FastQCFastQC Report
Wed 25 May 2016
SRR1294987_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294987_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4177457
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC304330.7285054041250455No Hit
GTACATGGGAAGCAGTGGTATCAAC296460.7096661916567902No Hit
GTATCAACGCAGAGTACTTTTTTTT174520.4177661194358194No Hit
GGTATCAACGCAGAGTACTTTTTTT158240.37879504205548975No Hit
GAGTACATGGGAAGCAGTGGTATCA157000.37582672903634917No Hit
TATCAACGCAGAGTACTTTTTTTTT119970.2871842846018523No Hit
CATGTACTCTGCGTTGATACCACTG113410.27148095121026977No Hit
ACGCAGAGTACATGGGAAGCAGTGG94850.22705200795603642No Hit
GCTTCCCATGTACTCTGCGTTGATA94140.22535240937249626No Hit
GCGTTGATACCACTGCTTCCCATGT93680.22425126099442794No Hit
GTATCAACGCAGAGTACATGGGAAG85340.20428696213988556No Hit
TATCAACGCAGAGTACATGGGAAGC76380.18283850677577293No Hit
GGTATCAACGCAGAGTACATGGGAA75020.17958293765800581No Hit
ACTCTGCGTTGATACCACTGCTTCC69610.16663247521159402No Hit
ACGCAGAGTACTTTTTTTTTTTTTT67670.16198850161713213No Hit
GTGGTATCAACGCAGAGTACATGGG48990.11727230226427227No Hit
ACATGGGAAGCAGTGGTATCAACGC42310.10128171277406325No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCCG551.9623215E-413.8174156
GCACCGT701.0923165E-412.2136086
GACCGCG550.003071211712.0902397
TGGACCG802.8707045E-511.8743415
AAAGCCG600.005880899311.0827195
CGTACAC1053.4724853E-610.8565423
GGACCGC1053.4724853E-610.8565426
GTCTAGG3800.010.2612231
GAACCGT750.0026503910.13277156
AGAACCG900.00111490749.4994745
CTAATAC5100.09.4994743
ACGCCGA800.0045202119.4994737
ACCGACT800.0045202119.4994738
TGCTCCG1106.846268E-59.4994735
TATTCCG1451.7094881E-69.17190555
AGGACCG1252.7582946E-59.1194945
TAGACTG6350.08.9758815
ACAGATC4500.08.8661768
GTCTTGG5050.08.8512651
CTAGACA5600.08.8209394
Produced by FastQC (version 0.11.5)