##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294987_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4177457
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.266725187117427	33.0	33.0	33.0	27.0	33.0
2	31.13257802533934	33.0	33.0	33.0	27.0	33.0
3	31.237239545493825	33.0	33.0	33.0	27.0	33.0
4	34.82101718820804	37.0	37.0	37.0	33.0	37.0
5	35.0482839200978	37.0	37.0	37.0	33.0	37.0
6	35.06821877520223	37.0	37.0	37.0	33.0	37.0
7	34.908735146765125	37.0	37.0	37.0	33.0	37.0
8	34.84380162381085	37.0	37.0	37.0	33.0	37.0
9	34.7373964591377	37.0	37.0	37.0	27.0	37.0
10	34.648460055962275	37.0	37.0	37.0	27.0	37.0
11	34.84369366339378	37.0	37.0	37.0	33.0	37.0
12	34.75147344425089	37.0	37.0	37.0	27.0	37.0
13	34.67664873630058	37.0	37.0	37.0	27.0	37.0
14	36.088785354343564	40.0	37.0	40.0	27.0	40.0
15	36.25968477952017	40.0	37.0	40.0	27.0	40.0
16	36.335265928530205	40.0	37.0	40.0	27.0	40.0
17	36.26338008984892	40.0	37.0	40.0	27.0	40.0
18	36.193864113981306	40.0	37.0	40.0	27.0	40.0
19	36.29252964183713	40.0	37.0	40.0	27.0	40.0
20	36.30867774342142	40.0	37.0	40.0	27.0	40.0
21	36.30292663694683	40.0	37.0	40.0	27.0	40.0
22	36.201112064109815	40.0	37.0	40.0	27.0	40.0
23	36.104883664870755	40.0	37.0	40.0	27.0	40.0
24	36.17456002539344	40.0	37.0	40.0	27.0	40.0
25	35.05346817453776	37.0	33.0	40.0	27.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.4362077266201183
1101	2	0.39501682040826935
1101	3	0.4265812489463805
1101	4	0.5527700591526568
1101	5	0.3860460852699461
1101	6	0.32973477853092703
1101	7	0.3134568040665755
1101	8	0.3869930073075665
1101	9	0.5002473702181618
1101	10	0.6437266045853178
1101	11	0.5052011540415293
1101	12	0.5036789456457171
1101	13	0.4999991379316029
1101	14	0.9537280550255431
1101	15	0.8348237962292941
1101	16	0.7195529317723839
1101	17	0.7546741936506152
1101	18	0.8099498279899535
1101	19	0.6954086127584276
1101	20	0.6821256678681848
1101	21	0.6910465565110968
1101	22	0.7073394410123015
1101	23	0.8819894844335749
1101	24	0.8158990024679795
1101	25	0.8788128142437586
1102	1	0.2803423621615657
1102	2	0.28135132480552727
1102	3	0.34093581303858045
1102	4	0.35745428366750787
1102	5	0.20429697932141266
1102	6	0.17447771845665727
1102	7	0.26272928762052317
1102	8	0.3251673673089641
1102	9	0.29489433842910984
1102	10	0.5417900753767242
1102	11	0.24419419815888688
1102	12	0.396059683671794
1102	13	0.429825768062571
1102	14	0.7552103368345584
1102	15	0.6289800655702535
1102	16	0.6003663719925996
1102	17	0.55639387014498
1102	18	0.6080203800682966
1102	19	0.5413840709520059
1102	20	0.5704203161621919
1102	21	0.5949798526598755
1102	22	0.6450041511225066
1102	23	0.7173444160040106
1102	24	0.6895984788894793
1102	25	0.676839537211336
1103	1	0.3520105024175493
1103	2	0.3587673147355872
1103	3	0.3616080886319786
1103	4	0.4260537360866721
1103	5	0.22372877752140852
1103	6	0.1630310871561349
1103	7	0.20427910422676376
1103	8	0.3417044602763539
1103	9	0.320089697365745
1103	10	0.49437857741745717
1103	11	0.36239347510947084
1103	12	0.46417658202632595
1103	13	0.4192326421709893
1103	14	0.6967654162597867
1103	15	0.8037500029860496
1103	16	0.634410077537261
1103	17	0.6134260333988664
