##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294989_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2574287
Sequences flagged as poor quality	0
Sequence length	25
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.204996567981738	33.0	33.0	33.0	27.0	33.0
2	31.104894675690783	33.0	33.0	33.0	27.0	33.0
3	31.207682360203037	33.0	33.0	33.0	27.0	33.0
4	34.70869681585619	37.0	37.0	37.0	27.0	37.0
5	34.95859746795909	37.0	37.0	37.0	33.0	37.0
6	34.91948605575058	37.0	37.0	37.0	33.0	37.0
7	34.887138458143944	37.0	37.0	37.0	33.0	37.0
8	34.81853965777709	37.0	37.0	37.0	33.0	37.0
9	34.706401034538885	37.0	37.0	37.0	27.0	37.0
10	34.671627910951656	37.0	37.0	37.0	27.0	37.0
11	34.80663539069265	37.0	37.0	37.0	33.0	37.0
12	34.73607604746479	37.0	37.0	37.0	27.0	37.0
13	34.63558725192645	37.0	37.0	37.0	27.0	37.0
14	36.06976261776562	40.0	37.0	40.0	27.0	40.0
15	36.21037087162387	40.0	37.0	40.0	27.0	40.0
16	36.29044663629191	40.0	37.0	40.0	27.0	40.0
17	36.22257968905565	40.0	37.0	40.0	27.0	40.0
18	36.162102360770184	40.0	37.0	40.0	27.0	40.0
19	36.26489742596688	40.0	37.0	40.0	27.0	40.0
20	36.27725929548648	40.0	37.0	40.0	27.0	40.0
21	36.28976489412408	40.0	37.0	40.0	27.0	40.0
22	36.19662182188699	40.0	37.0	40.0	27.0	40.0
23	36.11140288553685	40.0	37.0	40.0	27.0	40.0
24	36.183134592219126	40.0	37.0	40.0	27.0	40.0
25	35.077837474997935	37.0	33.0	40.0	27.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.4749189720728815
1101	2	0.4463978978743448
1101	3	0.4168788241130521
1101	4	0.6005466499863985
1101	5	0.28782666017986713
1101	6	0.3693564840067509
1101	7	0.3260296463914045
1101	8	0.45515458887292937
1101	9	0.38283879854686376
1101	10	0.5438135682888685
1101	11	0.38710556838955057
1101	12	0.49941255359652814
1101	13	0.42517575145524233
1101	14	1.0072891798017594
1101	15	0.797404784480392
1101	16	0.7495667150905163
1101	17	0.8165955505447613
1101	18	0.8232262254254863
1101	19	0.6541593837482438
1101	20	0.6056976017292826
1101	21	0.7176208010417682
1101	22	0.7358273168340617
1101	23	0.8342631097066828
1101	24	0.7156645129939605
1101	25	1.0136728646970212
1102	1	0.36836051363503586
1102	2	0.33115701190821767
1102	3	0.34900627036059007
1102	4	0.4411898902917102
1102	5	0.24408631941847148
1102	6	0.06986239348588441
1102	7	0.12864588640301378
1102	8	0.2388399702968087
1102	9	0.21082633479538515
1102	10	0.48522509151999316
1102	11	0.3503429704468104
1102	12	0.3971818391588968
1102	13	0.3209887141618921
1102	14	0.676969112554211
1102	15	0.5234376182959153
1102	16	0.5933767340292064
1102	17	0.5501881965262783
1102	18	0.5819984851387332
1102	19	0.5519481819009613
1102	20	0.5297094308151955
1102	21	0.7074868798550824
1102	22	0.7040729729195903
1102	23	0.6366707202920949
1102	24	0.6625565759608918
1102	25	0.8070914502705335
1103	1	0.38976558150036666
1103	2	0.28583360699987637
1103	3	0.3906796810361861
1103	4	0.2841592434938889
1103	5	0.21158327270346433
1103	6	0.14034187902700523
1103	7	0.15061321970094355
1103	8	0.36409069250269965
1103	9	0.3375794078478407
1103	10	0.40862458910604715
1103	11	0.2959077070353189
1103	12	0.40707379263945853
1103	13	0.3538422605421587
1103	14	0.6631728193613782
