36101916042109394
1108	15	-0.02445797520400106
1108	16	-0.6835164070895772
1108	17	-0.68882702066864
1108	18	-0.5500501949748369
1108	19	-0.04401398326366035
1108	20	-0.9931037407015992
1108	21	-0.20341640893615676
1108	22	-0.2999145257709017
1108	23	-0.12116893071726054
1108	24	-0.2785774870059896
1108	25	-0.9473071824528532
1109	1	0.15897022315089515
1109	2	0.10807363906049972
1109	3	0.24822967046628008
1109	4	0.21653185444351664
1109	5	0.15972620856740605
1109	6	0.12139745276932956
1109	7	0.22183321707857573
1109	8	0.290942499149466
1109	9	0.2568949984600195
1109	10	0.22663897497351115
1109	11	0.18605531529007635
1109	12	0.29620219919204516
1109	13	0.1779926254845705
1109	14	0.5184649203742353
1109	15	0.3985135552399015
1109	16	0.3522527949193375
1109	17	0.35307619957728775
1109	18	0.31334471697314115
1109	19	0.3299505294781824
1109	20	0.33809195913053713
1109	21	0.3468597735719712
1109	22	0.36250257262518204
1109	23	0.4497809073992869
1109	24	0.5610112145326482
1109	25	0.5277005689155345
1110	1	0.18397429963759393
1110	2	0.1111229056047236
1110	3	0.13646330410634988
1110	4	-0.9488289741189817
1110	5	-0.1536772978590264
1110	6	0.039269036868752494
1110	7	0.07842722873323993
1110	8	0.17548979599223458
1110	9	0.18840199914852462
1110	10	0.09317473994554604
1110	11	0.08745365467501642
1110	12	0.1731000209706508
1110	13	0.18740030303637667
1110	14	0.3442781726585977
1110	15	0.2645242360286204
1110	16	0.1640353945668096
1110	17	0.1982938918383539
1110	18	0.3669620062902297
1110	19	0.1976475709206511
1110	20	0.2479932473003288
1110	21	0.3724527532836319
1110	22	-0.2347193190983674
1110	23	0.24367140881895466
1110	24	0.3852207560606544
1110	25	0.44985060215411465
1111	1	-0.027692102754688364
1111	2	-0.2503366403883369
1111	3	-0.15230583024892397
1111	4	-0.10933167634325258
1111	5	-0.22296600313081427
1111	6	-0.06585162555032298
1111	7	0.040470038910086714
1111	8	0.07106393440913195
1111	9	0.08634742808858675
1111	10	-0.04389054851002072
1111	11	-0.26021761838239854
1111	12	-0.0540664105411679
1111	13	-0.08996782202705589
1111	14	-0.2048190573719566
1111	15	-0.06335448598891702
1111	16	-0.10323685079301015
1111	17	-0.038238292219972436
1111	18	-0.10473429694636849
1111	19	-0.09347050953619629
1111	20	-0.1562065407836215
1111	21	-0.15824436903903205
1111	22	-0.026359681714524186
1111	23	0.07670282325105404
1111	24	0.024469740608395796
1111	25	0.031191370945194308
1112	1	-0.10704404052864547
1112	2	-0.370802309687992
1112	3	-0.08582961390628796
1112	4	-0.09186710097866779
1112	5	-0.05156677718418479
1112	6	-0.07008716108812507
1112	7	-0.04523918560311557
1112	8	0.10676614264910711
1112	9	-0.07257816169330766
1112	10	0.12296297735588269
1112	11	-0.022102898542939897
1112	12	-0.020058639829962033
1112	13	0.05944104563698005
1112	14	-0.19935484413760918
1112	15	-0.09739255492945631
1112	16	-0.07520848392491075
1112	17	-0.20740809357645418
1112	18	-0.10476190937648511
1112	19	-0.07033132327300251
1112	20	-0.7222885481921963
1112	21	-1.106983325953081
1112	22	-1.600528902153762
1112	23	-0.4937118309523001