1103	18	0.6290095857169717
1103	19	0.6412866159274415
1103	20	0.6591632856551755
1103	21	0.5708509111180291
1103	22	0.624522497928659
1103	23	0.7246329559370395
1103	24	0.6599957412329545
1103	25	0.7666603784698509
1104	1	0.3975283083628369
1104	2	0.3095993079747821
1104	3	0.3129841392865913
1104	4	0.30659239151357554
1104	5	0.260265664061869
1104	6	0.24113226549324907
1104	7	0.27297930937370296
1104	8	0.3511357723656445
1104	9	0.36503537673957
1104	10	0.5168979090083852
1104	11	0.4055214685695816
1104	12	0.4478141555045312
1104	13	0.4554044027085524
1104	14	0.772488811750037
1104	15	0.5758703704877135
1104	16	0.5368030298336919
1104	17	0.6740747789307804
1104	18	0.6570021757624076
1104	19	0.5530493702757937
1104	20	0.6040135223326359
1104	21	0.49754501897849224
1104	22	0.6066638567708793
1104	23	0.6400036182215914
1104	24	0.6027252812960029
1104	25	0.8207722639416417
1105	1	0.17237697117711548
1105	2	0.09873158085919442
1105	3	0.08136052708843522
1105	4	0.21576440756684434
1105	5	0.12499144339110302
1105	6	0.07885949846220797
1105	7	0.06318679855282028
1105	8	0.16497416503921158
1105	9	0.21702304636085046
1105	10	0.2728782600705202
1105	11	0.20742994341411958
1105	12	0.22037054320792748
1105	13	0.2308437604868132
1105	14	0.4769860072742347
1105	15	0.3438807546022602
1105	16	0.3127989282901993
1105	17	0.20477845945265472
1105	18	0.2872649176396891
1105	19	0.26505422306211557
1105	20	0.3508729373098589
1105	21	0.36258788033518385
1105	22	0.27690066865383756
1105	23	0.3624286330930957
1105	24	0.3886985478050775
1105	25	0.3471812891548538
1106	1	0.10208474504097609
1106	2	-0.145157077847351
1106	3	-0.15165499154989348
1106	4	0.047394853533049286
1106	5	-0.3062131951283078
1106	6	-0.3103984431140887
1106	7	-0.6205627952310522
1106	8	-0.28335334959965053
1106	9	-0.00260376799646167
1106	10	-0.3829159187565452
1106	11	-0.4436545741625437
1106	12	-0.8482065662746763
1106	13	-0.2484766792652806
1106	14	-0.4688160781941306
1106	15	-0.20809240814720198
1106	16	-0.21143323965736727
1106	17	-0.2610847920145787
1106	18	-0.3575965237300949
1106	19	-0.5996919075509055
1106	20	-0.16493643192148966
1106	21	-0.04725936935537334
1106	22	0.18409073899506723
1106	23	-0.5851387091030489
1106	24	0.06862583735984629
1106	25	0.1863810111750226
1107	1	-1.0087443390201685
1107	2	-1.055000657779182
1107	3	-0.8618561219325471
1107	4	-0.8722117938049507
1107	5	-0.9436302790970075
1107	6	-0.8124509543863923
1107	7	-0.9580916314029224
1107	8	-0.9628113828583551
1107	9	-1.106114088960176
1107	10	-0.9265443216134912
1107	11	-0.9304112777618343
1107	12	-0.9459565705589341
1107	13	-0.8532509729883699
1107	14	-1.224873659192859
1107	15	-0.9767114996307171
1107	16	-1.0689136488263955
1107	17	-1.0968669104357502
1107	18	-1.334179579058599
1107	19	-1.17469543321657
1107	20	-1.247849947696423
1107	21	-1.2708331098516013
1107	22	-1.122564114136786
1107	23	-0.8995056119617999
1107	24	-0.88360591293781
1107	25	-0.8614389640124713
1108	1	-0.05190655361644403
1108	2	-0.1699493797823095
1108	3	-0.10465473396940439
1108	4	-0.17081420364724664
1108	5	-0.4777542869340934
1108	6	-0.07161896504439369
1108	7	-0.02077906816386843