1103	15	0.5687912754567321
1103	16	0.48570323686149663
1103	17	0.35691201951541984
1103	18	0.6214966655723657
1103	19	0.5899477430433961
1103	20	0.495097855410485
1103	21	0.5335009117027099
1103	22	0.6737156371781623
1103	23	0.6569610526250784
1103	24	0.5770349241772408
1103	25	0.5205218577956927
1104	1	0.2976258442561388
1104	2	0.1703248025918036
1104	3	0.28083012948119546
1104	4	0.22178988313928016
1104	5	0.20743464972625958
1104	6	0.21407527650788438
1104	7	0.1858367079130403
1104	8	0.2744964379889083
1104	9	0.27783106500730526
1104	10	0.47185378269458766
1104	11	0.30193983063654173
1104	12	0.4324166337053299
1104	13	0.4167180592501225
1104	14	0.7046796435474576
1104	15	0.6604076645571979
1104	16	0.5708955505261315
1104	17	0.6402589815695876
1104	18	0.6966984513515087
1104	19	0.48300081951986584
1104	20	0.5358840067000799
1104	21	0.46473912419705243
1104	22	0.49825991503667666
1104	23	0.5311611603213677
1104	24	0.6483379176180861
1104	25	0.7759127910950596
1105	1	0.24307194874960558
1105	2	0.1652619093442631
1105	3	0.2044489689874318
1105	4	0.30675507570701654
1105	5	0.1509938908952364
1105	6	0.1876636710430759
1105	7	0.20254565926171608
1105	8	0.33074767358026236
1105	9	0.41133153815641776
1105	10	0.43029573823702094
1105	11	0.33174888399563685
1105	12	0.45197311242152693
1105	13	0.4110840921517749
1105	14	0.6817742839145922
1105	15	0.5186564724586589
1105	16	0.6240064730219359
1105	17	0.4163481471137871
1105	18	0.534406393139669
1105	19	0.44750916618659886
1105	20	0.4583028398800906
1105	21	0.3966395230129933
1105	22	0.5043470640957821
1105	23	0.7231126170671018
1105	24	0.5697321654955019
1105	25	0.6002660661040906
1106	1	-0.03124329262870873
1106	2	-0.1372340881171894
1106	3	-0.19938120798967773
1106	4	-0.15190483111676656
1106	5	-0.44962992817559666
1106	6	-0.45822705604869896
1106	7	-0.6946860985378223
1106	8	-0.20805495439279298
1106	9	0.036184778409698026
1106	10	-0.39692260564955006
1106	11	-0.3638735648367728
1106	12	-0.8779893141986008
1106	13	-0.28308121629610383
1106	14	-0.4233266089039205
1106	15	-0.11560922398304996
1106	16	-0.18715880657882167
1106	17	-0.3377149703949769
1106	18	-0.5316336899400511
1106	19	-0.6087510204542994
1106	20	-0.1938981957316841
1106	21	-0.07692100546632474
1106	22	0.15089298150646613
1106	23	-0.5894257856676077
1106	24	0.04021434518424627
1106	25	0.0067043837397804396
1107	1	-1.0000792353898866
1107	2	-0.9617328572198502
1107	3	-0.7544651912349423
1107	4	-0.9160932631966645
1107	5	-0.9433749857245886
1107	6	-0.8463200453827895
1107	7	-0.953474579797728
1107	8	-0.8551529846776162
1107	9	-0.8773648724401966
1107	10	-0.894081831511528
1107	11	-0.8647557312160927
1107	12	-0.8440222061227445
1107	13	-0.6678286378386247
1107	14	-1.1120209355988848
1107	15	-0.9821907803626218
1107	16	-0.9765753249137461
1107	17	-0.968149989913293
1107	18	-1.080733570063785
1107	19	-1.133015416085101
1107	20	-1.223297640780011
1107	21	-1.1671948906030636
1107	22	-1.0903202138247892
1107	23	-0.8111331702133455
1107	24	-0.8074751724884237
1107	25	-0.8008096088105674
1108	1	0.14503923605481717
1108	2	-0.04150234789333496
1108	3	-0.035395088333771696
