##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294986_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2612667
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.1720031676444	33.0	33.0	33.0	22.0	33.0
2	30.153896765259407	33.0	33.0	33.0	22.0	33.0
3	30.379029551029657	33.0	33.0	33.0	22.0	33.0
4	33.343269540282016	37.0	33.0	37.0	15.0	37.0
5	34.34913251478279	37.0	33.0	37.0	27.0	37.0
6	33.86848764117279	37.0	37.0	37.0	27.0	37.0
7	34.03422058762177	37.0	37.0	37.0	27.0	37.0
8	33.779477063093	37.0	37.0	37.0	27.0	37.0
9	33.60659242069502	37.0	37.0	37.0	22.0	37.0
10	33.7855566744633	37.0	33.0	37.0	27.0	37.0
11	33.95084792665885	37.0	37.0	37.0	27.0	37.0
12	33.975393726027846	37.0	37.0	37.0	27.0	37.0
13	33.611207245316756	37.0	33.0	37.0	22.0	37.0
14	35.063743676480776	37.0	33.0	40.0	22.0	40.0
15	35.04407412042943	37.0	33.0	40.0	22.0	40.0
16	35.08132762422459	37.0	33.0	40.0	27.0	40.0
17	35.17216047816274	37.0	33.0	40.0	27.0	40.0
18	35.014732455379885	37.0	33.0	40.0	22.0	40.0
19	34.931312716086666	37.0	33.0	40.0	22.0	40.0
20	35.08624137710623	37.0	33.0	40.0	27.0	40.0
21	35.015312705369645	37.0	33.0	40.0	27.0	40.0
22	35.095007132558415	37.0	33.0	40.0	27.0	40.0
23	35.1038314488605	37.0	33.0	40.0	27.0	40.0
24	35.24368126515932	37.0	33.0	40.0	27.0	40.0
25	34.11648595094591	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.6586961115740273
1101	2	0.6534891371821558
1101	3	0.5486022370725756
1101	4	0.593564031213397
1101	5	0.4241951767240906
1101	6	0.43199575156260295
1101	7	0.41783845728981817
1101	8	0.5689279272479553
1101	9	0.42890380407201434
1101	10	0.7235832812168823
1101	11	0.5004295999285091
1101	12	0.5655862626666419
1101	13	0.4101746881336865
1101	14	1.0420813669976354
1101	15	0.8724592926134562
1101	16	0.7686794358267832
1101	17	0.7033101364188497
1101	18	0.9016504543499906
1101	19	0.9252695416221357
1101	20	0.7988894909435018
1101	21	0.8838855013996678
1101	22	0.7773280191351546
1101	23	0.8160044414793859
1101	24	0.9303281466730482
1101	25	1.028449208658735
1102	1	0.4922377738895207
1102	2	0.5254597437032444
1102	3	0.5110749617983679
1102	4	0.6911360439498395
1102	5	0.5422673942030869
1102	6	0.4410238330436016
1102	7	0.5406080450136201
1102	8	0.5786825758323033
1102	9	0.6951895926177798
1102	10	0.9313213474471453
1102	11	0.7101587638130624
1102	12	0.7585526964900708
1102	13	0.7467699786539939
1102	14	1.2209917652825624
1102	15	1.0255428041603665
1102	16	0.9551487479656231
1102	17	0.9326509160101608
1102	18	0.9788520064694737
1102	19	0.9473562111199669
1102	20	0.9577070599436439
1102	21	0.9196396069573751
1102	22	0.9858736634349938
1102	23	1.067604882735992
1102	24	1.0235848486955135
1102	25	1.194024442424947
1103	1	0.47390311339225377
1103	2	0.5275219680299941
1103	3	0.38947773092864324
1103	4	0.5281046049109008
1103	5	0.2581246001024695
1103	6	0.15979916112070924
1103	7	0.23306552115197832
1103	8	0.2861703883165987
1103	9	0.2592948875103289
1103	10	0.46149500657464415
1103	11	0.3318885062667931
1103	12	0.2941621296217676
1103	13	0.26301148862793156
1103	14	0.7115828966597775
1103	15	0.623115568790297
1103	16	0.631634789259536
1103	17	0.49838123575290183
1103	18	0.6457000658608791
1103	19	0.6553894989037232
1103	20	0.6169440662987355
1103	21	0.6852599987492809
1103	22	0.6322036591968043
1103	23	0.7415694490974616
1103	24	0.8702739296863768
1103	25	0.9417254586065198
1104	1	0.448395244831854
1104	2	0.3893707865625302
1104	3	0.46855379098962047
1104	4	0.40152879497848204
1104	5	0.3044207071338292
1104	6	0.263599555301802
1104	7	0.2956031749138006
1104	8	0.3783303237457787