1108	8	0.19734020567070587
1108	9	-0.23211425907577166
1108	10	-0.49606753795811187
1108	11	-0.042919332634269836
1108	12	0.12157125692014148
1108	13	0.17831638398827465
1108	14	-0.22365373919866016
1108	15	0.09515381605525874
1108	16	-0.7861826749715775
1108	17	-0.7150441066467224
1108	18	-0.5406908155797936
1108	19	-0.08463013814481712
1108	20	-0.8828971703984649
1108	21	-0.1843814615798891
1108	22	-0.27579601409227195
1108	23	-0.13123285554275554
1108	24	-0.295641903920675
1108	25	-1.0322565063953562
1109	1	0.09020877098377511
1109	2	-0.05565573668741308
1109	3	0.08896870652418798
1109	4	0.14797349183248087
1109	5	0.04218005332932506
1109	6	-0.05085187504079869
1109	7	0.10023568891317325
1109	8	0.24899973060774983
1109	9	0.14633071250311502
1109	10	0.1992686948644007
1109	11	0.1470938473251735
1109	12	0.21242135846040355
1109	13	0.199035102219014
1109	14	0.4682302055895491
1109	15	0.39210529745878375
1109	16	0.28319094383673615
1109	17	0.3108023341932977
1109	18	0.3886925099508147
1109	19	0.29354901345924134
1109	20	0.21058168524766785
1109	21	0.29090334971040477
1109	22	0.2910775572883253
1109	23	0.3644924014188007
1109	24	0.3228675459726844
1109	25	0.3793090136654911
1110	1	0.041702621725917766
1110	2	0.011341871599903186
1110	3	0.12714399491330397
1110	4	-0.9108418300082235
1110	5	-0.16327226260232663
1110	6	0.07046855722637702
1110	7	0.08472719657028449
1110	8	0.1805004236400478
1110	9	0.15861232768072853
1110	10	-0.013921065987716474
1110	11	0.08081837291102545
1110	12	0.13615764514256767
1110	13	0.19275388338765254
1110	14	0.21133117949728586
1110	15	0.10006637221462
1110	16	0.18226365598843586
1110	17	0.2701777698068213
1110	18	0.3681095102927543
1110	19	0.2342438628950987
1110	20	0.21587435509684383
1110	21	0.2864441234010613
1110	22	-0.4240149038679135
1110	23	0.09230712723982037
1110	24	0.20089366308157963
1110	25	0.2390412270978146
1111	1	-0.02498464626434682
1111	2	-0.2498039413503932
1111	3	-0.1338396943849176
1111	4	-0.0931956144490158
1111	5	-0.03483653318765789
1111	6	-0.0632162159188141
1111	7	-0.0333406090963706
1111	8	0.06806511457382669
1111	9	0.07241526539672094
1111	10	0.06984292420447247
1111	11	-0.0826155481332762
1111	12	0.09085252809736488
1111	13	-0.10971389533882103
1111	14	-0.18065778290315393
1111	15	-0.0440289305530186
1111	16	-0.07588102129950869
1111	17	-0.07154639763732007
1111	18	-0.12273293798642726
1111	19	-0.05529908629414848
1111	20	-0.06819919499260152
1111	21	-0.10877620131412868
1111	22	-0.059127173002998745
1111	23	0.04742718362211207
1111	24	-0.010495526796603372
1111	25	0.003006232026926625
1112	1	-3.083473705416395E-4
1112	2	-0.2953364449572504
1112	3	-0.11850054013168432
1112	4	-0.17469571409251472
1112	5	-0.054629643851072274
1112	6	-0.11015223343790126
1112	7	-0.033639617517209786
1112	8	0.059290007801884315
1112	9	-0.03002573203748682
1112	10	0.2055002617119328
1112	11	0.04332310286407193
1112	12	-0.019636092851079923
1112	13	0.006166317212311867
1112	14	-0.12648306762963557
1112	15	-0.0774787109309969
1112	16	-0.11654296048645563
1112	17	-0.16544944672838113
1112	18	-0.06318421984087053
1112	19	-0.0188927516170736
1112	20	-0.8604653544329324
