FastQCFastQC Report
Wed 25 May 2016
SRR1294988_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294988_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4463916
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC668911.4984824983265814No Hit
GTACATGGGAAGCAGTGGTATCAAC658761.4757446152660578No Hit
GAGTACATGGGAAGCAGTGGTATCA346200.7755522281333251No Hit
CATGTACTCTGCGTTGATACCACTG253850.5686710950654089No Hit
ACGCAGAGTACATGGGAAGCAGTGG213420.4781003943622595No Hit
GCGTTGATACCACTGCTTCCCATGT212690.4764650589303204No Hit
GCTTCCCATGTACTCTGCGTTGATA204260.4575802949697082No Hit
GTATCAACGCAGAGTACATGGGAAG189030.4234622694513069No Hit
GTATCAACGCAGAGTACTTTTTTTT184490.4132918271759594No Hit
TATCAACGCAGAGTACATGGGAAGC166370.37269966549549766No Hit
GGTATCAACGCAGAGTACTTTTTTT160920.3604906543940343No Hit
ACTCTGCGTTGATACCACTGCTTCC155740.3488864933838361No Hit
GGTATCAACGCAGAGTACATGGGAA154710.34657910229493566No Hit
TATCAACGCAGAGTACTTTTTTTTT125920.28208416108188417No Hit
GTGGTATCAACGCAGAGTACATGGG96050.21516981950377204No Hit
CAGTGGTATCAACGCAGAGTACATG92770.20782201098766195No Hit
ACATGGGAAGCAGTGGTATCAACGC90060.20175110821977835No Hit
CATGGGAAGCAGTGGTATCAACGCA83330.18667465964861346No Hit
ACGCAGAGTACTTTTTTTTTTTTTT77550.17372638732449266No Hit
GCAGTGGTATCAACGCAGAGTACAT76760.17195664076116127No Hit
GGGAAGCAGTGGTATCAACGCAGAG74450.16678181220255936No Hit
ATACCACTGCTTCCCATGTACTCTG70210.1572834255841732No Hit
GATACCACTGCTTCCCATGTACTCT64650.1448279940751573No Hit
CTGCTTCCCATGTACTCTGCGTTGA63040.14122129538279843No Hit
CCATGTACTCTGCGTTGATACCACT63030.1411988935275664No Hit
CTTCCCATGTACTCTGCGTTGATAC62900.14090766940954982No Hit
GTACTCTGCGTTGATACCACTGCTT62100.1391155209909864No Hit
GTACATGGTAAGCAGTGGTATCAAC58520.13109565681791502No Hit
TACCACTGCTTCCCATGTACTCTGC57350.128474639755766No Hit
GCAGAGTACATGGGAAGCAGTGGTA54770.12269496110589893No Hit
AAGCAGTGGTATCAACGCAGAGTAC54640.12240373698788239No Hit
ATGGGAAGCAGTGGTATCAACGCAG52620.11787856223100972No Hit
CCACTGCTTCCCATGTACTCTGCGT52000.11648964720662307No Hit
ATTCCATTCCATTCCATTCCATTCC51900.11626562865430262No Hit
GTTGATACCACTGCTTCCCATGTAC51120.1145182839462033No Hit
GTACTTTTTTTTTTTTTTTTTTTTT49040.10985869805793837No Hit
ACCATGTACTCTGCGTTGATACCAC45490.10190603945056313No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCTC855.3315212E-511.1757068
CGTAAGA600.00588010211.0829482
GGAACGA1701.5643309E-910.05813615
CAATCCG1554.0743544E-89.80578110
CTGTTCG800.00452079539.4993519
GAACGAT900.00111507089.49935116
GACTCCG2106.730261E-119.499357
CTAGGAC3850.09.3760863
GTCCTAG3800.09.2607771
AATCCGA1651.06834705E-79.21149211
AACGATT1252.7588492E-59.11937617
GTCTTAG4800.09.1147741
TAGGACT5400.08.9717094
CGCAAAA1701.6882223E-78.94086552
ACCGTGT2054.152753E-98.80427558
CTGTGCG1304.3760672E-58.7686319
CGTCTCA1752.6315138E-78.68512112
ATCCGAA1752.6315138E-78.68512112
TCAGTAC3950.08.6577333
GTCCTAC2201.5734258E-98.6464261
Produced by FastQC (version 0.11.5)