1104	9	0.38787118801747056
1104	10	0.5541351619448278
1104	11	0.46694488470646434
1104	12	0.363071136078851
1104	13	0.5854749429625201
1104	14	0.7905092830174993
1104	15	0.76894267132257
1104	16	0.7674416878590264
1104	17	0.6839020598969228
1104	18	0.844790095467495
1104	19	0.7437349737822103
1104	20	0.8546493061962366
1104	21	0.6647491878163478
1104	22	0.7602980587914345
1104	23	0.7883420106936398
1104	24	0.848630547388808
1104	25	0.8196974834752169
1105	1	0.2930062537608791
1105	2	0.33839358308883405
1105	3	0.26195797903218576
1105	4	0.24089808665252122
1105	5	0.18063253691336456
1105	6	0.2097430149434203
1105	7	0.17547632348561848
1105	8	0.26014823894125527
1105	9	0.3207603716610947
1105	10	0.4314406974748266
1105	11	0.2965115104000873
1105	12	0.4592106173332269
1105	13	0.46161295044322515
1105	14	0.6032704126977535
1105	15	0.6219231915219154
1105	16	0.5197384799561249
1105	17	0.4205741787245003
1105	18	0.53035393448625
1105	19	0.4848499256570875
1105	20	0.6024279731819746
1105	21	0.5483022944758105
1105	22	0.3436834184733826
1105	23	0.6316641023272211
1105	24	0.6460179328080855
1105	25	0.6751989850742177
1106	1	0.1481211043162034
1106	2	-0.07624497811954356
1106	3	-0.0428793413025339
1106	4	0.05423082371912358
1106	5	-0.345653562511977
1106	6	-0.27503589254686034
1106	7	-0.8614489845234132
1106	8	-0.35953984967249397
1106	9	0.04905461225685315
1106	10	-0.4883776115340126
1106	11	-0.5132446910401072
1106	12	-0.8681487622086479
1106	13	-0.41085986352048565
1106	14	-0.45913805769706784
1106	15	-0.18665261474250627
1106	16	-0.07764987176032179
1106	17	-0.24982320110912326
1106	18	-0.36455171162732114
1106	19	-0.5127175190311846
1106	20	-0.08709700453788116
1106	21	0.031175490902043634
1106	22	0.2681802917997089
1106	23	-0.43650643421700863
1106	24	0.2258355816644766
1106	25	0.20985262331280552
1107	1	-1.043282416794046
1107	2	-0.9487957214635081
1107	3	-0.8115913243023591
1107	4	-0.9427740813443819
1107	5	-1.1241098947600463
1107	6	-0.9722211004087526
1107	7	-1.098335420650713
1107	8	-1.0476996441479898
1107	9	-0.9649888727790525
1107	10	-0.9998412841399897
1107	11	-0.970962754001981
1107	12	-0.8568134045196132
1107	13	-0.8501691141917078
1107	14	-0.9138221382355383
1107	15	-1.1628818109712427
1107	16	-0.9779702798307142
1107	17	-1.167121996286518
1107	18	-1.445531662507733
1107	19	-1.1869133961301799
1107	20	-1.3027936214644171
1107	21	-1.207774740009647
1107	22	-1.3656614747402287
1107	23	-0.9545637422376245
1107	24	-0.9131217510400376
1107	25	-0.8356575267349768
1108	1	0.08547199386087101
1108	2	-0.11222222894467393
1108	3	-0.14428194078023893
1108	4	-0.19538994014583722
1108	5	-0.49421426276313696
1108	6	-0.051150257582236236
1108	7	-0.008342943209392217
1108	8	0.046155433219183806
1108	9	-0.3511381265820219
1108	10	-0.6019507199720806
1108	11	-0.2601746010120962
1108	12	-0.07487864321987558
1108	13	-0.07944992398855533
1108	14	-0.49726938974215074
1108	15	-0.14633824071930235
1108	16	-0.9456836859421074
1108	17	-0.9365606718362827
1108	18	-0.8566209577987536
1108	19	-0.10646598985321987
1108	20	-1.1044045528099744
1108	21	-0.19848432005411354
1108	22	-0.37145300508301204
1108	23	-0.23254580087518661
1108	24	-0.6304331741713654
1108	25	-1.1742888649705705
1109	1	0.0850371269444068
1109	2	0.04126041344929021
1109	3	0.088144302939142
1109	4	0.17702552457718923
1109	5	0.08738216933203091
1109	6	0.06689543623723182
1109	7	0.057151436313937154
1109	8	0.24481404700603804
1109	9	0.16361512565219982
1109	10	0.1920321476972049
1109	11	0.11258305407142899
