FastQCFastQC Report
Wed 25 May 2016
SRR1294988_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294988_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4463916
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC670561.5021788044398685No Hit
CCCATGTACTCTGCGTTGATACCAC598211.340101381836038No Hit
GAGTACATGGGAAGCAGTGGTATCA320110.7171057878329252No Hit
CATGTACTCTGCGTTGATACCACTG224370.5026304258413464No Hit
GCGTTGATACCACTGCTTCCCATGT195850.4387403347195601No Hit
GCTTCCCATGTACTCTGCGTTGATA189400.42429113809489244No Hit
GTATCAACGCAGAGTACATGGGAAG185040.4145239292137217No Hit
ACGCAGAGTACATGGGAAGCAGTGG171100.38329574302025393No Hit
TATCAACGCAGAGTACATGGGAAGC153600.3440924963641789No Hit
GGTATCAACGCAGAGTACATGGGAA144650.3240428359315005No Hit
ACTCTGCGTTGATACCACTGCTTCC136660.30614375360109825No Hit
GTGGTATCAACGCAGAGTACATGGG93940.21044302804981097No Hit
CAGTGGTATCAACGCAGAGTACATG79080.17715387117499523No Hit
GTATCAACGCAGAGTACTTTTTTTT77490.1735919761931004No Hit
ACATGGGAAGCAGTGGTATCAACGC71730.1606885075794437No Hit
GCAGTGGTATCAACGCAGAGTACAT67690.15163815806569836No Hit
ATACCACTGCTTCCCATGTACTCTG66560.14910674842447752No Hit
CATGGGAAGCAGTGGTATCAACGCA65860.14753861855823452No Hit
GGGAAGCAGTGGTATCAACGCAGAG65240.14614970353384787No Hit
GTACTCTGCGTTGATACCACTGCTT63600.14247579927579282No Hit
GGTATCAACGCAGAGTACTTTTTTT62910.14093007126478185No Hit
CTGCTTCCCATGTACTCTGCGTTGA59010.13219334772428512No Hit
GTACATGGTAAGCAGTGGTATCAAC57850.12959473251736817No Hit
GATACCACTGCTTCCCATGTACTCT57210.1281610137825174No Hit
CCATGTACTCTGCGTTGATACCACT54720.12258295182973873No Hit
CTTCCCATGTACTCTGCGTTGATAC53820.12056678485885487No Hit
ATTCCATTCCATTCCATTCCATTCC53660.1202083551751422No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN51850.11615361937814242No Hit
TACCACTGCTTCCCATGTACTCTGC51760.11595200268105403No Hit
GTTGATACCACTGCTTCCCATGTAC51500.11536955444502092No Hit
TATCAACGCAGAGTACTTTTTTTTT49900.11178525760789405No Hit
GCAGAGTACATGGGAAGCAGTGGTA49030.10983629620270631No Hit
AAGCAGTGGTATCAACGCAGAGTAC47540.10649841977313193No Hit
ATGGGAAGCAGTGGTATCAACGCAG44980.10076354483372896No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCCG905.4217526E-712.6648929
GTACGGA1252.2122367E-710.6508311
TAGACAG6550.09.7239495
AGACAGT6650.09.7123796
GTCCTAG4500.09.5096691
GCACCGT1201.7060593E-59.4981366
ATTAGAC3850.09.3816313
CTAGGAC4500.09.0826263
TGCACCG1054.4661816E-49.0535715
ACCGAGT1054.5014295E-49.0464538
TAGACTG4750.09.0059215
TGTCGCC2850.08.99863710
TCTGTCG2750.08.980668
CTACACG850.00739860168.9471594
GTACTAG3000.08.8756921
GTGCTAG4000.08.7964451
GTACAAA8900.08.7617191
GTTCTAG4800.08.7171971
CGCAAAA2401.0186341E-108.7129842
TCTACAC5250.08.6903533
Produced by FastQC (version 0.11.5)