##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294988_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4463916 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.120172064169665 33.0 33.0 33.0 27.0 33.0 2 31.078538440239466 33.0 33.0 33.0 27.0 33.0 3 31.124494278118135 33.0 33.0 33.0 27.0 33.0 4 34.54600041757058 37.0 37.0 37.0 27.0 37.0 5 34.90667633530739 37.0 37.0 37.0 33.0 37.0 6 35.03723815591512 37.0 37.0 37.0 33.0 37.0 7 34.985434761765234 37.0 37.0 37.0 33.0 37.0 8 34.847439557554395 37.0 37.0 37.0 33.0 37.0 9 34.810382184611 37.0 37.0 37.0 33.0 37.0 10 34.736793658303604 37.0 37.0 37.0 27.0 37.0 11 34.92136411169027 37.0 37.0 37.0 33.0 37.0 12 34.83097240181043 37.0 37.0 37.0 33.0 37.0 13 34.73526204346139 37.0 37.0 37.0 27.0 37.0 14 36.206049800220256 40.0 37.0 40.0 27.0 40.0 15 36.324500953870995 40.0 37.0 40.0 27.0 40.0 16 36.40107363131385 40.0 37.0 40.0 33.0 40.0 17 36.35117439485868 40.0 37.0 40.0 27.0 40.0 18 36.28282521445296 40.0 37.0 40.0 27.0 40.0 19 36.35302165184112 40.0 37.0 40.0 27.0 40.0 20 36.36892293672193 40.0 37.0 40.0 27.0 40.0 21 36.369051523370956 40.0 37.0 40.0 27.0 40.0 22 36.295323433505466 40.0 37.0 40.0 27.0 40.0 23 36.21711878090896 40.0 37.0 40.0 27.0 40.0 24 36.28531204440227 40.0 37.0 40.0 27.0 40.0 25 35.234156735924245 37.0 33.0 40.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.46580672663731804 1101 2 0.4333518394131666 1101 3 0.4208271050695167 1101 4 0.4771829032612942 1101 5 0.3193432128038083 1101 6 0.3455900385366988 1101 7 0.3814065911907889 1101 8 0.4151179206818014 1101 9 0.4713490667612703 1101 10 0.6738216215021069 1101 11 0.5303235369911548 1101 12 0.6260737138088359 1101 13 0.4800837740018551 1101 14 0.975396379999502 1101 15 0.8901977531806153 1101 16 0.8421053269852052 1101 17 0.8125743196652948 1101 18 0.8680477875532233 1101 19 0.7896864181615868 1101 20 0.7579822520775892 1101 21 0.7555291616893527 1101 22 0.8198781278232801 1101 23 0.8742615804184339 1101 24 0.8316925777253132 1101 25 0.9343893532517598 1102 1 0.31684679751028355 1102 2 0.2913333153303803 1102 3 0.34068994648118434 1102 4 0.3570593204095118 1102 5 0.30130615226632074 1102 6 0.3070626653770816 1102 7 0.3877770813261705 1102 8 0.3645970251196786 1102 9 0.38455818474638903 1102 10 0.6075275160838558 1102 11 0.39890711267140233 1102 12 0.49389030068712003 1102 13 0.3557751499408468 1102 14 0.8194687033945058 1102 15 0.721243708355523 1102 16 0.7361558501049288 1102 17 0.7072273130102502 1102 18 0.7397547140077165 1102 19 0.6554056885832082 1102 20 0.6877341404095745 1102 21 0.7469963678268243 1102 22 0.7552935003918222 1102 23 0.8059526610997452 1102 24 0.7382315690797796 1102 25 0.7962459471278152 1103 1 0.29002906859867394 1103 2 0.32483586988677615 1103 3 0.2583619212631447 1103 4 0.289663530694348 1103 5 0.14187274278689443 1103 6 0.19835050176385494 1103 7 0.2595249451587094 1103 8 0.4137731685894295 1103 9 0.3583534249935383 