1108	4	-0.010862833901470026
1108	5	-0.3006704143114405
1108	6	0.16412774540625463
1108	7	0.07253076295015859
1108	8	0.29432958294705713
1108	9	-0.09003730709581248
1108	10	-0.3579300099388547
1108	11	0.042218783704491614
1108	12	0.15642579011984736
1108	13	0.09805160758758547
1108	14	-0.25930001390559454
1108	15	0.06708378943317683
1108	16	-0.7188180797496102
1108	17	-0.6381839446958324
1108	18	-0.5114696838525603
1108	19	0.010934836921393298
1108	20	-0.8116479825615528
1108	21	-0.0843843887732163
1108	22	-0.22055166441523966
1108	23	0.09781638640117052
1108	24	-0.2610279779924767
1108	25	-1.0426293921239633
1109	1	0.1792913403730374
1109	2	0.11839841748159685
1109	3	0.25434510508750563
1109	4	0.22600962321711648
1109	5	0.19251915724123592
1109	6	0.0733727312384147
1109	7	0.07714746733601174
1109	8	0.3064534485884778
1109	9	0.33081432631258423
1109	10	0.33750940160900456
1109	11	0.1605464773220433
1109	12	0.23041263914161902
1109	13	0.12388889973657768
1109	14	0.5224216004428399
1109	15	0.4324353114065076
1109	16	0.27399148768314063
1109	17	0.38335196563491536
1109	18	0.3265828249128475
1109	19	0.3609510307448147
1109	20	0.3178466529707933
1109	21	0.47457888077613575
1109	22	0.28698461525654295
1109	23	0.29106904590271654
1109	24	0.4210603174822296
1109	25	0.39563241770805035
1110	1	-0.10746007575206562
1110	2	-0.11850504698103848
1110	3	-0.040709081441558936
1110	4	-1.145221375091971
1110	5	-0.35200470940179684
1110	6	-0.08632429181671597
1110	7	-0.07103414593992596
1110	8	0.11885889883169654
1110	9	0.13248933825685327
1110	10	-0.10617423225778566
1110	11	-0.12822312194509067
1110	12	0.09275010783776594
1110	13	0.1236834031376901
1110	14	0.13865351107096302
1110	15	0.0871272855227545
1110	16	0.12168833900762621
1110	17	0.09709711794288012
1110	18	0.1700440211850136
1110	19	0.2202557131025955
1110	20	0.03890451372335235
1110	21	0.21637182409719458
1110	22	-0.41189910656418505
1110	23	-0.03581518298754105
1110	24	0.12951413542860024
1110	25	0.11277226707823473
1111	1	-0.008121416939896875
1111	2	-0.13997967224262808
1111	3	-0.04913439250805851
1111	4	0.03533165185785947
1111	5	-0.009091369719513409
1111	6	-0.028972346281392447
1111	7	0.04489261437522174
1111	8	0.1897386050021126
1111	9	0.20363588472540073
1111	10	0.10163820429708892
1111	11	-0.043121743654182865
1111	12	0.11389183067691278
1111	13	-0.0010532338970179467
1111	14	0.034157859680853164
1111	15	0.11382692373614844
1111	16	-0.023926693898168594
1111	17	0.08950072238913975
1111	18	0.09140008595584703
1111	19	-0.001640108597626977
1111	20	0.09839895704627821
1111	21	-0.05771872017810864
1111	22	0.04456632788355819
1111	23	0.16311434565891147
1111	24	0.19362037161826606
1111	25	0.08338447060140908
1112	1	-0.10239704524313709
1112	2	-0.49234188086543185
1112	3	-0.2441312768970505
1112	4	-0.3248982754585299
1112	5	-0.22973947738626777
1112	6	-0.28669931204904486
1112	7	-0.26132966096790256
1112	8	-0.16004502632668505
1112	9	-0.2356123799043175
1112	10	0.08141818589349015
1112	11	-0.2239959227119428
1112	12	-0.042376768192795566
1112	13	-0.1132465669670708
1112	14	-0.36129186877928987
1112	15	-0.2595841525085447
