FastQCFastQC Report
Wed 25 May 2016
SRR1294986_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294986_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2612667
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC1994227.632890069802237No Hit
CCCATGTACTCTGCGTTGATACCAC1881617.20187455959753No Hit
GAGTACATGGGAAGCAGTGGTATCA998173.8205021918216135No Hit
CATGTACTCTGCGTTGATACCACTG840053.2152968594926183No Hit
GCGTTGATACCACTGCTTCCCATGT635242.4313852473353856No Hit
ACGCAGAGTACATGGGAAGCAGTGG530382.030032912728641No Hit
TATCAACGCAGAGTACATGGGAAGC474211.8150418710076714No Hit
ACTCTGCGTTGATACCACTGCTTCC456301.746491229077414No Hit
GTATCAACGCAGAGTACATGGGAAG444311.7005994257974704No Hit
GGTATCAACGCAGAGTACATGGGAA404861.5496042932375231No Hit
GCTTCCCATGTACTCTGCGTTGATA330951.2667132856961871No Hit
GTGGTATCAACGCAGAGTACATGGG232880.8913497204197856No Hit
CAGTGGTATCAACGCAGAGTACATG227750.8717146119271993No Hit
GTACTCTGCGTTGATACCACTGCTT225800.8642509741960992No Hit
ATACCACTGCTTCCCATGTACTCTG212480.8132685872328926No Hit
GCAGTGGTATCAACGCAGAGTACAT209770.802896044539928No Hit
GCAGAGTACATGGGAAGCAGTGGTA207310.7934803784791555No Hit
GTTGATACCACTGCTTCCCATGTAC203300.7781320772987909No Hit
GGGAAGCAGTGGTATCAACGCAGAG190290.7283362173595028No Hit
CATGGGAAGCAGTGGTATCAACGCA185720.7108445125230272No Hit
ACATGGGAAGCAGTGGTATCAACGC185200.7088542091280672No Hit
TACCACTGCTTCCCATGTACTCTGC174850.6692395165553053No Hit
CTGCTTCCCATGTACTCTGCGTTGA172120.6587904237317653No Hit
GATACCACTGCTTCCCATGTACTCT168910.646504127774416No Hit
AAGCAGTGGTATCAACGCAGAGTAC168340.6443224490530175No Hit
CCACTGCTTCCCATGTACTCTGCGT157360.602296427367131No Hit
CTTCCCATGTACTCTGCGTTGATAC146230.5596962797019291No Hit
GTACATGGTAAGCAGTGGTATCAAC134630.5152972039682057No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA130960.5012502550076224No Hit
ATGGGAAGCAGTGGTATCAACGCAG126210.48306959899596846No Hit
TTCCCATGTACTCTGCGTTGATACC124030.47472563476325147No Hit
CCATGTACTCTGCGTTGATACCACT120420.4609083361943944No Hit
ACCATGTACTCTGCGTTGATACCAC108720.4161265098077941No Hit
TGATACCACTGCTTCCCATGTACTC106460.40747634505277563No Hit
ATCAACGCAGAGTACATGGGAAGCA75810.2901632699459977No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA71860.2750446191573591No Hit
GTATCAACGCAGAGTACTTTTTTTT70570.27010713573524675No Hit
GGAAGCAGTGGTATCAACGCAGAGT58520.22398568206357716No Hit
GGTAAGCAGTGGTATCAACGCAGAG57030.21828269733571098No Hit
GGTATCAACGCAGAGTACTTTTTTT56960.2180147718786971No Hit
TCCATGTACTCTGCGTTGATACCAC55620.21288591313014632No Hit
TATCAACGCAGAGTACTTTTTTTTT54760.20959425751540475No Hit
CCCCATGTACTCTGCGTTGATACCA53690.20549882552962168No Hit
GAGTACATGGTAAGCAGTGGTATCA53060.20308749641649704No Hit
GTACATGGGTAAGCAGTGGTATCAA52920.2025516455024693No Hit
GTACATGGAAAGCAGTGGTATCAAC48370.18513649079656916No Hit
GAACAAAAAAAAAAAAAAAAAAAAA47270.180926233614923No Hit
AAAAAGTACTCTGCGTTGATACCAC44860.17170194288058907No Hit
CTGCGTTGATACCACTGCTTCCCAT44520.17040059066080754No Hit
GTACTTTTTTTTTTTTTTTTTTTTT43920.16810408674354596No Hit
GCTTACCATGTACTCTGCGTTGATA42730.16354935397431056No Hit
CTCTGCGTTGATACCACTGCTTCCC42260.16175042590578897No Hit
AGTGGTATCAACGCAGAGTACATGG37530.1436463200247104No Hit
TGGGAAGCAGTGGTATCAACGCAGA36160.1384026360802965No Hit
GCGTTGATACCACTGCTTACCATGT35750.13683335840350108No Hit
GAGTACATGGAAAGCAGTGGTATCA35020.13403927863749954No Hit
ACTCTGCGTTGATACCACTGCTTAC34800.1331972272011703No Hit
TCCCATGTACTCTGCGTTGATACCA33540.12837456897492103No Hit
AAAGTACTCTGCGTTGATACCACTG32820.12561876427420715No Hit
TCAACGCAGAGTACATGGGAAGCAG32450.12420258685856254No Hit
ACGCAGAGTACTTTTTTTTTTTTTT32120.12293950970406868No Hit
ACGCAGAGTACATGGTAAGCAGTGG30900.11826995173897017No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN30810.11792547615138095No Hit
ACTGCTTCCCATGTACTCTGCGTTG30750.11769582575965479No Hit
CTGCTTACCATGTACTCTGCGTTGA28350.10850981009060856No Hit
TATCAACGCAGAGTACATGGTAAGC28320.1083949848947455No Hit
AACGCAGAGTACATGGGAAGCAGTG27530.10537125473701776No Hit
TTGATACCACTGCTTCCCATGTACT27440.10502677914942854No Hit
GCTTTCCATGTACTCTGCGTTGATA27310.10452920330068854No Hit
ATGGTAAGCAGTGGTATCAACGCAG26390.10100789729422081No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG402.764251E-416.6232389
TTTTAGG350.002163376216.2939912
GTACAAA12250.016.15091
GTCTTAG400.00523652214.2679221
TACGCAG400.00525992414.2583375
GTACATA11450.013.7902481
TACAAAA15700.013.5004882
GTAAAAA5300.013.2808331
TACATAA8800.013.1771472
TACATGA6850.012.9043642
GTGTAGA751.4604962E-512.6825971
TATTAGG1051.981607E-812.6731032
ACTATGC751.4811065E-512.6653238
GTACAAG4450.012.6113471
GCTTACA1301.9463187E-1012.4387021
TCTATAC701.0862832E-412.2204933
CGTCGAT550.003077142912.08684514
TACAAGA3400.012.0208112
AGAACAA14600.011.7833812
CTATACA657.9821935E-411.7000474
Produced by FastQC (version 0.11.5)