1112	24	-0.26090928148909853
1112	25	-1.3374372267809278
1113	1	-0.1941476102136903
1113	2	-0.12738896294183277
1113	3	-0.16853843234497745
1113	4	-0.5469144551511889
1113	5	-0.225305079179833
1113	6	-0.12170856440484101
1113	7	-0.22375870570016332
1113	8	-0.03072481109144576
1113	9	-0.13234880240221258
1113	10	0.01718603459215018
1113	11	-0.07219393808611585
1113	12	-0.1510465239076595
1113	13	-0.20416958797102325
1113	14	-0.4339159637139218
1113	15	-0.36563321649727953
1113	16	-0.39391339686483207
1113	17	-0.43643062416212786
1113	18	-0.7697399409459322
1113	19	-1.0074887119145117
1113	20	-1.0698777607312167
1113	21	-0.9292727766683484
1113	22	-0.4259802091644218
1113	23	-1.7503093722633025
1113	24	-0.9256457047031432
1113	25	-0.4288073171695572
1114	1	-0.3308250139312996
1114	2	-0.446964929676696
1114	3	-0.31869202196688917
1114	4	-0.4497696433826235
1114	5	-0.6087563318418319
1114	6	-0.34113713652206457
1114	7	-0.39722921251578214
1114	8	-0.09891878112277652
1114	9	-1.1702988419165266
1114	10	-0.21546819734180644
1114	11	-0.379650135044443
1114	12	-0.34143381124240335
1114	13	-0.3157000662155909
1114	14	-0.7144729298914925
1114	15	-1.3775813396061025
1114	16	-1.1021090977097643
1114	17	-1.517024760365338
1114	18	-1.7023787814936426
1114	19	-1.5265744612755938
1114	20	-0.7910226847501676
1114	21	-0.5684791423896058
1114	22	-0.4655890120043793
1114	23	-0.2679942013204979
1114	24	-0.8332014636152678
1114	25	-0.49126024847972616
1115	1	-0.7030214248203706
1115	2	-0.47615516148789894
1115	3	-0.503345275430366
1115	4	-0.6120667269721238
1115	5	-0.4300729716675846
1115	6	-0.1991233345336596
1115	7	-0.28586500374242974
1115	8	-0.10946200134341666
1115	9	-0.4227848003094792
1115	10	-0.10438823816538445
1115	11	-0.3510231883980168
1115	12	-0.45773091659480514
1115	13	-0.3007578206556829
1115	14	-0.3279481120197971
1115	15	-1.5262257713857466
1115	16	-0.8138942599884871
1115	17	-0.6251737551005263
1115	18	-2.143816081887003
1115	19	-1.0022731185044194
1115	20	-0.7797510306606412
1115	21	-0.8197536041204501
1115	22	-0.640537200423978
1115	23	-0.26639936017156174
1115	24	-0.4152964367018086
1115	25	-0.47804755871173654
1116	1	-1.7352587062430551
1116	2	-0.9602240473608923
1116	3	-1.84280021543087
1116	4	-1.1292848158557263
1116	5	-1.1114719791256178
1116	6	-0.6780911255825757
1116	7	-1.3159201166762955
1116	8	-3.029743236586853
1116	9	-1.6875521738491486
1116	10	-0.9050754432594843
1116	11	-0.7690936431974364
1116	12	-0.7656960343408841
1116	13	-1.9625857438512782
1116	14	-1.9032525251998607
1116	15	-1.7651913568359134
1116	16	-1.2369833531111638
1116	17	-1.983705547263284
1116	18	-2.851514376254009
1116	19	-1.6079140893824047
1116	20	-1.15825374226295
1116	21	-1.005693407720777
1116	22	-0.7650452775564034
1116	23	-0.7317436926614036
1116	24	-0.8391721333015383
1116	25	-0.8463102317802722
1201	1	0.36117867550003524
1201	2	0.3753046040682442
1201	3	0.42005089424850794
1201	4	0.3075590027486399
1201	5	0.21319344251310213
1201	6	0.10364911831315737
1201	7	0.19915844971615826
1201	8	0.25341374652315807