1112	21	-1.1458444956612226
1112	22	-1.6117667594514202
1112	23	-0.39364650552263925
1112	24	-0.24177856257944796
1112	25	-1.419659069368329
1113	1	-0.27923468920754146
1113	2	-0.254734721854593
1113	3	-0.26196674214941496
1113	4	-0.6086517377653422
1113	5	-0.3651374842684305
1113	6	-0.26344009142808744
1113	7	-0.24002640801429465
1113	8	-0.08135168562471051
1113	9	-0.2290267112744928
1113	10	-0.10875157676075276
1113	11	-0.16804648920194154
1113	12	-0.2896003899912145
1113	13	-0.18434023331999327
1113	14	-0.5952292333842664
1113	15	-0.592250387885862
1113	16	-0.5045375317339094
1113	17	-0.43061447439112044
1113	18	-0.9613502554386031
1113	19	-1.1323351806874982
1113	20	-1.2345542070891042
1113	21	-1.1017300785023423
1113	22	-0.5552280100872977
1113	23	-1.927770725938558
1113	24	-1.1351132122168224
1113	25	-0.5651973179983756
1114	1	-0.2881299893940863
1114	2	-0.48055957092737955
1114	3	-0.3680392898956164
1114	4	-0.568307851612218
1114	5	-0.6575804799157297
1114	6	-0.3990854251673568
1114	7	-0.37799085803935384
1114	8	-0.22967604604372127
1114	9	-1.0438009592355826
1114	10	-0.28886320299751134
1114	11	-0.34593327705474053
1114	12	-0.34337572455016385
1114	13	-0.3802629242395028
1114	14	-0.7273317897265699
1114	15	-1.2896986393760699
1114	16	-1.0821690786811544
1114	17	-1.492306300346911
1114	18	-1.690448559367141
1114	19	-1.659450586147031
1114	20	-0.8350247258787817
1114	21	-0.6891507642149435
1114	22	-0.4355264506887693
1114	23	-0.356997295853283
1114	24	-0.9334506784651779
1114	25	-0.6719293606852474
1115	1	-0.7023535757933104
1115	2	-0.4920348594648516
1115	3	-0.46278265834970256
1115	4	-0.6603487207902745
1115	5	-0.40597682776555644
1115	6	-0.37585036105571845
1115	7	-0.32454199490312874
1115	8	-0.14504179048761756
1115	9	-0.4216394190763495
1115	10	-0.27354096083143276
1115	11	-0.4078051252126613
1115	12	-0.4776960875709264
1115	13	-0.4431670903799301
1115	14	-0.4571342944882204
1115	15	-1.377549256891541
1115	16	-0.8174444506511378
1115	17	-0.6549904036450442
1115	18	-2.174912377846553
1115	19	-1.0695249992808868
1115	20	-0.9818257775878081
1115	21	-0.9667391816377986
1115	22	-0.7227633161264038
1115	23	-0.36007524147415637
1115	24	-0.5415747607393442
1115	25	-0.6366407211583862
1116	1	-1.5757801281234087
1116	2	-0.8658975543040803
1116	3	-1.7019850605812685
1116	4	-1.0926651783822052
1116	5	-0.951147314450175
1116	6	-0.6507376891118071
1116	7	-1.2655471482371752
1116	8	-3.022982223850363
1116	9	-1.5541619884815105
1116	10	-0.8824790809580918
1116	11	-0.7033387587171944
1116	12	-0.779780478129652
1116	13	-1.7747682440889179
1116	14	-1.8421872554196312
1116	15	-1.587221643618932
1116	16	-1.1237610827862667
1116	17	-1.820407578049256
1116	18	-2.642556594163757
1116	19	-1.5142728112957116
1116	20	-1.0976905691002727
1116	21	-0.8844138352719071
1116	22	-0.7208776031336015
1116	23	-0.6405203697256994
1116	24	-0.7814093972208127
1116	25	-0.6431691754706321
1201	1	0.3054606948162899
1201	2	0.26517654450558226
1201	3	0.3072740959851039
1201	4	0.4539944319438405
1201	5	0.28557189703992947
1201	6	0.13409065616023952
1201	7	0.15828258821091623
1201	8	0.2757612572723218
1201	9	0.2616934401188473
