FastQCFastQC Report
Wed 25 May 2016
SRR1294989_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294989_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2574287
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC205380.7978131420467103No Hit
CCCATGTACTCTGCGTTGATACCAC192090.7461871966878596No Hit
GTATCAACGCAGAGTACTTTTTTTT136120.5287677714256414No Hit
GGTATCAACGCAGAGTACTTTTTTT134560.5227078410449184No Hit
GAGTACATGGGAAGCAGTGGTATCA112010.43511077047741764No Hit
TATCAACGCAGAGTACTTTTTTTTT95910.37256918129175187No Hit
CATGTACTCTGCGTTGATACCACTG75210.29215856662446726No Hit
GCTTCCCATGTACTCTGCGTTGATA69400.2695892105270314No Hit
ACGCAGAGTACATGGGAAGCAGTGG68720.26694770241235727No Hit
GCGTTGATACCACTGCTTCCCATGT61500.2389011015477295No Hit
GGTATCAACGCAGAGTACATGGGAA58700.22802430342848332No Hit
GTATCAACGCAGAGTACATGGGAAG57160.2220420644628979No Hit
ACGCAGAGTACTTTTTTTTTTTTTT54420.2113983405890641No Hit
TATCAACGCAGAGTACATGGGAAGC53390.2073972327094842No Hit
ACTCTGCGTTGATACCACTGCTTCC46870.182069831374668No Hit
GTGGTATCAACGCAGAGTACATGGG35700.13867917602038934No Hit
ATTCCATTCCATTCCATTCCATTCC31450.12216975030367631No Hit
ACATGGGAAGCAGTGGTATCAACGC29740.11552713430942238No Hit
CAGTGGTATCAACGCAGAGTACATG28530.11082680369360527No Hit
GTACTTTTTTTTTTTTTTTTTTTTT27500.1068256958140254No Hit
CATGGGAAGCAGTGGTATCAACGCA26590.10329073642527037No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGTC500.00150059213.299327
TAAACCG701.09235385E-412.2131875
CTGCTCG658.031311E-411.69148359
CAGGACC1501.7644197E-1011.3989734
CTGTGCG600.005880522511.0825519
TAGGACA2250.010.55460554
GTCCTAG2550.010.447481
TACACTG5600.010.0080285
CTTGGAC2107.2759576E-129.9514873
TGGACAG3400.09.7785335
TAGACAG3650.09.6292715
GTCTTAT2700.09.5146691
CCTAGAC2106.730261E-119.49914553
ACTAGAC2201.6370905E-119.49914553
CTAGACA3350.09.35736754
CTAGACC1752.6751877E-89.227744
TCTGTCG2304.1836756E-119.0864928
GTGTTAC2201.6370905E-109.0821841
CTACACT3350.09.0738114
ATAGGAC2002.6611815E-99.0241883
Produced by FastQC (version 0.11.5)