1201	9	0.2510800497024448
1201	10	0.5755841672431004
1201	11	0.3513434139806435
1201	12	0.47621807812735284
1201	13	0.4435589442575818
1201	14	0.854943759694514
1201	15	0.7764864438546581
1201	16	0.7578606536374579
1201	17	0.6776160982813337
1201	18	0.6593964068366702
1201	19	0.48893562570188465
1201	20	0.6050937837854917
1201	21	0.5632611082425498
1201	22	0.6647699951671058
1201	23	0.7055715533921756
1201	24	0.6976960600060167
1201	25	0.887461016479314
1202	1	0.3434570619979098
1202	2	0.3973668902648768
1202	3	0.4268840153418054
1202	4	0.489204925145323
1202	5	0.24577477039278506
1202	6	-0.02540275300180639
1202	7	-0.002076499814769761
1202	8	0.16409462823209964
1202	9	0.14608985157067877
1202	10	0.2872009211017783
1202	11	0.14725884799377553
1202	12	0.1934420755498607
1202	13	0.12902753926589128
1202	14	0.47030941548635496
1202	15	0.3054149658165528
1202	16	0.29369838203816556
1202	17	0.3589052259542598
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2210	22	0.6835501218098088
2210	23	0.6936303397937564
2210	24	0.7071292049908706
2210	25	0.7682020077121265
2211	1	0.13436501376979137
2211	2	0.01854753758340877
2211	3	0.0945385759938695
2211	4	0.10369595845631352
2211	5	0.14662461226614454
2211	6	0.05743061165780006
2211	7	0.14688858972627372
2211	8	0.2442792651511212
2211	9	0.11249874969371376
2211	10	0.3459908636224682
2211	11	0.12008976744493083
2211	12	0.10767547563590085
2211	13	0.05822055417002758
2211	14	-0.0032463384463738976
2211	15	0.03129403990664059
2211	16	0.023180586482219212
2211	17	0.14329074994132185
2211	18	0.13892029356259172
2211	19	0.06207036054080817
2211	20	0.1048528891282885
2211	21	0.1267795723950158
2211	22	0.12050405986924773
2211	23	0.3083021197794338
2211	24	0.34360096880344315
2211	25	0.22548610498206045
2212	1	0.15680088134923764
2212	2	0.24352926463688362
2212	3	0.14040279662414434
2212	4	0.21242475173696818
2212	5	0.191005370454441
2212	6	0.2616016494039286
2212	7	0.3125276658209444
2212	8	0.40112641218332357
2212	9	0.24291264594989315
2212	10	0.43083367178290644
2212	11	0.33001214799082845
2212	12	0.3534036644957226
2212	13	0.2562588000597188
2212	14	0.3157969939647316
2212	15	0.20297118341864717
2212	16	0.2638044244910276
2212	17	0.3900679088738599
2212	18	0.28556541380626044
2212	19	0.3032958604191478
2212	20	0.25992180953846145
2212	21	0.2293203945343265
2212	22	-0.33623855505095435
2212	23	0.2233174177911792
2212	24	0.1973013124161227
2212	25	0.38847233312253593
2213	1	-0.09141743351808174
2213	2	-0.7495128662039185
2213	3	-0.19804240026286024
2213	4	-7.186531511251815E-4
2213	5	0.03840466342361282
2213	6	-0.07094022403209266
2213	7	0.13546930812863422
2213	8	0.21587417733874759
2213	9	-0.14504714003407315
2213	10	-1.362903290312154
2213	11	-0.3872396378912768
2213	12	-0.048238247319901006
2213	13	-0.011509374909515202
2213	14	0.01147424983528822
2213	15	-0.014158748511583497
2213	16	0.0328065656836003
2213	17	-0.0709045418107408
2213	18	0.09106109793042094
2213	19	0.08983898716149241
2213	20	-0.010229337570315522
2213	21	-0.02266484369738464
2213	22	0.030150160586835284