1201	10	0.4930569558056064
1201	11	0.30060632237848495
1201	12	0.3792173205814038
1201	13	0.32321864944150747
1201	14	0.7471301039016041
1201	15	0.6745170371025537
1201	16	0.5581492888289219
1201	17	0.5263126701924818
1201	18	0.4868865496529864
1201	19	0.46945223734723385
1201	20	0.47246303891598984
1201	21	0.5257032935529224
1201	22	0.5308958449335819
1201	23	0.5941068939491743
1201	24	0.6062859279937172
1201	25	0.7784665051809299
1202	1	0.32670287890027083
1202	2	0.3236743160165183
1202	3	0.373806541500155
1202	4	0.485240722886914
1202	5	0.328965219913961
1202	6	0.1601776323165467
1202	7	0.17812092247311995
1202	8	0.3028383955618992
1202	9	0.305247637111016
1202	10	0.40307381358555006
1202	11	0.24259640641236757
1202	12	0.27338975128413523
1202	13	0.2185070513632823
1202	14	0.6172245191576593
1202	15	0.4700647608772286
1202	16	0.4730312073324683
1202	17	0.5571653030185644
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2210	22	0.5351274942741995
2210	23	0.5714768526448992
2210	24	0.5287823660396995
2210	25	0.638280228178445
2211	1	0.1750978846903095
2211	2	-0.0776462664277986
2211	3	0.12078751222135864
2211	4	0.065146150905953
2211	5	0.09870936099262195
2211	6	-0.03135925845464982
2211	7	0.1287294410240989
2211	8	0.19087194176861288
2211	9	0.08192562634253875
2211	10	0.286803098154337
2211	11	0.10753394663923643
2211	12	0.20906910895083541
2211	13	0.06118841956075727
2211	14	0.021552897130526105
2211	15	0.06868650575957957
2211	16	0.001356685402519986
2211	17	4.1014395073091237E-4
2211	18	0.10993282103999746
2211	19	0.058547967410618185
2211	20	0.07262646470973522
2211	21	0.08975416664063829
2211	22	0.05196342393122677
2211	23	0.15960016990189985
2211	24	0.23633961531250236
2211	25	0.028592576507534773
2212	1	0.10268262888131119
2212	2	0.09255744075201733
2212	3	0.08028758740043074
2212	4	0.08248417805324948
2212	5	0.13867060335032022
2212	6	0.15819564513586926
2212	7	0.12619516278337528
2212	8	0.25402108537135604
2212	9	0.14753207502074162
2212	10	0.2848819465164638
2212	11	0.13479139600317325
2212	12	0.22389883981322356
2212	13	0.10533710154066966
2212	14	0.287348780637636
2212	15	0.2096071653398397
2212	16	0.1197152587000403
2212	17	0.17211859977608412
2212	18	0.16889013705295497
2212	19	0.19676023506125517
2212	20	0.27386810390225236
2212	21	0.20027688900662355
2212	22	-0.41052928836793967
2212	23	0.19194945575260647
2212	24	0.2082668253718154
2212	25	0.2842497258814731
2213	1	-0.10373632670783195
2213	2	-0.8135672730765293
2213	3	-0.270263208431718
2213	4	-0.10261704096313906
2213	5	-0.01876215107412804
2213	6	-0.02682057964229756
2213	7	-0.05221435620644854
2213	8	0.06473487444683457
2213	9	-0.24867219424398712
2213	10	-1.5802664853481332
2213	11	-0.42094977406980405
2213	12	-0.23494562330139246
2213	13	-0.15153942985856617
2213	14	-0.0028450568074376292
2213	15	-0.06104883926261806
2213	16	-0.12458654099697952
2213	17	-0.17917471401327134
2213	18	-0.003354314502445277
2213	19	-0.11309954849283343
2213	20	-0.14503165853678013
2213	21	-0.108570799742985
2213	22	0.007326039995483313
2213	23	-0.9215942434337947
2213	24	-1.329867601681194
2213	25	-1.4215515703594335
2214	1	-0.008838059195813486