1112	16	-0.2278455023216992
1112	17	-0.42593451198530374
1112	18	-0.2757829135836545
1112	19	-0.19066986108100537
1112	20	-0.8653173092856292
1112	21	-1.2074681840472152
1112	22	-1.621960080953336
1112	23	-0.54063828519471
1112	24	-0.3335322162027694
1112	25	-1.534879323855165
1113	1	-0.14185591970201017
1113	2	-0.21518218870573946
1113	3	-0.11835927112504407
1113	4	-0.5785875491478052
1113	5	-0.20247637012316488
1113	6	-0.08057858092831793
1113	7	-0.07219546183370795
1113	8	-0.12692333320499216
1113	9	-0.15746423175616542
1113	10	-0.08003924556394537
1113	11	-0.13075157446759533
1113	12	-0.19578225159828122
1113	13	-0.15921818793869136
1113	14	-0.4708493483367704
1113	15	-0.36893720686159526
1113	16	-0.369718999195193
1113	17	-0.40608843213922086
1113	18	-0.7797824325831755
1113	19	-0.9443903998015415
1113	20	-1.273566467534792
1113	21	-0.9377904543694839
1113	22	-0.3680904320836902
1113	23	-1.8145247690811956
1113	24	-0.9144004220709689
1113	25	-0.49104575002159123
1114	1	-0.3649629142044901
1114	2	-0.4240723278792089
1114	3	-0.4339243064502476
1114	4	-0.6898900471044271
1114	5	-0.6437946552552276
1114	6	-0.46738873174622597
1114	7	-0.4189240455785139
1114	8	-0.21132224489758045
1114	9	-1.1873489069994108
1114	10	-0.4086813245446024
1114	11	-0.4970105177345303
1114	12	-0.5000021300034376
1114	13	-0.4788578260652798
1114	14	-0.975723330847714
1114	15	-1.3489601038642718
1114	16	-1.136495560278128
1114	17	-1.5759709800325865
1114	18	-1.819280545959728
1114	19	-1.6489821725557903
1114	20	-1.0096659155434793
1114	21	-0.7749700362892469
1114	22	-0.5121496955727665
1114	23	-0.3901602308840708
1114	24	-0.9123304546530164
1114	25	-0.6994209025749711
1115	1	-0.7203988129369918
1115	2	-0.3510608063773475
1115	3	-0.4196658060554519
1115	4	-0.5636796968548268
1115	5	-0.4747580691599538
1115	6	-0.3371648298286374
1115	7	-0.319498477781913
1115	8	-0.1897696484245941
1115	9	-0.5073171563461827
1115	10	-0.13871985281394927
1115	11	-0.34743053501438936
1115	12	-0.5264251425775015
1115	13	-0.4068626128332369
1115	14	-0.45862246421504693
1115	15	-1.4610013380564695
1115	16	-0.7892787880562651
1115	17	-0.6337201120020737
1115	18	-2.1477859741229537
1115	19	-1.1457920149241403
1115	20	-0.9313736453561816
1115	21	-1.020852871188417
1115	22	-0.7514509472987783
1115	23	-0.41204140529513467
1115	24	-0.4406659022090267
1115	25	-0.6869147807224536
1116	1	-1.5267817322860502
1116	2	-0.8614099904398707
1116	3	-1.6446860666923442
1116	4	-0.9756682743297915
1116	5	-0.997435798880062
1116	6	-0.5122832686456604
1116	7	-1.3402707620609036
1116	8	-3.006149929393615
1116	9	-1.5439023084474073
1116	10	-0.8747529285054725
1116	11	-0.6249169660380502
1116	12	-0.8814993193406622
1116	13	-1.8736441628077074
1116	14	-1.8172600684183777
1116	15	-1.6461577289555365
1116	16	-1.2878710370307758
1116	17	-1.8728538608033887
1116	18	-2.628266459105177
1116	19	-1.5403979576445437
1116	20	-1.1240788243762623
1116	21	-0.9511409435427609
1116	22	-0.7048565562548745
1116	23	-0.5944464086230923
1116	24	-0.7659966106924756
1116	25	-0.6324302726719395
1201	1	0.21268899865646773
1201	2	0.2344921764770973
1201	3	0.26680618488786934