2213	23	-0.8524370884371564
2213	24	-1.4611730273308439
2213	25	-1.3244972944751154
2214	1	-0.11277852103554409
2214	2	-0.27724423940806275
2214	3	-0.038076777665555284
2214	4	-0.027415483908839633
2214	5	-0.01398867202979659
2214	6	-0.15639866320140072
2214	7	-0.042025166931644264
2214	8	0.012522179303168457
2214	9	-0.3718856668778301
2214	10	0.004902714864584823
2214	11	-0.11493156161692042
2214	12	-0.0823298425682566
2214	13	-0.06345789736552376
2214	14	-0.007250163353724304
2214	15	-0.2886164188186058
2214	16	-0.292202793503904
2214	17	-0.27581237897295097
2214	18	-0.08704927204933455
2214	19	-0.8415108693309676
2214	20	-0.4638661366879333
2214	21	-0.40759111745261123
2214	22	-0.6387479510712879
2214	23	-1.7965214781174907
2214	24	-1.3054260836590643
2214	25	-1.7498733727944042
2215	1	-0.4166789093040002
2215	2	-0.2722783445854873
2215	3	-1.1761585551334441
2215	4	-0.8935281069744292
2215	5	-0.3559940202100691
2215	6	-0.2806586614651039
2215	7	-0.19966477710134
2215	8	-0.16298304056275725
2215	9	-0.3853661558052508
2215	10	-1.2856750867664744
2215	11	-0.5301485668731019
2215	12	-0.6089040783823592
2215	13	-0.5567084535193061
2215	14	-0.8154957266278231
2215	15	-1.0911647159175075
2215	16	-0.6834519047413394
2215	17	-0.5027609819547223
2215	18	-0.37269642271436965
2215	19	-0.8835066365247144
2215	20	-1.3079350494667494
2215	21	-0.6022549304481117
2215	22	-0.3292356123824618
2215	23	-0.6267056197721743
2215	24	-1.6075905007473423
2215	25	-0.8342546611686075
2216	1	-0.704787855874585
2216	2	-0.6407867123618196
2216	3	-1.5407677387991647
2216	4	-0.776304854506634
2216	5	-0.6200814318148176
2216	6	-0.5982336502614061
2216	7	-0.44725975350576164
2216	8	-0.5143816034515609
2216	9	-0.3130360260594145
2216	10	-0.45637319358623785
2216	11	-0.49888236492013505
2216	12	-0.3094240057672337
2216	13	-1.2301657074910537
2216	14	-0.8711266546843675
2216	15	-1.2943051413432158
2216	16	-0.9792351650652833
2216	17	-1.9261885312157148
2216	18	-0.9854883562713042
2216	19	-0.7410161772980217
2216	20	-0.7492931759702941
2216	21	-0.5479905818520692
2216	22	-0.6175512983144174
2216	23	-0.6082336843488605
2216	24	-0.5967138292809011
2216	25	-0.5804444826799156
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	4694.0
3	492.0
4	627.0
5	550.0
6	337.0
7	303.0
8	662.0
9	1379.0
10	2361.0
11	4176.0
12	6826.0
13	10103.0
14	13547.0
15	17556.0
16	19776.0
17	21275.0
18	21629.0
19	22090.0
20	22588.0
21	24033.0
22	25476.0
23	26452.0
24	27604.0
25	28937.0
26	30546.0
27	33937.0
28	37909.0
29	44522.0
30	53314.0
31	68794.0
32	89589.0
33	126497.0
34	181897.0
35	286565.0
36	520965.0
37	1783228.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.576080826882354	21.041484395113834	13.696295265549818	22.686139512453995
2	14.6256921793752	21.36555369427226	40.4670742736424	23.541679852710136
3	18.36941518200546	26.963784601689138	30.062595766063797	24.6042044502416
4	12.179016895188036	18.86308050914353	38.02637233125566	30.93153026441278
5	12.166059596124507	39.83341684071858	35.08084265519707	12.91968090795985