2214	2	-0.11813231876461217
2214	3	0.008767431610511522
2214	4	0.03136984600019588
2214	5	0.03492197278578857
2214	6	-0.08109483594962086
2214	7	0.031909224407954184
2214	8	0.09184112512930653
2214	9	-0.24552712818766054
2214	10	0.07092038529234657
2214	11	-0.10397362792775056
2214	12	-0.0549428019199496
2214	13	-0.03820699372096925
2214	14	-0.003578188810486438
2214	15	-0.011702471275739867
2214	16	-0.1598341755434305
2214	17	-0.14046857678573588
2214	18	0.01141435840438021
2214	19	-0.5670216024190893
2214	20	-0.24316714189777144
2214	21	-0.14828551203549978
2214	22	-0.4310705297895794
2214	23	-1.7949751913953662
2214	24	-1.2658409728350293
2214	25	-1.5020778793716758
2215	1	-0.5398829001072833
2215	2	-0.24605081520454775
2215	3	-1.224103758811232
2215	4	-1.0494542593327267
2215	5	-0.47176288502483743
2215	6	-0.29916259537691303
2215	7	-0.2057549706920625
2215	8	-0.14456183454914395
2215	9	-0.35708948647309313
2215	10	-1.3171672577787987
2215	11	-0.6636369625659881
2215	12	-0.5809271973292738
2215	13	-0.6065544032271362
2215	14	-0.7788298351219325
2215	15	-1.0263167557164934
2215	16	-0.8250738451571138
2215	17	-0.5154791387603055
2215	18	-0.4782213406820617
2215	19	-0.9791688421584723
2215	20	-1.3861406187447542
2215	21	-0.7587451614167051
2215	22	-0.41738027277507683
2215	23	-0.7319781487482473
2215	24	-1.7838862517292995
2215	25	-0.9440982591376468
2216	1	-0.6282314167826364
2216	2	-0.4543391048358707
2216	3	-1.378676902457041
2216	4	-0.8852068143385594
2216	5	-0.7700222114432904
2216	6	-0.5672901680856199
2216	7	-0.3571330752928361
2216	8	-0.5568899252797266
2216	9	-0.32804597356193455
2216	10	-0.4757010104881232
2216	11	-0.4755231210431319
2216	12	-0.4637015294398381
2216	13	-1.0732336944191303
2216	14	-0.8787727427206846
2216	15	-1.2126306756860004
2216	16	-0.9913621118225464
2216	17	-1.8727030499604709
2216	18	-0.8824733143889318
2216	19	-0.6153020467485035
2216	20	-0.6458488149290744
2216	21	-0.3645034760264565
2216	22	-0.4222269422070468
2216	23	-0.36543141507220867
2216	24	-0.4918800541617898
2216	25	-0.4197986130742706
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	5445.0
3	553.0
4	712.0
5	614.0
6	417.0
7	290.0
8	493.0
9	1002.0
10	1881.0
11	3499.0
12	5978.0
13	9281.0
14	12714.0
15	16626.0
16	19454.0
17	20527.0
18	21419.0
19	22091.0
20	22671.0
21	24495.0
22	26072.0
23	27743.0
24	29543.0
25	31151.0
26	32648.0
27	36965.0
28	41287.0
29	48565.0
30	59478.0
31	75003.0
32	101142.0
33	143640.0
34	208343.0
35	333361.0
36	614312.0
37	2178042.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.72849276142539	20.64564757454744	13.6380215643274	22.987838099699776
2	14.829913211972384	20.81406642317737	40.517011076924554	23.8390092879257
3	18.608993115411305	26.040933356711303	29.770815129257233	25.57925839862016
4	11.998681495207466	17.928786347046007	38.10015838370889	31.972373774037642
5	12.541277092422531	39.11111441730459	35.16161566956537	13.1859928207075
6	31.226160286343998	37.95631397581964	17.516215742347967	13.301309995488397
7	26.136157865665865	33.161105167108815	22.773715310031086	17.929021657194237
8	24.00476243938997	38.06437017866585	20.711405576938393	17.219461805005793