1109	12	0.19661233721253524
1109	13	0.1341353184818317
1109	14	0.27257223315266543
1109	15	0.3490487480625575
1109	16	0.07184473011574966
1109	17	0.2634190819007216
1109	18	0.33037923040593853
1109	19	0.318884612686972
1109	20	0.2507157556176054
1109	21	0.27989495233511263
1109	22	0.3733083456564046
1109	23	0.3706140419453732
1109	24	0.4485571931699823
1109	25	0.5386593331120224
1110	1	0.01523573619006413
1110	2	-0.04189708185755592
1110	3	0.07702399901625157
1110	4	-1.1375009479387757
1110	5	-0.36185006705837
1110	6	-0.06400667348525246
1110	7	-0.01697923094324949
1110	8	0.11752029827877664
1110	9	0.08629177693651258
1110	10	-0.10489217982110688
1110	11	-0.008270471609904462
1110	12	0.1150502367706494
1110	13	0.050328793353557444
1110	14	0.13996250454895431
1110	15	0.10852274736595291
1110	16	0.17823081634073645
1110	17	0.24222855033611523
1110	18	0.3068975373311176
1110	19	0.20636971532437087
1110	20	0.17659387933443327
1110	21	0.275339781183753
1110	22	-0.35693583199248735
1110	23	0.0756334099077236
1110	24	0.29319748097979925
1110	25	0.3272434744174717
1111	1	-0.11273027929553692
1111	2	-0.2910003721484564
1111	3	-0.15092414551086364
1111	4	-0.09485589211815437
1111	5	-0.14316490770715973
1111	6	-0.11134634761794615
1111	7	-0.21827116377263422
1111	8	0.011851795210745308
1111	9	-0.06944202386443266
1111	10	-0.09667280363302666
1111	11	-0.10562842910781711
1111	12	-0.11222480497865917
1111	13	-0.34611208154258577
1111	14	-0.3295909909280468
1111	15	-0.22228381572980282
1111	16	-0.23685089792957825
1111	17	-0.21894159603528607
1111	18	-0.3338459935193825
1111	19	-0.1793262045433508
1111	20	-0.21036785426951354
1111	21	-0.3046905499568595
1111	22	-0.1879989120769281
1111	23	0.020952565980579152
1111	24	-0.0024417302125954166
1111	25	0.09272162153450125
1112	1	-0.2266533805451303
1112	2	-0.4125606765981047
1112	3	-0.1847610487057878
1112	4	-0.26683906704421645
1112	5	-0.07066302733313279
1112	6	-0.16183246703879206
1112	7	-0.15084554538599804
1112	8	-0.09597430010712316
1112	9	-0.1755055890535857
1112	10	0.09998696832888498
1112	11	-0.15557906928970056
1112	12	-0.010552233652653342
1112	13	-0.06433023484434841
1112	14	-0.16250469458109507
1112	15	-0.26121022900182567
1112	16	-0.15562504364147145
1112	17	-0.2680477773375287
1112	18	-0.24834175413067783
1112	19	-0.0629815875413513
1112	20	-0.8739997112243358
1112	21	-1.44737482661116
1112	22	-1.6785997129453207
1112	23	-0.539618341214414
1112	24	-0.28310122866555787
1112	25	-1.5499139363382852
1113	1	-0.22933259589785138
1113	2	-0.29748812453784623
1113	3	-0.30262994967568346
1113	4	-0.535896464306262
1113	5	-0.3489943905858226
1113	6	-0.13310088904461992
1113	7	-0.25711539442014697
1113	8	-0.07334347031238764
1113	9	-0.2604224656645471
1113	10	-0.0568664867775297
1113	11	-0.1381937813453007
1113	12	-0.11644026165313193
1113	13	-0.22545254612894894
1113	14	-0.6649539885861273
1113	15	-0.6030357620555193
1113	16	-0.6394016922836556
1113	17	-0.4614632746225382
1113	18	-0.9707154156347073
1113	19	-1.294637300182309
1113	20	-1.4215710949809548
1113	21	-1.1673843913392261
1113	22	-0.5554905254701836
1113	23	-2.159115792776305
1113	24	-1.2357982717658516
1113	25	-0.6989803849216045
1114	1	-0.3902223407141854
1114	2	-0.5030182905865956
1114	3	-0.4507978746380701
1114	4	-0.5765035922362358
1114	5	-0.8685006710809304
1114	6	-0.5751211210197837
1114	7	-0.5074977080249781
1114	8	-0.22317686454523056
1114	9	-1.3357912509477785
1114	10	-0.37693421958773854
1114	11	-0.44217296136131523
1114	12	-0.5001513190628373
1114	13	-0.5627111030988274