6	30.30701539663166	38.57446807313358	17.76124501076768	13.357271519467078
7	25.183418314737583	34.16342482514876	23.038612027604817	17.61454483250884
8	24.044083070280823	37.98464206707072	21.022025494495104	16.949249368153353
9	23.90012292147884	18.13122768389819	22.1860324278568	35.78261696676617
10	14.636470635252557	31.673748669485956	33.5589836364363	20.130797058825184
11	30.9683224612725	26.885025143047507	22.818919411693205	19.32773298398678
12	20.54880824886616	29.322795641679466	31.150111738100073	18.978284371354302
13	26.3464036616281	24.617154538536703	26.295269421442946	22.741172378392253
14	19.887651799932073	25.297057521681886	28.13646228951596	26.678828388870084
15	23.542079682893576	30.868131738272403	23.898059107440396	21.691729471393625
16	20.337490816667337	32.18025471631486	27.119017960924513	20.363236506093287
17	20.21633071215753	31.380980826408983	28.535991669112263	19.86669679232122
18	20.21278092456435	30.367597196311646	29.69332196550822	19.726299913615787
19	20.619413024460645	30.29348214207832	29.995078663996562	19.092026169464475
20	21.7033883020674	29.410584620890745	29.101869090771988	19.784157986269868
21	21.20917206312335	29.913054585806474	28.956829471968813	19.92094387910136
22	20.58979276471654	29.665551312482624	28.639025916509382	21.105630006291452
23	19.89467363514961	30.244440905302504	29.09336938005676	20.76751607949113
24	20.49236013276545	30.3227335229393	28.76582949984437	20.419076844450874
25	20.97095771741048	29.12462294673232	27.742171686117207	22.162247649739992
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	9565.0
1	9565.0
2	9130.0
3	8695.0
4	8695.0
5	8695.0
6	11200.0
7	13705.0
8	13705.0
9	13705.0
10	18098.5
11	22492.0
12	22492.0
13	22492.0
14	33034.0
15	43576.0
16	43576.0
17	43576.0
18	65487.5
19	87399.0
20	87399.0
21	87399.0
22	119326.0
23	151253.0
24	151253.0
25	151253.0
26	190373.5
27	229494.0
28	229494.0
29	229494.0
30	267107.0
31	304720.0
32	304720.0
33	304720.0
34	333363.5
35	362007.0
36	362007.0
37	362007.0
38	377172.5
39	392338.0
40	392338.0
41	392338.0
42	392416.0
43	392494.0
44	392494.0
45	392494.0
46	434780.5
47	477067.0
48	477067.0
49	477067.0
50	429502.0
51	381937.0
52	381937.0
53	381937.0
54	325749.5
55	269562.0
56	269562.0
57	269562.0
58	228232.0
59	186902.0
60	186902.0
61	186902.0
62	153522.5
63	120143.0
64	120143.0
65	120143.0
66	91444.0
67	62745.0
68	62745.0
69	62745.0
70	45395.0
71	28045.0
72	28045.0
73	28045.0
74	19555.5
75	11066.0
76	11066.0
77	11066.0
78	7485.0
79	3904.0
80	3904.0
81	3904.0
82	2650.0
83	1396.0
84	1396.0
85	1396.0
86	932.5
87	469.0
88	469.0
89	469.0
90	327.0
91	185.0
92	185.0
93	185.0
94	121.0
95	57.0
96	57.0
97	57.0
98	38.5
99	20.0
100	20.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3125319411575082
2	0.17805615803052646
3	0.19661712955838928
4	0.21731219160987927
5	0.24306167858574945
6	0.1988635406358916
7	0.19456727945016844
8	0.21798611493312994
9	0.21756491285609825
10	0.22739296132017087
11	0.20139075309808166
12	0.20751222328427546
13	0.22026060614910103
14	0.20956207339249633
15	0.19931282285139204
