FastQCFastQC Report
Wed 25 May 2016
SRR1294987_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294987_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4177457
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC301850.7225687780867642No Hit
CCCATGTACTCTGCGTTGATACCAC271160.6491030308630347No Hit
GAGTACATGGGAAGCAGTGGTATCA142720.34164325329979456No Hit
CATGTACTCTGCGTTGATACCACTG101480.24292290740515105No Hit
GCTTCCCATGTACTCTGCGTTGATA87430.2092900058576306No Hit
GCGTTGATACCACTGCTTCCCATGT86480.2070158950768374No Hit
GTATCAACGCAGAGTACATGGGAAG80850.19353879644961036No Hit
ACGCAGAGTACATGGGAAGCAGTGG78080.18690796817298178No Hit
GTATCAACGCAGAGTACTTTTTTTT76420.18293425880864841No Hit
GGTATCAACGCAGAGTACATGGGAA70940.16981623030470452No Hit
TATCAACGCAGAGTACATGGGAAGC70740.169337470140327No Hit
GGTATCAACGCAGAGTACTTTTTTT62710.15011524954056976No Hit
ACTCTGCGTTGATACCACTGCTTCC60880.1457345940365155No Hit
TATCAACGCAGAGTACTTTTTTTTT49470.1184213266587783No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN49180.1177271244204309No Hit
GTGGTATCAACGCAGAGTACATGGG46810.11205381647255734No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGCGC951.3627587E-510.999168
GAACCGC700.001493864110.8557916
AATACCG803.7549096E-410.6932275
AGCGCCC803.7762098E-410.68668310
ATACCGT800.004522789.4988186
GTCTAAT4150.09.39598751
GTCTTAT4400.09.2944221
GTGCTAC3500.09.2388411
GTATAGG4850.09.2164311
CTGTCGC3200.09.2024219
GAGTACG850.00737157328.9511271
GTCTTAC4250.08.9511261
TAGGACT6350.08.8319264
TCGCCCA3250.08.766561512
TCTGTCG3150.08.7453638
TATACTG8700.08.7403145
TTAGACA5250.08.6907864
TGTCGCC3500.08.6850510
TTACACT7900.08.6632844
TGGACAG5600.08.6564225
Produced by FastQC (version 0.11.5)