FastQCFastQC Report
Wed 25 May 2016
SRR1294990_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294990_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3561236
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC289080.8117406428554581No Hit
GTACATGGGAAGCAGTGGTATCAAC288450.809971594131925No Hit
GTATCAACGCAGAGTACTTTTTTTT156560.4396226478672011No Hit
GAGTACATGGGAAGCAGTGGTATCA155930.4378535991436681No Hit
GGTATCAACGCAGAGTACTTTTTTT151320.4249086553095611No Hit
CATGTACTCTGCGTTGATACCACTG112750.31660356123548117No Hit
TATCAACGCAGAGTACTTTTTTTTT112270.3152557145889798No Hit
GCTTCCCATGTACTCTGCGTTGATA100430.28200883064194565No Hit
ACGCAGAGTACATGGGAAGCAGTGG95950.26942892860793277No Hit
GCGTTGATACCACTGCTTCCCATGT91960.25822495335889No Hit
GTATCAACGCAGAGTACATGGGAAG81380.228516166858922No Hit
GGTATCAACGCAGAGTACATGGGAA79890.22433222622707397No Hit
TATCAACGCAGAGTACATGGGAAGC77720.21823883617934897No Hit
ACTCTGCGTTGATACCACTGCTTCC69010.1937810355730426No Hit
ACGCAGAGTACTTTTTTTTTTTTTT63040.17701719290718168No Hit
GTGGTATCAACGCAGAGTACATGGG48840.13714339628151576No Hit
ACATGGGAAGCAGTGGTATCAACGC43390.1218397208160313No Hit
CAGTGGTATCAACGCAGAGTACATG40780.11451080467568002No Hit
CATGGGAAGCAGTGGTATCAACGCA38430.107911972135517No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCC907.4739837E-611.6102798
GGGACGC752.0766322E-411.3991837
GTGTAGG3650.010.9482471
GGACCGT700.001493289310.8562126
CGCGATC909.531711E-510.554502517
GACCGTG750.00265058410.13260657
AGTACCG750.002650892810.1324645
GTCCTAA2550.09.7011081
GTATAGA6050.09.4359161
CCTATAC3250.09.35304453
GCTACAC2950.09.3381823
GACTCCG1451.7095645E-69.1717577
ACACCGT1151.11239846E-49.0861786
TAAGGGT3350.09.073854
GTCCTAG3050.09.0466191
GTGTTAG5050.09.0435311
CCATGCG950.00182608658.99922859
TAGGACC1951.6532795E-88.7684794
CTACACT6100.08.7205644
CTTGGAC3500.08.684973
Produced by FastQC (version 0.11.5)