1103 10 0.4723564499417776 1103 11 0.3015748517404262 1103 12 0.4012289324620113 1103 13 0.4667150359842722 1103 14 0.7168970106709551 1103 15 0.7088488238253419 1103 16 0.6036929454146858 1103 17 0.6238746322116455 1103 18 0.5969492762644037 1103 19 0.6102962441180324 1103 20 0.6145681764704918 1103 21 0.600141989288133 1103 22 0.6656224902192065 1103 23 0.7829922639792031 1103 24 0.7353515606774508 1103 25 0.7390715615134837 1104 1 0.2115624182228366 1104 2 0.23874344597777863 1104 3 0.2343506328880025 1104 4 0.19582438959010773 1104 5 0.15346013566455952 1104 6 0.12457693800078573 1104 7 0.1951871471478981 1104 8 0.38925128419941757 1104 9 0.24830076781065458 1104 10 0.36356475700502244 1104 11 0.30544392386965313 1104 12 0.4480995405194932 1104 13 0.2819648279611755 1104 14 0.6337997368648516 1104 15 0.552561733498095 1104 16 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0.03559335759507576 2213 6 -0.059496227894591414 2213 7 -0.05815201838120032 2213 8 0.08967166330246101 2213 9 -0.19709742615263082 2213 10 -1.56920153556149 2213 11 -0.45771998017073656 2213 12 -0.07365010374195435 2213 13 -0.2129148017118112 2213 14 -0.09118173993159928 2213 15 -0.08361557969578115 2213 16 -0.06094883865179668 2213 17 -0.1530837328853778 2213 18 -0.05150619274892421 2213 19 7.033066720723014E-4 2213 20 -0.07620951955730959 2213 21 -0.13142435645951878 2213 22 0.02552084237315455 2213 23 -0.9890939633235973 2213 24 -1.4237330972673377 2213 25 -1.3906144742607864 2214 1 -0.05403062234143263 2214 2 -0.32633966907038925 2214 3 -0.07348288181216134 2214 4 -0.06226496092218525 2214 5 -0.05980941530774686 2214 6 -0.20718233409827036 2214 7 -0.11991443289418413 2214 8 -0.08617702824118822 2214 9 -0.4388812179950037 2214 10 -0.10475859337776683 2214 11 -0.2059348576756932 2214 12 -0.19497337569870155 2214 13 -0.22580672187543627 2214 14 -0.19673828840628715 2214 15 -0.23055471115878134 2214 16 -0.28018361879116327 2214 17 -0.3364250555577115 2214 18 -0.15869447573982853 2214 19 -0.9308907767916921 2214 20 -0.3745990937193113 2214 21 -0.3208132747347179 2214 22 -0.5532120955997826 2214 23 -1.8595763344819431 2214 24 -1.3201621586144299 2214 25 -1.6149203586829373 2215 1 -0.3177454098713639 2215 2 -0.11614796073610023 2215 3 -1.0183285091224512 2215 4 -0.6657145585001061 2215 5 -0.23969846746960144 2215 6 -0.30839242875723016 2215 7 -0.1765443213149851 2215 8 -0.09028276456675854 2215 9 -0.31975796264228507 2215 10 -1.1833516887066793 2215 11 -0.43309216938423845 2215 12 -0.4232331794984532 2215 13 -0.38076940010508764 2215 14 -0.5883029594216538 2215 15 -0.9061851206711182 2215 16 -0.6126797505588542 2215 17 -0.37732235584478246 2215 18 -0.3591869020691121 2215 19 -0.9294019839479972 2215 20 -1.128285261871028 2215 21 -0.5304951489698695 2215 22 -0.37191198141486126 2215 23 -0.5207745050293013 2215 24 -1.7022367323976013 2215 25 -0.7111659372156467 2216 1 -0.4826075648835477 2216 2 -0.4211036095085845 2216 3 -1.4014870295784547 2216 4 -0.746417298033542 2216 5 -0.48034490553369835 2216 6 -0.4825862648346728 2216 7 -0.405994776243368 2216 8 -0.4352654446036013 2216 9 -0.30684131281149263 2216 10 -0.3913759799671226 2216 11 -0.49792304426482303 2216 12 -0.35332832963201355 2216 13 -1.0580633663676622 2216 14 -0.8302178825836464 2216 15 -1.145597166282407 2216 16 -0.9675340589005543 2216 17 -1.7516939510775273 2216 18 -0.9155336898950281 2216 19 -0.7439164770064508 2216 20 -0.681564905140533 2216 21 -0.45271386025888916 2216 22 -0.5482026794883197 2216 23 -0.4874545746661667 2216 24 -0.5412941764462218 2216 25 -0.4790062326672455 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5782.0 3 603.0 4 747.0 5 679.0 6 432.0 7 331.0 8 489.0 9 1043.0 10 1931.0 11 3622.0 12 6117.0 13 9462.0 14 13748.0 15 18557.0 16 22767.0 17 23853.0 18 23669.0 19 23708.0 20 23851.0 21 25729.0 22 27366.0 23 28407.0 24 30183.0 25 31640.0 26 33257.0 27 37332.0 28 41892.0 29 49342.0 30 60500.0 31 77447.0 32 104154.0 33 148963.0 34 219806.0 35 355456.0 36 661573.0 37 2349478.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.209106971955194 20.851530371366763 13.156247830119083 22.78311482655896 2 14.228066671304724 21.75872833485566 39.870530915260474 24.142674078579137 3 19.123617401953577 27.520718348305284 29.207029502532933 24.148634747208206 4 12.061198300201879 19.102927230536018 37.08699431724297 31.748880152019137 5 12.444110385553566 39.68316455474476 34.66227289896595 13.210452160735725 6 30.77832986436806 37.307705861372916 18.029708528357418 13.884255745901605 7 24.98890113367793 33.842070651185864 23.29390561138032 17.875122603755884 8 24.251224517926744 37.59181358861316 21.3138856224601 16.843076270999994 9 24.328711743018054 18.04930110410353 21.835908587115206 35.786078565763205 10 15.012863755552047 31.514865220756143 33.05496850350035 20.41730252019146 11 30.55045521476799 27.31786919982761 22.004067303548343 20.127608281856055 12 21.09339241046786 29.69265777741063 30.786795461284953 18.42715435083656 13 26.554647959165344 24.364091331975533 25.74777920263227 23.333481506226853 14 20.012921106052065 25.347164932890387 27.790053127834767 26.849860833222778 15 25.083825015476595 29.552060845132132 23.280234804317015 22.083879335074254 16 20.166110398925788 31.720661936539013 27.797532160012068 20.31569550452313 17 21.178720538720537 31.120359147025813 28.397845117845115 19.30307519640853 18 21.130764116200652 29.726190310506805 29.53502769792716 19.608017875365388 19 20.99760645742455 30.22936750811409 29.84372499244091 18.929301042020445 20 22.16793672434881 29.009929637105962 28.547038897681666 20.275094740863565 21 21.028830646745174 30.103890885951216 28.919061177445005 19.9482172898586 22 20.68451255276381 29.69439920807992 27.51370091324457 22.107387325911702 23 19.34712542947844 30.59150287037282 28.724537142000283 21.33683455814845 24 20.412066540416937 