1201	4	0.41561124640692526
1201	5	0.23636761075471924
1201	6	0.025479344197407272
1201	7	0.11542296493726667
1201	8	0.20383472229882216
1201	9	0.1479861935247655
1201	10	0.46299235560325513
1201	11	0.21992732290621575
1201	12	0.16971039135199106
1201	13	0.2791424627100909
1201	14	0.7012379429030631
1201	15	0.5322787241000952
1201	16	0.4595238765731011
1201	17	0.42537118169327215
1201	18	0.38410873274867186
1201	19	0.3696659111191778
1201	20	0.4211780534809151
1201	21	0.48478482346003204
1201	22	0.48764092695275707
1201	23	0.5263909818345525
1201	24	0.5144829826598922
1201	25	0.6521100207612989
1202	1	0.29413975129365966
1202	2	0.3103203522315816
1202	3	0.36621441605733906
1202	4	0.5624702336491083
1202	5	0.35802428863836866
1202	6	-0.02418388866406218
1202	7	0.1076332601689316
1202	8	0.2560392114966845
1202	9	0.25580698977592675
1202	10	0.5111879330545435
1202	11	0.30160585071591584
1202	12	0.22066984702772885
1202	13	0.3009408559160036
1202	14	0.5521489577050147
1202	15	0.3973746696676699
1202	16	0.35128325941575866
1202	17	0.49074574817321803
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2210	22	0.4549188829408024
2210	23	0.5258646953048967
2210	24	0.512032967473715
2210	25	0.614919880750108
2211	1	0.07862459977851088
2211	2	-0.09564539216894374
2211	3	0.007995707229934368
2211	4	0.006970892410642193
2211	5	0.04709938745259734
2211	6	0.018914925565191254
2211	7	0.025440901802660676
2211	8	0.013751991470329017
2211	9	0.09179117949924631
2211	10	0.17510361157891197
2211	11	0.01894972423370689
2211	12	0.12878051896448994
2211	13	-0.008434638155144114
2211	14	-0.16427791976533967
2211	15	-0.08997183889622562
2211	16	-0.1014809039570963
2211	17	-0.11019232824312297
2211	18	0.04365912585838316
2211	19	0.01632388591274747
2211	20	-0.0052288051971274285
2211	21	0.10032209974306738
2211	22	0.045299178305917565
2211	23	0.25454016598374096
2211	24	0.22113637446582146
2211	25	0.1953005703247257
2212	1	0.09950836489812076
2212	2	0.08378955628612417
2212	3	0.1630824473499004
2212	4	0.06196239848684115
2212	5	0.14924411030111173
2212	6	0.16238384971544662
2212	7	0.12599670787460582
2212	8	0.22717386580563925
2212	9	0.04828459280875563
2212	10	0.27539249006900945
2212	11	0.1257191120756218
2212	12	0.21524321801867075
2212	13	0.1443610397280395
2212	14	0.18848639157454272
2212	15	0.0943565739169756
2212	16	-0.035267832673369526
2212	17	0.10754037549052242
2212	18	0.16258994079294098
2212	19	0.14648142763790162
2212	20	-0.04635096738994804
2212	21	0.20332682174077377
2212	22	-0.41380616620138966
2212	23	0.058524454351740474
2212	24	0.17314720811820905
2212	25	0.29496512414557685
2213	1	-0.11028387498763692
2213	2	-0.6073954621851883
2213	3	-0.18751647559648177
2213	4	-0.07391982623608584
2213	5	0.015077542384538845
2213	6	0.06694584473844145
2213	7	0.03307564106216887
2213	8	0.1703885231456823
2213	9	-0.10531037206071403
2213	10	-1.4927423526847932
2213	11	-0.40691080340870656
2213	12	-0.04760031338861381
2213	13	-0.04055125721076536
2213	14	0.08833822317981799
2213	15	-0.03362517447008173
2213	16	-0.15289433587330592
2213	17	7.780408825155405E-4
2213	18	-0.05997695771300471