16	0.20338444292936497
17	0.2031036415446772
18	0.20627669719164918
19	0.20563085400686726
20	0.2009133907441124
21	0.19802113648182823
22	0.20240163808295772
23	0.20582741497614873
24	0.2218892541802902
25	0.21602050524031544
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3561236.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.07476278711812
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.36569914252904	68.87840062715391
2	8.355474595757869	12.879924448836592
3	1.2879814287927631	2.9781258929524705
4	0.3496279176864578	1.0778995527775117
5	0.1690227118802272	0.651369271190196
6	0.09277751368933917	0.42904829157506524
7	0.07178862140721119	0.3873163676042523
8	0.05044457594698668	0.3110402980008611
9	0.0358367224292819	0.24858961922742281
>10	0.192921145315822	2.670487946976163
>50	0.015766029479635072	0.8303536774133832
>100	0.009607366566741021	1.392335346824914
>500	0.0011032151390277043	0.5509096608483294
>1k	0.0014709534940096638	2.5935297067374923
>5k	3.309645361521743E-4	1.8739204467668
>10k+	1.4709534940096636E-4	2.2467488451146864
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	29105	0.8172724301338076	No Hit
CCCATGTACTCTGCGTTGATACCAC	25530	0.7168859351079232	No Hit
GAGTACATGGGAAGCAGTGGTATCA	14520	0.40772361056666845	No Hit
CATGTACTCTGCGTTGATACCACTG	10114	0.28400252047322894	No Hit
GCTTCCCATGTACTCTGCGTTGATA	9117	0.2560066224198565	No Hit
GCGTTGATACCACTGCTTCCCATGT	8374	0.23514307953755384	No Hit
GTATCAACGCAGAGTACATGGGAAG	7785	0.21860387797944308	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	7570	0.21256664820865565	No Hit
GGTATCAACGCAGAGTACATGGGAA	7220	0.20273859974458303	No Hit
TATCAACGCAGAGTACATGGGAAGC	6926	0.19448303903476208	No Hit
GTATCAACGCAGAGTACTTTTTTTT	6721	0.18872661064866242	No Hit
GGTATCAACGCAGAGTACTTTTTTT	6280	0.17634326958393096	No Hit
ACTCTGCGTTGATACCACTGCTTCC	6122	0.1719066077058639	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4638	0.13023568221819615	No Hit
GTGGTATCAACGCAGAGTACATGGG	4460	0.12523741757075352	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	4194	0.11776810073805836	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	5.6160276937557634E-5	0.0	0.0	5.6160276937557634E-5	0.0
11	5.6160276937557634E-5	0.0	0.0	5.6160276937557634E-5	0.0
12	5.6160276937557634E-5	0.0	0.0	5.6160276937557634E-5	0.0
13	5.6160276937557634E-5	0.0	0.0	8.424041540633645E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	255	0.0	11.554822	4
AGACCGT	75	2.0780468E-4	11.398345	6
GTACTAG	190	9.094947E-12	10.512677	1
TACACCG	120	1.5160513E-6	10.296548	5
TAGACAG	480	0.0	10.098537	5
CTAGGAC	275	0.0	10.022652	3
TATTCCG	105	4.0869007E-5	9.957101	5
ACTAGAC	210	6.548362E-11	9.504238	3
GTCTAGG	395	0.0	9.391071	1
CGGTGAA	155	4.2712418E-7	9.192601	9
GTGTTAG	445	0.0	9.190858	1
TTACACC	395	0.0	9.143833	4
ACACCGT	105	4.5015945E-4	9.046306	6
TAGACTG	515	0.0	9.043122	5
CCTGGAC	550	0.0	8.985826	3
CCTATAC	350	0.0	8.961139	3
CGCAAAA	170	1.6753074E-7	8.945165	2
CTTACAC	565	0.0	8.91548	3
GACTCCG	140	1.0873726E-5	8.82052	7
GTATTAG	490	0.0	8.735023	1
>>END_MODULE