FastQCFastQC Report
Wed 25 May 2016
SRR1294989_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294989_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2574287
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC202990.7885290179377823No Hit
CCCATGTACTCTGCGTTGATACCAC175000.6797998824528889No Hit
GAGTACATGGGAAGCAGTGGTATCA99760.3875247787057154No Hit
CATGTACTCTGCGTTGATACCACTG68480.2660154054307076No Hit
GCTTCCCATGTACTCTGCGTTGATA62320.2420864495683659No Hit
GTATCAACGCAGAGTACTTTTTTTT56430.2192063278103801No Hit
GCGTTGATACCACTGCTTCCCATGT54900.21326293455236345No Hit
GGTATCAACGCAGAGTACATGGGAA54860.21310755172208848No Hit
GTATCAACGCAGAGTACATGGGAAG54630.21221410044800754No Hit
ACGCAGAGTACATGGGAAGCAGTGG52930.20561033016132232No Hit
GGTATCAACGCAGAGTACTTTTTTT50940.19788003435514379No Hit
TATCAACGCAGAGTACATGGGAAGC49120.19081011557763372No Hit
ACTCTGCGTTGATACCACTGCTTCC40570.157597035606364No Hit
TATCAACGCAGAGTACTTTTTTTTT37870.14710869456280515No Hit
GTGGTATCAACGCAGAGTACATGGG34200.13285231988507887No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN30960.12026631063280822No Hit
ATTCCATTCCATTCCATTCCATTCC30680.1191786308208836No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA26330.10228074802848322No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGA907.3613646E-611.6258071
TAGACTG3750.010.1400285
CTTACAC3300.09.7937733
GCTCTAA1752.5193003E-99.7837951
GTCTTAG2750.09.684971
GTGTTAG4250.09.6239291
GCCCTAC1002.7216136E-49.5120241
GCCTAAG2002.582965E-109.5120241
GTAGGAC1401.0505009E-69.505723
CTAATAC2950.09.3446063
TTACACT4850.09.2124474
GCCCTAG1554.1853127E-79.2051851
GTCCTGG4450.09.1913941
CGCAGAA1451.6932172E-69.1779372
GTTAGCC2600.09.1401163
CTTAGAC2304.1836756E-119.0924283
TTAGACT3150.09.0537734
TACGGAA1054.4670264E-49.0530672
AGCGCAG850.00744239438.9404617
CTACCCT2356.548362E-118.8996674
Produced by FastQC (version 0.11.5)