2213	19	0.02751920322636181
2213	20	-0.061811340345279575
2213	21	0.011206993038314295
2213	22	0.16543170505212856
2213	23	-0.739448631417261
2213	24	-1.3687178237081525
2213	25	-1.2489935539120935
2214	1	0.03302103223751374
2214	2	-0.07285779428980632
2214	3	-0.022429237466155882
2214	4	0.03323389321825232
2214	5	0.05925402771384114
2214	6	0.024937889854669493
2214	7	0.11721743890016967
2214	8	0.09296741336014236
2214	9	-0.16468102809231056
2214	10	0.03591782556114964
2214	11	-0.03862196016532948
2214	12	-0.0390250921875861
2214	13	-0.013487624566181466
2214	14	0.09231844948919843
2214	15	-0.1399042101171304
2214	16	-0.0959158209134543
2214	17	-0.07060975863042529
2214	18	0.10491408565331994
2214	19	-0.6526757278377389
2214	20	-0.28245848320548106
2214	21	-0.21315435659317927
2214	22	-0.326133526494111
2214	23	-1.602034011480356
2214	24	-1.209835780455343
2214	25	-1.5500817815836925
2215	1	-0.33047796001536156
2215	2	-0.12287605368241472
2215	3	-1.0655201724541534
2215	4	-0.7070067023913538
2215	5	-0.16791691232895545
2215	6	-0.19899685396766387
2215	7	-0.17186890607276695
2215	8	-0.210618870569661
2215	9	-0.23770408740267612
2215	10	-1.24967396081788
2215	11	-0.5009496204142536
2215	12	-0.4667315518359345
2215	13	-0.4807083736617841
2215	14	-0.7064861760808085
2215	15	-0.8878303500708213
2215	16	-0.49531577764779655
2215	17	-0.4042589276941939
2215	18	-0.4765666065115255
2215	19	-0.9103273771802947
2215	20	-1.156312264559702
2215	21	-0.6750222121372857
2215	22	-0.3458899215652522
2215	23	-0.5495225685706799
2215	24	-1.7388656980149904
2215	25	-0.7009344041893613
2216	1	-0.5542327336952795
2216	2	-0.5027535069201647
2216	3	-1.490331902587826
2216	4	-0.7853060836067769
2216	5	-0.5699464357855817
2216	6	-0.5542046362171789
2216	7	-0.3576377175308849
2216	8	-0.48408936531888
2216	9	-0.28727662484782
2216	10	-0.26118530305993914
2216	11	-0.5086103726798115
2216	12	-0.34493470980056173
2216	13	-0.9666741830829864
2216	14	-0.854610866849157
2216	15	-1.0905415218204908
2216	16	-1.0136446737523883
2216	17	-1.805719839742821
2216	18	-0.8193037590743728
2216	19	-0.6529074775480481
2216	20	-0.5835013569018912
2216	21	-0.4400373229894683
2216	22	-0.5006410583596903
2216	23	-0.3320455989658697
2216	24	-0.3938450504437583
2216	25	-0.3174050724295796
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	3425.0
3	368.0
4	445.0
5	393.0
6	252.0
7	209.0
8	253.0
9	536.0
10	1069.0
11	2121.0
12	3728.0
13	5922.0
14	8316.0
15	11146.0
16	13015.0
17	14053.0
18	14176.0
19	14543.0
20	14735.0
21	15629.0
22	16597.0
23	16983.0
24	18145.0
25	19151.0
26	19987.0
27	22387.0
28	24783.0
29	29797.0
30	35565.0
31	45396.0
32	60751.0
33	87047.0
34	126368.0
35	203011.0
36	375081.0
37	1348904.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.40811173114693	21.505595909841944	13.67069863141815	22.41559372759298
2	14.495778533138534	21.932207453163784	40.60500816116531	22.967005852532374
3	18.05877228561397	27.267603309259435	30.45160165260561	24.222022752520985
4	11.83531290648994	18.61191995430766	38.60134561918455	30.951421520017846
5	12.118862576147652	39.472746510438796	35.22857244108256	13.179818472330993