1114	14	-0.8006044650046746
1114	15	-1.4293037712965528
1114	16	-1.3706910771554277
1114	17	-1.6732992370299797
1114	18	-1.8667612230514266
1114	19	-1.8140473771000671
1114	20	-1.039724484475208
1114	21	-0.6409535126779247
1114	22	-0.46924602873649235
1114	23	-0.321790746483849
1114	24	-0.9202169991602176
1114	25	-0.6943017136960066
1115	1	-0.9491071432333769
1115	2	-0.6931478396559925
1115	3	-0.5638469304401958
1115	4	-0.7465436167505359
1115	5	-0.48253131631068413
1115	6	-0.6408654105186358
1115	7	-0.5820561541638298
1115	8	-0.49266336103973885
1115	9	-0.8802961987022968
1115	10	-0.5401183759753962
1115	11	-0.7239502563645388
1115	12	-0.885733163140344
1115	13	-0.6176758075451474
1115	14	-0.8230641426685494
1115	15	-1.618112341209958
1115	16	-1.059617406501964
1115	17	-0.9215217444569461
1115	18	-2.5405353170324503
1115	19	-1.3664632353443125
1115	20	-1.1320110068689644
1115	21	-1.0754170627101303
1115	22	-0.8595210361355825
1115	23	-0.5780058331103888
1115	24	-0.6809178986114688
1115	25	-0.6982259196560818
1116	1	-1.5955454579519497
1116	2	-0.8976963398406248
1116	3	-1.8328210696353615
1116	4	-1.1452830127903297
1116	5	-1.249359647956915
1116	6	-0.6642695642178609
1116	7	-1.5601422734286956
1116	8	-3.1600952621176646
1116	9	-1.7747937146179815
1116	10	-0.9716511782628885
1116	11	-0.8348437550363954
1116	12	-0.8919072075232819
1116	13	-1.9682825051488102
1116	14	-2.0348022082977906
1116	15	-1.6910862775180107
1116	16	-1.3333076843211273
1116	17	-1.9110671371925179
1116	18	-2.6491400201057758
1116	19	-1.646970362346316
1116	20	-1.2401612862236036
1116	21	-0.9301651836853253
1116	22	-0.6818580391336937
1116	23	-0.7536470639562509
1116	24	-0.9343614041218871
1116	25	-0.9058236473993375
1201	1	0.5507207143746946
1201	2	0.5156940088101969
1201	3	0.5571606526983679
1201	4	0.6077233553949313
1201	5	0.4159844140137636
1201	6	0.24534160638385316
1201	7	0.329367948492191
1201	8	0.3483384130325433
1201	9	0.31662558772164573
1201	10	0.6036708034259632
1201	11	0.3964388564277712
1201	12	0.43573565512875234
1201	13	0.40151605786908107
1201	14	0.8557847038886948
1201	15	0.7026828738231004
1201	16	0.7254629946430526
1201	17	0.7280501178930265
1201	18	0.7187713454696691
1201	19	0.6279324945778839
1201	20	0.6923816292226093
1201	21	0.5135353591440293
1201	22	0.6284851349621263
1201	23	0.7683994965600434
1201	24	0.8090992032323499
1201	25	0.9266095542176132
1202	1	0.740204655501131
1202	2	0.6844735057075262
1202	3	0.6906264192498277
1202	4	0.9117573645324626
1202	5	0.6422542834826999
1202	6	0.5007575507690802
1202	7	0.5274823004487246
1202	8	0.6069959206322011
1202	9	0.6195157901757824
1202	10	0.7667097330497299
1202	11	0.5872955809128442
1202	12	0.6224029678111194
1202	13	0.6447607076029556
1202	14	1.1855272666329668
1202	15	1.0405185776751509
1202	16	0.9764767009427828
1202	17	0.9652595454154493
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2210	22	0.629186156903323
2210	23	0.6733072599559904
2210	24	0.7094131257489167
2210	25	0.6835768463863658
2211	1	-0.04258080451767299
2211	2	-0.17474236860872594
2211	3	-0.11913875201680213
2211	4	0.026061928126601686
2211	5	-0.07865899185688363
2211	6	0.004878135313958865
2211	7	-0.07624853142700516
2211	8	0.13794298143892547
2211	9	0.2005055924133501
2211	10	0.24373043853894671
2211	11	0.08780271882886126
2211	12	0.06398652973506813
2211	13	-0.08211598417793908
2211	14	-0.2236467074918025
2211	15	-0.11889026400942271
2211	16	-0.23648239735215526
2211	17	0.010946802386250454
2211	18	-0.04431731135409933
2211	19	-0.08562547856499236
2211	20	-0.008518228959815133