31.13515867839659 27.83680782014914 20.615966961037326 25 21.25106664029578 28.56703484885867 26.50747899776558 23.674419513079968 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 12166.0 1 12166.0 2 11409.0 3 10652.0 4 10652.0 5 10652.0 6 14059.0 7 17466.0 8 17466.0 9 17466.0 10 21990.0 11 26514.0 12 26514.0 13 26514.0 14 37929.5 15 49345.0 16 49345.0 17 49345.0 18 73211.0 19 97077.0 20 97077.0 21 97077.0 22 133103.5 23 169130.0 24 169130.0 25 169130.0 26 213945.5 27 258761.0 28 258761.0 29 258761.0 30 305309.5 31 351858.0 32 351858.0 33 351858.0 34 388936.0 35 426014.0 36 426014.0 37 426014.0 38 452174.0 39 478334.0 40 478334.0 41 478334.0 42 484719.5 43 491105.0 44 491105.0 45 491105.0 46 600293.0 47 709481.0 48 709481.0 49 709481.0 50 623589.0 51 537697.0 52 537697.0 53 537697.0 54 436720.5 55 335744.0 56 335744.0 57 335744.0 58 278612.0 59 221480.0 60 221480.0 61 221480.0 62 181401.0 63 141322.0 64 141322.0 65 141322.0 66 108242.5 67 75163.0 68 75163.0 69 75163.0 70 54664.5 71 34166.0 72 34166.0 73 34166.0 74 23764.0 75 13362.0 76 13362.0 77 13362.0 78 9045.5 79 4729.0 80 4729.0 81 4729.0 82 3176.5 83 1624.0 84 1624.0 85 1624.0 86 1057.5 87 491.0 88 491.0 89 491.0 90 335.5 91 180.0 92 180.0 93 180.0 94 108.0 95 36.0 96 36.0 97 36.0 98 27.5 99 19.0 100 19.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.30885437808417543 2 0.1797972900923763 3 0.19729313902860177 4 0.21819406996009783 5 0.24512109994901335 6 0.19482893495307707 7 0.19055018060375686 8 0.21304164325672792 9 0.21355688592706495 10 0.22242802059895392 11 0.19883886703961276 12 0.20553702175399358 13 0.21640192154153437 14 0.2058506477272422 15 0.19718112975244156 16 0.19998136165644692 17 0.19973494124889446 18 0.20318482695462908 19 0.20190792120640266 20 0.19868205405298844 21 0.19626265368792783 22 0.1998469505250547 23 0.2037224714801981 24 0.21911254602461158 25 0.2130192414014959 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 4463916.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.25298398406224 #Duplication Level Percentage of deduplicated Percentage of total 1 86.87859051025421 60.16601637164537 2 9.911699075421835 13.728294746500655 3 1.825680568306902 3.7930148147091454 4 0.5551563610693261 1.5378493832713727 5 0.24160499635504804 0.8365933471522778 6 0.1296588921691933 0.5387559109670638 7 0.0804121148380529 0.3898145230702976 8 0.059604954769331545 0.33022567824090177 9 0.04180152516038939 0.2605392317197639 >10 0.23890233024390314 3.042826981627975 >50 0.019443112809404747 0.9424039840628327 >100 0.013457898155403724 1.8223502527506972 >500 0.0014381727910022862 0.6734845339149579 >1k 0.0015689154806732498 2.4420233753294633 >5k 6.210290444331245E-4 2.7287064120316282 >10k+ 3.5954313098759846E-4 6.767100453005568 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 67056 1.5021788044398685 No Hit CCCATGTACTCTGCGTTGATACCAC 59821 1.340101381836038 No Hit GAGTACATGGGAAGCAGTGGTATCA 