6	29.80777352424288	38.89039179013703	17.842750685550026	13.459084000070062
7	24.518212994752915	34.2120248151494	23.75444538592586	17.51531680417182
8	23.139946967694364	38.603018037290795	21.445692886811337	16.8113421082035
9	23.37417475592085	18.209289451124604	22.59465536935196	35.821880423602586
10	14.382430377262843	31.68877042076172	33.90082006212568	20.027979139849755
11	30.552951874105688	27.38683198054349	22.78350587696749	19.276710268383333
12	19.858271983257968	29.842724254767866	31.63884892310375	18.66015483887041
13	25.842220256578763	25.10410469958358	26.57733803426656	22.476337009571093
14	19.38456459860607	25.463709802993407	28.55517336954152	26.596552228859004
15	23.281115892357725	31.186287618258003	24.060309879275145	21.472286610109126
16	20.217354240155455	32.52466148122255	27.171109535046124	20.08687474357587
17	19.567162825422937	31.7589352756692	28.987198535774382	19.686703363133482
18	19.527955803857818	30.797979845164026	30.184334846814526	19.48972950416363
19	20.06946060979284	30.297150028533192	30.42872190765211	19.204667454021855
20	21.33552561666727	29.61322470008887	29.429652111259934	19.621597571983926
21	21.10255511004493	30.303060970687252	28.978388042133258	19.615995877134562
22	20.11363180756396	30.00094589371191	29.286076016378367	20.59934628234577
23	19.552329965226374	30.63909430530799	29.41467966262616	20.393896066839478
24	20.011672109498246	30.313807661700377	29.304898236958614	20.36962199184276
25	20.809729901867083	29.25853529020395	28.036925103721437	21.89480970420753
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7445.0
1	7445.0
2	6791.0
3	6137.0
4	6137.0
5	6137.0
6	7942.5
7	9748.0
8	9748.0
9	9748.0
10	13404.0
11	17060.0
12	17060.0
13	17060.0
14	25580.5
15	34101.0
16	34101.0
17	34101.0
18	50146.0
19	66191.0
20	66191.0
21	66191.0
22	90609.5
23	115028.0
24	115028.0
25	115028.0
26	143906.0
27	172784.0
28	172784.0
29	172784.0
30	200878.0
31	228972.0
32	228972.0
33	228972.0
34	250502.5
35	272033.0
36	272033.0
37	272033.0
38	283720.0
39	295407.0
40	295407.0
41	295407.0
42	291367.5
43	287328.0
44	287328.0
45	287328.0
46	312055.5
47	336783.0
48	336783.0
49	336783.0
50	302020.5
51	267258.0
52	267258.0
53	267258.0
54	226906.5
55	186555.0
56	186555.0
57	186555.0
58	157125.5
59	127696.0
60	127696.0
61	127696.0
62	103200.0
63	78704.0
64	78704.0
65	78704.0
66	59270.5
67	39837.0
68	39837.0
69	39837.0
70	28215.0
71	16593.0
72	16593.0
73	16593.0
74	11312.5
75	6032.0
76	6032.0
77	6032.0
78	3959.0
79	1886.0
80	1886.0
81	1886.0
82	1215.0
83	544.0
84	544.0
85	544.0
86	331.5
87	119.0
88	119.0
89	119.0
90	71.0
91	23.0
92	23.0
93	23.0
94	15.5
95	8.0
96	8.0
97	8.0
98	11.5
99	15.0
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3156990654111216
2	0.18649824203750398
3	0.20405650185857288
4	0.22546048672894672
5	0.24915636834587596
6	0.20129845662119256
7	0.19617082322211937
8	0.2200220876693236
9	0.21959478488606746
10	0.23093773149613855
11	0.2049111074250851
12	0.21066027214525807
13	0.2221586015856041
14	0.21128180346635786
15	0.20285228492394206
16	0.20592109582187224
17	0.20526071879320373
18	0.2087956781819587
19	0.2077856897851716
20	0.2049499531326538