2211	21	-0.2015297770948692
2211	22	-0.09767783213678172
2211	23	-0.11402898637968661
2211	24	-0.12479093128592922
2211	25	-0.05242509754415181
2212	1	0.02619065220223149
2212	2	-0.06087056770518373
2212	3	-0.050315713747451696
2212	4	-0.02732804196705274
2212	5	0.0714748573357511
2212	6	-0.0158694204214882
2212	7	0.11823017294960891
2212	8	0.2220518997683527
2212	9	-4.001073150092793E-4
2212	10	0.1598416452931275
2212	11	0.017322390564316947
2212	12	0.09240133782323312
2212	13	0.09009991538019335
2212	14	9.242479045212804E-5
2212	15	-0.08872316169222216
2212	16	-0.06406499025479917
2212	17	-0.06707029114785001
2212	18	0.09981007995996549
2212	19	0.023740712324851643
2212	20	0.022315217173932922
2212	21	0.03860703119217135
2212	22	-0.5747271090532564
2212	23	-0.023434263161242086
2212	24	-0.008620192195699872
2212	25	0.13208511647875554
2213	1	-0.07630115926647107
2213	2	-0.7714838440493494
2213	3	-0.2942012171076911
2213	4	-0.002185457169829874
2213	5	-0.0033960443811267282
2213	6	0.03914258928880088
2213	7	0.08983856892505315
2213	8	0.2295149461094823
2213	9	-0.04542823168060295
2213	10	-1.5259037602400412
2213	11	-0.35344649533570305
2213	12	-0.08263912171905474
2213	13	-0.11987585364606446
2213	14	-0.03375867473677374
2213	15	-0.07948835761689566
2213	16	-0.13479687469306612
2213	17	-0.047712515694449564
2213	18	-0.11004780920326596
2213	19	-0.04296611893017399
2213	20	-0.027864430109993066
2213	21	-0.0022857373918583335
2213	22	0.06031368205793086
2213	23	-0.9886288859680548
2213	24	-1.5716645601826613
2213	25	-1.5002203837814108
2214	1	-0.2766634059021946
2214	2	-0.43576406172574167
2214	3	-0.10197489058464271
2214	4	-0.10886166764209548
2214	5	-0.003091816918100676
2214	6	-0.2516676663712403
2214	7	-0.13985426035800685
2214	8	-0.06876381014058097
2214	9	-0.37242881709703823
2214	10	-0.07020754385141714
2214	11	-0.18756107225477336
2214	12	-0.2742394283349583
2214	13	-0.19750540719947907
2214	14	-0.21274423985520485
2214	15	-0.4241950410922044
2214	16	-0.56028465745257
2214	17	-0.3928909159426439
2214	18	-0.2813139383218015
2214	19	-1.0897398225114117
2214	20	-0.48253929011882946
2214	21	-0.4293007280831631
2214	22	-0.6784024969566005
2214	23	-1.8856591353037047
2214	24	-1.5655521955555471
2214	25	-1.7502147983164633
2215	1	-0.5623779685666754
2215	2	-0.39621242332299644
2215	3	-1.2294802339925006
2215	4	-1.0145295572746704
2215	5	-0.458194105011394
2215	6	-0.42595727180722065
2215	7	-0.2735455979789947
2215	8	-0.19313168862591112
2215	9	-0.3639195915397977
2215	10	-1.4696589055116789
2215	11	-0.6750356880383066
2215	12	-0.661311046963462
2215	13	-0.5847568357432138
2215	14	-1.0906823871830156
2215	15	-1.3869443395427368
2215	16	-0.9903688918549065
2215	17	-0.7050736435282019
2215	18	-0.6968129714194262
2215	19	-1.270065903673057
2215	20	-1.630846362803716
2215	21	-0.8872877294256796
2215	22	-0.585999587040881
2215	23	-0.7605196735702648
2215	24	-1.922122042741357
2215	25	-0.9045959080974413
2216	1	-0.6473455798758572
2216	2	-0.7273320478283054
2216	3	-1.587732741664503
2216	4	-0.9425457037736891
2216	5	-0.6814032998561075
2216	6	-0.5953217485619291
2216	7	-0.5490763850000207
2216	8	-0.5916766846039678
2216	9	-0.4146878340423612
2216	10	-0.4927132864883319
2216	11	-0.6550577352764293
2216	12	-0.5442241058069399
2216	13	-1.2550004544084246
2216	14	-0.9620651289423066
2216	15	-1.446032737882419
2216	16	-1.2615928878149631
2216	17	-2.043591362325955
2216	18	-1.1937475810355096
2216	19	-1.0260323486672291
2216	20	-0.8409471149868892
2216	21	-0.7345683016116737