9	23.99765764871016	17.795176685112324	21.853964808594828	36.35320085758269
10	14.614445780279475	31.09066598400725	33.67508690591896	20.619801329794317
11	31.599912403093523	26.298548582582754	22.708970311796538	19.392568702527193
12	20.823932232435034	28.46236769094889	31.244989611013906	19.468710465602165
13	26.74047869461579	23.97217978589058	26.192418269544486	23.09492324994914
14	20.045495575779544	24.434861518673312	28.0503996754908	27.469243230056346
15	23.61269781724935	30.59846891086012	23.740060716844454	22.048772555046078
16	20.86055179050555	31.62965819998148	26.72978422401672	20.78000578549625
17	20.435823514695393	30.842760193205756	28.379715458786908	20.341700833311947
18	20.56939404464692	30.00270096006417	29.41000094029871	20.017904054990204
19	20.864755302625976	29.700918102388506	29.689308394849988	19.74501820013553
20	21.91602472648983	29.08228859148619	28.8499860402597	20.15170064176428
21	21.549382257070285	29.4108476186136	28.652190702795018	20.387579421521103
22	20.847469172995815	29.22398557760576	28.573121308531015	21.355423940867414
23	20.424297754426096	29.64068322862176	28.773814366913957	21.161204650038187
24	20.766565753086486	29.75709487883773	28.653682969819734	20.822656398256058
25	21.386574367834484	28.730434457185805	27.59455524531791	22.288435929661805
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	9585.0
1	9585.0
2	8406.0
3	7227.0
4	7227.0
5	7227.0
6	9781.0
7	12335.0
8	12335.0
9	12335.0
10	16886.5
11	21438.0
12	21438.0
13	21438.0
14	33028.0
15	44618.0
16	44618.0
17	44618.0
18	67899.0
19	91180.0
20	91180.0
21	91180.0
22	127894.0
23	164608.0
24	164608.0
25	164608.0
26	210153.0
27	255698.0
28	255698.0
29	255698.0
30	300652.5
31	345607.0
32	345607.0
33	345607.0
34	382246.5
35	418886.0
36	418886.0
37	418886.0
38	439918.0
39	460950.0
40	460950.0
41	460950.0
42	462490.5
43	464031.0
44	464031.0
45	464031.0
46	505835.5
47	547640.0
48	547640.0
49	547640.0
50	501772.0
51	455904.0
52	455904.0
53	455904.0
54	395132.0
55	334360.0
56	334360.0
57	334360.0
58	287429.0
59	240498.0
60	240498.0
61	240498.0
62	198828.0
63	157158.0
64	157158.0
65	157158.0
66	120917.5
67	84677.0
68	84677.0
69	84677.0
70	61329.5
71	37982.0
72	37982.0
73	37982.0
74	26688.0
75	15394.0
76	15394.0
77	15394.0
78	10307.0
79	5220.0
80	5220.0
81	5220.0
82	3478.0
83	1736.0
84	1736.0
85	1736.0
86	1114.0
87	492.0
88	492.0
89	492.0
90	331.5
91	171.0
92	171.0
93	171.0
94	103.0
95	35.0
96	35.0
97	35.0
98	31.0
99	27.0
100	27.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.30865667797418384
2	0.1801813878634777
3	0.19844608813447992
4	0.21781193678355038
5	0.24402405578321928
6	0.19643529544409433
7	0.19121680965237942
8	0.21556176401097607
9	0.21534632193700615
10	0.2260705496190625
11	0.2001217487098012
12	0.20632169283849003
13	0.21833857296436565
14	0.20826067150421895
15	0.19791945195366464
16	0.20134258712896386
17	0.2017974092851225
18	0.20512479242754622
19	0.20423908612344782
20	0.19928391842214058
21	0.19741675378106824
22	0.20072019891527307
23	0.20455028023029323
24	0.22032542764653232
25	0.21467605770687767