21	0.20199767935742985
22	0.20541610162347868
23	0.20922298096521486
24	0.22417857837917837
25	0.21862364219684907
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2574287.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.50651612949736
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.64128079070075	72.86078969313647
2	6.724089053288093	10.692177895428529
3	0.8577956369626758	2.046010279379564
4	0.24846208205579226	0.790174181381494
5	0.13022520569837656	0.5176876208662545
6	0.07272090990948223	0.34690717180019837
7	0.0511902782682202	0.28489724793639953
8	0.036397638322005085	0.23150795346592873
9	0.030059151493836454	0.21509085717753415
>10	0.17744521325288767	2.6503956695516986
>50	0.015713601616771287	0.8752275560998076
>100	0.011525876984548914	1.7230162785210155
>500	0.0010806405051620915	0.6179454131024478
>1k	0.0014736006888342596	2.503532244611176
>5k	4.420802066502779E-4	2.168452193776391
>10k+	9.8240045922284E-5	1.4761877437650393
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	20299	0.7885290179377823	No Hit
CCCATGTACTCTGCGTTGATACCAC	17500	0.6797998824528889	No Hit
GAGTACATGGGAAGCAGTGGTATCA	9976	0.3875247787057154	No Hit
CATGTACTCTGCGTTGATACCACTG	6848	0.2660154054307076	No Hit
GCTTCCCATGTACTCTGCGTTGATA	6232	0.2420864495683659	No Hit
GTATCAACGCAGAGTACTTTTTTTT	5643	0.2192063278103801	No Hit
GCGTTGATACCACTGCTTCCCATGT	5490	0.21326293455236345	No Hit
GGTATCAACGCAGAGTACATGGGAA	5486	0.21310755172208848	No Hit
GTATCAACGCAGAGTACATGGGAAG	5463	0.21221410044800754	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	5293	0.20561033016132232	No Hit
GGTATCAACGCAGAGTACTTTTTTT	5094	0.19788003435514379	No Hit
TATCAACGCAGAGTACATGGGAAGC	4912	0.19081011557763372	No Hit
ACTCTGCGTTGATACCACTGCTTCC	4057	0.157597035606364	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3787	0.14710869456280515	No Hit
GTGGTATCAACGCAGAGTACATGGG	3420	0.13285231988507887	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	3096	0.12026631063280822	No Hit
ATTCCATTCCATTCCATTCCATTCC	3068	0.1191786308208836	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	2633	0.10228074802848322	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	7.769141513747302E-5	0.0
8	0.0	0.0	0.0	7.769141513747302E-5	0.0
9	0.0	0.0	0.0	1.9422853784368255E-4	0.0
10	0.0	0.0	0.0	1.9422853784368255E-4	0.0
11	0.0	0.0	0.0	2.3307424541241906E-4	0.0
12	0.0	0.0	0.0	2.3307424541241906E-4	0.0
13	0.0	0.0	0.0	2.3307424541241906E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACGGA	90	7.3613646E-6	11.625807	1
TAGACTG	375	0.0	10.140028	5
CTTACAC	330	0.0	9.793773	3
GCTCTAA	175	2.5193003E-9	9.783795	1
GTCTTAG	275	0.0	9.68497	1
GTGTTAG	425	0.0	9.623929	1
GCCCTAC	100	2.7216136E-4	9.512024	1
GCCTAAG	200	2.582965E-10	9.512024	1
GTAGGAC	140	1.0505009E-6	9.50572	3
CTAATAC	295	0.0	9.344606	3
TTACACT	485	0.0	9.212447	4
GCCCTAG	155	4.1853127E-7	9.205185	1
GTCCTGG	445	0.0	9.191394	1
CGCAGAA	145	1.6932172E-6	9.177937	2
GTTAGCC	260	0.0	9.140116	3
CTTAGAC	230	4.1836756E-11	9.092428	3
TTAGACT	315	0.0	9.053773	4
TACGGAA	105	4.4670264E-4	9.053067	2
AGCGCAG	85	0.0074423943	8.940461	7
CTACCCT	235	6.548362E-11	8.899667	4
>>END_MODULE