2216	22	-0.6745510351758668
2216	23	-0.62324667153608
2216	24	-0.688139451248162
2216	25	-0.774748855482521
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	3413.0
3	421.0
4	423.0
5	379.0
6	267.0
7	173.0
8	270.0
9	515.0
10	1018.0
11	2035.0
12	3822.0
13	7270.0
14	13347.0
15	22268.0
16	29838.0
17	31522.0
18	30084.0
19	28799.0
20	27741.0
21	28331.0
22	27415.0
23	26077.0
24	26280.0
25	25829.0
26	25038.0
27	26146.0
28	27558.0
29	30943.0
30	37116.0
31	46824.0
32	62192.0
33	88946.0
34	128301.0
35	206158.0
36	378594.0
37	1217314.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.852050758189804	17.779918577099686	9.71154115665365	23.656489508056858
2	10.212430643695527	26.839921623062146	32.37485189289508	30.57279584034725
3	23.808532131836373	33.44662566052035	24.418638543783228	18.326203663860056
4	14.13963793934149	23.48848574681532	25.93307800661026	36.43879830723293
5	9.260517673313352	45.5197127115324	33.51815179491981	11.701617820234436
6	32.469618827889626	28.952658755238662	19.247672898859307	19.33004951801241
7	21.139004541614465	37.69428795055189	21.470527441621098	19.69618006621254
8	29.250513417909517	35.245209314171866	22.010189341313783	13.494087926604829
9	28.328622981606618	20.84936694100246	16.80552231203088	34.01648776536004
10	17.668895603874983	36.666176916823986	28.4533405862344	17.211586893066638
11	25.02937760889275	32.10029193519438	14.193143588240979	28.6771868676719
12	24.436781318234136	38.2000704946245	23.761214225799883	13.601933961341484
13	30.646862696359577	24.195228757568223	17.97842844824147	27.179480097830723
14	21.14271512015247	33.75602986925966	22.263752052466433	22.83750295812144
15	38.341822265123895	22.324260703589974	18.410646466575624	20.92327056471051
16	15.597240892625313	30.081097199663258	34.559986806603604	19.761675101107823
17	28.78983881724913	33.40399085223553	24.085482430286266	13.720687900229079
18	28.54002300408497	24.278399286942403	28.352208725827587	18.829368983145038
19	22.91180298028675	36.35949071330743	28.13675890267948	12.591947403726337
20	24.766072339430014	28.66213091051342	24.701372615947502	21.870424134109065
21	16.513057424749242	32.40474910277425	31.522633713291214	19.559559759185298
22	21.449969145757436	32.47964176095843	16.088387721046963	29.982001372237164
23	14.511631189298837	32.66202413721555	26.820636947267772	26.005707726217842
24	20.368136989769496	42.07398797810434	19.740880966354805	17.816994065771354
25	21.421831596922562	26.139100042882863	17.052996008594985	35.38607235159959
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	20179.0
1	20179.0
2	22573.5
3	24968.0
4	24968.0
5	24968.0
6	30646.5
7	36325.0
8	36325.0
9	36325.0
10	35020.0
11	33715.0
12	33715.0
13	33715.0
14	33993.0
15	34271.0
16	34271.0
17	34271.0
18	36894.0
19	39517.0
20	39517.0
21	39517.0
22	43182.5
23	46848.0
24	46848.0
25	46848.0
26	52999.5
27	59151.0
28	59151.0
29	59151.0
30	63815.0
31	68479.0
32	68479.0
33	68479.0
34	71650.0
35	74821.0
36	74821.0
37	74821.0
38	80573.5
39	86326.0
40	86326.0
41	86326.0
42	134136.5
43	181947.0
44	181947.0
45	181947.0
46	568601.0
47	955255.0
48	955255.0
49	955255.0
50	785735.0
51	616215.0
52	616215.0
53	616215.0
54	402176.0
55	188137.0
56	188137.0
57	188137.0
58	123552.5
59	58968.0
60	58968.0
61	58968.0
62	49308.0
63	39648.0
64	39648.0
65	39648.0
66	32140.5
67	24633.0
68	24633.0
69	24633.0
70	18959.0
71	13285.0
72	13285.0
73	13285.0
74	9903.0
75	6521.0
76	6521.0
77	6521.0
78	4528.0
79	2535.0
80	2535.0
81	2535.0
82	1600.5
83	666.0
84	666.0
85	666.0
86	410.5
87	155.0
88	155.0
89	155.0
90	115.5