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4177457.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.88559209797504
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.5607806439362	68.09048056467921
2	8.827322042719404	13.573877637879757
3	1.509303438608887	3.4813106559886204
4	0.41622925665018407	1.2800813138419769
5	0.18979610062714852	0.7296292787302582
6	0.10310548106649144	0.47563955762062493
7	0.06737959158587516	0.36263638560798234
8	0.053883420981813895	0.331428698116097
9	0.03887305658399989	0.2689900174907027
>10	0.20650935765492098	2.910417362962028
>50	0.014673679144904682	0.7977112987840426
>100	0.009574110030786107	1.3950951460411745
>500	9.088390124723978E-4	0.46467954846646836
>1k	0.0012535708655169208	2.243617637734516
>5k	2.8205344474130345E-4	1.6253059745914185
>10k+	1.2535708655169042E-4	1.9690989214650987
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	30185	0.7225687780867642	No Hit
CCCATGTACTCTGCGTTGATACCAC	27116	0.6491030308630347	No Hit
GAGTACATGGGAAGCAGTGGTATCA	14272	0.34164325329979456	No Hit
CATGTACTCTGCGTTGATACCACTG	10148	0.24292290740515105	No Hit
GCTTCCCATGTACTCTGCGTTGATA	8743	0.2092900058576306	No Hit
GCGTTGATACCACTGCTTCCCATGT	8648	0.2070158950768374	No Hit
GTATCAACGCAGAGTACATGGGAAG	8085	0.19353879644961036	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	7808	0.18690796817298178	No Hit
GTATCAACGCAGAGTACTTTTTTTT	7642	0.18293425880864841	No Hit
GGTATCAACGCAGAGTACATGGGAA	7094	0.16981623030470452	No Hit
TATCAACGCAGAGTACATGGGAAGC	7074	0.169337470140327	No Hit
GGTATCAACGCAGAGTACTTTTTTT	6271	0.15011524954056976	No Hit
ACTCTGCGTTGATACCACTGCTTCC	6088	0.1457345940365155	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4947	0.1184213266587783	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	4918	0.1177271244204309	No Hit
GTGGTATCAACGCAGAGTACATGGG	4681	0.11205381647255734	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.393800821887574E-5	0.0
4	0.0	0.0	0.0	4.787601643775148E-5	0.0
5	0.0	0.0	0.0	4.787601643775148E-5	0.0
6	0.0	0.0	0.0	4.787601643775148E-5	0.0
7	0.0	2.393800821887574E-5	0.0	7.181402465662723E-5	0.0
8	2.393800821887574E-5	2.393800821887574E-5	0.0	7.181402465662723E-5	0.0
9	2.393800821887574E-5	2.393800821887574E-5	0.0	7.181402465662723E-5	0.0
10	2.393800821887574E-5	2.393800821887574E-5	0.0	9.575203287550297E-5	0.0
11	2.393800821887574E-5	2.393800821887574E-5	0.0	1.1969004109437871E-4	0.0
12	2.393800821887574E-5	2.393800821887574E-5	0.0	1.1969004109437871E-4	0.0
13	2.393800821887574E-5	2.393800821887574E-5	0.0	1.1969004109437871E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAGCGC	95	1.3627587E-5	10.99916	8
GAACCGC	70	0.0014938641	10.855791	6
AATACCG	80	3.7549096E-4	10.693227	5
AGCGCCC	80	3.7762098E-4	10.686683	10
ATACCGT	80	0.00452278	9.498818	6
GTCTAAT	415	0.0	9.3959875	1
GTCTTAT	440	0.0	9.294422	1
GTGCTAC	350	0.0	9.238841	1
GTATAGG	485	0.0	9.216431	1
CTGTCGC	320	0.0	9.202421	9
GAGTACG	85	0.0073715732	8.951127	1
GTCTTAC	425	0.0	8.951126	1
TAGGACT	635	0.0	8.831926	4
TCGCCCA	325	0.0	8.7665615	12
TCTGTCG	315	0.0	8.745363	8
TATACTG	870	0.0	8.740314	5
TTAGACA	525	0.0	8.690786	4
TGTCGCC	350	0.0	8.68505	10
TTACACT	790	0.0	8.663284	4
TGGACAG	560	0.0	8.656422	5
>>END_MODULE