32011 0.7171057878329252 No Hit CATGTACTCTGCGTTGATACCACTG 22437 0.5026304258413464 No Hit GCGTTGATACCACTGCTTCCCATGT 19585 0.4387403347195601 No Hit GCTTCCCATGTACTCTGCGTTGATA 18940 0.42429113809489244 No Hit GTATCAACGCAGAGTACATGGGAAG 18504 0.4145239292137217 No Hit ACGCAGAGTACATGGGAAGCAGTGG 17110 0.38329574302025393 No Hit TATCAACGCAGAGTACATGGGAAGC 15360 0.3440924963641789 No Hit GGTATCAACGCAGAGTACATGGGAA 14465 0.3240428359315005 No Hit ACTCTGCGTTGATACCACTGCTTCC 13666 0.30614375360109825 No Hit GTGGTATCAACGCAGAGTACATGGG 9394 0.21044302804981097 No Hit CAGTGGTATCAACGCAGAGTACATG 7908 0.17715387117499523 No Hit GTATCAACGCAGAGTACTTTTTTTT 7749 0.1735919761931004 No Hit ACATGGGAAGCAGTGGTATCAACGC 7173 0.1606885075794437 No Hit GCAGTGGTATCAACGCAGAGTACAT 6769 0.15163815806569836 No Hit ATACCACTGCTTCCCATGTACTCTG 6656 0.14910674842447752 No Hit CATGGGAAGCAGTGGTATCAACGCA 6586 0.14753861855823452 No Hit GGGAAGCAGTGGTATCAACGCAGAG 6524 0.14614970353384787 No Hit GTACTCTGCGTTGATACCACTGCTT 6360 0.14247579927579282 No Hit GGTATCAACGCAGAGTACTTTTTTT 6291 0.14093007126478185 No Hit CTGCTTCCCATGTACTCTGCGTTGA 5901 0.13219334772428512 No Hit GTACATGGTAAGCAGTGGTATCAAC 5785 0.12959473251736817 No Hit GATACCACTGCTTCCCATGTACTCT 5721 0.1281610137825174 No Hit CCATGTACTCTGCGTTGATACCACT 5472 0.12258295182973873 No Hit CTTCCCATGTACTCTGCGTTGATAC 5382 0.12056678485885487 No Hit ATTCCATTCCATTCCATTCCATTCC 5366 0.1202083551751422 No Hit NNNNNNNNNNNNNNNNNNNNNNNNN 5185 0.11615361937814242 No Hit TACCACTGCTTCCCATGTACTCTGC 5176 0.11595200268105403 No Hit GTTGATACCACTGCTTCCCATGTAC 5150 0.11536955444502092 No Hit TATCAACGCAGAGTACTTTTTTTTT 4990 0.11178525760789405 No Hit GCAGAGTACATGGGAAGCAGTGGTA 4903 0.10983629620270631 No Hit AAGCAGTGGTATCAACGCAGAGTAC 4754 0.10649841977313193 No Hit ATGGGAAGCAGTGGTATCAACGCAG 4498 0.10076354483372896 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.2401855232042897E-5 0.0 5 0.0 0.0 0.0 2.2401855232042897E-5 0.0 6 0.0 0.0 0.0 2.2401855232042897E-5 0.0 7 0.0 0.0 0.0 2.2401855232042897E-5 0.0 8 0.0 0.0 0.0 4.4803710464085794E-5 0.0 9 0.0 0.0 0.0 4.4803710464085794E-5 0.0 10 0.0 0.0 0.0 6.720556569612868E-5 0.0 11 0.0 0.0 0.0 1.1200927616021448E-4 0.0 12 0.0 0.0 0.0 2.2401855232042896E-4 0.0 13 0.0 0.0 0.0 2.2401855232042896E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTGTCCG 90 5.4217526E-7 12.664892 9 GTACGGA 125 2.2122367E-7 10.650831 1 TAGACAG 655 0.0 9.723949 5 AGACAGT 665 0.0 9.712379 6 GTCCTAG 450 0.0 9.509669 1 GCACCGT 120 1.7060593E-5 9.498136 6 ATTAGAC 385 0.0 9.381631 3 CTAGGAC 450 0.0 9.082626 3 TGCACCG 105 4.4661816E-4 9.053571 5 ACCGAGT 105 4.5014295E-4 9.046453 8 TAGACTG 475 0.0 9.005921 5 TGTCGCC 285 0.0 8.998637 10 TCTGTCG 275 0.0 8.98066 8 CTACACG 85 0.0073986016 8.947159 4 GTACTAG 300 0.0 8.875692 1 GTGCTAG 400 0.0 8.796445 1 GTACAAA 890 0.0 8.761719 1 GTTCTAG 480 0.0 8.717197 1 CGCAAAA 240 1.0186341E-10 8.712984 2 TCTACAC 525 0.0 8.690353 3 >>END_MODULE