91	76.0
92	76.0
93	76.0
94	45.5
95	15.0
96	15.0
97	15.0
98	13.0
99	11.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.31538653797058713
2	0.18207448557355377
3	0.2010589179562493
4	0.22241640438678176
5	0.24901757476172814
6	0.1963893599911508
7	0.19152842669961384
8	0.21545034250442172
9	0.21598619341844943
10	0.22559323480566026
11	0.20052306704222161
12	0.2057667509866355
13	0.2187419981191633
14	0.20813980503447244
15	0.19726968649276772
16	0.20293439615534625
17	0.20109719302153697
18	0.20301094628592162
19	0.2041591982445524
20	0.20044651691164622
21	0.19845621351668621
22	0.20079099249923543
23	0.20377644759167549
24	0.22034955086124638
25	0.2130007383260094
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2612667.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.141194104521265
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.52595024451607	14.265156794541758
2	11.584927998942113	4.434987110293082
3	5.023758587069342	2.8848221474804934
4	2.598782765172245	1.9897522137458576
5	1.6203873662296495	1.5508074550757853
6	1.004001679467696	1.1530674616773906
7	0.7297959977954529	0.9778416795353875
8	0.5057723807239236	0.7744869849713959
9	0.38251783222816327	0.6589663267607976
>10	1.7614034531850686	5.917451611893078
>50	0.12385945891272233	1.636842918499595
>100	0.10263122150672212	3.841542902966102
>500	0.012403772837297891	1.6358829260000196
>1k	0.014804503063871679	6.310167848314221
>5k	0.0022006693743593037	2.530469902153171
>10k+	0.0068020689752923925	49.43775371609189
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	199422	7.632890069802237	No Hit
CCCATGTACTCTGCGTTGATACCAC	188161	7.20187455959753	No Hit
GAGTACATGGGAAGCAGTGGTATCA	99817	3.8205021918216135	No Hit
CATGTACTCTGCGTTGATACCACTG	84005	3.2152968594926183	No Hit
GCGTTGATACCACTGCTTCCCATGT	63524	2.4313852473353856	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	53038	2.030032912728641	No Hit
TATCAACGCAGAGTACATGGGAAGC	47421	1.8150418710076714	No Hit
ACTCTGCGTTGATACCACTGCTTCC	45630	1.746491229077414	No Hit
GTATCAACGCAGAGTACATGGGAAG	44431	1.7005994257974704	No Hit
GGTATCAACGCAGAGTACATGGGAA	40486	1.5496042932375231	No Hit
GCTTCCCATGTACTCTGCGTTGATA	33095	1.2667132856961871	No Hit
GTGGTATCAACGCAGAGTACATGGG	23288	0.8913497204197856	No Hit
CAGTGGTATCAACGCAGAGTACATG	22775	0.8717146119271993	No Hit
GTACTCTGCGTTGATACCACTGCTT	22580	0.8642509741960992	No Hit
ATACCACTGCTTCCCATGTACTCTG	21248	0.8132685872328926	No Hit
GCAGTGGTATCAACGCAGAGTACAT	20977	0.802896044539928	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	20731	0.7934803784791555	No Hit
GTTGATACCACTGCTTCCCATGTAC	20330	0.7781320772987909	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	19029	0.7283362173595028	No Hit
CATGGGAAGCAGTGGTATCAACGCA	18572	0.7108445125230272	No Hit
ACATGGGAAGCAGTGGTATCAACGC	18520	0.7088542091280672	No Hit
TACCACTGCTTCCCATGTACTCTGC	17485	0.6692395165553053	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	17212	0.6587904237317653	No Hit
GATACCACTGCTTCCCATGTACTCT	16891	0.646504127774416	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	16834	0.6443224490530175	No Hit
CCACTGCTTCCCATGTACTCTGCGT	15736	0.602296427367131	No Hit
CTTCCCATGTACTCTGCGTTGATAC	14623	0.5596962797019291	No Hit
GTACATGGTAAGCAGTGGTATCAAC	13463	0.5152972039682057	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	13096	0.5012502550076224	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	12621	0.48306959899596846	No Hit
TTCCCATGTACTCTGCGTTGATACC	12403	0.47472563476325147	No Hit
CCATGTACTCTGCGTTGATACCACT	12042	0.4609083361943944	No Hit
ACCATGTACTCTGCGTTGATACCAC	10872	0.4161265098077941	No Hit
TGATACCACTGCTTCCCATGTACTC	10646	0.40747634505277563	No Hit
ATCAACGCAGAGTACATGGGAAGCA	7581	0.2901632699459977	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	7186	0.2750446191573591	No Hit
GTATCAACGCAGAGTACTTTTTTTT	7057	0.27010713573524675	No Hit
GGAAGCAGTGGTATCAACGCAGAGT	5852	0.22398568206357716	No Hit
GGTAAGCAGTGGTATCAACGCAGAG	5703	0.21828269733571098	No Hit
GGTATCAACGCAGAGTACTTTTTTT	5696	0.2180147718786971	No Hit
TCCATGTACTCTGCGTTGATACCAC	5562	0.21288591313014632	No Hit
TATCAACGCAGAGTACTTTTTTTTT	5476	0.20959425751540475	No Hit
CCCCATGTACTCTGCGTTGATACCA	5369	0.20549882552962168	No Hit
GAGTACATGGTAAGCAGTGGTATCA	5306	0.20308749641649704	No Hit
GTACATGGGTAAGCAGTGGTATCAA	5292	0.2025516455024693	No Hit
GTACATGGAAAGCAGTGGTATCAAC	4837	0.18513649079656916	No Hit
GAACAAAAAAAAAAAAAAAAAAAAA	4727	0.180926233614923	No Hit
AAAAAGTACTCTGCGTTGATACCAC	4486	0.17170194288058907	No Hit
CTGCGTTGATACCACTGCTTCCCAT	4452	0.17040059066080754	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	4392	0.16810408674354596	No Hit
GCTTACCATGTACTCTGCGTTGATA	4273	0.16354935397431056	No Hit
CTCTGCGTTGATACCACTGCTTCCC	4226	0.16175042590578897	No Hit
AGTGGTATCAACGCAGAGTACATGG	3753	0.1436463200247104	No Hit
TGGGAAGCAGTGGTATCAACGCAGA	3616	0.1384026360802965	No Hit
GCGTTGATACCACTGCTTACCATGT	3575	0.13683335840350108	No Hit
GAGTACATGGAAAGCAGTGGTATCA	3502	0.13403927863749954	No Hit
ACTCTGCGTTGATACCACTGCTTAC	3480	0.1331972272011703	No Hit
TCCCATGTACTCTGCGTTGATACCA	3354	0.12837456897492103	No Hit
AAAGTACTCTGCGTTGATACCACTG	3282	0.12561876427420715	No Hit
TCAACGCAGAGTACATGGGAAGCAG	3245	0.12420258685856254	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	3212	0.12293950970406868	No Hit
ACGCAGAGTACATGGTAAGCAGTGG	3090	0.11826995173897017	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	3081	0.11792547615138095	No Hit
ACTGCTTCCCATGTACTCTGCGTTG	3075	0.11769582575965479	No Hit
CTGCTTACCATGTACTCTGCGTTGA	2835	0.10850981009060856	No Hit
TATCAACGCAGAGTACATGGTAAGC	2832	0.1083949848947455	No Hit
AACGCAGAGTACATGGGAAGCAGTG	2753	0.10537125473701776	No Hit
TTGATACCACTGCTTCCCATGTACT	2744	0.10502677914942854	No Hit
GCTTTCCATGTACTCTGCGTTGATA	2731	0.10452920330068854	No Hit
ATGGTAAGCAGTGGTATCAACGCAG	2639	0.10100789729422081	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.827506528769261E-5	0.0
2	0.0	0.0	0.0	3.827506528769261E-5	0.0
3	0.0	0.0	0.0	3.827506528769261E-5	0.0
4	0.0	0.0	0.0	3.827506528769261E-5	0.0
5	0.0	0.0	0.0	3.827506528769261E-5	0.0
6	0.0	0.0	0.0	3.827506528769261E-5	0.0
7	0.0	0.0	0.0	3.827506528769261E-5	0.0
8	0.0	0.0	0.0	7.655013057538523E-5	0.0
9	0.0	0.0	0.0	7.655013057538523E-5	0.0
10	0.0	0.0	0.0	1.9137532643846307E-4	0.0
11	0.0	0.0	0.0	2.679254570138483E-4	0.0
12	0.0	0.0	0.0	3.062005223015409E-4	0.0
13	0.0	0.0	0.0	3.8275065287692614E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	40	2.764251E-4	16.623238	9
TTTTAGG	35	0.0021633762	16.293991	2
GTACAAA	1225	0.0	16.1509	1
GTCTTAG	40	0.005236522	14.267922	1
TACGCAG	40	0.005259924	14.258337	5
GTACATA	1145	0.0	13.790248	1
TACAAAA	1570	0.0	13.500488	2
GTAAAAA	530	0.0	13.280833	1
TACATAA	880	0.0	13.177147	2
TACATGA	685	0.0	12.904364	2
GTGTAGA	75	1.4604962E-5	12.682597	1
TATTAGG	105	1.981607E-8	12.673103	2
ACTATGC	75	1.4811065E-5	12.665323	8
GTACAAG	445	0.0	12.611347	1
GCTTACA	130	1.9463187E-10	12.438702	1
TCTATAC	70	1.0862832E-4	12.220493	3
CGTCGAT	55	0.0030771429	12.086845	14
TACAAGA	340	0.0	12.020811	2
AGAACAA	1460	0.0	11.783381	2
CTATACA	65	7.9821935E-4	11.700047	4
>>END_MODULE